Original Article

Phosphoprotein Associated with Glycosphingolipid-enriched Microdomains (PAG), a Novel Ubiquitously Expressed Transmembrane Adaptor Protein, Binds the Protein Tyrosine Kinase Csk and Is Involved in Regulation of T Cell Activation

Tomá Brdika

, Dagmar Pavlitová

, Vladimír Koínek

, Jan ern

, Václav Hoejí

Correspondence to: Burkhart Schraven, Institute for Immunology, Immunomodulation Laboratory, Ruprecht-Karls University Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany. Tel:6221-56-4059 Fax:6221-56-5541 E-mail:m53{at}popix.urz.uni-heidelberg.de.

According to a recently proposed hypothesis, initiation of signal transduction via immunoreceptors depends on interactions of the engaged immunoreceptor with glycosphingolipid-enriched membrane microdomains (GEMs). In this study, we describe a novel GEM-associated transmembrane adaptor protein, termed phosphoprotein associated with GEMs (PAG). PAG comprises a short extracellular domain of 16 amino acids and a 397-amino acid cytoplasmic tail containing ten tyrosine residues that are likely phosphorylated by Src family kinases. In lymphoid cell lines and in resting peripheral blood /ß T cells, PAG is expressed as a constitutively tyrosine-phosphorylated protein and binds the major negative regulator of Src kinases, the tyrosine kinase Csk. After activation of peripheral blood /ß T cells, PAG becomes rapidly dephosphorylated and dissociates from Csk. Expression of PAG in COS cells results in recruitment of endogenous Csk, altered Src kinase activity, and impaired phosphorylation of Src-specific substrates. Moreover, overexpression of PAG in Jurkat cells downregulates T cell receptor–mediated activation of the transcription factor nuclear factor of activated T cells. These findings collectively suggest that in the absence of external stimuli, the PAG–Csk complex transmits negative regulatory signals and thus may help to keep resting T cells in a quiescent state.

Key Words: signal transduction, lymphocytes, Src family kinases, phosphorylation, Csk

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Fuss, H., Dubitzky, W., Downes, C. S., Kurth, M. J. (2008). Src Family Kinases and Receptors: Analysis of Three Activation Mechanisms by Dynamic Systems Modeling. Biophys. J 94: 1995-2006 [Abstract] [Full Text]  
• Dos Santos, C., Demur, C., Bardet, V., Prade-Houdellier, N., Payrastre, B., Recher, C. (2008). A critical role for Lyn in acute myeloid leukemia. Blood 111: 2269-2279 [Abstract] [Full Text]  
• Tauzin, S., Ding, H., Khatib, K., Ahmad, I., Burdevet, D., van Echten-Deckert, G., Lindquist, J. A., Schraven, B., Din, N.-u., Borisch, B., Hoessli, D. C. (2008). Oncogenic association of the Cbp/PAG adaptor protein with the Lyn tyrosine kinase in human B-NHL rafts. Blood 111: 2310-2320 [Abstract] [Full Text]  
• Solheim, S. A., Torgersen, K. M., Tasken, K., Berge, T. (2008). Regulation of FynT Function by Dual Domain Docking on PAG/Cbp. J. Biol. Chem. 283: 2773-2783 [Abstract] [Full Text]  
• Nunes, R. J., Castro, M. A. A., Goncalves, C. M., Bamberger, M., Pereira, C. F., Bismuth, G., Carmo, A. M. (2008). Protein Interactions between CD2 and Lck Are Required for the Lipid Raft Distribution of CD2. J. Immunol. 180: 988-997 [Abstract] [Full Text]  
• Chichili, G. R., Rodgers, W. (2007). Clustering of Membrane Raft Proteins by the Actin Cytoskeleton. J. Biol. Chem. 282: 36682-36691 [Abstract] [Full Text]  
• Nika, K., Tautz, L., Arimura, Y., Vang, T., Williams, S., Mustelin, T. (2007). A Weak Lck Tail Bite Is Necessary for Lck Function in T Cell Antigen Receptor Signaling. J. Biol. Chem. 282: 36000-36009 [Abstract] [Full Text]  
• Ruppelt, A., Mosenden, R., Gronholm, M., Aandahl, E. M., Tobin, D., Carlson, C. R., Abrahamsen, H., Herberg, F. W., Carpen, O., Tasken, K. (2007). Inhibition of T Cell Activation by Cyclic Adenosine 5'-Monophosphate Requires Lipid Raft Targeting of Protein Kinase A Type I by the A-Kinase Anchoring Protein Ezrin. J. Immunol. 179: 5159-5168 [Abstract] [Full Text]  
• Filby, A., Seddon, B., Kleczkowska, J., Salmond, R., Tomlinson, P., Smida, M., Lindquist, J. A., Schraven, B., Zamoyska, R. (2007). Fyn Regulates the Duration of TCR Engagement Needed for Commitment to Effector Function. J. Immunol. 179: 4635-4644 [Abstract] [Full Text]  
• Hong, H., Kitaura, J., Xiao, W., Horejsi, V., Ra, C., Lowell, C. A., Kawakami, Y., Kawakami, T. (2007). The Src family kinase Hck regulates mast cell activation by suppressing an inhibitory Src family kinase Lyn. Blood 110: 2511-2519 [Abstract] [Full Text]  
• Khanna, S., Roy, S., Park, H.-A, Sen, C. K. (2007). Regulation of c-Src Activity in Glutamate-induced Neurodegeneration. J. Biol. Chem. 282: 23482-23490 [Abstract] [Full Text]  
• Smida, M., Posevitz-Fejfar, A., Horejsi, V., Schraven, B., Lindquist, J. A. (2007). A novel negative regulatory function of the phosphoprotein associated with glycosphingolipid-enriched microdomains: blocking Ras activation. Blood 110: 596-625 [Abstract] [Full Text]  
• Suzuki, C., Kojima, N. (2007). A Cholesterol-independent Membrane Microdomain Serves as a Functional Counter-receptor for E-selectin at the Colo201 Cell Surface and Initiates Signalling on E-selectin Binding. J Biochem 142: 55-64 [Abstract] [Full Text]  
• Davidson, D., Schraven, B., Veillette, A. (2007). PAG-Associated FynT Regulates Calcium Signaling and Promotes Anergy in T Lymphocytes. Mol. Cell. Biol. 27: 1960-1973 [Abstract] [Full Text]  
• Mutch, C. M., Sanyal, R., Unruh, T. L., Grigoriou, L., Zhu, M., Zhang, W., Deans, J. P. (2007). Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19: 19-30 [Abstract] [Full Text]  
• Kitaura, J., Kawakami, Y., Maeda-Yamamoto, M., Horejsi, V., Kawakami, T. (2007). Dysregulation of Src Family Kinases in Mast Cells from Epilepsy-Resistant ASK versus Epilepsy-Prone EL Mice. J. Immunol. 178: 455-462 [Abstract] [Full Text]  
• Lieser, S. A., Shaffer, J., Adams, J. A. (2006). Src Tail Phosphorylation Is Limited by Structural Changes in the Regulatory Tyrosine Kinase Csk. J. Biol. Chem. 281: 38004-38012 [Abstract] [Full Text]  
• Resh, M. D. (2006). Palmitoylation of Ligands, Receptors, and Intracellular Signaling Molecules. Sci Signal 2006: re14-re14 [Abstract] [Full Text]  
• Ingley, E., Schneider, J. R., Payne, C. J., McCarthy, D. J., Harder, K. W., Hibbs, M. L., Klinken, S. P. (2006). Csk-binding Protein Mediates Sequential Enzymatic Down-regulation and Degradation of Lyn in Erythropoietin-stimulated Cells. J. Biol. Chem. 281: 31920-31929 [Abstract] [Full Text]  
• Choi, H., Cho, S.-Y., Schwartz, R. H., Choi, K. (2006). Dual Effects of Sprouty1 on TCR Signaling Depending on the Differentiation State of the T Cell. J. Immunol. 176: 6034-6045 [Abstract] [Full Text]  
• Kim, J.-E., White, F. M. (2006). Quantitative analysis of phosphotyrosine signaling networks triggered by CD3 and CD28 costimulation in jurkat cells.. J. Immunol. 176: 2833-2843 [Abstract] [Full Text]  
• Tedoldi, S., Paterson, J. C., Hansmann, M.-L., Natkunam, Y., Rudiger, T., Angelisova, P., Du, M. Q., Roberton, H., Roncador, G., Sanchez, L., Pozzobon, M., Masir, N., Barry, R., Pileri, S., Mason, D. Y., Marafioti, T., Horejsi, V. (2006). Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood 107: 213-221 [Abstract] [Full Text]  
• Maksumova, L., Le, H. T., Muratkhodjaev, F., Davidson, D., Veillette, A., Pallen, C. J. (2005). Protein Tyrosine Phosphatase {alpha} Regulates Fyn Activity and Cbp/PAG Phosphorylation in Thymocyte Lipid Rafts. J. Immunol. 175: 7947-7956 [Abstract] [Full Text]  
• Dobenecker, M.-W., Schmedt, C., Okada, M., Tarakhovsky, A. (2005). The Ubiquitously Expressed Csk Adaptor Protein Cbp Is Dispensable for Embryogenesis and T-Cell Development and Function. Mol. Cell. Biol. 25: 10533-10542 [Abstract] [Full Text]  
• Kasai, A., Shima, T., Okada, M. (2005). Role of Src family tyrosine kinases in the down-regulation of epidermal growth factor signaling in PC12 cells. GENES CELLS 10: 1175-1187 [Abstract] [Full Text]  
• Xu, S., Huo, J., Tan, J. E.-L., Lam, K.-P. (2005). Cbp Deficiency Alters Csk Localization in Lipid Rafts but Does Not Affect T-Cell Development. Mol. Cell. Biol. 25: 8486-8495 [Abstract] [Full Text]  
• Simeoni, L., Posevitz, V., Kolsch, U., Meinert, I., Bruyns, E., Pfeffer, K., Reinhold, D., Schraven, B. (2005). The Transmembrane Adapter Protein SIT Regulates Thymic Development and Peripheral T-Cell Functions. Mol. Cell. Biol. 25: 7557-7568 [Abstract] [Full Text]  
• Wang, Y., Horvath, O., Hamm-Baarke, A., Richelme, M., Gregoire, C., Guinamard, R., Horejsi, V., Angelisova, P., Spicka, J., Schraven, B., Malissen, B., Malissen, M. (2005). Single and Combined Deletions of the NTAL/LAB and LAT Adaptors Minimally Affect B-Cell Development and Function. Mol. Cell. Biol. 25: 4455-4465 [Abstract] [Full Text]  
• Tani-ichi, S., Maruyama, K., Kondo, N., Nagafuku, M., Kabayama, K., Inokuchi, J.-i., Shimada, Y., Ohno-Iwashita, Y., Yagita, H., Kawano, S., Kosugi, A. (2005). Structure and function of lipid rafts in human activated T cells. Int Immunol 17: 749-758 [Abstract] [Full Text]  
• Hao, S., August, A. (2005). Actin Depolymerization Transduces the Strength of B-Cell Receptor Stimulation. Mol. Biol. Cell 16: 2275-2284 [Abstract] [Full Text]  
• Li, F.-J., Tsuyama, N., Ishikawa, H., Obata, M., Abroun, S., Liu, S., Otsuyama, K.-i., Zheng, X., Ma, Z., Maki, Y., Kawano, M. M. (2005). A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood 105: 3295-3302 [Abstract] [Full Text]  
• Rahmouni, S., Vang, T., Alonso, A., Williams, S., van Stipdonk, M., Soncini, C., Moutschen, M., Schoenberger, S. P., Mustelin, T. (2005). Removal of C-Terminal Src Kinase from the Immune Synapse by a New Binding Protein. Mol. Cell. Biol. 25: 2227-2241 [Abstract] [Full Text]  
• Fawcett, V. C. J., Lorenz, U. (2005). Localization of Src Homology 2 Domain-Containing Phosphatase 1 (SHP-1) to Lipid Rafts in T Lymphocytes: Functional Implications and a Role for the SHP-1 Carboxyl Terminus. J. Immunol. 174: 2849-2859 [Abstract] [Full Text]  
• Koneru, M., Schaer, D., Monu, N., Ayala, A., Frey, A. B. (2005). Defective Proximal TCR Signaling Inhibits CD8+ Tumor-Infiltrating Lymphocyte Lytic Function. J. Immunol. 174: 1830-1840 [Abstract] [Full Text]  
• van Beek, J., van Meurs, M., 't Hart, B. A., Brok, H. P. M., Neal, J. W., Chatagner, A., Harris, C. L., Omidvar, N., Morgan, B. P., Laman, J. D., Gasque, P. (2005). Decay-Accelerating Factor (CD55) Is Expressed by Neurons in Response to Chronic but Not Acute Autoimmune Central Nervous System Inflammation Associated with Complement Activation. J. Immunol. 174: 2353-2365 [Abstract] [Full Text]  
• Cherukuri, A., Carter, R. H., Brooks, S., Bornmann, W., Finn, R., Dowd, C. S., Pierce, S. K. (2004). B Cell Signaling Is Regulated by Induced Palmitoylation of CD81. J. Biol. Chem. 279: 31973-31982 [Abstract] [Full Text]  
• Odom, S., Gomez, G., Kovarova, M., Furumoto, Y., Ryan, J. J., Wright, H. V., Gonzalez-Espinosa, C., Hibbs, M. L., Harder, K. W., Rivera, J. (2004). Negative Regulation of Immunoglobulin E-dependent Allergic Responses by Lyn Kinase. J. Exp. Med. 199: 1491-1502 [Abstract] [Full Text]  
• Jury, E C, Kabouridis, P S (2004). T-lymphocyte signalling in systemic lupus erythematosus: a lipid raft perspective. Lupus 13: 413-422 [Abstract]  
• Janssen, E., Zhu, M., Craven, B., Zhang, W. (2004). Linker for Activation of B Cells: A Functional Equivalent of a Mutant Linker for Activation of T Cells Deficient in Phospholipase C-{gamma}1 Binding. J. Immunol. 172: 6810-6819 [Abstract] [Full Text]  
• Xing, L., Donlin, L. T., Miller, R. H., Alexandropoulos, K. (2004). The Adapter Molecule Sin Regulates T-Cell-Receptor-Mediated Signal Transduction by Modulating Signaling Substrate Availability. Mol. Cell. Biol. 24: 4581-4592 [Abstract] [Full Text]  
• Filipp, D., Leung, B. L., Zhang, J., Veillette, A., Julius, M. (2004). Enrichment of Lck in Lipid Rafts Regulates Colocalized Fyn Activation and the Initiation of Proximal Signals through TCR{alpha}{beta}. J. Immunol. 172: 4266-4274 [Abstract] [Full Text]  
• Koonpaew, S., Janssen, E., Zhu, M., Zhang, W. (2004). The Importance of Three Membrane-distal Tyrosines in the Adaptor Protein NTAL/LAB. J. Biol. Chem. 279: 11229-11235 [Abstract] [Full Text]  
• Shrivastava, P., Katagiri, T., Ogimoto, M., Mizuno, K., Yakura, H. (2004). Dynamic regulation of Src-family kinases by CD45 in B cells. Blood 103: 1425-1432 [Abstract] [Full Text]  
• Matsuoka, H., Nada, S., Okada, M. (2004). Mechanism of Csk-mediated Down-regulation of Src Family Tyrosine Kinases in Epidermal Growth Factor Signaling. J. Biol. Chem. 279: 5975-5983 [Abstract] [Full Text]  
• TASKEN, K., AANDAHL, E. M. (2004). Localized Effects of cAMP Mediated by Distinct Routes of Protein Kinase A. Physiol. Rev. 84: 137-167 [Abstract] [Full Text]  
• Shima, T., Nada, S., Okada, M. (2003). Transmembrane phosphoprotein Cbp senses cell adhesion signaling mediated by Src family kinase in lipid rafts. Proc. Natl. Acad. Sci. USA 100: 14897-14902 [Abstract] [Full Text]  
• Hur, E. M., Son, M., Lee, O.-H., Choi, Y. B., Park, C., Lee, H., Yun, Y. (2003). LIME, a Novel Transmembrane Adaptor Protein, Associates with p56lck and Mediates T Cell Activation. J. Exp. Med. 198: 1463-1473 [Abstract] [Full Text]  
• Brdickova, N., Brdicka, T., Angelisova, P., Horvath, O., Spicka, J., Hilgert, I., Paces, J., Simeoni, L., Kliche, S., Merten, C., Schraven, B., Horejsi, V. (2003). LIME: A New Membrane Raft-associated Adaptor Protein Involved in CD4 and CD8 Coreceptor Signaling. J. Exp. Med. 198: 1453-1462 [Abstract] [Full Text]  
• Mukherjee, A., Arnaud, L., Cooper, J. A. (2003). Lipid-dependent Recruitment of Neuronal Src to Lipid Rafts in the Brain. J. Biol. Chem. 278: 40806-40814 [Abstract] [Full Text]  
• Vang, T., Abrahamsen, H., Myklebust, S., Horejsi, V., Tasken, K. (2003). Combined Spatial and Enzymatic Regulation of Csk by cAMP and Protein Kinase A Inhibits T Cell Receptor Signaling. J. Biol. Chem. 278: 17597-17600 [Abstract] [Full Text]  
• Abrahamsen, H., Vang, T., Tasken, K. (2003). Protein Kinase A Intersects Src Signaling in Membrane Microdomains. J. Biol. Chem. 278: 17170-17177 [Abstract] [Full Text]  
• Davidson, D., Bakinowski, M., Thomas, M. L., Horejsi, V., Veillette, A. (2003). Phosphorylation-Dependent Regulation of T-Cell Activation by PAG/Cbp, a Lipid Raft-Associated Transmembrane Adaptor. Mol. Cell. Biol. 23: 2017-2028 [Abstract] [Full Text]  
• Laethem, F. V., Liang, X., Andris, F., Urbain, J., Vandenbranden, M., Ruysschaert, J.-M., Resh, M. D., Stulnig, T. M., Leo, O. (2003). Glucocorticoids Alter the Lipid and Protein Composition of Membrane Rafts of a Murine T Cell Hybridoma. J. Immunol. 170: 2932-2939 [Abstract] [Full Text]  
• Baumgartner, M., Angelisova, P., Setterblad, N., Mooney, N., Werling, D., Horejsi, V., Langsley, G. (2003). Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes. Blood 101: 1874-1881 [Abstract] [Full Text]  
• Semac, I., Palomba, C., Kulangara, K., Klages, N., van Echten-Deckert, G., Borisch, B., Hoessli, D. C. (2003). Anti-CD20 Therapeutic Antibody Rituximab Modifies the Functional Organization of Rafts/Microdomains of B Lymphoma Cells. Cancer Res. 63: 534-540 [Abstract] [Full Text]  
• Brdicka, T., Imrich, M., Angelisova, P., Brdickova, N., Horvath, O., Spicka, J., Hilgert, I., Luskova, P., Draber, P., Novak, P., Engels, N., Wienands, J., Simeoni, L., Osterreicher, J., Aguado, E., Malissen, M., Schraven, B., Horejsi, V. (2002). Non-T Cell Activation Linker (NTAL): A Transmembrane Adaptor Protein Involved in Immunoreceptor Signaling. J. Exp. Med. 196: 1617-1626 [Abstract] [Full Text]  
• Edmonds, S. D., Ostergaard, H. L. (2002). Dynamic Association of CD45 with Detergent-Insoluble Microdomains in T Lymphocytes. J. Immunol. 169: 5036-5042 [Abstract] [Full Text]  
• Bunnell, S. C., Hong, D. I., Kardon, J. R., Yamazaki, T., McGlade, C. J., Barr, V. A., Samelson, L. E. (2002). T cell receptor ligation induces the formation of dynamically regulated signaling assemblies. J. Cell Biol. 158: 1263-1275 [Abstract] [Full Text]  
• Yasuda, K., Nagafuku, M., Shima, T., Okada, M., Yagi, T., Yamada, T., Minaki, Y., Kato, A., Tani-ichi, S., Hamaoka, T., Kosugi, A. (2002). Cutting Edge: Fyn Is Essential for Tyrosine Phosphorylation of Csk-Binding Protein/Phosphoprotein Associated with Glycolipid-Enriched Microdomains in Lipid Rafts in Resting T Cells. J. Immunol. 169: 2813-2817 [Abstract] [Full Text]  
• Zeyda, M., Staffler, G., Horejsi, V., Waldhausl, W., Stulnig, T. M. (2002). LAT Displacement from Lipid Rafts as a Molecular Mechanism for the Inhibition of T Cell Signaling by Polyunsaturated Fatty Acids. J. Biol. Chem. 277: 28418-28423 [Abstract] [Full Text]  
• Kono, H., Suzuki, T., Yamamoto, K., Okada, M., Yamamoto, T., Honda, Z.-i. (2002). Spatial Raft Coalescence Represents an Initial Step in Fc{gamma}R Signaling. J. Immunol. 169: 193-203 [Abstract] [Full Text]  
• Loreto, M. P., Berry, D. M., McGlade, C. J. (2002). Functional Cooperation between c-Cbl and Src-Like Adaptor Protein 2 in the Negative Regulation of T-Cell Receptor Signaling. Mol. Cell. Biol. 22: 4241-4255 [Abstract] [Full Text]  
• Polyak, M. J., Deans, J. P. (2002). Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood 99: 3256-3262 [Abstract] [Full Text]  
• Ogawa, A., Takayama, Y., Sakai, H., Chong, K. T., Takeuchi, S., Nakagawa, A., Nada, S., Okada, M., Tsukihara, T. (2002). Structure of the Carboxyl-terminal Src Kinase, Csk. J. Biol. Chem. 277: 14351-14354 [Abstract] [Full Text]  
• Obergfell, A., Eto, K., Mocsai, A., Buensuceso, C., Moores, S. L., Brugge, J. S., Lowell, C. A., Shattil, S. J. (2002). Coordinate interactions of Csk, Src, and Syk kinases with {alpha}IIb{beta}3 initiate integrin signaling to the cytoskeleton. J. Cell Biol. 157: 265-275 [Abstract] [Full Text]  
• Alonso, M. A., Millan, J. (2002). The role of lipid rafts in signalling and membrane trafficking in T lymphocytes. J. Cell Sci. 114: 3957-3965 [Abstract] [Full Text]  
• Ohtake, H., Ichikawa, N., Okada, M., Yamashita, T. (2002). Cutting Edge: Transmembrane Phosphoprotein Csk-Binding Protein/Phosphoprotein Associated With Glycosphingolipid-Enriched Microdomains as a Negative Feedback Regulator of Mast Cell Signaling Through the Fc{epsilon}RI. J. Immunol. 168: 2087-2090 [Abstract] [Full Text]  
• Schade, A. E., Levine, A. D. (2002). Lipid Raft Heterogeneity in Human Peripheral Blood T Lymphoblasts: A Mechanism for Regulating the Initiation of TCR Signal Transduction. J. Immunol. 168: 2233-2239 [Abstract] [Full Text]  
• Itoh, K., Sakakibara, M., Yamasaki, S., Takeuchi, A., Arase, H., Miyazaki, M., Nakajima, N., Okada, M., Saito, T. (2002). Cutting Edge: Negative Regulation of Immune Synapse Formation by Anchoring Lipid Raft to Cytoskeleton Through Cbp-EBP50-ERM Assembly. J. Immunol. 168: 541-544 [Abstract] [Full Text]  
• Tolar, P., Arudchandran, R., Draberova, L., Rivera, J., Draber, P. (2001). Structure-Function Analysis of Lyn Kinase Association with Lipid Rafts and Initiation of Early Signaling Events after Fcvarepsilon Receptor I Aggregation. Mol. Cell. Biol. 21: 8318-8328 [Abstract] [Full Text]  
• Dykstra, M., Cherukuri, A., Pierce, S. K. (2001). Rafts and synapses in the spatial organization of immune cell signaling receptors. J. Leukoc. Biol. 70: 699-707 [Abstract] [Full Text]  
• Vang, T., Torgersen, K. M., Sundvold, V., Saxena, M., Levy, F. O., Skalhegg, B. S., Hansson, V., Mustelin, T., Tasken, K. (2001). Activation of the COOH-terminal Src kinase (Csk) by cAMP-dependent Protein Kinase Inhibits Signaling through the T Cell Receptor. J. Exp. Med. 193: 497-508 [Abstract] [Full Text]  
• Wang, B., Lemay, S., Tsai, S., Veillette, A. (2001). SH2 Domain-Mediated Interaction of Inhibitory Protein Tyrosine Kinase Csk with Protein Tyrosine Phosphatase-HSCF. Mol. Cell. Biol. 21: 1077-1088 [Abstract] [Full Text]  
• Bénistant, C., Bourgaux, J.-F., Chapuis, H., Mottet, N., Roche, S., Bali, J.-P. (2001). The COOH-Terminal Src Kinase Csk Is a Tumor Antigen in Human Carcinoma. Cancer Res. 61: 1415-1420 [Abstract] [Full Text]  
• Johnson, K. G., Bromley, S. K., Dustin, M. L., Thomas, M. L. (2000). A supramolecular basis for CD45 tyrosine phosphatase regulation in sustained T cell activation. Proc. Natl. Acad. Sci. USA 97: 10138-10143 [Abstract] [Full Text]  
• Torgersen, K. M., Vang, T., Abrahamsen, H., Yaqub, S., Horejsi, V., Schraven, B., Rolstad, B., Mustelin, T., Tasken, K. (2001). Release from Tonic Inhibition of T Cell Activation through Transient Displacement of C-terminal Src Kinase (Csk) from Lipid Rafts. J. Biol. Chem. 276: 29313-29318 [Abstract] [Full Text]  
• Germain, R. N. (2001). The T Cell Receptor for Antigen: Signaling and Ligand Discrimination. J. Biol. Chem. 276: 35223-35226 [Full Text]  
• Obergfell, A., Eto, K., Mocsai, A., Buensuceso, C., Moores, S. L., Brugge, J. S., Lowell, C. A., Shattil, S. J. (2002). Coordinate interactions of Csk, Src, and Syk kinases with {alpha}IIb{beta}3 initiate integrin signaling to the cytoskeleton. J. Cell Biol. 157: 265-275 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS