J. Exp. Med., Volume 189, Number 10, May 17, 1999 1631-1638

The Role of 7 Integrins in CD8 T Cell Trafficking During an Antiviral Immune Response

By Leo Lefrançois,^* Christina M. Parker, Sara Olson,^* Werner Muller,^§ Norbert Wagner,^§ and Lynn Puddington^*

From the ^* Division of Rheumatic Diseases, University of Connecticut Health Center, Farmington, Connecticut 06037; the  Lymphocyte Biology Section, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and the ^§ Institute for Genetics, University of Cologne, 50937 Cologne, Germany

The requirement of 7 integrins for lymphocyte migration was examined during an ongoing immune response in vivo. Transgenic mice (OT-I) expressing an ovalbumin-specific major histocompatibility complex class I-restricted T cell receptor for antigen were rendered deficient in expression of all 7 integrins or only the E7 integrin. To quantitate the relative use of 7 integrins in migration in vivo, equal numbers of OT-I and OT-I-7^/ or OT-I-E^/ lymph node (LN) cells were adoptively transferred to normal mice. Although OT-I-7^/ LN cells migrated to mesenteric LN and peripheral LN as well as wild-type cells, 7 integrins were required for naive CD8 T cell and B cell migration to Peyer's patch. After infection with a recombinant virus (vesicular stomatitis virus) encoding ovalbumin, 7 integrins became critical for migration of activated CD8 T cells to the mesenteric LN and Peyer's patch. Naive CD8 T cells did not enter the lamina propria or the intestinal epithelium, and the majority of migration of activated CD8 T cells to the small and large intestinal mucosa, including the epithelium, was 7 integrin-mediated. The E7 integrin appeared to play no role in migration during a primary CD8 T cell immune response in vivo. Furthermore, despite dramatic upregulation of E7 by CD8 T cells after entry into the epithelium, long-term retention of intestinal intraepithelial lymphocytes was also E7 independent.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Jaensson, E., Uronen-Hansson, H., Pabst, O., Eksteen, B., Tian, J., Coombes, J. L., Berg, P.-L., Davidsson, T., Powrie, F., Johansson-Lindbom, B., Agace, W. W. (2008). Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. J. Exp. Med. 205: 2139-2149 [Abstract] [Full Text]  
• Wakabayashi, A., Nakagawa, Y., Shimizu, M., Moriya, K., Nishiyama, Y., Takahashi, H. (2008). Suppression of an Already Established Tumor Growing through Activated Mucosal CTLs Induced by Oral Administration of Tumor Antigen with Cholera Toxin. J. Immunol. 180: 4000-4010 [Abstract] [Full Text]  
• Kunisawa, J., Kurashima, Y., Higuchi, M., Gohda, M., Ishikawa, I., Ogahara, I., Kim, N., Shimizu, M., Kiyono, H. (2007). Sphingosine 1-phosphate dependence in the regulation of lymphocyte trafficking to the gut epithelium. J. Exp. Med. 204: 2335-2348 [Abstract] [Full Text]  
• Stenstad, H., Svensson, M., Cucak, H., Kotarsky, K., Agace, W. W. (2007). Differential homing mechanisms regulate regionalized effector CD8{alpha}beta+ T cell accumulation within the small intestine. Proc. Natl. Acad. Sci. USA 104: 10122-10127 [Abstract] [Full Text]  
• Marski, M., Ye, A. L., Abraham, C. (2007). CD18 Is Required for Intestinal T Cell Responses at Multiple Immune Checkpoints. J. Immunol. 178: 2104-2112 [Abstract] [Full Text]  
• Ueha, S., Murai, M., Yoneyama, H., Kitabatake, M., Imai, T., Shimaoka, T., Yonehara, S., Ishikawa, S., Matsushima, K. (2007). Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects. J. Leukoc. Biol. 81: 176-185 [Abstract] [Full Text]  
• Grover, A., Kim, G. J., Lizee, G., Tschoi, M., Wang, G., Wunderlich, J. R., Rosenberg, S. A., Hwang, S. T., Hwu, P. (2006). Intralymphatic dendritic cell vaccination induces tumor antigen-specific, skin-homing T lymphocytes.. Clin. Cancer Res. 12: 5801-5808 [Abstract] [Full Text]  
• Stenstad, H., Ericsson, A., Johansson-Lindbom, B., Svensson, M., Marsal, J., Mack, M., Picarella, D., Soler, D., Marquez, G., Briskin, M., Agace, W. W. (2006). Gut-associated lymphoid tissue-primed CD4+ T cells display CCR9-dependent and -independent homing to the small intestine. Blood 107: 3447-3454 [Abstract] [Full Text]  
• Annacker, O., Coombes, J. L., Malmstrom, V., Uhlig, H. H., Bourne, T., Johansson-Lindbom, B., Agace, W. W., Parker, C. M., Powrie, F. (2005). Essential role for CD103 in the T cell-mediated regulation of experimental colitis. J. Exp. Med. 202: 1051-1061 [Abstract] [Full Text]  
• Johansson-Lindbom, B., Svensson, M., Pabst, O., Palmqvist, C., Marquez, G., Forster, R., Agace, W. W. (2005). Functional specialization of gut CD103+ dendritic cells in the regulation of tissue-selective T cell homing. J. Exp. Med. 202: 1063-1073 [Abstract] [Full Text]  
• Gupta, S., Janani, R., Bin, Q., Luciw, P., Greer, C., Perri, S., Legg, H., Donnelly, J., Barnett, S., O'Hagan, D., Polo, J. M., Vajdy, M. (2005). Characterization of Human Immunodeficiency Virus Gag-Specific Gamma Interferon-Expressing Cells following Protective Mucosal Immunization with Alphavirus Replicon Particles. J. Virol. 79: 7135-7145 [Abstract] [Full Text]  
• Halary, F., Pitard, V., Dlubek, D., Krzysiek, R., de la Salle, H., Merville, P., Dromer, C., Emilie, D., Moreau, J.-F., Dechanet-Merville, J. (2005). Shared reactivity of V{delta}2neg {gamma}{delta} T cells against cytomegalovirus-infected cells and tumor intestinal epithelial cells. J. Exp. Med. 201: 1567-1578 [Abstract] [Full Text]  
• El-Asady, R., Yuan, R., Liu, K., Wang, D., Gress, R. E., Lucas, P. J., Drachenberg, C. B., Hadley, G. A. (2005). TGF-{beta}-dependent CD103 expression by CD8+ T cells promotes selective destruction of the host intestinal epithelium during graft-versus-host disease. J. Exp. Med. 201: 1647-1657 [Abstract] [Full Text]  
• Marshall, D. R., Olivas, E., Andreansky, S., La Gruta, N. L., Neale, G. A., Gutierrez, A., Wichlan, D. G., Wingo, S., Cheng, C., Doherty, P. C., Turner, S. J. (2005). Effector CD8+ T cells recovered from an influenza pneumonia differentiate to a state of focused gene expression. Proc. Natl. Acad. Sci. USA 102: 6074-6079 [Abstract] [Full Text]  
• Mancassola, R., Lacroix-Lamande, S., Barrier, M., Naciri, M., Salmon, H., Laurent, F. (2004). Increased Susceptibility of {beta}7-Integrin-Deficient Neonatal Mice in the Early Stage of Cryptosporidium parvum Infection. Infect. Immun. 72: 3634-3637 [Abstract] [Full Text]  
• Masopust, D., Vezys, V., Usherwood, E. J., Cauley, L. S., Olson, S., Marzo, A. L., Ward, R. L., Woodland, D. L., Lefrancois, L. (2004). Activated Primary and Memory CD8 T Cells Migrate to Nonlymphoid Tissues Regardless of Site of Activation or Tissue of Origin. J. Immunol. 172: 4875-4882 [Abstract] [Full Text]  
• Schluns, K. S., Nowak, E. C., Cabrera-Hernandez, A., Puddington, L., Lefrancois, L., Aguila, H. L. (2004). Distinct cell types control lymphoid subset development by means of IL-15 and IL-15 receptor {alpha} expression. Proc. Natl. Acad. Sci. USA 101: 5616-5621 [Abstract] [Full Text]  
• Dudda, J. C., Simon, J. C., Martin, S. (2004). Dendritic Cell Immunization Route Determines CD8+ T Cell Trafficking to Inflamed Skin: Role for Tissue Microenvironment and Dendritic Cells in Establishment of T Cell-Homing Subsets. J. Immunol. 172: 857-863 [Abstract] [Full Text]  
• Johansson-Lindbom, B., Svensson, M., Wurbel, M.-A., Malissen, B., Marquez, G., Agace, W. (2003). Selective Generation of Gut Tropic T Cells in Gut-associated Lymphoid Tissue (GALT): Requirement for GALT Dendritic Cells and Adjuvant. J. Exp. Med. 198: 963-969 [Abstract] [Full Text]  
• Haddad, W., Cooper, C. J., Zhang, Z., Brown, J. B., Zhu, Y., Issekutz, A., Fuss, I., Lee, H.-o., Kansas, G. S., Barrett, T. A. (2003). P-Selectin and P-Selectin Glycoprotein Ligand 1 Are Major Determinants for Th1 Cell Recruitment to Nonlymphoid Effector Sites in the Intestinal Lamina Propria. J. Exp. Med. 198: 369-377 [Abstract] [Full Text]  
• Shacklett, B. L., Cox, C. A., Sandberg, J. K., Stollman, N. H., Jacobson, M. A., Nixon, D. F. (2003). Trafficking of Human Immunodeficiency Virus Type 1-Specific CD8+ T Cells to Gut-Associated Lymphoid Tissue during Chronic Infection. J. Virol. 77: 5621-5631 [Abstract] [Full Text]  
• Prinz, M., Huber, G., Macpherson, A. J. S., Heppner, F. L., Glatzel, M., Eugster, H.-P., Wagner, N., Aguzzi, A. (2003). Oral Prion Infection Requires Normal Numbers of Peyer's Patches but Not of Enteric Lymphocytes. Am. J. Pathol. 162: 1103-1111 [Abstract] [Full Text]  
• Csencsits, K. L., Pascual, D. W. (2002). Absence of L-Selectin Delays Mucosal B Cell Responses in Nonintestinal Effector Tissues. J. Immunol. 169: 5649-5659 [Abstract] [Full Text]  
• Kilshaw, P. J., Higgins, J. M.G. (2002). Alpha E: No More Rejection?. J. Exp. Med. 196: 873-875 [Full Text]  
• Feng, Y., Wang, D., Yuan, R., Parker, C. M., Farber, D. L., Hadley, G. A. (2002). CD103 Expression Is Required for Destruction of Pancreatic Islet Allografts by CD8+ T Cells. J. Exp. Med. 196: 877-886 [Abstract] [Full Text]  
• D'Souza, W. N., Schluns, K. S., Masopust, D., Lefrancois, L. (2002). Essential Role for IL-2 in the Regulation of Antiviral Extralymphoid CD8 T Cell Responses. J. Immunol. 168: 5566-5572 [Abstract] [Full Text]  
• Suzuki, R., Nakao, A., Kanamaru, Y., Okumura, K., Ogawa, H., Ra, C. (2002). Localization of intestinal intraepithelial T lymphocytes involves regulation of {alpha}E{beta}7 expression by transforming growth factor-{beta}. Int Immunol 14: 339-345 [Abstract] [Full Text]  
• Haglund, K., Leiner, I., Kerksiek, K., Buonocore, L., Pamer, E., Rose, J. K. (2002). High-Level Primary CD8+ T-Cell Response to Human Immunodeficiency Virus Type 1 Gag and Env Generated by Vaccination with Recombinant Vesicular Stomatitis Viruses. J. Virol. 76: 2730-2738 [Abstract] [Full Text]  
• Stevceva, L., Kelsall, B., Nacsa, J., Moniuszko, M., Hel, Z., Tryniszewska, E., Franchini, G. (2002). Cervicovaginal Lamina Propria Lymphocytes: Phenotypic Characterization and Their Importance in Cytotoxic T-Lymphocyte Responses to Simian Immunodeficiency Virus SIVmac251. J. Virol. 76: 9-18 [Abstract] [Full Text]  
• Gurish, M. F., Tao, H., Abonia, J. P., Arya, A., Friend, D. S., Parker, C. M., Austen, K. F. (2001). Intestinal Mast Cell Progenitors Require CD49d{beta}7 ({alpha}4{beta}7 Integrin) for Tissue-specific Homing. J. Exp. Med. 194: 1243-1252 [Abstract] [Full Text]  
• Lefrancois, L., Vezys, V. (2001). Transgenic Mouse Model of Intestine-Specific Mucosal Injury and Repair. J Natl Cancer Inst Monogr 2001: 21-25 [Abstract] [Full Text]  
• Singbartl, K., Thatte, J., Smith, M. L., Wethmar, K., Day, K., Ley, K. (2001). A CD2-Green Fluorescence Protein-Transgenic Mouse Reveals Very Late Antigen-4-Dependent CD8+ Lymphocyte Rolling in Inflamed Venules. J. Immunol. 166: 7520-7526 [Abstract] [Full Text]  
• Rayevskaya, M. V., Frankel, F. R. (2001). Systemic Immunity and Mucosal Immunity Are Induced against Human Immunodeficiency Virus Gag Protein in Mice by a New Hyperattenuated Strain of Listeria monocytogenes. J. Virol. 75: 2786-2791 [Abstract] [Full Text]  
• Huleatt, J. W., Pilip, I., Kerksiek, K., Pamer, E. G. (2001). Intestinal and Splenic T Cell Responses to Enteric Listeria monocytogenes Infection: Distinct Repertoires of Responding CD8 T Lymphocytes. J. Immunol. 166: 4065-4073 [Abstract] [Full Text]  
• Strauch, U. G., Mueller, R. C., Li, X. Y., Cernadas, M., Higgins, J. M. G., Binion, D. G., Parker, C. M. (2001). Integrin {{alpha}}E(CD103){{beta}}7 Mediates Adhesion to Intestinal Microvascular Endothelial Cell Lines Via an E-Cadherin-Independent Interaction. J. Immunol. 166: 3506-3514 [Abstract] [Full Text]  
• Masopust, D., Jiang, J., Shen, H., Lefrancois, L. (2001). Direct Analysis of the Dynamics of the Intestinal Mucosa CD8 T Cell Response to Systemic Virus Infection. J. Immunol. 166: 2348-2356 [Abstract] [Full Text]  
• Arstila, T., Arstila, T. P., Calbo, S., Selz, F., Malassis-Seris, M., Vassalli, P., Kourilsky, P., Guy-Grand, D. (2000). Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph. J. Exp. Med. 191: 823-834 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS