Precursor B Cell Receptor-dependent B Cell Proliferation and Differentiation Does Not Require the Bone Marrow or Fetal Liver Environment

doi:10.1084/jem.191.1.23

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© The Rockefeller University Press, 0022-1007/2000/1/23/ $5.00
The Journal of Experimental Medicine, Volume 191, Number 1, January 3, 2000 23-32

Original Article

Precursor B Cell Receptor–dependent B Cell Proliferation and Differentiation Does Not Require the Bone Marrow or Fetal Liver Environment

Antonius G. Rolink^a, Thomas Winkler^b, Fritz Melchers^a, and Jan Andersson^a
^a Basel Institute for Immunology, CH-4005 Basel, Switzerland
^b Department of Immunology, Friedrich-Alexander University, D-91054 Erlangen, Germany

Correspondence to: Antonius G. Rolink, Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland. Tel:41-61-605-1265 Fax:41-61-605-1364 E-mail:rolink{at}bii.ch.

The capacity of precursor B (pre-B) I cells from fetal liverand bone marrow to proliferate and differentiate into surfaceimmunoglobulin–positive immature B cells in vitro wasanalyzed. Both fetal liver– and bone marrow–derivedprogenitors do so in a pre-B cell receptor (pre-BCR)–dependentmanner in tissue culture medium alone, without addition of othercells or cytokines. Approximately 20% of the initial pre-B Icells enter more than one division. Analyses at the single-celllevel show that ~15% divide two to five times. Coculture ofpre-B I cells with stromal cells did not enhance proliferationor differentiation, whereas the presence of interleukin 7, especiallyin combination with stromal cells, resulted mainly in the expansionof pre-B I cells and prevented their further differentiation.Thus, the environment of fetal liver or bone marrow is not requiredfor the pre-BCR to exert its function, which is to select andexpand cells that have undergone an inframe V_H-D_HJ_H rearrangementthat produces a pre-BCR–compatible µH chain. Itappears unlikely that a ligand for the pre-BCR drives this pre-Bcell proliferation.

Key Words: B cell development, precursor B cell receptor, ${lambda}$ ₅, c-kit, bcl-2

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