Identification and Functional Characterization of Human CD4+CD25+ T Cells with Regulatory Properties Isolated from Peripheral Blood

doi:10.1084/jem.193.11.1285

Published online 4 June 2001.

This Article

	Full Text
	Full Text (PDF)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Jonuleit, H.
	Articles by Enk, A. H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Jonuleit, H.
	Articles by Enk, A. H.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0022-1007/2001/6/1285/ $5.00
The Journal of Experimental Medicine, Volume 193, Number 11, June 4, 2001 1285-1294

Original Article

Identification and Functional Characterization of Human CD4⁺CD25⁺ T Cells with Regulatory Properties Isolated from Peripheral Blood

Helmut Jonuleit^a, Edgar Schmitt^b, Michael Stassen^b, Andrea Tuettenberg^a, Jurgen Knop^a, and Alexander H. Enk^a
^a Department of Dermatology, University of Mainz, D-55101 Mainz, Germany
^b Institute of Immunology, University of Mainz, D-55101 Mainz, Germany

Correspondence to: Helmut Jonuleit, Dept. of Dermatology, University of Mainz, D-55101 Mainz, Langenbeckstr. 1, Germany. Tel:49-6131-173541 Fax:49-6131-175505 E-mail:jonuleit{at}hautklinik.klinik.uni-mainz.de.

A subpopulation of peripheral human CD4⁺CD25⁺ T cells that expressesCD45RO, histocompatibility leukocyte antigen DR, and intracellularcytotoxic T lymphocyte–associated antigen (CTLA) 4 doesnot expand after stimulation and markedly suppresses the expansionof conventional T cells in a contact-dependent manner. Afteractivation, CD4⁺CD25⁺ T cells express CTLA-4 on the surfacedetectable for several weeks. These cells show a G1/G0 cellcycle arrest and no production of interleukin (IL)-2, IL-4,or interferon (IFN)- ${gamma}$ on either protein or mRNA levels. The anergicstate of CD4⁺CD25⁺ T cells is not reversible by the additionof anti-CD28, anti–CTLA-4, anti–transforming growthfactor ß, or anti–IL-10 antibody. However, therefractory state of CD4⁺CD25⁺ T cells was partially reversibleby the addition of IL-2 or IL-4. These data demonstrate thathuman blood contains a resident T cell population with potentregulatory properties.

Key Words: human regulatory T cells, CD4⁺CD25⁺ T cells, CTLA-4 expression,T cell inhibition, tolerance

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Lages, C. S., Suffia, I., Velilla, P. A., Huang, B., Warshaw, G., Hildeman, D. A., Belkaid, Y., Chougnet, C. (2008). Functional Regulatory T Cells Accumulate in Aged Hosts and Promote Chronic Infectious Disease Reactivation. J. Immunol. 181: 1835-1848 [Abstract] [Full Text]
Ouabed, A., Hubert, F.-X., Chabannes, D., Gautreau, L., Heslan, M., Josien, R. (2008). Differential Control of T Regulatory Cell Proliferation and Suppressive Activity by Mature Plasmacytoid versus Conventional Spleen Dendritic Cells. J. Immunol. 180: 5862-5870 [Abstract] [Full Text]
Tilburgs, T., Roelen, D. L., van der Mast, B. J., de Groot-Swings, G. M., Kleijburg, C., Scherjon, S. A., Claas, F. H. (2008). Evidence for a Selective Migration of Fetus-Specific CD4+CD25bright Regulatory T Cells from the Peripheral Blood to the Decidua in Human Pregnancy. J. Immunol. 180: 5737-5745 [Abstract] [Full Text]
Antons, A. K., Wang, R., Oswald-Richter, K., Tseng, M., Arendt, C. W., Kalams, S. A., Unutmaz, D. (2008). Naive Precursors of Human Regulatory T Cells Require FoxP3 for Suppression and Are Susceptible to HIV Infection. J. Immunol. 180: 764-773 [Abstract] [Full Text]
Tiemessen, M. M., Jagger, A. L., Evans, H. G., van Herwijnen, M. J. C., John, S., Taams, L. S. (2007). CD4+CD25+Foxp3+ regulatory T cells induce alternative activation of human monocytes/macrophages. Proc. Natl. Acad. Sci. USA 104: 19446-19451 [Abstract] [Full Text]
Strauss, L., Bergmann, C., Gooding, W., Johnson, J. T., Whiteside, T. L. (2007). The Frequency and Suppressor Function of CD4+CD25highFoxp3+ T Cells in the Circulation of Patients with Squamous Cell Carcinoma of the Head and Neck. Clin. Cancer Res. 13: 6301-6311 [Abstract] [Full Text]
Oukka, M. (2007). Interplay between pathogenic Th17 and regulatory T cells. Ann Rheum Dis 66: iii87-iii90 [Abstract] [Full Text]
Watanabe, K., Mwinzi, P. N. M., Black, C. L., Muok, E. M. O., Karanja, D. M. S., Secor, W. E., Colley, D. G. (2007). T Regulatory Cell Levels Decrease in People Infected With Schistosoma mansoni on Effective Treatment. Am J Trop Med Hyg 77: 676-682 [Abstract] [Full Text]
Gibson, H. M., Hedgcock, C. J., Aufiero, B. M., Wilson, A. J., Hafner, M. S., Tsokos, G. C., Wong, H. K. (2007). Induction of the CTLA-4 Gene in Human Lymphocytes Is Dependent on NFAT Binding the Proximal Promoter. J. Immunol. 179: 3831-3840 [Abstract] [Full Text]
Landmeier, S., Altvater, B., Pscherer, S., Eing, B. R., Kuehn, J., Rooney, C. M., Juergens, H., Rossig, C. (2007). Gene-Engineered Varicella-Zoster Virus Reactive CD4+ Cytotoxic T Cells Exert Tumor-Specific Effector Function. Cancer Res. 67: 8335-8343 [Abstract] [Full Text]
Tanijiri, T., Shimizu, T., Uehira, K., Yokoi, T., Amuro, H., Sugimoto, H., Torii, Y., Tajima, K., Ito, T., Amakawa, R., Fukuhara, S. (2007). Hodgkin's Reed-Sternberg cell line (KM-H2) promotes a bidirectional differentiation of CD4+CD25+Foxp3+ T cells and CD4+ cytotoxic T lymphocytes from CD4+ naive T cells. J. Leukoc. Biol. 82: 576-584 [Abstract] [Full Text]
Kubach, J., Lutter, P., Bopp, T., Stoll, S., Becker, C., Huter, E., Richter, C., Weingarten, P., Warger, T., Knop, J., Mullner, S., Wijdenes, J., Schild, H., Schmitt, E., Jonuleit, H. (2007). Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function. Blood 110: 1550-1558 [Abstract] [Full Text]
Strauss, L., Bergmann, C., Szczepanski, M., Gooding, W., Johnson, J. T., Whiteside, T. L. (2007). A Unique Subset of CD4+CD25highFoxp3+ T Cells Secreting Interleukin-10 and Transforming Growth Factor-{beta}1 Mediates Suppression in the Tumor Microenvironment. Clin. Cancer Res. 13: 4345-4354 [Abstract] [Full Text]
van der Marel, A. P. J., Samsom, J. N., Greuter, M., van Berkel, L. A., O'Toole, T., Kraal, G., Mebius, R. E. (2007). Blockade of IDO Inhibits Nasal Tolerance Induction. J. Immunol. 179: 894-900 [Abstract] [Full Text]
Feger, U., Tolosa, E., Huang, Y.-H., Waschbisch, A., Biedermann, T., Melms, A., Wiendl, H. (2007). HLA-G expression defines a novel regulatory T-cell subset present in human peripheral blood and sites of inflammation. Blood 110: 568-577 [Abstract] [Full Text]
Dotan, I., Allez, M., Nakazawa, A., Brimnes, J., Schulder-Katz, M., Mayer, L. (2007). Intestinal epithelial cells from inflammatory bowel disease patients preferentially stimulate CD4+ T cells to proliferate and secrete interferon-{gamma}. Am. J. Physiol. Gastrointest. Liver Physiol. 292: G1630-G1640 [Abstract] [Full Text]
Pereira, L. E., Villinger, F., Onlamoon, N., Bryan, P., Cardona, A., Pattanapanysat, K., Mori, K., Hagen, S., Picker, L., Ansari, A. A. (2007). Simian Immunodeficiency Virus (SIV) Infection Influences the Level and Function of Regulatory T Cells in SIV-Infected Rhesus Macaques but Not SIV-Infected Sooty Mangabeys. J. Virol. 81: 4445-4456 [Abstract] [Full Text]
Baatar, D., Olkhanud, P., Sumitomo, K., Taub, D., Gress, R., Biragyn, A. (2007). Human Peripheral Blood T Regulatory Cells (Tregs), Functionally Primed CCR4+ Tregs and Unprimed CCR4- Tregs, Regulate Effector T Cells Using FasL. J. Immunol. 178: 4891-4900 [Abstract] [Full Text]
Bayry, J., Triebel, F., Kaveri, S. V., Tough, D. F. (2007). Human Dendritic Cells Acquire a Semimature Phenotype and Lymph Node Homing Potential through Interaction with CD4+CD25+ Regulatory T Cells. J. Immunol. 178: 4184-4193 [Abstract] [Full Text]
Berhanu, A., Huang, J., Watkins, S. C., Okada, H., Storkus, W. J. (2007). Treatment-Enhanced CD4+Foxp3+ Glucocorticoid-Induced TNF Receptor Family RelatedHigh Regulatory Tumor-Infiltrating T Cells Limit the Effectiveness of Cytokine-Based Immunotherapy. J. Immunol. 178: 3400-3408 [Abstract] [Full Text]
Garin, M. I., Chu, C.-C., Golshayan, D., Cernuda-Morollon, E., Wait, R., Lechler, R. I. (2007). Galectin-1: a key effector of regulation mediated by CD4+CD25+ T cells. Blood 109: 2058-2065 [Abstract] [Full Text]
Joetham, A., Takada, K., Taube, C., Miyahara, N., Matsubara, S., Koya, T., Rha, Y.-H., Dakhama, A., Gelfand, E. W. (2007). Naturally Occurring Lung CD4+CD25+ T Cell Regulation of Airway Allergic Responses Depends on IL-10 Induction of TGF-beta. J. Immunol. 178: 1433-1442 [Abstract] [Full Text]
Ralainirina, N., Poli, A., Michel, T., Poos, L., Andres, E., Hentges, F., Zimmer, J. (2007). Control of NK cell functions by CD4+CD25+ regulatory T cells. J. Leukoc. Biol. 81: 144-153 [Abstract] [Full Text]
Strauss, L., Whiteside, T. L., Knights, A., Bergmann, C., Knuth, A., Zippelius, A. (2007). Selective Survival of Naturally Occurring Human CD4+CD25+Foxp3+ Regulatory T Cells Cultured with Rapamycin. J. Immunol. 178: 320-329 [Abstract] [Full Text]
Keino, H, Takeuchi, M, Usui, Y, Hattori, T, Yamakawa, N, Kezuka, T, Sakai, J-I, Usui, M (2007). Supplementation of CD4+CD25+ regulatory T cells suppresses experimental autoimmune uveoretinitis. Br. J. Ophthalmol. 91: 105-110 [Abstract] [Full Text]
Kreijveld, E., Koenen, H. J. P. M., Hilbrands, L. B., van Hooff, H. J. P., Joosten, I. (2007). The immunosuppressive drug FK778 induces regulatory activity in stimulated human CD4+CD25- T cells. Blood 109: 244-252 [Abstract] [Full Text]
Battaglia, M., Stabilini, A., Migliavacca, B., Horejs-Hoeck, J., Kaupper, T., Roncarolo, M.-G. (2006). Rapamycin Promotes Expansion of Functional CD4+CD25+FOXP3+ Regulatory T Cells of Both Healthy Subjects and Type 1 Diabetic Patients. J. Immunol. 177: 8338-8347 [Abstract] [Full Text]
Hoffmann, P., Eder, R., Boeld, T. J., Doser, K., Piseshka, B., Andreesen, R., Edinger, M. (2006). Only the CD45RA+ subpopulation of CD4+CD25high T cells gives rise to homogeneous regulatory T-cell lines upon in vitro expansion. Blood 108: 4260-4267 [Abstract] [Full Text]
Scumpia, P. O., Delano, M. J., Kelly, K. M., O'Malley, K. A., Efron, P. A., McAuliffe, P. F., Brusko, T., Ungaro, R., Barker, T., Wynn, J. L., Atkinson, M. A., Reeves, W. H., Clare Salzler, M. J., Moldawer, L. L. (2006). Increased Natural CD4+CD25+ Regulatory T Cells and Their Suppressor Activity Do Not Contribute to Mortality in Murine Polymicrobial Sepsis. J. Immunol. 177: 7943-7949 [Abstract] [Full Text]
Mor, A., Luboshits, G., Planer, D., Keren, G., George, J. (2006). Altered status of CD4+CD25+ regulatory T cells in patients with acute coronary syndromes. Eur Heart J 27: 2530-2537 [Abstract] [Full Text]
Suarez, A, Lopez, P, Gomez, J, Gutierrez, C (2006). Enrichment of CD4+ CD25high T cell population in patients with systemic lupus erythematosus treated with glucocorticoids. Ann Rheum Dis 65: 1512-1517 [Abstract] [Full Text]
Bharat, A., Fields, R. C., Trulock, E. P., Patterson, G. A., Mohanakumar, T. (2006). Induction of IL-10 Suppressors in Lung Transplant Patients by CD4+25+ Regulatory T Cells through CTLA-4 Signaling. J. Immunol. 177: 5631-5638 [Abstract] [Full Text]
Kunzmann, S., Wright, J. R., Steinhilber, W., Kramer, B. W., Blaser, K., Speer, C. P., Schmidt-Weber, C. (2006). TGF-beta1 in SP-A preparations influence immune suppressive properties of SP-A on human CD4+ T lymphocytes. Am. J. Physiol. Lung Cell. Mol. Physiol. 291: L747-L756 [Abstract] [Full Text]
Lawson, C. A., Brown, A. K., Bejarano, V., Douglas, S. H., Burgoyne, C. H., Greenstein, A. S., Boylston, A. W., Emery, P., Ponchel, F., Isaacs, J. D. (2006). Early rheumatoid arthritis is associated with a deficit in the CD4+CD25high regulatory T cell population in peripheral blood. Rheumatology (Oxford) 45: 1210-1217 [Abstract] [Full Text]
Gandhi, M. K., Lambley, E., Duraiswamy, J., Dua, U., Smith, C., Elliott, S., Gill, D., Marlton, P., Seymour, J., Khanna, R. (2006). Expression of LAG-3 by tumor-infiltrating lymphocytes is coincident with the suppression of latent membrane antigen-specific CD8+ T-cell function in Hodgkin lymphoma patients. Blood 108: 2280-2289 [Abstract] [Full Text]
Mutis, T., van Rijn, R. S., Simonetti, E. R., Aarts-Riemens, T., Emmelot, M. E., van Bloois, L., Martens, A., Verdonck, L. F., Ebeling, S. B. (2006). Human Regulatory T Cells Control Xenogeneic Graft-versus-Host Disease Induced by Autologous T Cells in RAG2-/-{gamma}c-/- Immunodeficient Mice.. Clin. Cancer Res. 12: 5520-5525 [Abstract] [Full Text]
Lizee, G., Radvanyi, L. G., Overwijk, W. W., Hwu, P. (2006). Improving Antitumor Immune Responses by Circumventing Immunoregulatory Cells and Mechanisms.. Clin. Cancer Res. 12: 4794-4803 [Abstract] [Full Text]
Rezvani, K., Mielke, S., Ahmadzadeh, M., Kilical, Y., Savani, B. N., Zeilah, J., Keyvanfar, K., Montero, A., Hensel, N., Kurlander, R., Barrett, A. J. (2006). High donor FOXP3-positive regulatory T-cell (Treg) content is associated with a low risk of GVHD following HLA-matched allogeneic SCT. Blood 108: 1291-1297 [Abstract] [Full Text]
Lim, H. W., Broxmeyer, H. E., Kim, C. H. (2006). Regulation of Trafficking Receptor Expression in Human Forkhead Box P3+ Regulatory T Cells. J. Immunol. 177: 840-851 [Abstract] [Full Text]
Andaloussi, A. E., Lesniak, M. S. (2006). An increase in CD4+CD25+FOXP3+ regulatory T cells in tumor-infiltrating lymphocytes of human glioblastoma multiforme. Neuro Oncol 8: 234-243 [Abstract] [Full Text]
Knutson, K. L., Dang, Y., Lu, H., Lukas, J., Almand, B., Gad, E., Azeke, E., Disis, M. L. (2006). IL-2 Immunotoxin Therapy Modulates Tumor-Associated Regulatory T Cells and Leads to Lasting Immune-Mediated Rejection of Breast Cancers in neu-Transgenic Mice. J. Immunol. 177: 84-91 [Abstract] [Full Text]
Alard, P., Manirarora, J. N., Parnell, S. A., Hudkins, J. L., Clark, S. L., Kosiewicz, M. M. (2006). Deficiency in NOD Antigen-Presenting Cell Function May Be Responsible for Suboptimal CD4+CD25+ T-Cell-Mediated Regulation and Type 1 Diabetes Development in NOD Mice.. Diabetes 55: 2098-2105 [Abstract] [Full Text]
Wing, K., Fehervari, Z., Sakaguchi, S. (2006). Emerging possibilities in the development and function of regulatory T cells. Int Immunol 18: 991-1000 [Abstract] [Full Text]
Beyer, M., Kochanek, M., Giese, T., Endl, E., Weihrauch, M. R., Knolle, P. A., Classen, S., Schultze, J. L. (2006). In vivo peripheral expansion of naive CD4+CD25high FoxP3+ regulatory T cells in patients with multiple myeloma. Blood 107: 3940-3949 [Abstract] [Full Text]
Houot, R., Perrot, I., Garcia, E., Durand, I., Lebecque, S. (2006). Human CD4+CD25high Regulatory T Cells Modulate Myeloid but Not Plasmacytoid Dendritic Cells Activation. J. Immunol. 176: 5293-5298 [Abstract] [Full Text]
Nicolson, K. S., O'Neill, E. J., Sundstedt, A., Streeter, H. B., Minaee, S., Wraith, D. C. (2006). Antigen-Induced IL-10+ Regulatory T Cells Are Independent of CD25+ Regulatory Cells for Their Growth, Differentiation, and Function. J. Immunol. 176: 5329-5337 [Abstract] [Full Text]
Ahmadzadeh, M., Rosenberg, S. A. (2006). IL-2 administration increases CD4+CD25hi Foxp3+ regulatory T cells in cancer patients. Blood 107: 2409-2414 [Abstract] [Full Text]
Miyara, M., Amoura, Z., Parizot, C., Badoual, C., Dorgham, K., Trad, S., Kambouchner, M., Valeyre, D., Chapelon-Abric, C., Debre, P., Piette, J.-C., Gorochov, G. (2006). The immune paradox of sarcoidosis and regulatory T cells. J. Exp. Med. 203: 359-370 [Abstract] [Full Text]
Yu, Q., Yue, F. Y., Gu, X. X., Schwartz, H., Kovacs, C. M., Ostrowski, M. A. (2006). OX40 Ligation of CD4+ T Cells Enhances Virus-Specific CD8+ T Cell Memory Responses Independently of IL-2 and CD4+ T Regulatory Cell Inhibition. J. Immunol. 176: 2486-2495 [Abstract] [Full Text]
Elrefaei, M., Barugahare, B., Ssali, F., Mugyenyi, P., Cao, H. (2006). HIV-Specific IL-10-Positive CD8+ T Cells Are Increased in Advanced Disease and Are Associated with Decreased HIV-Specific Cytolysis. J. Immunol. 176: 1274-1280 [Abstract] [Full Text]
Asiedu, C. K., Goodwin, K. J., Balgansuren, G., Jenkins, S. M., Le Bas-Bernardet, S., Jargal, U., Neville, D. M. Jr, Thomas, J. M. (2005). Elevated T Regulatory Cells in Long-Term Stable Transplant Tolerance in Rhesus Macaques Induced by Anti-CD3 Immunotoxin and Deoxyspergualin. J. Immunol. 175: 8060-8068 [Abstract] [Full Text]
Miyara, M., Amoura, Z., Parizot, C., Badoual, C., Dorgham, K., Trad, S., Nochy, D., Debre, P., Piette, J.-C., Gorochov, G. (2005). Global Natural Regulatory T Cell Depletion in Active Systemic Lupus Erythematosus. J. Immunol. 175: 8392-8400 [Abstract] [Full Text]
Chen, Y.-Q., Shi, H.-Z., Qin, X.-J., Mo, W.-N., Liang, X.-D., Huang, Z.-X., Yang, H.-B., Wu, C. (2005). CD4+CD25+ Regulatory T Lymphocytes in Malignant Pleural Effusion. Am. J. Respir. Crit. Care Med. 172: 1434-1439 [Abstract] [Full Text]
Skapenko, A., Kalden, J. R., Lipsky, P. E., Schulze-Koops, H. (2005). The IL-4 Receptor {alpha}-Chain-Binding Cytokines, IL-4 and IL-13, Induce Forkhead Box P3-Expressing CD25+CD4+ Regulatory T Cells from CD25-CD4+ Precursors. J. Immunol. 175: 6107-6116 [Abstract] [Full Text]
Sen, Y., Yongyi, B., Yuling, H., Luokun, X., Li, H., Jie, X., Tao, D., Gang, Z., Junyan, L., Chunsong, H., Zhang, X., Youxin, J., Feili, G., Boquan, J., Jinquan, T. (2005). V{alpha}24-Invariant NKT Cells from Patients with Allergic Asthma Express CCR9 at High Frequency and Induce Th2 Bias of CD3+ T Cells upon CD226 Engagement. J. Immunol. 175: 4914-4926 [Abstract] [Full Text]
Zorn, E., Kim, H. T., Lee, S. J., Floyd, B. H., Litsa, D., Arumugarajah, S., Bellucci, R., Alyea, E. P., Antin, J. H., Soiffer, R. J., Ritz, J. (2005). Reduced frequency of FOXP3+ CD4+CD25+ regulatory T cells in patients with chronic graft-versus-host disease. Blood 106: 2903-2911 [Abstract] [Full Text]
Beyer, M., Kochanek, M., Darabi, K., Popov, A., Jensen, M., Endl, E., Knolle, P. A., Thomas, R. K., von Bergwelt-Baildon, M., Debey, S., Hallek, M., Schultze, J. L. (2005). Reduced frequencies and suppressive function of CD4+CD25hi regulatory T cells in patients with chronic lymphocytic leukemia after therapy with fludarabine. Blood 106: 2018-2025 [Abstract] [Full Text]
Mekala, D. J., Alli, R. S., Geiger, T. L. (2005). IL-10-dependent infectious tolerance after the treatment of experimental allergic encephalomyelitis with redirected CD4+CD25+ T lymphocytes. Proc. Natl. Acad. Sci. USA 102: 11817-11822 [Abstract] [Full Text]
Beyersdorf, N., Gaupp, S., Balbach, K., Schmidt, J., Toyka, K. V., Lin, C.-H., Hanke, T., Hunig, T., Kerkau, T., Gold, R. (2005). Selective targeting of regulatory T cells with CD28 superagonists allows effective therapy of experimental autoimmune encephalomyelitis. J. Exp. Med. 202: 445-455 [Abstract] [Full Text]
Baratelli, F., Lin, Y., Zhu, L., Yang, S.-C., Heuze-Vourc'h, N., Zeng, G., Reckamp, K., Dohadwala, M., Sharma, S., Dubinett, S. M. (2005). Prostaglandin E2 Induces FOXP3 Gene Expression and T Regulatory Cell Function in Human CD4+ T Cells. J. Immunol. 175: 1483-1490 [Abstract] [Full Text]
Robertson, M. J., Chang, H.-C., Pelloso, D., Kaplan, M. H. (2005). Impaired interferon-{gamma} production as a consequence of STAT4 deficiency after autologous hematopoietic stem cell transplantation for lymphoma. Blood 106: 963-970 [Abstract] [Full Text]
Sawitzki, B., Kingsley, C. I., Oliveira, V., Karim, M., Herber, M., Wood, K. J. (2005). IFN-{gamma} production by alloantigen-reactive regulatory T cells is important for their regulatory function in vivo. J. Exp. Med. 201: 1925-1935 [Abstract] [Full Text]
Koenen, H. J. P. M., Fasse, E., Joosten, I. (2005). CD27/CFSE-Based Ex Vivo Selection of Highly Suppressive Alloantigen-Specific Human Regulatory T Cells. J. Immunol. 174: 7573-7583 [Abstract] [Full Text]
Karim, M., Feng, G., Wood, K. J., Bushell, A. R. (2005). CD25+CD4+ regulatory T cells generated by exposure to a model protein antigen prevent allograft rejection: antigen-specific reactivation in vivo is critical for bystander regulation. Blood 105: 4871-4877 [Abstract] [Full Text]
Ruprecht, C. R., Gattorno, M., Ferlito, F., Gregorio, A., Martini, A., Lanzavecchia, A., Sallusto, F. (2005). Coexpression of CD25 and CD27 identifies FoxP3+ regulatory T cells in inflamed synovia. J. Exp. Med. 201: 1793-1803 [Abstract] [Full Text]
Brusko, T. M., Wasserfall, C. H., Agarwal, A., Kapturczak, M. H., Atkinson, M. A. (2005). An Integral Role for Heme Oxygenase-1 and Carbon Monoxide in Maintaining Peripheral Tolerance by CD4+CD25+ Regulatory T Cells. J. Immunol. 174: 5181-5186 [Abstract] [Full Text]
Brimnes, J., Allez, M., Dotan, I., Shao, L., Nakazawa, A., Mayer, L. (2005). Defects in CD8+ Regulatory T Cells in the Lamina Propria of Patients with Inflammatory Bowel Disease. J. Immunol. 174: 5814-5822 [Abstract] [Full Text]
Poussier, P., Ning, T., Murphy, T., Dabrowski, D., Ramanathan, S. (2005). Impaired Post-Thymic Development of Regulatory CD4+25+ T Cells Contributes to Diabetes Pathogenesis in BB Rats. J. Immunol. 174: 4081-4089 [Abstract] [Full Text]
Fischer, K., Voelkl, S., Heymann, J., Przybylski, G. K., Mondal, K., Laumer, M., Kunz-Schughart, L., Schmidt, C. A., Andreesen, R., Mackensen, A. (2005). Isolation and characterization of human antigen-specific TCR{alpha}{beta}+ CD4-CD8- double-negative regulatory T cells. Blood 105: 2828-2835 [Abstract] [Full Text]
Ormandy, L. A., Hillemann, T., Wedemeyer, H., Manns, M. P., Greten, T. F., Korangy, F. (2005). Increased Populations of Regulatory T Cells in Peripheral Blood of Patients with Hepatocellular Carcinoma. Cancer Res. 65: 2457-2464 [Abstract] [Full Text]
Bushell, A., Jones, E., Gallimore, A., Wood, K. (2005). The Generation of CD25+CD4+ Regulatory T Cells That Prevent Allograft Rejection Does Not Compromise Immunity to a Viral Pathogen. J. Immunol. 174: 3290-3297 [Abstract] [Full Text]
Bonnefoix, T., Bonnefoix, P., Perron, P., Mi, J.-Q., Ng, W. F., Lechler, R., Bensa, J.-C., Cahn, J.-Y., Leroux, D. (2005). Quantitating Effector and Regulatory T Lymphocytes in Immune Responses by Limiting Dilution Analysis Modeling. J. Immunol. 174: 3421-3431 [Abstract] [Full Text]
Walker, M. R., Carson, B. D., Nepom, G. T., Ziegler, S. F., Buckner, J. H. (2005). De novo generation of antigen-specific CD4+CD25+ regulatory T cells from human CD4+CD25- cells. Proc. Natl. Acad. Sci. USA 102: 4103-4108 [Abstract] [Full Text]
Liu, C. Y., Battaglia, M., Lee, S. H., Sun, Q.-H., Aster, R. H., Visentin, G. P. (2005). Platelet Factor 4 Differentially Modulates CD4+CD25+ (Regulatory) versus CD4+CD25- (Nonregulatory) T Cells. J. Immunol. 174: 2680-2686 [Abstract] [Full Text]
Leng, S., Xue, Q.-L., Huang, Y., Semba, R., Chaves, P., Bandeen-Roche, K., Fried, L., Walston, J. (2005). Total and Differential White Blood Cell Counts and Their Associations With Circulating Interleukin-6 Levels in Community-Dwelling Older Women. J. Gerontol. A Biol. Sci. Med. Sci. 60: 195-199 [Abstract] [Full Text]
Rao, P. E., Petrone, A. L., Ponath, P. D. (2005). Differentiation and Expansion of T Cells with Regulatory Function from Human Peripheral Lymphocytes by Stimulation in the Presence of TGF-{beta}. J. Immunol. 174: 1446-1455 [Abstract] [Full Text]
Balandina, A., Lecart, S., Dartevelle, P., Saoudi, A., Berrih-Aknin, S. (2005). Functional defect of regulatory CD4+CD25+ T cells in the thymus of patients with autoimmune myasthenia gravis. Blood 105: 735-741 [Abstract] [Full Text]
Lindley, S., Dayan, C. M., Bishop, A., Roep, B. O., Peakman, M., Tree, T. I.M. (2005). Defective Suppressor Function in CD4+CD25+ T-Cells From Patients With Type 1 Diabetes. Diabetes 54: 92-99 [Abstract] [Full Text]
Sugiyama, H., Gyulai, R., Toichi, E., Garaczi, E., Shimada, S., Stevens, S. R., McCormick, T. S., Cooper, K. D. (2005). Dysfunctional Blood and Target Tissue CD4+CD25high Regulatory T Cells in Psoriasis: Mechanism Underlying Unrestrained Pathogenic Effector T Cell Proliferation. J. Immunol. 174: 164-173 [Abstract] [Full Text]
Braat, H., van den Brande, J., van Tol, E., Hommes, D., Peppelenbosch, M., van Deventer, S. (2004). Lactobacillus rhamnosus induces peripheral hyporesponsiveness in stimulated CD4+ T cells via modulation of dendritic cell function. Am. J. Clin. Nutr. 80: 1618-1625 [Abstract] [Full Text]
van Santen, H.-M., Benoist, C., Mathis, D. (2004). Number of T Reg Cells That Differentiate Does Not Increase upon Encounter of Agonist Ligand on Thymic Epithelial Cells. J. Exp. Med. 200: 1221-1230 [Abstract] [Full Text]
Weiss, L., Donkova-Petrini, V., Caccavelli, L., Balbo, M., Carbonneil, C., Levy, Y. (2004). Human immunodeficiency virus-driven expansion of CD4+CD25+ regulatory T cells, which suppress HIV-specific CD4 T-cell responses in HIV-infected patients. Blood 104: 3249-3256 [Abstract] [Full Text]
Xystrakis, E., Dejean, A. S., Bernard, I., Druet, P., Liblau, R., Gonzalez-Dunia, D., Saoudi, A. (2004). Identification of a novel natural regulatory CD8 T-cell subset and analysis of its mechanism of regulation. Blood 104: 3294-3301 [Abstract] [Full Text]
Nardelli, D. T., Burchill, M. A., England, D. M., Torrealba, J., Callister, S. M., Schell, R. F. (2004). Association of CD4+ CD25+ T Cells with Prevention of Severe Destructive Arthritis in Borrelia burgdorferi-Vaccinated and Challenged Gamma Interferon-Deficient Mice Treated with Anti-Interleukin-17 Antibody. CVI 11: 1075-1084 [Abstract] [Full Text]
Sturm, A, Leite, A Z A, Danese, S, Krivacic, K A, West, G A, Mohr, S, Jacobberger, J W, Fiocchi, C (2004). Divergent cell cycle kinetics underlie the distinct functional capacity of mucosal T cells in Crohn's disease and ulcerative colitis. Gut 53: 1624-1631 [Abstract] [Full Text]
Hwang, K. W., Sweatt, W. B., Mashayekhi, M., Palucki, D. A., Sattar, H., Chuang, E., Alegre, M.-L. (2004). Transgenic Expression of CTLA-4 Controls Lymphoproliferation in IL-2-Deficient Mice. J. Immunol. 173: 5415-5424 [Abstract] [Full Text]
Yagi, H., Nomura, T., Nakamura, K., Yamazaki, S., Kitawaki, T., Hori, S., Maeda, M., Onodera, M., Uchiyama, T., Fujii, S., Sakaguchi, S. (2004). Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells. Int Immunol 16: 1643-1656 [Abstract] [Full Text]
Thio, C. L., Mosbruger, T. L., Kaslow, R. A., Karp, C. L., Strathdee, S. A., Vlahov, D., O'Brien, S. J., Astemborski, J., Thomas, D. L. (2004). Cytotoxic T-Lymphocyte Antigen 4 Gene and Recovery from Hepatitis B Virus Infection. J. Virol. 78: 11258-11262 [Abstract] [Full Text]
Bonnotte, B., Crittenden, M., Larmonier, N., Gough, M., Vile, R. G. (2004). MIP-3{alpha} Transfection into a Rodent Tumor Cell Line Increases Intratumoral Dendritic Cell Infiltration but Enhances (Facilitates) Tumor Growth and Decreases Immunogenicity. J. Immunol. 173: 4929-4935 [Abstract] [Full Text]
Sela, M., Mozes, E. (2004). Therapeutic vaccines in autoimmunity. Proc. Natl. Acad. Sci. USA 101: 14586-14592 [Abstract] [Full Text]
Machen, J., Harnaha, J., Lakomy, R., Styche, A., Trucco, M., Giannoukakis, N. (2004). Antisense Oligonucleotides Down-Regulating Costimulation Confer Diabetes-Preventive Properties to Nonobese Diabetic Mouse Dendritic Cells. J. Immunol. 173: 4331-4341 [Abstract] [Full Text]
Moseman, E. A., Liang, X., Dawson, A. J., Panoskaltsis-Mortari, A., Krieg, A. M., Liu, Y.-J., Blazar, B. R., Chen, W. (2004). Human Plasmacytoid Dendritic Cells Activated by CpG Oligodeoxynucleotides Induce the Generation of CD4+CD25+ Regulatory T Cells. J. Immunol. 173: 4433-4442 [Abstract] [Full Text]
Thornton, C. A., Upham, J. W., Wikstrom, M. E., Holt, B. J., White, G. P., Sharp, M. J., Sly, P. D., Holt, P. G. (2004). Functional Maturation of CD4+CD25+CTLA4+CD45RA+ T Regulatory Cells in Human Neonatal T Cell Responses to Environmental Antigens/Allergens. J. Immunol. 173: 3084-3092 [Abstract] [Full Text]
Makita, S., Kanai, T., Oshima, S., Uraushihara, K., Totsuka, T., Sawada, T., Nakamura, T., Koganei, K., Fukushima, T., Watanabe, M. (2004). CD4+CD25bright T Cells in Human Intestinal Lamina Propria as Regulatory Cells. J. Immunol. 173: 3119-3130 [Abstract] [Full Text]
Kinter, A. L., Hennessey, M., Bell, A., Kern, S., Lin, Y., Daucher, M., Planta, M., McGlaughlin, M., Jackson, R., Ziegler, S. F., Fauci, A. S. (2004). CD25+CD4+ Regulatory T Cells from the Peripheral Blood of Asymptomatic HIV-infected Individuals Regulate CD4+ and CD8+ HIV-specific T Cell Immune Responses In Vitro and Are Associated with Favorable Clinical Markers of Disease Status. J. Exp. Med. 200: 331-343 [Abstract] [Full Text]
Ehrenstein, M. R., Evans, J. G., Singh, A., Moore, S., Warnes, G., Isenberg, D. A., Mauri, C. (2004). Compromised Function of Regulatory T Cells in Rheumatoid Arthritis and Reversal by Anti-TNF{alpha} Therapy. J. Exp. Med. 200: 277-285 [Abstract] [Full Text]
Robinson, D S (2004). Regulation: the art of control? Regulatory T cells and asthma and allergy. Thorax 59: 640-643 [Full Text]
Manavalan, J. S., Kim-Schulze, S., Scotto, L., Naiyer, A. J., Vlad, G., Colombo, P. C., Marboe, C., Mancini, D., Cortesini, R., Suciu-Foca, N. (2004). Alloantigen specific CD8+CD28- FOXP3+ T suppressor cells induce ILT3+ ILT4+ tolerogenic endothelial cells, inhibiting alloreactivity. Int Immunol 16: 1055-1068 [Abstract] [Full Text]