J. Exp. Med., Volume 188, Number 8, October 19, 1998 1465-1471

Assembly of Productive T Cell Receptor  Variable Region Genes Exhibits Allelic Inclusion

By Barry P. Sleckman, Bernard Khor, Robert Monroe, and Frederick W. Alt

From the Howard Hughes Medical Institute, Children's Hospital and Department of Genetics, Harvard Medical School and The Center for Blood Research, Boston, Massachusetts 02115

The generation of a productive "in-frame" T cell receptor  (TCR ), immunoglobulin (Ig) heavy (H) or Ig light (L) chain variable region gene can result in the cessation of rearrangement of the alternate allele, a process referred to as allelic exclusion. This process ensures that most T cells express a single TCR  chain and most B cells express single IgH and IgL chains. Assembly of TCR  and TCR  chain variable region genes exhibit allelic inclusion and and T cells can express two TCR  or TCR chains, respectively. However, it was not known whether assembly of TCR  variable regions genes is regulated in the context of allelic exclusion. To address this issue, we have analyzed TCR  rearrangements in a panel of mouse splenic T cell hybridomas. We find that, similar to TCR  and  variable region genes, assembly of TCR  variable region genes exhibits properties of allelic inclusion. These findings are discussed in the context of T cell development and regulation of rearrangement of TCR  genes.

T cells;  T cells;  T cell receptor rearrangement;  allelic exclusion;  T cell receptor

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