Contribution of Nitric Oxide Synthases 1, 2, and 3 to Airway Hyperresponsiveness and Inflammation in a Murine Model of Asthma

doi:10.1084/jem.189.10.1621

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J. Exp. Med., Volume 189, Number 10, May 17, 1999 1621-1630

Contribution of Nitric Oxide Synthases 1, 2, and 3 to Airway Hyperresponsiveness and Inflammation in a Murine Model of Asthma

By George T. De Sanctis,^* James A. MacLean, Kaoru Hamada,^§ Sanjay Mehta, Jeremy A. Scott, Aiping Jiao,^* Chandri N. Yandava,^* Lester Kobzik,^§ Walter W. Wolyniec,^¶ Attila J. Fabian,^** Changaram S. Venugopal,^* Hartmut Grasemann,^* Paul L. Huang,^** and Jeffrey M. Drazen^*

From the ^* Pulmonary and Critical Care Divisions, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115; the Department of Allergy and Clinical Immunology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114; the ^§ Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; the Pulmonary Division, Departments of Medicine and Pharmacology, London Health Sciences Centre, University of Western Ontario, London, Ontario N6A 4G5, Canada; ^¶ Boehringer Ingelheim Pharmaceuticals, Ridgefield, Connecticut 06877; and the ^** Cardiac Unit and Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114

Asthma is a chronic disease characterized by increased airway responsiveness and airway inflammation. The functional roleof nitric oxide (NO) and the various nitric oxide synthase (NOS)isoforms in human asthma is controversial. To investigate therole of NO in an established model of allergic asthma, mice withtargeted deletions of the three known isoforms of NOS (NOS1, 2,and 3) were studied. Although the inducible (NOS2) isoform wassignificantly upregulated in the lungs of ovalbumin (OVA)-sensitizedand -challenged (OVA/OVA) wild-type (WT) mice and was undetectablein similarly treated NOS2-deficient mice, airway responsivenesswas not significantly different between these groups. OVA/OVAendothelial (NOS3)-deficient mice were significantly more responsiveto methacholine challenge compared with similarly treated NOS1and NOS1&3-deficient mice. Airway responsiveness in OVA/OVA neuronal(NOS1)-deficient and neuronal/endothelial (NOS1&3) double-deficientmice was significantly less than that observed in similarly treatedNOS2 and WT groups. These findings demonstrate an important functionfor the nNOS isoform in controlling the inducibility of airwayhyperresponsiveness in this model of allergicasthma.

Key words: nitric oxide; allergen; mice; asthma; nitric oxide synthase

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