J. Exp. Med., Volume 188, Number 8, October 19, 1998 1529-1534

CD1d-restricted Recognition of Synthetic Glycolipid Antigens by Human Natural Killer T Cells

By Franca M. Spada,^* Yasuhiko Koezuka, and Steven A. Porcelli^*

From the ^* Lymphocyte Biology Section, Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115; and the  Pharmaceutical Research Laboratory, Kirin Brewery, Gunma 370-12, Japan

A conserved subset of mature circulating T cells in humans expresses an invariant V24-JQ T cell receptor (TCR)- chain rearrangement and several natural killer (NK) locus-encoded C-type lectins. These human T cells appear to be precise homologues of the subset of NK1.1⁺ TCR-/⁺ T cells, often referred to as NK T cells, which was initially identified in mice. Here we show that human NK T cell clones are strongly and specifically activated by the same synthetic glycolipid antigens as have been shown recently to stimulate murine NK T cells. Responses of human NK T cells to these synthetic glycolipids, consisting of certain -anomeric sugars conjugated to an acylated phytosphingosine base, required presentation by antigen-presenting cells expressing the major histocompatibility complex class I-like CD1d protein. Presentation of synthetic glycolipid antigens to human NK T cells required internalization of the glycolipids by the antigen-presenting cell and normal endosomal targeting of CD1d. Recognition of these compounds by human NK T cells triggered proliferation, cytokine release, and cytotoxic activity. These results demonstrate a striking parallel in the specificity of NK T cells in humans and mice, thus providing further insight into the potential mechanisms of immune recognition by NK T cells and the immunological function of this unique T cell subset.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Itonori, S., Yamawaki, S., Aoki, K., Yamamoto, K., Hada, N., Takeda, T., Dulaney, J. T., Sugita, M. (2008). Structural characterization of glycosylinositolphospholipids with a blood group type B sugar unit from the edible mushroom, Hypsizygus marmoreus. Glycobiology 18: 540-548 [Abstract] [Full Text]  
• Barral, P., Eckl-Dorna, J., Harwood, N. E., De Santo, C., Salio, M., Illarionov, P., Besra, G. S., Cerundolo, V., Batista, F. D. (2008). B cell receptor-mediated uptake of CD1d-restricted antigen augments antibody responses by recruiting invariant NKT cell help in vivo. Proc. Natl. Acad. Sci. USA 105: 8345-8350 [Abstract] [Full Text]  
• Yamaura, A., Hotta, C., Nakazawa, M., Van Kaer, L., Minami, M. (2008). Human invariant V{alpha}24+ natural killer T cells acquire regulatory functions by interacting with IL-10-treated dendritic cells. Blood 111: 4254-4263 [Abstract] [Full Text]  
• Kim, J. H., Choi, E. Y., Chung, D. H. (2007). Donor Bone Marrow Type II (Non-V{alpha}14J{alpha}18 CD1d-Restricted) NKT Cells Suppress Graft-Versus-Host Disease by Producing IFN-{gamma} and IL-4. J. Immunol. 179: 6579-6587 [Abstract] [Full Text]  
• Adotevi, O., Vingert, B., Freyburger, L., Shrikant, P., Lone, Y.-C., Quintin-Colonna, F., Haicheur, N., Amessou, M., Herbelin, A., Langlade-Demoyen, P., Fridman, W. H., Lemonnier, F., Johannes, L., Tartour, E. (2007). B Subunit of Shiga Toxin-Based Vaccines Synergize with {alpha}-Galactosylceramide to Break Tolerance against Self Antigen and Elicit Antiviral Immunity. J. Immunol. 179: 3371-3379 [Abstract] [Full Text]  
• Coppieters, K., Van Beneden, K., Jacques, P., Dewint, P., Vervloet, A., Vander Cruyssen, B., Van Calenbergh, S., Chen, G., Franck, R. W., Verbruggen, G., Deforce, D., Matthys, P., Tsuji, M., Rottiers, P., Elewaut, D. (2007). A Single Early Activation of Invariant NK T Cells Confers Long-Term Protection against Collagen-Induced Arthritis in a Ligand-Specific Manner. J. Immunol. 179: 2300-2309 [Abstract] [Full Text]  
• Chen, X., Wang, X., Keaton, J. M., Reddington, F., Illarionov, P. A., Besra, G. S., Gumperz, J. E. (2007). Distinct Endosomal Trafficking Requirements for Presentation of Autoantigens and Exogenous Lipids by Human CD1d Molecules. J. Immunol. 178: 6181-6190 [Abstract] [Full Text]  
• Coppieters, K., Dewint, P., Van Beneden, K., Jacques, P., Seeuws, S., Verbruggen, G., Deforce, D., Elewaut, D. (2007). NKT cells: manipulable managers of joint inflammation. Rheumatology (Oxford) 46: 565-571 [Abstract] [Full Text]  
• Molling, J. W., Langius, J. A.E., Langendijk, J. A., Leemans, C. R., Bontkes, H. J., van der Vliet, H. J.J., von Blomberg, B. M. E., Scheper, R. J., van den Eertwegh, A. J.M. (2007). Low Levels of Circulating Invariant Natural Killer T Cells Predict Poor Clinical Outcome in Patients With Head and Neck Squamous Cell Carcinoma. JCO 25: 862-868 [Abstract] [Full Text]  
• Im, J. S., Tapinos, N., Chae, G.-T., Illarionov, P. A., Besra, G. S., DeVries, G. H., Modlin, R. L., Sieling, P. A., Rambukkana, A., Porcelli, S. A. (2006). Expression of CD1d Molecules by Human Schwann Cells and Potential Interactions with Immunoregulatory Invariant NK T Cells. J. Immunol. 177: 5226-5235 [Abstract] [Full Text]  
• Lin, H., Nieda, M., Hutton, J. F., Rozenkov, V., Nicol, A. J. (2006). Comparative gene expression analysis of NKT cell subpopulations. J. Leukoc. Biol. 80: 164-173 [Abstract] [Full Text]  
• Boyson, J. E., Nagarkatti, N., Nizam, L., Exley, M. A., Strominger, J. L. (2006). Gestation stage-dependent mechanisms of invariant natural killer T cell-mediated pregnancy loss. Proc. Natl. Acad. Sci. USA 103: 4580-4585 [Abstract] [Full Text]  
• Kim, H. J., Kim, H. Y., Kim, B. K., Kim, S., Chung, D. H. (2006). Engagement of Glucocorticoid-Induced TNF Receptor Costimulates NKT Cell Activation In Vitro and In Vivo. J. Immunol. 176: 3507-3515 [Abstract] [Full Text]  
• Brigl, M., van den Elzen, P., Chen, X., Meyers, J. H., Wu, D., Wong, C.-H., Reddington, F., Illarianov, P. A., Besra, G. S., Brenner, M. B., Gumperz, J. E. (2006). Conserved and Heterogeneous Lipid Antigen Specificities of CD1d-Restricted NKT Cell Receptors. J. Immunol. 176: 3625-3634 [Abstract] [Full Text]  
• Adley, M A, Assaf, H A, Hussein, M (2005). Expression of CD1d in human scalp skin and hair follicles: hair cycle related alterations. J. Clin. Pathol. 58: 1278-1282 [Abstract] [Full Text]  
• Chen, Y.-T., Kung, J. T. (2005). CD1d-Independent Developmental Acquisition of Prompt IL-4 Gene Inducibility in Thymus CD161(NK1)-CD44lowCD4+CD8- T Cells Is Associated with Complementarity Determining Region 3-Diverse and Biased V{beta}2/V{beta}7/V{beta}8/V{alpha}3.2 T Cell Receptor Usage. J. Immunol. 175: 6537-6550 [Abstract] [Full Text]  
• Kim, J. H., Kim, H. Y., Kim, S., Chung, J.-H., Park, W. S., Chung, D. H. (2005). Natural Killer T (NKT) Cells Attenuate Bleomycin-Induced Pulmonary Fibrosis by Producing Interferon-{gamma}. Am. J. Pathol. 167: 1231-1241 [Abstract] [Full Text]  
• Tachibana, T., Onodera, H., Tsuruyama, T., Mori, A., Nagayama, S., Hiai, H., Imamura, M. (2005). Increased Intratumor V{alpha}24-Positive Natural Killer T Cells: A Prognostic Factor for Primary Colorectal Carcinomas. Clin. Cancer Res. 11: 7322-7327 [Abstract] [Full Text]  
• Osada, T., Morse, M. A., Lyerly, H. K., Clay, T. M. (2005). Ex vivo expanded human CD4+ regulatory NKT cells suppress expansion of tumor antigen-specific CTLs. Int Immunol 17: 1143-1155 [Abstract] [Full Text]  
• Yue, S. C., Shaulov, A., Wang, R., Balk, S. P., Exley, M. A. (2005). CD1d ligation on human monocytes directly signals rapid NF-{kappa}B activation and production of bioactive IL-12. Proc. Natl. Acad. Sci. USA 102: 11811-11816 [Abstract] [Full Text]  
• Agea, E., Russano, A., Bistoni, O., Mannucci, R., Nicoletti, I., Corazzi, L., Postle, A. D., De Libero, G., Porcelli, S. A., Spinozzi, F. (2005). Human CD1-restricted T cell recognition of lipids from pollens. JEM 202: 295-308 [Abstract] [Full Text]  
• Ishikawa, A., Motohashi, S., Ishikawa, E., Fuchida, H., Higashino, K., Otsuji, M., Iizasa, T., Nakayama, T., Taniguchi, M., Fujisawa, T. (2005). A Phase I Study of {alpha}-Galactosylceramide (KRN7000)-Pulsed Dendritic Cells in Patients with Advanced and Recurrent Non-Small Cell Lung Cancer. Clin. Cancer Res. 11: 1910-1917 [Abstract] [Full Text]  
• Novak, J., Beaudoin, L., Griseri, T., Lehuen, A. (2005). Inhibition of T Cell Differentiation into Effectors by NKT Cells Requires Cell Contacts. J. Immunol. 174: 1954-1961 [Abstract] [Full Text]  
• Van Rhijn, I., Young, D. C., Im, J. S., Levery, S. B., Illarionov, P. A., Besra, G. S., Porcelli, S. A., Gumperz, J., Cheng, T.-Y., Moody, D. B. (2004). CD1d-restricted T cell activation by nonlipidic small molecules. Proc. Natl. Acad. Sci. USA 101: 13578-13583 [Abstract] [Full Text]  
• Nieda, M., Okai, M., Tazbirkova, A., Lin, H., Yamaura, A., Ide, K., Abraham, R., Juji, T., Macfarlane, D. J., Nicol, A. J. (2004). Therapeutic activation of V{alpha}24+V{beta}11+ NKT cells in human subjects results in highly coordinated secondary activation of acquired and innate immunity. Blood 103: 383-389 [Abstract] [Full Text]  
• Im, J. S., Yu, K. O. A., Illarionov, P. A., LeClair, K. P., Storey, J. R., Kennedy, M. W., Besra, G. S., Porcelli, S. A. (2004). Direct Measurement of Antigen Binding Properties of CD1 Proteins Using Fluorescent Lipid Probes. J. Biol. Chem. 279: 299-310 [Abstract] [Full Text]  
• Teige, A., Teige, I., Lavasani, S., Bockermann, R., Mondoc, E., Holmdahl, R., Issazadeh-Navikas, S. (2004). CD1-Dependent Regulation of Chronic Central Nervous System Inflammation in Experimental Autoimmune Encephalomyelitis. J. Immunol. 172: 186-194 [Abstract] [Full Text]  
• Rauch, J., Gumperz, J., Robinson, C., Skold, M., Roy, C., Young, D. C., Lafleur, M., Moody, D. B., Brenner, M. B., Costello, C. E., Behar, S. M. (2003). Structural Features of the Acyl Chain Determine Self-phospholipid Antigen Recognition by a CD1d-restricted Invariant NKT (iNKT) Cell. J. Biol. Chem. 278: 47508-47515 [Abstract] [Full Text]  
• Stanic, A. K., Shashidharamurthy, R., Bezbradica, J. S., Matsuki, N., Yoshimura, Y., Miyake, S., Choi, E. Y., Schell, T. D., Van Kaer, L., Tevethia, S. S., Roopenian, D. C., Yamamura, T., Joyce, S. (2003). Another View of T Cell Antigen Recognition: Cooperative Engagement of Glycolipid Antigens by Va14Ja18 Natural TCR. J. Immunol. 171: 4539-4551 [Abstract] [Full Text]  
• Cernadas, M., Sugita, M., van der Wel, N., Cao, X., Gumperz, J. E., Maltsev, S., Besra, G. S., Behar, S. M., Peters, P. J., Brenner, M. B. (2003). Lysosomal Localization of Murine CD1d Mediated by AP-3 Is Necessary for NK T Cell Development. J. Immunol. 171: 4149-4155 [Abstract] [Full Text]  
• Skold, M., Behar, S. M. (2003). Role of CD1d-Restricted NKT Cells in Microbial Immunity. Infect. Immun. 71: 5447-5455 [Full Text]  
• Gansuvd, B., Hubbard, W. J., Hutchings, A., Thomas, F. T., Goodwin, J., Wilson, S. B., Exley, M. A., Thomas, J. M. (2003). Phenotypic and Functional Characterization of Long-Term Cultured Rhesus Macaque Spleen-Derived NKT Cells. J. Immunol. 171: 2904-2911 [Abstract] [Full Text]  
• Thomas, S. Y., Hou, R., Boyson, J. E., Means, T. K., Hess, C., Olson, D. P., Strominger, J. L., Brenner, M. B., Gumperz, J. E., Wilson, S. B., Luster, A. D. (2003). CD1d-Restricted NKT Cells Express a Chemokine Receptor Profile Indicative of Th1-Type Inflammatory Homing Cells. J. Immunol. 171: 2571-2580 [Abstract] [Full Text]  
• Azuma, T., Takahashi, T., Kunisato, A., Kitamura, T., Hirai, H. (2003). Human CD4+ CD25+ Regulatory T Cells Suppress NKT Cell Functions. Cancer Res. 63: 4516-4520 [Abstract] [Full Text]  
• Sun, C.-M., Fiette, L., Tanguy, M., Leclerc, C., Lo-Man, R. (2003). Ontogeny and innate properties of neonatal dendritic cells. Blood 102: 585-591 [Abstract] [Full Text]  
• Ulrichs, T., Moody, D. B., Grant, E., Kaufmann, S. H. E., Porcelli, S. A. (2003). T-Cell Responses to CD1-Presented Lipid Antigens in Humans with Mycobacterium tuberculosis Infection. Infect. Immun. 71: 3076-3087 [Abstract] [Full Text]  
• Gansert, J. L., Kiebler, V., Engele, M., Wittke, F., Rollinghoff, M., Krensky, A. M., Porcelli, S. A., Modlin, R. L., Stenger, S. (2003). Human NKT Cells Express Granulysin and Exhibit Antimycobacterial Activity. J. Immunol. 170: 3154-3161 [Abstract] [Full Text]  
• Capone, M., Cantarella, D., Schumann, J., Naidenko, O. V., Garavaglia, C., Beermann, F., Kronenberg, M., Dellabona, P., MacDonald, H. R., Casorati, G. (2003). Human Invariant V{alpha}24-J{alpha}Q TCR Supports the Development of CD1d-Dependent NK1.1+ and NK1.1- T Cells in Transgenic Mice. J. Immunol. 170: 2390-2398 [Abstract] [Full Text]  
• Stanic, A. K., De Silva, A. D., Park, J.-J., Sriram, V., Ichikawa, S., Hirabyashi, Y., Hayakawa, K., Van Kaer, L., Brutkiewicz, R. R., Joyce, S. (2003). Defective presentation of the CD1d1-restricted natural Va14Ja18 NKT lymphocyte antigen caused by beta -D-glucosylceramide synthase deficiency. Proc. Natl. Acad. Sci. USA 100: 1849-1854 [Abstract] [Full Text]  
• Giaccone, G., Punt, C. J. A., Ando, Y., Ruijter, R., Nishi, N., Peters, M., von Blomberg, B. M. E., Scheper, R. J., van der Vliet, H. J. J., van den Eertwegh, A. J. M., Roelvink, M., Beijnen, J., Zwierzina, H., Pinedo, H. M. (2002). A Phase I Study of the Natural Killer T-Cell Ligand {alpha}-Galactosylceramide (KRN7000) in Patients with Solid Tumors. Clin. Cancer Res. 8: 3702-3709 [Abstract] [Full Text]  
• Kang, S.-J., Cresswell, P. (2002). Calnexin, Calreticulin, and ERp57 Cooperate in Disulfide Bond Formation in Human CD1d Heavy Chain. J. Biol. Chem. 277: 44838-44844 [Abstract] [Full Text]  
• Chackerian, A., Alt, J., Perera, V., Behar, S. M. (2002). Activation of NKT Cells Protects Mice from Tuberculosis. Infect. Immun. 70: 6302-6309 [Abstract] [Full Text]  
• Boyson, J. E., Rybalov, B., Koopman, L. A., Exley, M., Balk, S. P., Racke, F. K., Schatz, F., Masch, R., Wilson, S. B., Strominger, J. L. (2002). CD1d and invariant NKT cells at the human maternal-fetal interface. Proc. Natl. Acad. Sci. USA 99: 13741-13746 [Abstract] [Full Text]  
• Procopio, D. O., Almeida, I. C., Torrecilhas, A. C. T., Cardoso, J. E., Teyton, L., Travassos, L. R., Bendelac, A., Gazzinelli, R. T. (2002). Glycosylphosphatidylinositol-Anchored Mucin-Like Glycoproteins from Trypanosoma cruzi Bind to CD1d but Do Not Elicit Dominant Innate or Adaptive Immune Responses Via the CD1d/NKT Cell Pathway. J. Immunol. 169: 3926-3933 [Abstract] [Full Text]  
• Masterson, A. J., Sombroek, C. C., de Gruijl, T. D., Graus, Y. M. F., van der Vliet, H. J. J., Lougheed, S. M., van den Eertwegh, A. J. M., Pinedo, H. M., Scheper, R. J. (2002). MUTZ-3, a human cell line model for the cytokine-induced differentiation of dendritic cells from CD34+ precursors. Blood 100: 701-703 [Abstract] [Full Text]  
• Yanagisawa, K., Seino, K.-i., Ishikawa, Y., Nozue, M., Todoroki, T., Fukao, K. (2002). Impaired Proliferative Response of V{alpha}24 NKT Cells from Cancer Patients Against {alpha}-Galactosylceramide. J. Immunol. 168: 6494-6499 [Abstract] [Full Text]  
• Duthie, M. S., Kahn, S. J. (2002). Treatment with {alpha}-Galactosylceramide Before Trypanosoma cruzi Infection Provides Protection or Induces Failure to Thrive. J. Immunol. 168: 5778-5785 [Abstract] [Full Text]  
• Takahashi, T., Chiba, S., Nieda, M., Azuma, T., Ishihara, S., Shibata, Y., Juji, T., Hirai, H. (2002). Cutting Edge: Analysis of Human V{alpha}24+CD8+ NK T Cells Activated by {alpha}-Galactosylceramide-Pulsed Monocyte-Derived Dendritic Cells. J. Immunol. 168: 3140-3144 [Abstract] [Full Text]  
• Gonzalez-Aseguinolaza, G., Van Kaer, L., Bergmann, C. C., Wilson, J. M., Schmieg, J., Kronenberg, M., Nakayama, T., Taniguchi, M., Koezuka, Y., Tsuji, M. (2002). Natural Killer T Cell Ligand {alpha}-Galactosylceramide Enhances Protective Immunity Induced by Malaria Vaccines. JEM 195: 617-624 [Abstract] [Full Text]  
• Gumperz, J. E., Miyake, S., Yamamura, T., Brenner, M. B. (2002). Functionally Distinct Subsets of CD1d-restricted Natural Killer T Cells Revealed by CD1d Tetramer Staining. JEM 195: 625-636 [Abstract] [Full Text]  
• van der Vliet, H. J. J., von Blomberg, B. M. E., Hazenberg, M. D., Nishi, N., Otto, S. A., van Benthem, B. H., Prins, M., Claessen, F. A., van den Eertwegh, A. J. M., Giaccone, G., Miedema, F., Scheper, R. J., Pinedo, H. M. (2002). Selective Decrease in Circulating V{alpha}24+V{beta}11+ NKT Cells During HIV Type 1 Infection. J. Immunol. 168: 1490-1495 [Abstract] [Full Text]  
• De Silva, A. D., Park, J.-J., Matsuki, N., Stanic, A. K., Brutkiewicz, R. R., Medof, M. E., Joyce, S. (2002). Lipid Protein Interactions: The Assembly of CD1d1 with Cellular Phospholipids Occurs in the Endoplasmic Reticulum. J. Immunol. 168: 723-733 [Abstract] [Full Text]  
• Jahng, A. W., Maricic, I., Pedersen, B., Burdin, N., Naidenko, O., Kronenberg, M., Koezuka, Y., Kumar, V. (2001). Activation of Natural Killer T Cells Potentiates or Prevents Experimental Autoimmune Encephalomyelitis. JEM 194: 1789-1799 [Abstract] [Full Text]  
• Singh, A. K., Wilson, M. T., Hong, S., Olivares-Villagomez, D., Du, C., Stanic, A. K., Joyce, S., Sriram, S., Koezuka, Y., Van Kaer, L. (2001). Natural Killer T Cell Activation Protects Mice Against Experimental Autoimmune Encephalomyelitis. JEM 194: 1801-1811 [Abstract] [Full Text]  
• Gui, M., Li, J., Wen, L.-J., Hardy, R. R., Hayakawa, K. (2001). TCR{beta} Chain Influences But Does Not Solely Control Autoreactivity of V{alpha}14J281T Cells. J. Immunol. 167: 6239-6246 [Abstract] [Full Text]  
• Ikarashi, Y., Mikami, R., Bendelac, A., Terme, M., Chaput, N., Terada, M., Tursz, T., Angevin, E., Lemonnier, F. A., Wakasugi, H., Zitvogel, L. (2001). Dendritic Cell Maturation Overrules H-2D-mediated Natural Killer T (NKT) Cell Inhibition: Critical Role for B7 in CD1d-dependent NKT Cell Interferon {gamma} Production. JEM 194: 1179-1186 [Abstract] [Full Text]  
• Metelitsa, L. S., Naidenko, O. V., Kant, A., Wu, H.-W., Loza, M. J., Perussia, B., Kronenberg, M., Seeger, R. C. (2001). Human NKT Cells Mediate Antitumor Cytotoxicity Directly by Recognizing Target Cell CD1d with Bound Ligand or Indirectly by Producing IL-2 to Activate NK Cells. J. Immunol. 167: 3114-3122 [Abstract] [Full Text]  
• Wang, B., Geng, Y.-B., Wang, C.-R. (2001). CD1-restricted NK T Cells Protect Nonobese Diabetic Mice from Developing Diabetes. JEM 194: 313-320 [Abstract] [Full Text]  
• Kamada, N., Iijima, H., Kimura, K., Harada, M., Shimizu, E., Motohashi, S.-i., Kawano, T., Shinkai, H., Nakayama, T., Sakai, T., Brossay, L., Kronenberg, M., Taniguchi, M. (2001). Crucial amino acid residues of mouse CD1d for glycolipid ligand presentation to V{{alpha}}14 NKT cells. Int Immunol 13: 853-861 [Abstract] [Full Text]  
• Nieda, M., Nicol, A., Koezuka, Y., Kikuchi, A., Lapteva, N., Tanaka, Y., Tokunaga, K., Suzuki, K., Kayagaki, N., Yagita, H., Hirai, H., Juji, T. (2001). TRAIL expression by activated human CD4+V{alpha}24NKT cells induces in vitro and in vivo apoptosis of human acute myeloid leukemia cells. Blood 97: 2067-2074 [Abstract] [Full Text]  
• Wang, B., Chun, T., Rulifson, I. C., Exley, M., Balk, S. P., Wang, C.-R. (2001). Human CD1d Functions as a Transplantation Antigen and a Restriction Element in Mice. J. Immunol. 166: 3829-3836 [Abstract] [Full Text]  
• Kronenberg, M., Naidenko, O., Koning, F. (2001). Right on target: Novel approaches for the direct visualization of CD1-specific T cell responses. Proc. Natl. Acad. Sci. USA 98: 2950-2952 [Full Text]  
• del C. Salamone, M., Rabinovich, G. A., Mendiguren, A. K., Salamone, G. V., Fainboim, L. (2001). Activation-induced expression of CD1d antigen on mature T cells. J. Leukoc. Biol. 69: 207-214 [Abstract] [Full Text]  
• Hayes, S. M., Knight, K. L. (2001). Group 1 CD1 Genes in Rabbit. J. Immunol. 166: 403-410 [Abstract] [Full Text]  
• Pal, E., Tabira, T., Kawano, T., Taniguchi, M., Miyake, S., Yamamura, T. (2001). Costimulation-Dependent Modulation of Experimental Autoimmune Encephalomyelitis by Ligand Stimulation of V{{alpha}}14 NK T Cells. J. Immunol. 166: 662-668 [Abstract] [Full Text]  
• Yang, O. O., Racke, F. K., Nguyen, P. T., Gausling, R., Severino, M. E., Horton, H. F., Byrne, M. C., Strominger, J. L., Wilson, S. B. (2000). CD1d on Myeloid Dendritic Cells Stimulates Cytokine Secretion from and Cytolytic Activity of V{alpha}24J{alpha}Q T Cells: A Feedback Mechanism for Immune Regulation. J. Immunol. 165: 3756-3762 [Abstract] [Full Text]  
• Bonish, B., Jullien, D., Dutronc, Y., Huang, B. B., Modlin, R., Spada, F. M., Porcelli, S. A., Nickoloff, B. J. (2000). Overexpression of CD1d by Keratinocytes in Psoriasis and CD1d-Dependent IFN-{gamma} Production by NK-T Cells. J. Immunol. 165: 4076-4085 [Abstract] [Full Text]  
• Matsuda, J. L., Naidenko, O. V., Gapin, L., Nakayama, T., Taniguchi, M., Wang, C.-R., Koezuka, Y., Kronenberg, M. (2000). Tracking the Response of Natural Killer T Cells to a Glycolipid Antigen Using CD1d Tetramers. JEM 192: 741-754 [Abstract] [Full Text]  
• Burdin, N., Brossay, L., Degano, M., Iijima, H., Gui, M., Wilson, I. A., Kronenberg, M. (2000). Structural requirements for antigen presentation by mouse CD1. Proc. Natl. Acad. Sci. USA 97: 10156-10161 [Abstract] [Full Text]  
• Fuji, N., Ueda, Y., Fujiwara, H., Itoh, T., Yoshimura, T., Yamagishi, H. (2000). Antitumor Effect of {{alpha}}-Galactosylceramide (KRN7000) on Spontaneous Hepatic Metastases Requires Endogenous Interleukin 12 in the Liver. Clin. Cancer Res. 6: 3380-3387 [Abstract] [Full Text]  
• Ishihara, S., Nieda, M., Kitayama, J., Osada, T., Yabe, T., Kikuchi, A., Koezuka, Y., Porcelli, S. A., Tadokoro, K., Nagawa, H., Juji, T. (2000). {alpha}-Glycosylceramides Enhance the Antitumor Cytotoxicity of Hepatic Lymphocytes Obtained from Cancer Patients by Activating CD3-CD56+ NK Cells In Vitro. J. Immunol. 165: 1659-1664 [Abstract] [Full Text]  
• Gonzalez-Aseguinolaza, G., de Oliveira, C., Tomaska, M., Hong, S., Bruna-Romero, O., Nakayama, T., Taniguchi, M., Bendelac, A., Van Kaer, L., Koezuka, Y., Tsuji, M. (2000). alpha -Galactosylceramide-activated Valpha 14 natural killer T cells mediate protection against murine malaria. Proc. Natl. Acad. Sci. USA 97: 8461-8466 [Abstract] [Full Text]  
• Carbone, E., Terrazzano, G., Melian, A., Zanzi, D., Moretta, L., Porcelli, S., Karre, K., Zappacosta, S. (2000). Inhibition of Human NK Cell-Mediated Killing by CD1 Molecules. J. Immunol. 164: 6130-6137 [Abstract] [Full Text]  
• Molano, A., Park, S.-H., Chiu, Y.-H., Nosseir, S., Bendelac, A., Tsuji, M. (2000). Cutting Edge: The IgG Response to the Circumsporozoite Protein Is MHC Class II-Dependent and CD1d-Independent: Exploring the Role of GPIs in NK T Cell Activation and Antimalarial Responses. J. Immunol. 164: 5005-5009 [Abstract] [Full Text]  
• Takahashi, T., Nieda, M., Koezuka, Y., Nicol, A., Porcelli, S. A., Ishikawa, Y., Tadokoro, K., Hirai, H., Juji, T. (2000). Analysis of Human V{alpha}24+ CD4+ NKT Cells Activated by {alpha}-Glycosylceramide-Pulsed Monocyte-Derived Dendritic Cells. J. Immunol. 164: 4458-4464 [Abstract] [Full Text]  
• Illes, Z., Kondo, T., Newcombe, J., Oka, N., Tabira, T., Yamamura, T. (2000). Differential Expression of NK T Cell V{alpha}24J{alpha}Q Invariant TCR Chain in the Lesions of Multiple Sclerosis and Chronic Inflammatory Demyelinating Polyneuropathy. J. Immunol. 164: 4375-4381 [Abstract] [Full Text]  
• van der Vliet, H. J. J., Nishi, N., de Gruijl, T. D., von Blomberg, B. M. E., van den Eertwegh, A. J. M., Pinedo, H. M., Giaccone, G., Scheper, R. J. (2000). Human natural killer T cells acquire a memory-activated phenotype before birth. Blood 95: 2440-2442 [Abstract] [Full Text]  
• Rodionov, D. G., Nordeng, T. W., Kongsvik, T. L., Bakke, O. (2000). The Cytoplasmic Tail of CD1d Contains Two Overlapping Basolateral Sorting Signals. J. Biol. Chem. 275: 8279-8282 [Abstract] [Full Text]  
• Matsuura, A., Kinebuchi, M., Chen, H.-Z., Katabami, S., Shimizu, T., Hashimoto, Y., Kikuchi, K., Sato, N. (2000). NKT Cells in the Rat: Organ-Specific Distribution of NK T Cells Expressing Distinct V{alpha}14 Chains. J. Immunol. 164: 3140-3148 [Abstract] [Full Text]  
• Shinkai, K., Locksley, R. M. (2000). CD1, Tuberculosis, and the Evolution of Major Histocompatibility Complex Molecules. JEM 191: 907-914 [Full Text]  
• Spada, F. M., Grant, E. P., Peters, P. J., Sugita, M., Melian, A., Leslie, D. S., Lee, H. K., van Donselaar, E., Hanson, D. A., Krensky, A. M., Majdic, O., Porcelli, S. A., Morita, C. T., Brenner, M. B. (2000). Self-Recognition of CD1 by {gamma}/{delta} T Cells: Implications for Innate Immunity. JEM 191: 937-948 [Abstract] [Full Text]  
• Kaneko, Y., Harada, M., Kawano, T., Yamashita, M., Shibata, Y., Gejyo, F., Nakayama, T., Taniguchi, M. (2000). Augmentation of V{alpha}14 NKT Cell-mediated Cytotoxicity by Interleukin 4 in an Autocrine Mechanism Resulting in the Development of Concanavalin A-induced Hepatitis. JEM 191: 105-114 [Abstract] [Full Text]  
• Carnaud, C., Lee, D., Donnars, O., Park, S.-H., Beavis, A., Koezuka, Y., Bendelac, A. (1999). Cutting Edge: Cross-Talk Between Cells of the Innate Immune System: NKT Cells Rapidly Activate NK Cells. J. Immunol. 163: 4647-4650 [Abstract] [Full Text]  
• Naidenko, O. V., Maher, J. K., Ernst, W. A., Sakai, T., Modlin, R. L., Kronenberg, M. (1999). Binding and Antigen Presentation of Ceramide-containing Glycolipids by Soluble Mouse and Human CD1d Molecules. JEM 190: 1069-1080 [Abstract] [Full Text]  
• Falcone, M., Yeung, B., Tucker, L., Rodriguez, E., Sarvetnick, N. (1999). A Defect in Interleukin 12–induced Activation and Interferon {gamma} Secretion of Peripheral Natural Killer T Cells in Nonobese Diabetic Mice Suggests New Pathogenic Mechanisms for Insulin-dependent Diabetes Mellitus. JEM 190: 963-972 [Abstract] [Full Text]  
• Kawano, T., Nakayama, T., Kamada, N., Kaneko, Y., Harada, M., Ogura, N., Akutsu, Y., Motohashi, S., Iizasa, T., Endo, H., Fujisawa, T., Shinkai, H., Taniguchi, M. (1999). Antitumor Cytotoxicity Mediated by Ligand-activated Human V{{alpha}}24 NKT Cells. Cancer Res. 59: 5102-5105 [Abstract] [Full Text]  
• Melian, A., Geng, Y.-J., Sukhova, G. K., Libby, P., Porcelli, S. A. (1999). CD1 Expression in Human Atherosclerosis : A Potential Mechanism for T Cell Activation by Foam Cells. Am. J. Pathol. 155: 775-786 [Abstract] [Full Text]  
• Singh, N., Hong, S., Scherer, D. C., Serizawa, I., Burdin, N., Kronenberg, M., Koezuka, Y., Van Kaer, L. (1999). Cutting Edge: Activation of NK T Cells by CD1d and {alpha}-Galactosylceramide Directs Conventional T Cells to the Acquisition of a Th2 Phenotype. J. Immunol. 163: 2373-2377 [Abstract] [Full Text]  
• Toura, I., Kawano, T., Akutsu, Y., Nakayama, T., Ochiai, T., Taniguchi, M. (1999). Cutting Edge: Inhibition of Experimental Tumor Metastasis by Dendritic Cells Pulsed with {alpha}-Galactosylceramide. J. Immunol. 163: 2387-2391 [Abstract] [Full Text]  
• Doherty, D. G., Norris, S., Madrigal-Estebas, L., McEntee, G., Traynor, O., Hegarty, J. E., O'Farrelly, C. (1999). The Human Liver Contains Multiple Populations of NK Cells, T Cells, and CD3+CD56+ Natural T Cells with Distinct Cytotoxic Activities and Th1, Th2, and Th0 Cytokine Secretion Patterns. J. Immunol. 163: 2314-2321 [Abstract] [Full Text]  
• Han, M., Harrison, L., Kehn, P., Stevenson, K., Currier, J., Robinson, M. A. (1999). Invariant or Highly Conserved TCR {alpha} Are Expressed on Double-Negative (CD3+CD4-CD8-) and CD8+ T Cells. J. Immunol. 163: 301-311 [Abstract] [Full Text]  
• Behar, S. M., Dascher, C. C., Grusby, M. J., Wang, C.-R., Brenner, M. B. (1999). Susceptibility of Mice Deficient in CD1D or TAP1 to Infection with Mycobacterium tuberculosis. JEM 189: 1973-1980 [Abstract] [Full Text]  
• Benlagha, K., Weiss, A., Beavis, A., Teyton, L., Bendelac, A. (1999). In Vivo Identification of Glycolipid Antigen-specific T Cells Using Fluorescent CD1d Tetramers. JEM 191: 1895-1904 [Abstract] [Full Text]  
• Eberl, G., Lees, R., Smiley, S. T., Taniguchi, M., Grusby, M. J., MacDonald, H. R. (1999). Tissue-Specific Segregation of CD1d-Dependent and CD1d-Independent NK T Cells. J. Immunol. 162: 6410-6419 [Abstract] [Full Text]  
• Forestier, C., Moreno, E., Pizarro-Cerda, J., Gorvel, J.-P. (1999). Lysosomal Accumulation and Recycling of Lipopolysaccharide to the Cell Surface of Murine Macrophages, an In Vitro and In Vivo Study. J. Immunol. 162: 6784-6791 [Abstract] [Full Text]  
• Nickoloff, B. J., Wrone-Smith, T., Bonish, B., Porcelli, S. A. (1999). Response of Murine and Normal Human Skin to Injection of Allogeneic Blood-Derived Psoriatic Immunocytes: Detection of T Cells Expressing Receptors Typically Present on Natural Killer Cells, Including CD94, CD158, and CD161. Arch Dermatol 135: 546-552 [Abstract] [Full Text]  
• Apostolou, I., Takahama, Y., Belmant, C., Kawano, T., Huerre, M., Marchal, G., Cui, J., Taniguchi, M., Nakauchi, H., Fournie, J.-J., Kourilsky, P., Gachelin, G. (1999). Murine natural killer cells contribute to the granulomatous reaction caused by mycobacterial cell walls. Proc. Natl. Acad. Sci. USA 96: 5141-5146 [Abstract] [Full Text]  
• Kitamura, H., Iwakabe, K., Yahata, T., Nishimura, S.-i., Ohta, A., Ohmi, Y., Sato, M., Takeda, K., Okumura, K., Van Kaer, L., Kawano, T., Taniguchi, M., Nishimura, T. (1999). The Natural Killer T (NKT) Cell Ligand alpha -Galactosylceramide Demonstrates Its Immunopotentiating Effect by Inducing Interleukin (IL)-12 Production by Dendritic Cells and IL-12 Receptor Expression on NKT Cells. JEM 189: 1121-1128 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS