CD5 Maintains Tolerance in Anergic B Cells

doi:10.1084/jem.191.5.883

Published online 6 March 2000.

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© The Rockefeller University Press, 0022-1007/2000/3/883/ $5.00
The Journal of Experimental Medicine, Volume 191, Number 5, March 6, 2000 883-890

Original Article

CD5 Maintains Tolerance in Anergic B Cells

Keli L. Hippen^a, Lina E. Tze^a, and Timothy W. Behrens^a
^a Center for Immunology, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455

Correspondence to: Keli L. Hippen, [same address]. Tel:612-625-5168 Fax:612-625-2199 E-mail:keli.l.hippen-1{at}tc.umn.edu.

Released online: 6 March 2000

Clonal anergy of autoreactive B cells is a key mechanism regulatingtolerance. Here, we show that anergic B cells express significantsurface levels of CD5, a molecule normally found on T cellsand a subset of B-1 cells. Breeding of the hen egg lysozyme(HEL) transgenic model for B cell anergy onto the CD5 null backgroundresulted in a spontaneous loss of B cell tolerance in vivo.Evidence for this included elevated levels of anti-HEL immunoglobulinM (IgM) antibodies in the serum of CD5^-/- mice transgenic forboth an HEL-specific B cell receptor (BCR) and soluble lysozyme."Anergic" B cells lacking CD5 also showed enhanced proliferativeresponses in vitro and elevated intracellular Ca²⁺ levels atrest and after IgM cross-linking. These data support the hypothesisthat CD5 negatively regulates Ig receptor signaling in anergicB cells and functions to inhibit autoimmune B cell responses.

Key Words: CD5, hen egg lysozyme, B cell, anergy, signal transduction

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