X-linked Lymphoproliferative Disease: 2B4 Molecules Displaying Inhibitory Rather Than Activating Function Are Responsible for the Inability of Natural Killer Cells to Kill Epstein-Barr Virus-infected Cells

doi:10.1084/jem.192.3.337

Published online 31 July 2000.

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Parolini, S.
	Articles by Moretta, A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Parolini, S.
	Articles by Moretta, A.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0022-1007/2000/8/337/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 3, August 7, 2000 337-346

Original Article

X-linked Lymphoproliferative Disease: 2B4 Molecules Displaying Inhibitory Rather Than Activating Function Are Responsible for the Inability of Natural Killer Cells to Kill Epstein-Barr Virus–infected Cells

Silvia Parolini^a, Cristina Bottino^b, Michela Falco^c, Raffaella Augugliaro^b, Silvia Giliani^d, Roberta Franceschini^d, Hans D. Ochs^e, Hermann Wolf^f, Jean-Yves Bonnefoy^g, Roberto Biassoni^b, Lorenzo Moretta^b,c, Luigi D. Notarangelo^d, and Alessandro Moretta^c
^a Dipartimento di Scienze Biomediche e Biotecnologie, Università di Brescia, 25123 Brescia, Italy
^b Istituto Nazionale per la Ricerca sul Cancro, 16132 Genova, Italy
^c Dipartimento di Medicina Sperimentale, Università di Genova, 16132 Genova, Italy
^d Istituto di Medicina Molecolare Angelo Nocivelli, Clinica Pediatrica, Università di Brescia, 25123 Brescia, Italy
^e Department of Pediatrics, University of Washington, Seattle, Washington 98195-6320
^f Department of Immunology, University of Vienna, A-1090 Vienna, Austria
^g Centre d'Immunologie Pierre Fabre, F74164 St. Julien-en-Genevois, France

Correspondence to: Alessandro Moretta, Dipartimento di Medicina Sperimentale, Sezione di Istologia, Via G.B. Marsano 10, 16132 Genova, Italy. Tel:39-10-3537868 Fax:39-10-512747 E-mail:alemoret{at}unige.it.

2B4 is a surface molecule involved in activation of the naturalkiller (NK) cell–mediated cytotoxicity. It binds a proteintermed Src homology 2 domain–containing protein (SH2D1A)or signaling lymphocyte activation molecule (SLAM)-associatedprotein (SAP), which in turn has been proposed to function asa regulator of the 2B4-associated signal transduction pathway.In this study, we analyzed patients with X-linked lymphoproliferativedisease (XLP), a severe inherited immunodeficiency characterizedby critical mutations in the SH2D1A gene and by the inabilityto control Epstein-Barr virus (EBV) infections. We show that,in these patients, 2B4 not only fails to transduce triggeringsignals, but also mediates a sharp inhibition of the NK-mediatedcytolysis. Other receptors involved in NK cell triggering, includingCD16, NKp46, NKp44, and NKp30, displayed a normal functionalcapability. However, their activating function was inhibitedupon engagement of 2B4 molecules. CD48, the natural ligand of2B4, is highly expressed on the surface of EBV⁺ B cell lines.Remarkably, NK cells from XLP patients could not kill EBV⁺ Bcell lines. This failure was found to be the consequence ofinhibitory signals generated by the interaction between 2B4and CD48, as the antibody-mediated disruption of the 2B4–CD48interaction restored lysis of EBV⁺ target cells lacking humanhistocompatibility leukocyte antigen (HLA) class I molecules.In the case of autologous or allogeneic (HLA class I⁺) EBV⁺lymphoblastoid cell lines, restoration of lysis was achievedonly by the simultaneous disruption of 2B4–CD48 and NKreceptor–HLA class I interactions. Molecular analysisrevealed that 2B4 molecules isolated from either XLP or normalNK cells were identical. As expected, in XLP-NK cells, 2B4 didnot associate with SH2D1A, whereas similar to 2B4 moleculesisolated from normal NK cells, it did associate with Src homology2 domain–containing phosphatase 1.

Key Words: X-linked lymphoproliferative disease, Epstein-Barr virus infection,natural killer cell, natural cytotoxicity receptor, 2B4 molecule

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Nunez-Cruz, S., Yeo, W. C. J., Rothman, J., Ojha, P., Bassiri, H., Juntilla, M., Davidson, D., Veillette, A., Koretzky, G. A., Nichols, K. E. (2008). Differential Requirement for the SAP-Fyn Interaction during NK T Cell Development and Function. J. Immunol. 181: 2311-2320 [Abstract] [Full Text]
Apps, R., Gardner, L., Traherne, J., Male, V., Moffett, A. (2008). Natural-killer cell ligands at the maternal-fetal interface: UL-16 binding proteins, MHC class-I chain related molecules, HLA-F and CD48. Hum Reprod 0: den223v1-14 [Abstract] [Full Text]
Iannello, A., Debbeche, O., Samarani, S., Ahmad, A. (2008). Antiviral NK cell responses in HIV infection: I. NK cell receptor genes as determinants of HIV resistance and progression to AIDS. J. Leukoc. Biol. 84: 1-26 [Abstract] [Full Text]
Chlewicki, L. K., Velikovsky, C. A., Balakrishnan, V., Mariuzza, R. A., Kumar, V. (2008). Molecular Basis of the Dual Functions of 2B4 (CD244). J. Immunol. 180: 8159-8167 [Abstract] [Full Text]
Munitz, A., McBride, M. L., Bernstein, J. S., Rothenberg, M. E. (2008). A dual activation and inhibition role for the paired immunoglobulin-like receptor B in eosinophils. Blood 111: 5694-5703 [Abstract] [Full Text]
Vitale, C., Cottalasso, F., Montaldo, E., Moretta, L., Mingari, M. C. (2008). Methylprednisolone induces preferential and rapid differentiation of CD34+ cord blood precursors toward NK cells. Int Immunol 20: 565-575 [Abstract] [Full Text]
Kim, S., Sunwoo, J. B., Yang, L., Choi, T., Song, Y.-J., French, A. R., Vlahiotis, A., Piccirillo, J. F., Cella, M., Colonna, M., Mohanakumar, T., Hsu, K. C., Dupont, B., Yokoyama, W. M. (2008). HLA alleles determine differences in human natural killer cell responsiveness and potency. Proc. Natl. Acad. Sci. USA 105: 3053-3058 [Abstract] [Full Text]
Lee, J. K., Mathew, S. O., Vaidya, S. V., Kumaresan, P. R., Mathew, P. A. (2007). CS1 (CRACC, CD319) Induces Proliferation and Autocrine Cytokine Expression on Human B Lymphocytes. J. Immunol. 179: 4672-4678 [Abstract] [Full Text]
Clarkson, N. G., Simmonds, S. J., Puklavec, M. J., Brown, M. H. (2007). Direct and Indirect Interactions of the Cytoplasmic Region of CD244 (2B4) in Mice and Humans with FYN Kinase. J. Biol. Chem. 282: 25385-25394 [Abstract] [Full Text]
Chiossone, L., Vitale, C., Cottalasso, F., Moretti, S., Azzarone, B., Moretta, L., Mingari, M. C. (2007). Molecular analysis of the methylprednisolone-mediated inhibition of NK-cell function: evidence for different susceptibility of IL-2- versus IL-15-activated NK cells. Blood 109: 3767-3775 [Abstract] [Full Text]
Pappworth, I. Y., Wang, E. C., Rowe, M. (2007). The Switch from Latent to Productive Infection in Epstein-Barr Virus-Infected B Cells Is Associated with Sensitization to NK Cell Killing. J. Virol. 81: 474-482 [Abstract] [Full Text]
McCausland, M. M., Yusuf, I., Tran, H., Ono, N., Yanagi, Y., Crotty, S. (2007). SAP Regulation of Follicular Helper CD4 T Cell Development and Humoral Immunity Is Independent of SLAM and Fyn Kinase. J. Immunol. 178: 817-828 [Abstract] [Full Text]
Fauriat, C., Just-Landi, S., Mallet, F., Arnoulet, C., Sainty, D., Olive, D., Costello, R. T. (2007). Deficient expression of NCR in NK cells from acute myeloid leukemia: evolution during leukemia treatment and impact of leukemia cells in NCRdull phenotype induction. Blood 109: 323-330 [Abstract] [Full Text]
Vacca, P., Pietra, G., Falco, M., Romeo, E., Bottino, C., Bellora, F., Prefumo, F., Fulcheri, E., Venturini, P. L., Costa, M., Moretta, A., Moretta, L., Mingari, M. C. (2006). Analysis of natural killer cells isolated from human decidua: evidence that 2B4 (CD244) functions as an inhibitory receptor and blocks NK-cell function. Blood 108: 4078-4085 [Abstract] [Full Text]
Chiesa, M. D., Romagnani, C., Thiel, A., Moretta, L., Moretta, A. (2006). Multidirectional interactions are bridging human NK cells with plasmacytoid and monocyte-derived dendritic cells during innate immune responses. Blood 108: 3851-3858 [Abstract] [Full Text]
Crotty, S., McCausland, M. M., Aubert, R. D., Wherry, E. J., Ahmed, R. (2006). Hypogammaglobulinemia and exacerbated CD8 T-cell-mediated immunopathology in SAP-deficient mice with chronic LCMV infection mimics human XLP disease. Blood 108: 3085-3093 [Abstract] [Full Text]
Marcenaro, S., Gallo, F., Martini, S., Santoro, A., Griffiths, G. M., Arico, M., Moretta, L., Pende, D. (2006). Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease. Blood 108: 2316-2323 [Abstract] [Full Text]
Hare, N. J., Ma, C. S., Alvaro, F., Nichols, K. E., Tangye, S. G. (2006). Missense mutations in SH2D1A identified in patients with X-linked lymphoproliferative disease differentially affect the expression and function of SAP. Int Immunol 18: 1055-1065 [Abstract] [Full Text]
Fontana, S., Parolini, S., Vermi, W., Booth, S., Gallo, F., Donini, M., Benassi, M., Gentili, F., Ferrari, D., Notarangelo, L. D., Cavadini, P., Marcenaro, E., Dusi, S., Cassatella, M., Facchetti, F., Griffiths, G. M., Moretta, A., Notarangelo, L. D., Badolato, R. (2006). Innate immunity defects in Hermansky-Pudlak type 2 syndrome. Blood 107: 4857-4864 [Abstract] [Full Text]
Cannons, J. L., Yu, L. J., Jankovic, D., Crotty, S., Horai, R., Kirby, M., Anderson, S., Cheever, A. W., Sher, A., Schwartzberg, P. L. (2006). SAP regulates T cell-mediated help for humoral immunity by a mechanism distinct from cytokine regulation. J. Exp. Med. 203: 1551-1565 [Abstract] [Full Text]
Bhat, R., Eissmann, P., Endt, J., Hoffmann, S., Watzl, C. (2006). Fine-tuning of immune responses by SLAM-related receptors. J. Leukoc. Biol. 79: 417-424 [Abstract] [Full Text]
Stark, S., Watzl, C. (2006). 2B4 (CD244), NTB-A and CRACC (CS1) stimulate cytotoxicity but no proliferation in human NK cells. Int Immunol 18: 241-247 [Abstract] [Full Text]
Williams, H., Crawford, D. H. (2006). Epstein-Barr virus: the impact of scientific advances on clinical practice. Blood 107: 862-869 [Abstract] [Full Text]
Komori, H., Furukawa, H., Mori, S., Ito, M. R., Terada, M., Zhang, M.-C., Ishii, N., Sakuma, N., Nose, M., Ono, M. (2006). A Signal Adaptor SLAM-Associated Protein Regulates Spontaneous Autoimmunity and Fas-Dependent Lymphoproliferation in MRL-Faslpr Lupus Mice. J. Immunol. 176: 395-400 [Abstract] [Full Text]
Tassi, I., Colonna, M. (2005). The Cytotoxicity Receptor CRACC (CS-1) Recruits EAT-2 and Activates the PI3K and Phospholipase C{gamma} Signaling Pathways in Human NK Cells. J. Immunol. 175: 7996-8002 [Abstract] [Full Text]
Saborit-Villarroya, I., Del Valle, J. M., Romero, X., Esplugues, E., Lauzurica, P., Engel, P., Martin, M. (2005). The Adaptor Protein 3BP2 Binds Human CD244 and Links this Receptor to Vav Signaling, ERK Activation, and NK Cell Killing. J. Immunol. 175: 4226-4235 [Abstract] [Full Text]
Morandi, B., Costa, R., Falco, M., Parolini, S., De Maria, A., Ratto, G., Mingari, M. C., Melioli, G., Moretta, A., Ferlazzo, G. (2005). Distinctive Lack of CD48 Expression in Subsets of Human Dendritic Cells Tunes NK Cell Activation. J. Immunol. 175: 3690-3697 [Abstract] [Full Text]
Assarsson, E., Kambayashi, T., Persson, C. M., Chambers, B. J., Ljunggren, H.-G. (2005). 2B4/CD48-Mediated Regulation of Lymphocyte Activation and Function. J. Immunol. 175: 2045-2049 [Abstract] [Full Text]
Brando, C., Mukhopadhyay, S., Kovacs, E., Medina, R., Patel, P., Catina, T. L., Campbell, K. S., Santoli, D. (2005). Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104. J. Leukoc. Biol. 78: 359-371 [Abstract] [Full Text]
Mathew, S. O., Kumaresan, P. R., Lee, J. K., Huynh, V. T., Mathew, P. A. (2005). Mutational Analysis of the Human 2B4 (CD244)/CD48 Interaction: Lys68 and Glu70 in the V Domain of 2B4 Are Critical for CD48 Binding and Functional Activation of NK Cells. J. Immunol. 175: 1005-1013 [Abstract] [Full Text]
Vitale, M., Chiesa, M. D., Carlomagno, S., Pende, D., Arico, M., Moretta, L., Moretta, A. (2005). NK-dependent DC maturation is mediated by TNF{alpha} and IFN{gamma} released upon engagement of the NKp30 triggering receptor. Blood 106: 566-571 [Abstract] [Full Text]
Bloch-Queyrat, C., Fondaneche, M.-C., Chen, R., Yin, L., Relouzat, F., Veillette, A., Fischer, A., Latour, S. (2005). Regulation of natural cytotoxicity by the adaptor SAP and the Src-related kinase Fyn. J. Exp. Med. 202: 181-192 [Abstract] [Full Text]
Eissmann, P., Beauchamp, L., Wooters, J., Tilton, J. C., Long, E. O., Watzl, C. (2005). Molecular basis for positive and negative signaling by the natural killer cell receptor 2B4 (CD244). Blood 105: 4722-4729 [Abstract] [Full Text]
Dupre, L., Andolfi, G., Tangye, S. G., Clementi, R., Locatelli, F., Arico, M., Aiuti, A., Roncarolo, M.-G. (2005). SAP controls the cytolytic activity of CD8+ T cells against EBV-infected cells. Blood 105: 4383-4389 [Abstract] [Full Text]
Martin, M., Del Valle, J. M., Saborit, I., Engel, P. (2005). Identification of Grb2 As a Novel Binding Partner of the Signaling Lymphocytic Activation Molecule-Associated Protein Binding Receptor CD229. J. Immunol. 174: 5977-5986 [Abstract] [Full Text]
Aoukaty, A., Tan, R. (2005). Role for Glycogen Synthase Kinase-3 in NK Cell Cytotoxicity and X-Linked Lymphoproliferative Disease. J. Immunol. 174: 4551-4558 [Abstract] [Full Text]
Tabata, Y., Villanueva, J., Lee, S. M., Zhang, K., Kanegane, H., Miyawaki, T., Sumegi, J., Filipovich, A. H. (2005). Rapid detection of intracellular SH2D1A protein in cytotoxic lymphocytes from patients with X-linked lymphoproliferative disease and their family members. Blood 105: 3066-3071 [Abstract] [Full Text]
Chung, B., Aoukaty, A., Dutz, J., Terhorst, C., Tan, R. (2005). Cutting Edge: Signaling Lymphocytic Activation Molecule-Associated Protein Controls NKT Cell Functions. J. Immunol. 174: 3153-3157 [Abstract] [Full Text]
Vaidya, S. V., Stepp, S. E., McNerney, M. E., Lee, J.-K., Bennett, M., Lee, K.-M., Stewart, C. L., Kumar, V., Mathew, P. A. (2005). Targeted Disruption of the 2B4 Gene in Mice Reveals an In Vivo Role of 2B4 (CD244) in the Rejection of B16 Melanoma Cells. J. Immunol. 174: 800-807 [Abstract] [Full Text]
Roda-Navarro, P., Mittelbrunn, M., Ortega, M., Howie, D., Terhorst, C., Sanchez-Madrid, F., Fernandez-Ruiz, E. (2004). Dynamic Redistribution of the Activating 2B4/SAP Complex at the Cytotoxic NK Cell Immune Synapse. J. Immunol. 173: 3640-3646 [Abstract] [Full Text]
Mooney, J. M., Klem, J., Wulfing, C., Mijares, L. A., Schwartzberg, P. L., Bennett, M., Schatzle, J. D. (2004). The Murine NK Receptor 2B4 (CD244) Exhibits Inhibitory Function Independent of Signaling Lymphocytic Activation Molecule-Associated Protein Expression. J. Immunol. 173: 3953-3961 [Abstract] [Full Text]
Chen, R., Relouzat, F., Roncagalli, R., Aoukaty, A., Tan, R., Latour, S., Veillette, A. (2004). Molecular Dissection of 2B4 Signaling: Implications for Signal Transduction by SLAM-Related Receptors. Mol. Cell. Biol. 24: 5144-5156 [Abstract] [Full Text]
Yusa, S.-i., Catina, T. L., Campbell, K. S. (2004). KIR2DL5 Can Inhibit Human NK Cell Activation Via Recruitment of Src Homology Region 2-Containing Protein Tyrosine Phosphatase-2 (SHP-2). J. Immunol. 172: 7385-7392 [Abstract] [Full Text]
Sharifi, R., Sinclair, J. C., Gilmour, K. C., Arkwright, P. D., Kinnon, C., Thrasher, A. J., Gaspar, H. B. (2004). SAP mediates specific cytotoxic T-cell functions in X-linked lymphoproliferative disease. Blood 103: 3821-3827 [Abstract] [Full Text]
Veillette, A. (2004). SLAM Family Receptors Regulate Immunity with and without SAP-related Adaptors. J. Exp. Med. 199: 1175-1178 [Abstract] [Full Text]
Lee, K.-M., McNerney, M. E., Stepp, S. E., Mathew, P. A., Schatzle, J. D., Bennett, M., Kumar, V. (2004). 2B4 Acts As a Non-Major Histocompatibility Complex Binding Inhibitory Receptor on Mouse Natural Killer Cells. J. Exp. Med. 199: 1245-1254 [Abstract] [Full Text]
Lim, M. S., Elenitoba-Johnson, K. S.J. (2004). The Molecular Pathology of Primary Immunodeficiencies. J. Mol. Diagn. 6: 59-83 [Full Text]
Simarro, M., Lanyi, A., Howie, D., Poy, F., Bruggeman, J., Choi, M., Sumegi, J., Eck, M. J., Terhorst, C. (2004). SAP increases FynT kinase activity and is required for phosphorylation of SLAM and Ly9. Int Immunol 16: 727-736 [Abstract] [Full Text]
Ferlazzo, G., Munz, C. (2004). NK Cell Compartments and Their Activation by Dendritic Cells. J. Immunol. 172: 1333-1339 [Full Text]
Ferlazzo, G., Thomas, D., Lin, S.-L., Goodman, K., Morandi, B., Muller, W. A., Moretta, A., Munz, C. (2004). The Abundant NK Cells in Human Secondary Lymphoid Tissues Require Activation to Express Killer Cell Ig-Like Receptors and Become Cytolytic. J. Immunol. 172: 1455-1462 [Abstract] [Full Text]
Colomar, M., Puga, I., Lopez, M., Massot, M., Jorquera, J. I., Reina, M., Vilaro, S., Espel, E. (2003). Neutralization of Measles Virus Infectivity and Antibody-Dependent Cell-Mediated Cytotoxicity Activity against an Epstein-Barr Virus-Infected Cell Line by Intravenous Administration of Immunoglobulin G. CVI 10: 751-756 [Abstract] [Full Text]
Tangye, S. G., Nichols, K. E., Hare, N. J., van de Weerdt, B. C. M. (2003). Functional Requirements for Interactions Between CD84 and Src Homology 2 Domain-Containing Proteins and Their Contribution to Human T Cell Activation. J. Immunol. 171: 2485-2495 [Abstract] [Full Text]
Sanzone, S., Zeyda, M., Saemann, M. D., Soncini, M., Holter, W., Fritsch, G., Knapp, W., Candotti, F., Stulnig, T. M., Parolini, O. (2003). SLAM-associated Protein Deficiency Causes Imbalanced Early Signal Transduction and Blocks Downstream Activation in T Cells from X-linked Lymphoproliferative Disease Patients. J. Biol. Chem. 278: 29593-29599 [Abstract] [Full Text]
Li, C., Iosef, C., Jia, C. Y. H., Han, V. K. M., Li, S. S.-C. (2003). Dual Functional Roles for the X-linked Lymphoproliferative Syndrome Gene Product SAP/SH2D1A in Signaling through the Signaling Lymphocyte Activation Molecule (SLAM) Family of Immune Receptors. J. Biol. Chem. 278: 3852-3859 [Abstract] [Full Text]
Watzl, C., Long, E. O. (2003). Natural Killer Cell Inhibitory Receptors Block Actin Cytoskeleton-dependent Recruitment of 2B4 (CD244) to Lipid Rafts. J. Exp. Med. 197: 77-85 [Abstract] [Full Text]
Arkwright, P. D., Abinun, M., Cant, A. J. (2002). Autoimmunity in human primary immunodeficiency diseases. Blood 99: 2694-2702 [Abstract] [Full Text]
Aoukaty, A., Tan, R. (2002). Association of the X-linked Lymphoproliferative Disease Gene Product SAP/SH2D1A with 2B4, a Natural Killer Cell-activating Molecule, Is Dependent on Phosphoinositide 3-Kinase. J. Biol. Chem. 277: 13331-13337 [Abstract] [Full Text]
Sivori, S., Falco, M., Marcenaro, E., Parolini, S., Biassoni, R., Bottino, C., Moretta, L., Moretta, A. (2002). Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation. Proc. Natl. Acad. Sci. USA 99: 4526-4531 [Abstract] [Full Text]
Veillette, A. (2002). The SAP Family: A New Class of Adaptor-Like Molecules That Regulates Immune Cell Functions. Sci Signal 2002: pe8-pe8 [Abstract] [Full Text]
Howie, D., Simarro, M., Sayos, J., Guirado, M., Sancho, J., Terhorst, C. (2002). Molecular dissection of the signaling and costimulatory functions of CD150 (SLAM): CD150/SAP binding and CD150-mediated costimulation. Blood 99: 957-965 [Abstract] [Full Text]
Chuang, S. S., Kumaresan, P. R., Mathew, P. A. (2001). 2B4 (CD244)-Mediated Activation of Cytotoxicity and IFN-{gamma} Release in Human NK Cells Involves Distinct Pathways. J. Immunol. 167: 6210-6216 [Abstract] [Full Text]
Bouchon, A., Cella, M., Grierson, H. L., Cohen, J. I., Colonna, M. (2001). Cutting Edge: Activation of NK Cell-Mediated Cytotoxicity by a SAP-Independent Receptor of the CD2 Family. J. Immunol. 167: 5517-5521 [Abstract] [Full Text]
Martin, M., Romero, X., de la Fuente, M. A., Tovar, V., Zapater, N., Esplugues, E., Pizcueta, P., Bosch, J., Engel, P. (2001). CD84 Functions as a Homophilic Adhesion Molecule and Enhances IFN-{gamma} Secretion: Adhesion Is Mediated by Ig-Like Domain 1. J. Immunol. 167: 3668-3676 [Abstract] [Full Text]
Nakamura, H., Zarycki, J., Sullivan, J. L., Jung, J. U. (2001). Abnormal T Cell Receptor Signal Transduction of CD4 Th Cells in X-Linked Lymphoproliferative Syndrome. J. Immunol. 167: 2657-2665 [Abstract] [Full Text]
Sumazaki, R., Kanegane, H., Osaki, M., Fukushima, T., Tsuchida, M., Matsukura, H., Shinozaki, K., Kimura, H., Matsui, A., Miyawaki, T. (2001). SH2D1A mutations in Japanese males with severe Epstein-Barr virus-associated illnesses. Blood 98: 1268-1270 [Abstract] [Full Text]
Bottino, C., Falco, M., Parolini, S., Marcenaro, E., Augugliaro, R., Sivori, S., Landi, E., Biassoni, R., Notarangelo, L. D., Moretta, L., Moretta, A. (2001). NTB-A, a Novel SH2D1A-associated Surface Molecule Contributing to the Inability of Natural Killer Cells to Kill Epstein-Barr Virus-infected B Cells in X-linked Lymphoproliferative Disease. J. Exp. Med. 194: 235-246 [Abstract] [Full Text]
Sayos, J., Martin, M., Chen, A., Simarro, M., Howie, D., Morra, M., Engel, P., Terhorst, C. (2001). Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP. Blood 97: 3867-3874 [Abstract] [Full Text]
Czar, M. J., Kersh, E. N., Mijares, L. A., Lanier, G., Lewis, J., Yap, G., Chen, A., Sher, A., Duckett, C. S., Ahmed, R., Schwartzberg, P. L. (2001). Altered lymphocyte responses and cytokine production in mice deficient in the X-linked lymphoproliferative disease gene SH2D1A/DSHP/SAP. Proc. Natl. Acad. Sci. USA 10.1073/pnas.131193098v1 [Abstract] [Full Text]
Chuang, S. S., Pham, H.-T. K., Kumaresan, P. R., Mathew, P. A. (2001). A Prominent Role for Activator Protein-1 in the Transcription of the Human 2B4 (CD244) Gene in NK Cells. J. Immunol. 166: 6188-6195 [Abstract] [Full Text]
Shlapatska, L. M., Mikhalap, S. V., Berdova, A. G., Zelensky, O. M., Yun, T. J., Nichols, K. E., Clark, E. A., Sidorenko, S. P. (2001). CD150 Association with Either the SH2-Containing Inositol Phosphatase or the SH2-Containing Protein Tyrosine Phosphatase Is Regulated by the Adaptor Protein SH2D1A. J. Immunol. 166: 5480-5487 [Abstract] [Full Text]
Kingsbury, G. A., Feeney, L. A., Nong, Y., Calandra, S. A, Murphy, C. J., Corcoran, J. M., Wang, Y., Prabhu Das, M. R., Busfield, S. J., Fraser, C. C., Villeval, J. L. (2001). Cloning, Expression, and Function of BLAME, a Novel Member of the CD2 Family. J. Immunol. 166: 5675-5680 [Abstract] [Full Text]
Takei, M., Ishiwata, T., Mitamura, K., Fujiwara, S., Sasaki, K., Nishi, T., Kuga, T., Ookubo, T., Horie, T., Ryu, J., Ohi, H., Sawada, S. (2001). Decreased expression of signaling lymphocytic-activation molecule-associated protein (SAP) transcripts in T cells from patients with rheumatoid arthritis. Int Immunol 13: 559-565 [Abstract] [Full Text]
Tangye, S. G., Phillips, J. H., Lanier, L. L., Nichols, K. E. (2000). Cutting Edge: Functional Requirement for SAP in 2B4-Mediated Activation of Human Natural Killer Cells as Revealed by the X-Linked Lymphoproliferative Syndrome. J. Immunol. 165: 2932-2936 [Abstract] [Full Text]
Ulyanova, T., Shah, D. D., Thomas, M. L. (2001). Molecular Cloning of MIS, a Myeloid Inhibitory Siglec, That Binds Protein-tyrosine Phosphatases SHP-1 and SHP-2. J. Biol. Chem. 276: 14451-14458 [Abstract] [Full Text]
Sivori, S., Falco, M., Marcenaro, E., Parolini, S., Biassoni, R., Bottino, C., Moretta, L., Moretta, A. (2002). Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation. Proc. Natl. Acad. Sci. USA 99: 4526-4531 [Abstract] [Full Text]
Czar, M. J., Kersh, E. N., Mijares, L. A., Lanier, G., Lewis, J., Yap, G., Chen, A., Sher, A., Duckett, C. S., Ahmed, R., Schwartzberg, P. L. (2001). Altered lymphocyte responses and cytokine production in mice deficient in the X-linked lymphoproliferative disease gene SH2D1A/DSHP/SAP. Proc. Natl. Acad. Sci. USA 98: 7449-7454 [Abstract] [Full Text]