The Journal of Experimental Medicine
StemCell Technologies
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© The Rockefeller University Press, 0022-1007/1999/9/669/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 5, September 6, 1999 669-680

A Novel Element Upstream of the V{gamma}2 Gene in the Murine T Cell Receptor {gamma} Locus Cooperates with the 3' Enhancer to Act as a Locus Control Region

Jeanne E. Bakera, Joonsoo Kanga, Na Xionga, Tempe Chena, Dragana Cadoa, and David H. Rauleta
a From the Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California at Berkeley, Berkeley, California 94720

Correspondence to: David H. Raulet, 485 LSA, University of California at Berkeley, Berkeley, CA 94720-3200. Tel:510-642-9521 Fax:510-642-1443 E-mail:raulet{at}uclink4.berkeley.edu.

Transgenic expression constructs were employed to identify a cis-acting transcription element in the T cell receptor (TCR)-{gamma} locus, called HsA, between the V{gamma}5 and V{gamma}2 genes. In constructs lacking the previously defined enhancer (3'EC{gamma}1), HsA supports transcription in mature but not immature T cells in a largely position-independent fashion. 3'EC{gamma}1, without HsA, supports transcription in immature and mature T cells but is subject to severe position effects. Together, the two elements support expression in immature and mature T cells in a copy number–dependent, position-independent fashion. Furthermore, HsA was necessary for consistent rearrangement of transgenic recombination substrates. These data suggest that HsA provides chromatin-opening activity and, together with 3'EC{gamma}1, constitutes a T cell–specific locus control region for the TCR-{gamma} locus.

Key Words: T cell receptor {gamma}, locus control region, V(D)J recombination, transcription, enhancer


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