J. Exp. Med., Volume 188, Number 9, November 2, 1998 1657-1668

Characterization of Gene Expression in Resting and Activated Mast Cells

By Huaxian Chen,^* Michael Centola,^* Stephen F. Altschul, and Henry Metzger^*

From the ^* Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases and the  National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20892

To characterize gene expression in activated mast cells more comprehensively than heretofore, we surveyed the changes in genetic transcripts by the method of serial analysis of gene expression in the RBL-2H3 line of rat mast cells before and after they were stimulated through their receptors with high affinity for immunoglobulin E (FcRI). A total of 40,759 transcripts derived from 11,300 genes were analyzed. Among the diverse genes that had not been previously associated with mast cells and that were constitutively expressed were those for the cytokine macrophage migration inhibitory factor neurohormone receptors such as growth hormone- releasing factor and melatonin and components of the exocytotic machinery. In addition, several dozen transcripts were differentially expressed in response to antigen-induced clustering of the FcRI. Included among these were the genes for preprorelaxin, mitogen-activated protein kinase kinase 3, and the dual specificity protein phosphatase, rVH6. Significantly, the majority of genes differentially expressed in this well-studied model of mast cell activation have not been identified before this analysis.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Zhao, Y., Li, Q., Yao, C., Wang, Z., Zhou, Y., Wang, Y., Liu, L., Wang, Y., Wang, L., Qiao, Z. (2006). Characterization and quantification of mRNA transcripts in ejaculated spermatozoa of fertile men by serial analysis of gene expression. Hum Reprod 21: 1583-1590 [Abstract] [Full Text]  
• Hernandez-Hansen, V., Bard, J. D. J., Tarleton, C. A., Wilder, J. A., Lowell, C. A., Wilson, B. S., Oliver, J. M. (2005). Increased Expression of Genes Linked to Fc{epsilon}RI Signaling and to Cytokine and Chemokine Production in Lyn-Deficient Mast Cells. J. Immunol. 175: 7880-7888 [Abstract] [Full Text]  
• Pylouster, J., Senamaud-Beaufort, C., Saison-Behmoaras, T. E. (2005). WEBSAGE: a web tool for visual analysis of differentially expressed human SAGE tags. Nucleic Acids Res 33: W693-W695 [Abstract] [Full Text]  
• Madden, S. L., Cook, B. P., Nacht, M., Weber, W. D., Callahan, M. R., Jiang, Y., Dufault, M. R., Zhang, X., Zhang, W., Walter-Yohrling, J., Rouleau, C., Akmaev, V. R., Wang, C. J., Cao, X., St. Martin, T. B., Roberts, B. L., Teicher, B. A., Klinger, K. W., Stan, R.-V., Lucey, B., Carson-Walter, E. B., Laterra, J., Walter, K. A. (2004). Vascular Gene Expression in Nonneoplastic and Malignant Brain. Am. J. Pathol. 165: 601-608 [Abstract] [Full Text]  
• Izzo, G., d'Istria, M., Serino, I., Minucci, S. (2004). Inhibition of the increased 17{beta}-estradiol-induced mast cell number by melatonin in the testis of the frog Rana esculenta, in vivo and in vitro. J. Exp. Biol. 207: 437-441 [Abstract] [Full Text]  
• Schwartz, F., Duka, A., Triantafyllidi, E., Johns, C., Duka, I., Cui, J., Gavras, H. (2003). Serial analysis of gene expression in mouse kidney following angiotensin II administration. Physiol. Genomics 16: 90-98 [Abstract] [Full Text]  
• Baumruker, T., Csonga, R., Pursch, E., Pfeffer, A., Urtz, N., Sutton, S., Bofill-Cardona, E., Cooke, M., Prieschl, E. (2003). Activation of mast cells by incorporation of cholesterol into rafts. Int Immunol 15: 1207-1218 [Abstract] [Full Text]  
• Zhao, F.-Q., Sheng, Z.-M., Tsai, M. M., Hubbs, A. E., Wang, R., O'Leary, T. J., Izon, D. J., Taubenberger, J. K. (2002). Serial analysis of gene expression in murine fetal thymocyte cell lines. Int Immunol 14: 1383-1395 [Abstract] [Full Text]  
• Uchida, T., Suto, H., Ra, C., Ogawa, H., Kobata, T., Okumura, K. (2002). Preferential expression of Th2-type chemokine and its receptor in atopic dermatitis. Int Immunol 14: 1431-1438 [Abstract] [Full Text]  
• Ruijter, J. M., Van Kampen, A. H. C., Baas, F. (2002). Statistical evaluation of SAGE libraries: consequences for experimental design. Physiol. Genomics 11: 37-44 [Abstract] [Full Text]  
• Zelenika, D., Adams, E., Humm, S., Graca, L., Thompson, S., Cobbold, S. P., Waldmann, H. (2002). Regulatory T Cells Overexpress a Subset of Th2 Gene Transcripts. J. Immunol. 168: 1069-1079 [Abstract] [Full Text]  
• Nakajima, T., Matsumoto, K., Suto, H., Tanaka, K., Ebisawa, M., Tomita, H., Yuki, K., Katsunuma, T., Akasawa, A., Hashida, R., Sugita, Y., Ogawa, H., Ra, C., Saito, H. (2001). Gene expression screening of human mast cells and eosinophils using high-density oligonucleotide probe arrays: abundant expression of major basic protein in mast cells. Blood 98: 1127-1134 [Abstract] [Full Text]  
• Polyak, K., Riggins, G. J. (2001). Gene Discovery Using the Serial Analysis of Gene Expression Technique: Implications for Cancer Research. JCO 19: 2948-2958 [Abstract] [Full Text]  
• LINDSTEDT, K. A., WANG, Y., SHIOTA, N., SAARINEN, J., HYYTIAINEN, M., KOKKONEN, J. O., KESKI-OJA, J., KOVANEN, P. T. (2001). Activation of paracrine TGF-{beta}1 signaling upon stimulation and degranulation of rat serosal mast cells: a novel function for chymase. FASEB J. 15: 1377-1388 [Abstract] [Full Text]  
• Liu, Z.-J., Haleem-Smith, H., Chen, H., Metzger, H. (2001). Unexpected signals in a system subject to kinetic proofreading. Proc. Natl. Acad. Sci. USA 10.1073/pnas.121171998v1 [Abstract] [Full Text]  
• ST-AMAND, J., OKAMURA, K., MATSUMOTO, K., SHIMIZU, S., SOGAWA, Y. (2001). Characterization of control and immobilized skeletal muscle: an overview from genetic engineering. FASEB J. 15: 684-692 [Abstract] [Full Text]  
• Huminiecki, L., Bicknell, R. (2000). In Silico Cloning of Novel Endothelial-Specific Genes. Genome Res 10: 1796-1806 [Abstract] [Full Text]  
• Suzuki, T., Hashimoto, S.-i., Toyoda, N., Nagai, S., Yamazaki, N., Dong, H.-Y., Sakai, J., Yamashita, T., Nukiwa, T., Matsushima, K. (2000). Comprehensive gene expression profile of LPS-stimulated human monocytes by SAGE. Blood 96: 2584-2591 [Abstract] [Full Text]  
• Stollberg, J., Urschitz, J., Urban, Z., Boyd, C. D. (2000). A Quantitative Evaluation of SAGE. Genome Res 10: 1241-1248 [Abstract] [Full Text]  
• Lash, A. E., Tolstoshev, C. M., Wagner, L., Schuler, G. D., Strausberg, R. L., Riggins, G. J., Altschul, S. F. (2000). SAGEmap: A Public Gene Expression Resource. Genome Res 10: 1051-1060 [Abstract] [Full Text]  
• Paumet, F., Le Mao, J., Martin, S., Galli, T., David, B., Blank, U., Roa, M. (2000). Soluble NSF Attachment Protein Receptors (SNAREs) in RBL-2H3 Mast Cells: Functional Role of Syntaxin 4 in Exocytosis and Identification of a Vesicle-Associated Membrane Protein 8-Containing Secretory Compartment. J. Immunol. 164: 5850-5857 [Abstract] [Full Text]  
• Lal, A., Lash, A. E., Altschul, S. F., Velculescu, V., Zhang, L., McLendon, R. E., Marra, M. A., Prange, C., Morin, P. J., Polyak, K., Papadopoulos, N., Vogelstein, B., Kinzler, K. W., Strausberg, R. L., Riggins, G. J. (1999). A Public Database for Gene Expression in Human Cancers. Cancer Res. 59: 5403-5407 [Abstract] [Full Text]  
• Plager, D. A., Loegering, D. A., Weiler, D. A., Checkel, J. L., Wagner, J. M., Clarke, N. J., Naylor, S., Page, S. M., Thomas, L. L., Akerblom, I., Cocks, B., Stuart, S., Gleich, G. J. (1999). A Novel and Highly Divergent Homolog of Human Eosinophil Granule Major Basic Protein. J. Biol. Chem. 274: 14464-14473 [Abstract] [Full Text]  
• Liu, Z.-J., Haleem-Smith, H., Chen, H., Metzger, H. (2001). From the Cover: Unexpected signals in a system subject to kinetic proofreading. Proc. Natl. Acad. Sci. USA 98: 7289-7294 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS