J. Exp. Med., Volume 187, Number 12, June 15, 1998 1965-1975

Identification of Podocalyxin-like Protein as a High Endothelial Venule Ligand for L-selectin: Parallels to CD34

By Christopher Sassetti,^* Kirsten Tangemann,^* Mark S. Singer,^* David B. Kershaw,^§ and Steven D. Rosen^*

From the ^* Department of Anatomy and the  Program in Immunology, University of California, San Francisco, San Francisco, California 94143; and the ^§ Department of Pediatrics and the  Department of Communicable Disease, University of Michigan, Ann Arbor, Michigan 48109

The leukocyte adhesion molecule, L-selectin, mediates the recruitment of lymphocytes to secondary lymphoid organs via interactions with specific ligands presented on high endothelial venules (HEV). Although the HEV-derived ligands for L-selectin are still incompletely defined, they share a common sialomucin-like structure which is thought to present clustered oligosaccharides to the lectin domain of L-selectin. Podocalyxin-like protein (PCLP) is a transmembrane sialomucin that is similar in structure to the well-characterized L-selectin ligand CD34. PCLP has been shown previously to be expressed on the foot processes of podocytes in the kidney glomerulus as well as on vascular endothelium at some sites. We have determined that PCLP is present on HEV, where it binds to both recombinant L-selectin and the HEV-specific monoclonal antibody MECA-79. Furthermore, purified HEV-derived PCLP is able to support the tethering and rolling of lymphocytes under physiological flow conditions in vitro. These results suggest a novel function for PCLP as an adhesion molecule and allow the definition of conserved structural features in PCLP and CD34, which may be important for L-selectin ligand function.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Kerr, S. C., Fieger, C. B., Snapp, K. R., Rosen, S. D. (2008). Endoglycan, a Member of the CD34 Family of Sialomucins, Is a Ligand for the Vascular Selectins. J. Immunol. 181: 1480-1490 [Abstract] [Full Text]  
• Choo, A. B., Tan, H. L., Ang, S. N., Fong, W. J., Chin, A., Lo, J., Zheng, L., Hentze, H., Philp, R. J., Oh, S. K.W., Yap, M. (2008). Selection Against Undifferentiated Human Embryonic Stem Cells by a Cytotoxic Antibody Recognizing Podocalyxin-Like Protein-1. Stem Cells 26: 1454-1463 [Abstract] [Full Text]  
• Thibault, S., Tardif, M. R., Gilbert, C., Tremblay, M. J. (2007). Virus-associated host CD62L increases attachment of human immunodeficiency virus type 1 to endothelial cells and enhances trans infection of CD4+ T lymphocytes. J. Gen. Virol. 88: 2568-2573 [Abstract] [Full Text]  
• Schopperle, W. M., DeWolf, W. C. (2007). The TRA-1-60 and TRA-1-81 Human Pluripotent Stem Cell Markers Are Expressed on Podocalyxin in Embryonal Carcinoma. Stem Cells 25: 723-730 [Abstract] [Full Text]  
• Lee, D., Schultz, J. B., Knauf, P. A., King, M. R. (2007). Mechanical Shedding of L-selectin from the Neutrophil Surface during Rolling on Sialyl Lewis x under Flow. J. Biol. Chem. 282: 4812-4820 [Abstract] [Full Text]  
• Steagall, R. J., Rusinol, A. E., Truong, Q. A., Han, Z. (2006). HSPA12B Is Predominantly Expressed in Endothelial Cells and Required for Angiogenesis. Arterioscler. Thromb. Vasc. Bio. 26: 2012-2018 [Abstract] [Full Text]  
• Umemoto, E., Tanaka, T., Kanda, H., Jin, S., Tohya, K., Otani, K., Matsutani, T., Matsumoto, M., Ebisuno, Y., Jang, M. H., Fukuda, M., Hirata, T., Miyasaka, M. (2006). Nepmucin, a novel HEV sialomucin, mediates L-selectin-dependent lymphocyte rolling and promotes lymphocyte adhesion under flow. J. Exp. Med. 203: 1603-1614 [Abstract] [Full Text]  
• Tan, P. C., Furness, S. G.B., Merkens, H., Lin, S., McCoy, M. L., Roskelley, C. D., Kast, J., McNagny, K. M. (2006). Na+/H+ Exchanger Regulatory Factor-1 Is a Hematopoietic Ligand for a Subset of the CD34 Family of Stem Cell Surface Proteins. Stem Cells 24: 1150-1161 [Abstract] [Full Text]  
• Casey, G., Neville, P. J., Liu, X., Plummer, S. J., Cicek, M. S., Krumroy, L. M., Curran, A. P., McGreevy, M. R., Catalona, W. J., Klein, E. A., Witte, J. S. (2006). Podocalyxin variants and risk of prostate cancer and tumor aggressiveness. Hum Mol Genet 15: 735-741 [Abstract] [Full Text]  
• Ben-Chetrit, E., Bergmann, S., Sood, R. (2006). Mechanism of the anti-inflammatory effect of colchicine in rheumatic diseases: a possible new outlook through microarray analysis. Rheumatology (Oxford) 45: 274-282 [Abstract] [Full Text]  
• Yamaguchi, T., Kitaya, K., Daikoku, N., Yasuo, T., Fushiki, S., Honjo, H. (2006). Potential Selectin L Ligands Involved in Selective Recruitment of Peripheral Blood CD16(-) Natural Killer Cells into Human Endometrium. Biol. Reprod. 74: 35-40 [Abstract] [Full Text]  
• Matsumoto, M., Atarashi, K., Umemoto, E., Furukawa, Y., Shigeta, A., Miyasaka, M., Hirata, T. (2005). CD43 Functions as a Ligand for E-Selectin on Activated T Cells. J. Immunol. 175: 8042-8050 [Abstract] [Full Text]  
• Doyonnas, R., Nielsen, J. S., Chelliah, S., Drew, E., Hara, T., Miyajima, A., McNagny, K. M. (2005). Podocalyxin is a CD34-related marker of murine hematopoietic stem cells and embryonic erythroid cells. Blood 105: 4170-4178 [Abstract] [Full Text]  
• Hashizume, H., Horibe, T., Yagi, H., Seo, N., Takigawa, M. (2005). Compartmental Imbalance and Aberrant Immune Function of Blood CD123+ (Plasmacytoid) and CD11c+ (Myeloid) Dendritic Cells in Atopic Dermatitis. J. Immunol. 174: 2396-2403 [Abstract] [Full Text]  
• Gauguet, J.-M., Rosen, S. D., Marth, J. D., von Andrian, U. H. (2004). Core 2 branching {beta}1,6-N-acetylglucosaminyltransferase and high endothelial cell N-acetylglucosamine-6-sulfotransferase exert differential control over B- and T-lymphocyte homing to peripheral lymph nodes. Blood 104: 4104-4112 [Abstract] [Full Text]  
• Stanhope-Baker, P., Kessler, P. M., Li, W., Agarwal, M. L., Williams, B. R. G. (2004). The Wilms Tumor Suppressor-1 Target Gene Podocalyxin Is Transcriptionally Repressed by p53. J. Biol. Chem. 279: 33575-33585 [Abstract] [Full Text]  
• Economou, C. G., Kitsiou, P. V., Tzinia, A. K., Panagopoulou, E., Marinos, E., Kershaw, D. B., Kerjaschki, D., Tsilibary, E. C. (2004). Enhanced podocalyxin expression alters the structure of podocyte basal surface. J. Cell Sci. 117: 3281-3294 [Abstract] [Full Text]  
• Kato, Y., Fujita, N., Kunita, A., Sato, S., Kaneko, M., Osawa, M., Tsuruo, T. (2003). Molecular Identification of Aggrus/T1{alpha} as a Platelet Aggregation-inducing Factor Expressed in Colorectal Tumors. J. Biol. Chem. 278: 51599-51605 [Abstract] [Full Text]  
• van Zante, A., Gauguet, J.-M., Bistrup, A., Tsay, D., von Andrian, U. H., Rosen, S. D. (2003). Lymphocyte-HEV Interactions in Lymph Nodes of a Sulfotransferase-deficient Mouse. J. Exp. Med. 198: 1289-1300 [Abstract] [Full Text]  
• M'Rini, C., Cheng, G., Schweitzer, C., Cavanagh, L. L., Palframan, R. T., Mempel, T. R., Warnock, R. A., Lowe, J. B., Quackenbush, E. J., von Andrian, U. H. (2003). A Novel Endothelial L-Selectin Ligand Activity in Lymph Node Medulla That Is Regulated by {alpha}(1,3)-Fucosyltransferase-IV. J. Exp. Med. 198: 1301-1312 [Abstract] [Full Text]  
• Fieger, C. B., Sassetti, C. M., Rosen, S. D. (2003). Endoglycan, a Member of the CD34 Family, Functions as an L-selectin Ligand through Modification with Tyrosine Sulfation and Sialyl Lewis x. J. Biol. Chem. 278: 27390-27398 [Abstract] [Full Text]  
• Leppanen, A., Yago, T., Otto, V. I., McEver, R. P., Cummings, R. D. (2003). Model Glycosulfopeptides from P-selectin Glycoprotein Ligand-1 Require Tyrosine Sulfation and a Core 2-branched O-Glycan to Bind to L-selectin. J. Biol. Chem. 278: 26391-26400 [Abstract] [Full Text]  
• Delcommenne, M., Kannagi, R., Johnson, P. (2002). TNF-{alpha} increases the carbohydrate sulfation of CD44: induction of 6-sulfo N-acetyl lactosamine on N- and O-linked glycans. Glycobiology 12: 613-622 [Abstract] [Full Text]  
• Tu, L., Poe, J. C., Kadono, T., Venturi, G. M., Bullard, D. C., Tedder, T. F., Steeber, D. A. (2002). A Functional Role for Circulating Mouse L-Selectin in Regulating Leukocyte/Endothelial Cell Interactions In Vivo. J. Immunol. 169: 2034-2043 [Abstract] [Full Text]  
• Renkonen, J., Tynninen, O., Hayry, P., Paavonen, T., Renkonen, R. (2002). Glycosylation Might Provide Endothelial Zip Codes for Organ-Specific Leukocyte Traffic into Inflammatory Sites. Am. J. Pathol. 161: 543-550 [Abstract] [Full Text]  
• Samulowitz, U., Kuhn, A., Brachtendorf, G., Nawroth, R., Braun, A., Bankfalvi, A., Bocker, W., Vestweber, D. (2002). Human Endomucin : Distribution Pattern, Expression on High Endothelial Venules, and Decoration with the MECA-79 Epitope. Am. J. Pathol. 160: 1669-1681 [Abstract] [Full Text]  
• Saito, K., Tanaka, T., Kanda, H., Ebisuno, Y., Izawa, D., Kawamoto, S., Okubo, K., Miyasaka, M. (2002). Gene Expression Profiling of Mucosal Addressin Cell Adhesion Molecule-1+ High Endothelial Venule Cells (HEV) and Identification of a Leucine-Rich HEV Glycoprotein as a HEV Marker. J. Immunol. 168: 1050-1059 [Abstract] [Full Text]  
• Doyonnas, R., Kershaw, D. B., Duhme, C., Merkens, H., Chelliah, S., Graf, T., McNagny, K. M. (2001). Anuria, Omphalocele, and Perinatal Lethality in Mice Lacking the CD34-related Protein Podocalyxin. J. Exp. Med. 194: 13-28 [Abstract] [Full Text]  
• Li, X., Tu, L., Murphy, P. G, Kadono, T., Steeber, D. A, Tedder, T. F. (2001). CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin. J. Leukoc. Biol. 69: 565-574 [Abstract] [Full Text]  
• Evans, E., Leung, A., Hammer, D., Simon, S. (2001). Chemically distinct transition states govern rapid dissociation of single L-selectin bonds under force. Proc. Natl. Acad. Sci. USA 10.1073/pnas.061324998v1 [Abstract] [Full Text]  
• Prosper, F., Verfaillie, C. M. (2001). Regulation of hematopoiesis through adhesion receptors. J. Leukoc. Biol. 69: 307-316 [Abstract] [Full Text]  
• Birner, P., Obermair, A., Schindl, M., Kowalski, H., Breitenecker, G., Oberhuber, G. (2001). Selective Immunohistochemical Staining of Blood and Lymphatic Vessels Reveals Independent Prognostic Influence of Blood and Lymphatic Vessel Invasion in Early-Stage Cervical Cancer. Clin. Cancer Res. 7: 93-97 [Abstract] [Full Text]  
• Sackstein, R., Dimitroff, C. J. (2000). A hematopoietic cell L-selectin ligand that is distinct from PSGL-1 and displays N-glycan-dependent binding activity. Blood 96: 2765-2774 [Abstract] [Full Text]  
• Hemmerich, S., Rosen, S. D. (2000). MINI REVIEW Carbohydrate sulfotransferases in lymphocyte homing. Glycobiology 10: 849-856 [Abstract] [Full Text]  
• Takeda, T., Go, W. Y., Orlando, R. A., Farquhar, M. G. (2000). Expression of Podocalyxin Inhibits Cell-Cell Adhesion and Modifies Junctional Properties in Madin-Darby Canine Kidney Cells. Mol. Biol. Cell 11: 3219-3232 [Abstract] [Full Text]  
• Kahn, J., Mehraban, F., Ingle, G., Xin, X., Bryant, J. E., Vehar, G., Schoenfeld, J., Grimaldi, C. J., Peale, F., Draksharapu, A., Lewin, D. A., Gerritsen, M. E. (2000). Gene Expression Profiling in an in Vitro Model of Angiogenesis. Am. J. Pathol. 156: 1887-1900 [Abstract] [Full Text]  
• Watt, S. M., Butler, L. H., Tavian, M., Buhring, H.-J., Rappold, I., Simmons, P. J., Zannettino, A. C. W., Buck, D., Fuchs, A., Doyonnas, R., Chan, J. Y.-H., Levesque, J.-P., Peault, B., Roxanis, I. (2000). Functionally defined CD164 epitopes are expressed on CD34+ cells throughout ontogeny but display distinct distribution patterns in adult hematopoietic and nonhematopoietic tissues. Blood 95: 3113-3124 [Abstract] [Full Text]  
• Sassetti, C., Van Zante, A., Rosen, S. D. (2000). Identification of Endoglycan, a Member of the CD34/Podocalyxin Family of Sialomucins. J. Biol. Chem. 275: 9001-9010 [Abstract] [Full Text]  
• Tu, L., Murphy, P. G., Li, X., Tedder, T. F. (1999). L-Selectin Ligands Expressed by Human Leukocytes Are HECA-452 Antibody-Defined Carbohydrate Epitopes Preferentially Displayed by P-Selectin Glycoprotein Ligand-1. J. Immunol. 163: 5070-5078 [Abstract] [Full Text]  
• Rosen, S. D. (1999). Endothelial Ligands for L-Selectin : From Lymphocyte Recirculation to Allograft Rejection. Am. J. Pathol. 155: 1013-1020 [Full Text]  
• Toppila, S., Paavonen, T., Nieminen, M. S., Hayry, P., Renkonen, R. (1999). Endothelial L-Selectin Ligands Are Likely to Recruit Lymphocytes into Rejecting Human Heart Transplants. Am. J. Pathol. 155: 1303-1310 [Abstract] [Full Text]  
• Kim, Y. J., Borsig, L., Han, H.-L., Varki, N. M., Varki, A. (1999). Distinct Selectin Ligands on Colon Carcinoma Mucins Can Mediate Pathological Interactions among Platelets, Leukocytes, and Endothelium. Am. J. Pathol. 155: 461-472 [Abstract] [Full Text]  
• Bistrup, A., Bhakta, S., Lee, J. K., Belov, Y. Y., Gunn, M. D., Zuo, F.-R., Huang, C.-C., Kannagi, R., Rosen, S. D., Hemmerich, S. (1999). Sulfotransferases of Two Specificities Function in the Reconstitution of High Endothelial Cell Ligands for L-selectin. J. Cell Biol. 145: 899-910 [Abstract] [Full Text]  
• Kimura, N., Mitsuoka, C., Kanamori, A., Hiraiwa, N., Uchimura, K., Muramatsu, T., Tamatani, T., Kansas, G. S., Kannagi, R. (1999). Reconstitution of functional L-selectin ligands on a cultured human endothelial cell line by cotransfection of alpha 1right-arrow3 fucosyltransferase VII and newly cloned GlcNAcbeta :6-sulfotransferase cDNA. Proc. Natl. Acad. Sci. USA 96: 4530-4535 [Abstract] [Full Text]  
• Kawashima, H., Li, Y.-F., Watanabe, N., Hirose, J., Hirose, M., Miyasaka, M. (1999). Identification and characterization of ligands for L-selectin in the kidney. I. Versican, a large chondroitin sulfate proteoglycan, is a ligand for L-selectin. Int Immunol 11: 393-405 [Abstract] [Full Text]  
• Miettinen, A., Solin, M.-L., Reivinen, J., Juvonen, E., Vaisanen, R., Holthofer, H. (1999). Podocalyxin in Rat Platelets and Megakaryocytes. Am. J. Pathol. 154: 813-822 [Abstract] [Full Text]  
• Breiteneder-Geleff, S., Soleiman, A., Kowalski, H., Horvat, R., Amann, G., Kriehuber, E., Diem, K., Weninger, W., Tschachler, E., Alitalo, K., Kerjaschki, D. (1999). Angiosarcomas Express Mixed Endothelial Phenotypes of Blood and Lymphatic Capillaries : Podoplanin as a Specific Marker for Lymphatic Endothelium. Am. J. Pathol. 154: 385-394 [Abstract] [Full Text]  
• Tangemann, K., Gunn, M. D., Giblin, P., Rosen, S. D. (1998). A High Endothelial Cell-Derived Chemokine Induces Rapid, Efficient, and Subset-Selective Arrest of Rolling T Lymphocytes on a Reconstituted Endothelial Substrate. J. Immunol. 161: 6330-6337 [Abstract] [Full Text]  
• Dwir, O., Kansas, Geoffrey. S., Alon, R. (2000). An Activated L-selectin Mutant with Conserved Equilibrium Binding Properties but Enhanced Ligand Recognition under Shear Flow. J. Biol. Chem. 275: 18682-18691 [Abstract] [Full Text]  
• Xie, X., Rivier, A.-S., Zakrzewicz, A., Bernimoulin, M., Zeng, X.-L., Wessel, H. P., Schapira, M., Spertini, O. (2000). Inhibition of Selectin-mediated Cell Adhesion and Prevention of Acute Inflammation by Nonanticoagulant Sulfated Saccharides. STUDIES WITH CARBOXYL-REDUCED AND SULFATED HEPARIN AND WITH TRESTATIN A SULFATE. J. Biol. Chem. 275: 34818-34825 [Abstract] [Full Text]  
• Bruehl, R. E., Bertozzi, C. R., Rosen, S. D. (2000). Minimal Sulfated Carbohydrates for Recognition by L-selectin and the MECA-79 Antibody. J. Biol. Chem. 275: 32642-32648 [Abstract] [Full Text]  
• Palmeri, D., van Zante, A., Huang, C.-C., Hemmerich, S., Rosen, S. D. (2000). Vascular Endothelial Junction-associated Molecule, a Novel Member of the Immunoglobulin Superfamily, Is Localized to Intercellular Boundaries of Endothelial Cells. J. Biol. Chem. 275: 19139-19145 [Abstract] [Full Text]  
• Kawashima, H., Hirose, M., Hirose, J., Nagakubo, D., Plaas, A. H. K., Miyasaka, M. (2000). Binding of a Large Chondroitin Sulfate/Dermatan Sulfate Proteoglycan, Versican, to L-selectin, P-selectin, and CD44. J. Biol. Chem. 275: 35448-35456 [Abstract] [Full Text]  
• Evans, E., Leung, A., Hammer, D., Simon, S. (2001). Chemically distinct transition states govern rapid dissociation of single L-selectin bonds under force. Proc. Natl. Acad. Sci. USA 98: 3784-3789 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS