|
|
|
|
|
|
|
||
J. Exp. Med.,
Volume 188, Number 11, December 7, 1998 2163-2173
By
From the * Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan; the Cells from the bone marrow can present peptides that are derived from tumors, transplants, and
self-tissues. Here we describe how dendritic cells (DCs) process phagocytosed cell fragments onto major histocompatibility complex (MHC) class II products with unusual efficacy. This
was monitored with the Y-Ae monoclonal antibody that is specific for complexes of I-Ab
MHC class II presenting a peptide derived from I-E Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut
06520; the § First Department of Pathology, Kansai Medical University, Osaka 570-0074, Japan;
and the Laboratory of Cellular Physiology and Immunology, The Rockefeller University,
New York 10021
. When immature DCs from I-Ab mice
were cultured for 5-20 h with activated I-E+ B blasts, either necrotic or apoptotic, the DCs
produced the epitope recognized by the Y-Ae monoclonal antibody and stimulated T cells reactive with the same MHC-peptide complex. Antigen transfer was also observed with human
cells, where human histocompatibility leukocyte antigen (HLA)-DR includes the same peptide sequence as mouse I-E. Antigen transfer was preceded by uptake of B cell fragments into
MHC class II-rich compartments. Quantitation of the amount of I-E protein in the B cell fragments revealed that phagocytosed I-E was 1-10 thousand times more efficient in generating MHC-peptide complexes than preprocessed I-E peptide. When we injected different I-E-
bearing cells into C57BL/6 mice to look for a similar phenomenon in vivo, we found that
short-lived migrating DCs could be processed by most of the recipient DCs in the lymph node.
The consequence of antigen transfer from migratory DCs to lymph node DCs is not yet
known, but we suggest that in the steady state, i.e., in the absence of stimuli for DC maturation, this transfer leads to peripheral tolerance of the T cell repertoire to self.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
| TABLE OF CONTENTS |
|
