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J. Exp. Med.,
Volume 187, Number 4, February 16, 1998 479-485
By
From the Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle,
Washington 98109-1024
The crystal structure of the complex between hen egg lysozyme and the Fv fragment of a humanized antilysozyme antibody was determined to 2.7-Å resolution. The structure of the antigen combining site in the complex is nearly identical to that of the complexed form of the parent mouse antibody, D1.3. In contrast, the combining sites of the unliganded mouse and
humanized antilysozymes show moderate conformational differences. This disparity suggests
that a conformational readjustment process linked to antigen binding reverses adverse conformations in the complementarity determining regions that had been introduced by engineering
these segments next to human framework regions in the humanized antibody.
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