J. Exp. Med., Volume 186, Number 12, December 15, 1997 2045-2050

BRIEF DEFINITIVE REPORT:
Membrane Fas Ligand Kills Human Peripheral Blood T Lymphocytes, and Soluble Fas Ligand Blocks the Killing

By Takashi Suda,^* Hideo Hashimoto,^* Masato Tanaka,^§ Takahiro Ochi, and Shigekazu Nagata^*§

From the ^* Osaka Bioscience Institute, Department of Molecular Biology, Suita, Osaka 565, Japan;  Department of Orthopaedic Surgery, and ^§ Department of Genetics, Osaka University Medical School Suita, Osaka 565, Japan

It has been believed that the Fas expressed on human peripheral blood T cells (PBT) is nonfunctional, because these cells are insensitive to agonistic anti-Fas/Apo-1 mAbs that efficiently kill in vitro-activated T cells and many Fas-expressing cell lines. Here, we demonstrate that membrane-bound Fas ligand (FasL) kills both fresh and in vitro-activated PBT, indicating that the Fas expressed on fresh PBT is functional. In contrast, soluble FasL kills only the latter. Naive T cells in umbilical cord blood do not express Fas, but can be induced to express Fas by IFN- or by a combination of IL-2 and anti-CD28 mAb, after which they acquire sensitivity to membrane but not to soluble FasL. Soluble FasL inhibited the killing of fresh PBT by membrane FasL. These results indicate that the shedding of FasL from the membrane is a mechanism for downregulating at least part of its killing activity.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Yolcu, E. S., Gu, X., Lacelle, C., Zhao, H., Bandura-Morgan, L., Askenasy, N., Shirwan, H. (2008). Induction of Tolerance to Cardiac Allografts Using Donor Splenocytes Engineered to Display on Their Surface an Exogenous Fas Ligand Protein. J. Immunol. 181: 931-939 [Abstract] [Full Text]  
• Fountoulakis, S., Vartholomatos, G., Kolaitis, N., Frillingos, S., Philippou, G., Tsatsoulis, A. (2008). Differential expression of Fas system apoptotic molecules in peripheral lymphocytes from patients with Graves' disease and Hashimoto's thyroiditis.. Eur J Endocrinol 158: 853-859 [Abstract] [Full Text]  
• Bremer, E., ten Cate, B., Samplonius, D. F., Mueller, N., Wajant, H., Stel, A. J., Chamuleau, M., van de Loosdrecht, A. A., Stieglmaier, J., Fey, G. H., Helfrich, W. (2008). Superior Activity of Fusion Protein scFvRit:sFasL over Cotreatment with Rituximab and Fas Agonists. Cancer Res. 68: 597-604 [Abstract] [Full Text]  
• Farley, S. M., Purdy, D. E., Ryabinina, O. P., Schneider, P., Magun, B. E., Iordanov, M. S. (2008). Fas Ligand-induced Proinflammatory Transcriptional Responses in Reconstructed Human Epidermis: RECRUITMENT OF THE EPIDERMAL GROWTH FACTOR RECEPTOR AND ACTIVATION OF MAP KINASES. J. Biol. Chem. 283: 919-928 [Abstract] [Full Text]  
• Gregory, M. S., Saff, R. R., Marshak-Rothstein, A., Ksander, B. R. (2007). Control of Ocular Tumor Growth and Metastatic Spread by Soluble and Membrane Fas Ligand. Cancer Res. 67: 11951-11958 [Abstract] [Full Text]  
• Clark, G. J., Rao, M., Ju, X., Hart, D. N. J. (2007). Novel human CD4+ T lymphocyte subpopulations defined by CD300a/c molecule expression. J. Leukoc. Biol. 82: 1126-1135 [Abstract] [Full Text]  
• Chang, S. K., Arendt, B. K., Darce, J. R., Wu, X., Jelinek, D. F. (2006). A role for BLyS in the activation of innate immune cells. Blood 108: 2687-2694 [Abstract] [Full Text]  
• Li, Y., Song, Y.-H., Mohler, J., Delafontaine, P. (2006). ANG II induces apoptosis of human vascular smooth muscle via extrinsic pathway involving inhibition of Akt phosphorylation and increased FasL expression. Am. J. Physiol. Heart Circ. Physiol. 290: H2116-H2123 [Abstract] [Full Text]  
• Nachbur, U., Kassahn, D., Yousefi, S., Legler, D. F., Brunner, T. (2006). Posttranscriptional regulation of Fas (CD95) ligand killing activity by lipid rafts. Blood 107: 2790-2796 [Abstract] [Full Text]  
• Bremer, E., Cate, B. t., Samplonius, D. F., de Leij, L. F. M. H., Helfrich, W. (2006). CD7-restricted activation of Fas-mediated apoptosis: a novel therapeutic approach for acute T-cell leukemia. Blood 107: 2863-2870 [Abstract] [Full Text]  
• Bourcier, C., Jacquel, A., Hess, J., Peyrottes, I., Angel, P., Hofman, P., Auberger, P., Pouyssegur, J., Pages, G. (2006). p44 Mitogen-Activated Protein Kinase (Extracellular Signal-Regulated Kinase 1)-Dependent Signaling Contributes to Epithelial Skin Carcinogenesis.. Cancer Res. 66: 2700-2707 [Abstract] [Full Text]  
• Stone, G. W., Barzee, S., Snarsky, V., Kee, K., Spina, C. A., Yu, X.-F., Kornbluth, R. S. (2006). Multimeric Soluble CD40 Ligand and GITR Ligand as Adjuvants for Human Immunodeficiency Virus DNA Vaccines. J. Virol. 80: 1762-1772 [Abstract] [Full Text]  
• Rabu, C., Quemener, A., Jacques, Y., Echasserieau, K., Vusio, P., Lang, F. (2005). Production of Recombinant Human Trimeric CD137L (4-1BBL): CROSS-LINKING IS ESSENTIAL TO ITS T CELL CO-STIMULATION ACTIVITY. J. Biol. Chem. 280: 41472-41481 [Abstract] [Full Text]  
• Gregory, M. S., Koh, S., Huang, E., Saff, R. R., Marshak-Rothstein, A., Mukai, S., Ksander, B. R. (2005). A Novel Treatment for Ocular Tumors Using Membrane FasL Vesicles to Activate Innate Immunity and Terminate Immune Privilege. IOVS 46: 2495-2502 [Abstract] [Full Text]  
• Dotti, G., Savoldo, B., Pule, M., Straathof, K. C., Biagi, E., Yvon, E., Vigouroux, S., Brenner, M. K., Rooney, C. M. (2005). Human cytotoxic T lymphocytes with reduced sensitivity to Fas-induced apoptosis. Blood 105: 4677-4684 [Abstract] [Full Text]  
• Drynda, A., Quax, P. H. A., Neumann, M., van der Laan, W. H., Pap, G., Drynda, S., Meinecke, I., Kekow, J., Neumann, W., Huizinga, T. W. J., Naumann, M., Konig, W., Pap, T. (2005). Gene Transfer of Tissue Inhibitor of Metalloproteinases-3 Reverses the Inhibitory Effects of TNF-{alpha} on Fas-Induced Apoptosis in Rheumatoid Arthritis Synovial Fibroblasts. J. Immunol. 174: 6524-6531 [Abstract] [Full Text]  
• Henkler, F., Behrle, E., Dennehy, K. M., Wicovsky, A., Peters, N., Warnke, C., Pfizenmaier, K., Wajant, H. (2005). The extracellular domains of FasL and Fas are sufficient for the formation of supramolecular FasL-Fas clusters of high stability. J. Cell Biol. 168: 1087-1098 [Abstract] [Full Text]  
• Monari, C., Pericolini, E., Bistoni, G., Casadevall, A., Kozel, T. R., Vecchiarelli, A. (2005). Cryptococcus neoformans Capsular Glucuronoxylomannan Induces Expression of Fas Ligand in Macrophages. J. Immunol. 174: 3461-3468 [Abstract] [Full Text]  
• Askenasy, N., Yolcu, E. S., Yaniv, I., Shirwan, H. (2005). Induction of tolerance using Fas ligand: a double-edged immunomodulator. Blood 105: 1396-1404 [Abstract] [Full Text]  
• Li, Y., Takemura, G., Kosai, K.-i., Takahashi, T., Okada, H., Miyata, S., Yuge, K., Nagano, S., Esaki, M., Khai, N. C., Goto, K., Mikami, A., Maruyama, R., Minatoguchi, S., Fujiwara, T., Fujiwara, H. (2004). Critical Roles for the Fas/Fas Ligand System in Postinfarction Ventricular Remodeling and Heart Failure. Circ. Res. 95: 627-636 [Abstract] [Full Text]  
• Osawa, H., Maruyama, K., Streilein, J. W. (2004). CD95 Ligand Expression on Corneal Epithelium and Endothelium Influences the Fates of Orthotopic and Heterotopic Corneal Allografts in Mice. IOVS 45: 1908-1915 [Abstract] [Full Text]  
• Proussakova, O. V., Rabaya, N. A., Moshnikova, A. B., Telegina, E. S., Turanov, A., Nanazashvili, M. G., Beletsky, I. P. (2003). Oligomerization of Soluble Fas Antigen Induces Its Cytotoxicity. J. Biol. Chem. 278: 36236-36241 [Abstract] [Full Text]  
• Samel, D., Muller, D., Gerspach, J., Assohou-Luty, C., Sass, G., Tiegs, G., Pfizenmaier, K., Wajant, H. (2003). Generation of a FasL-based Proapoptotic Fusion Protein Devoid of Systemic Toxicity due to Cell-surface Antigen-restricted Activation. J. Biol. Chem. 278: 32077-32082 [Abstract] [Full Text]  
• Fukui, M., Imamura, R., Umemura, M., Kawabe, T., Suda, T. (2003). Pathogen-Associated Molecular Patterns Sensitize Macrophages to Fas Ligand-Induced Apoptosis and IL-1{beta} Release. J. Immunol. 171: 1868-1874 [Abstract] [Full Text]  
• Xu, Y., Szalai, A. J., Zhou, T., Zinn, K. R., Chaudhuri, T. R., Li, X., Koopman, W. J., Kimberly, R. P. (2003). Fc{gamma}Rs Modulate Cytotoxicity of Anti-Fas Antibodies: Implications for Agonistic Antibody-Based Therapeutics. J. Immunol. 171: 562-568 [Abstract] [Full Text]  
• Jaleco, S., Swainson, L., Dardalhon, V., Burjanadze, M., Kinet, S., Taylor, N. (2003). Homeostasis of Naive and Memory CD4+ T Cells: IL-2 and IL-7 Differentially Regulate the Balance Between Proliferation and Fas-Mediated Apoptosis. J. Immunol. 171: 61-68 [Abstract] [Full Text]  
• Park, D. R., Thomsen, A. R., Frevert, C. W., Pham, U., Skerrett, S. J., Kiener, P. A., Liles, W. C. (2003). Fas (CD95) Induces Proinflammatory Cytokine Responses by Human Monocytes and Monocyte-Derived Macrophages. J. Immunol. 170: 6209-6216 [Abstract] [Full Text]  
• Hoves, S., Krause, S. W., Halbritter, D., Zhang, H.-G., Mountz, J. D., Scholmerich, J., Fleck, M. (2003). Mature But Not Immature Fas Ligand (CD95L)-Transduced Human Monocyte-Derived Dendritic Cells Are Protected from Fas-Mediated Apoptosis and Can Be Used as Killer APC. J. Immunol. 170: 5406-5413 [Abstract] [Full Text]  
• Askenasy, N., Yolcu, E. S., Wang, Z., Shirwan, H. (2003). Display of Fas Ligand Protein on Cardiac Vasculature as a Novel Means of Regulating Allograft Rejection. Circulation 107: 1525-1531 [Abstract] [Full Text]  
• Holler, N., Tardivel, A., Kovacsovics-Bankowski, M., Hertig, S., Gaide, O., Martinon, F., Tinel, A., Deperthes, D., Calderara, S., Schulthess, T., Engel, J., Schneider, P., Tschopp, J. (2003). Two Adjacent Trimeric Fas Ligands Are Required for Fas Signaling and Formation of a Death-Inducing Signaling Complex. Mol. Cell. Biol. 23: 1428-1440 [Abstract] [Full Text]  
• Knox, P. G., Milner, A. E., Green, N. K., Eliopoulos, A. G., Young, L. S. (2003). Inhibition of Metalloproteinase Cleavage Enhances the Cytotoxicity of Fas Ligand. J. Immunol. 170: 677-685 [Abstract] [Full Text]  
• Silvestris, F, Grinello, D, Tucci, M, Cafforio, P, Dammacco, F (2003). Enhancement of T cell apoptosis correlates with increased serum levels of soluble Fas (CD95/Apo-I) in active lupus. Lupus 12: 8-14 [Abstract]  
• Xiao, S., Jodo, S., Sung, S.-s. J., Marshak-Rothstein, A., Ju, S.-T. (2002). A Novel Signaling Mechanism for Soluble CD95 Ligand. SYNERGY WITH ANTI-CD95 MONOCLONAL ANTIBODIES FOR APOPTOSIS AND NF-kappa B NUCLEAR TRANSLOCATION. J. Biol. Chem. 277: 50907-50913 [Abstract] [Full Text]  
• Li, J. H., Kluger, M. S., Madge, L. A., Zheng, L., Bothwell, A. L. M., Pober, J. S. (2002). Interferon-{gamma} Augments CD95(APO-1/Fas) and Pro-Caspase-8 Expression and Sensitizes Human Vascular Endothelial Cells to CD95-Mediated Apoptosis. Am. J. Pathol. 161: 1485-1495 [Abstract] [Full Text]  
• Bugajska, U., Georgopoulos, N. T., Southgate, J., Johnson, P. W. M., Graber, P., Gordon, J., Selby, P. J., Trejdosiewicz, L. K. (2002). The Effects of Malignant Transformation on Susceptibility of Human Urothelial Cells to CD40-Mediated Apoptosis. JNCI J Natl Cancer Inst 94: 1381-1395 [Abstract] [Full Text]  
• Gregory, M. S., Repp, A. C., Holhbaum, A. M., Saff, R. R., Marshak-Rothstein, A., Ksander, B. R. (2002). Membrane Fas Ligand Activates Innate Immunity and Terminates Ocular Immune Privilege. J. Immunol. 169: 2727-2735 [Abstract] [Full Text]  
• Aschkenazi, S., Straszewski, S., Verwer, K. M.A., Foellmer, H., Rutherford, T., Mor, G. (2002). Differential Regulation and Function of the Fas/Fas Ligand System in Human Trophoblast Cells. Biol. Reprod. 66: 1853-1861 [Abstract] [Full Text]  
• Song, J., Rutherford, T., Naftolin, F., Brown, S., Mor, G. (2002). Hormonal regulation of apoptosis and the Fas and Fas ligand system in human endometrial cells. Mol Hum Reprod 8: 447-455 [Abstract] [Full Text]  
• Lorenzo, E., Ruiz-Ruiz, C., Quesada, A. J., Hernandez, G., Rodriguez, A., Lopez-Rivas, A., Redondo, J. M. (2002). Doxorubicin Induces Apoptosis and CD95 Gene Expression in Human Primary Endothelial Cells through a p53-dependent Mechanism. J. Biol. Chem. 277: 10883-10892 [Abstract] [Full Text]  
• Krueger, A., Baumann, S., Krammer, P. H., Kirchhoff, S. (2001). FLICE-Inhibitory Proteins: Regulators of Death Receptor-Mediated Apoptosis. Mol. Cell. Biol. 21: 8247-8254 [Full Text]  
• Monleon, I., Martinez-Lorenzo, M. J., Monteagudo, L., Lasierra, P., Taules, M., Iturralde, M., Pineiro, A., Larrad, L., Alava, M. A., Naval, J., Anel, A. (2001). Differential Secretion of Fas Ligand- or APO2 Ligand/TNF-Related Apoptosis-Inducing Ligand-Carrying Microvesicles During Activation-Induced Death of Human T Cells. J. Immunol. 167: 6736-6744 [Abstract] [Full Text]  
• Hohlbaum, A. M., Gregory, M. S., Ju, S.-T., Marshak-Rothstein, A. (2001). Fas Ligand Engagement of Resident Peritoneal Macrophages In Vivo Induces Apoptosis and the Production of Neutrophil Chemotactic Factors. J. Immunol. 167: 6217-6224 [Abstract] [Full Text]  
• Wei, Y., Chen, K., Sharp, G. C., Yagita, H., Braley-Mullen, H. (2001). Expression and Regulation of Fas and Fas Ligand on Thyrocytes and Infiltrating Cells During Induction and Resolution of Granulomatous Experimental Autoimmune Thyroiditis. J. Immunol. 167: 6678-6686 [Abstract] [Full Text]  
• Amant, C., Holm, P., Xu, S.-h., Tritman, N., Kearney, M., Losordo, D. W. (2001). Estrogen Receptor-Mediated, Nitric Oxide-Dependent Modulation of the Immunologic Barrier Function of the Endothelium: Regulation of Fas Ligand Expression by Estradiol. Circulation 104: 2576-2581 [Abstract] [Full Text]  
• Jodo, S., Xiao, S., Hohlbaum, A., Strehlow, D., Marshak-Rothstein, A., Ju, S.-T. (2001). Apoptosis-inducing Membrane Vesicles. A NOVEL AGENT WITH UNIQUE PROPERTIES. J. Biol. Chem. 276: 39938-39944 [Abstract] [Full Text]  
• Wasem, C., Frutschi, C., Arnold, D., Vallan, C., Lin, T., Green, D. R., Mueller, C., Brunner, T. (2001). Accumulation and Activation-Induced Release of Preformed Fas (CD95) Ligand During the Pathogenesis of Experimental Graft-Versus-Host Disease. J. Immunol. 167: 2936-2941 [Abstract] [Full Text]  
• Chen, W., Jin, W., Tian, H., Sicurello, P., Frank, M., Orenstein, J. M., Wahl, S. M. (2001). Requirement for Transforming Growth Factor {beta}1 in Controlling T Cell Apoptosis. J. Exp. Med. 194: 439-454 [Abstract] [Full Text]  
• Cerdan, C., Devilard, E., Xerri, L., Olive, D. (2001). The C-class chemokine lymphotactin costimulates the apoptosis of human CD4+ T cells. Blood 97: 2205-2212 [Abstract] [Full Text]  
• Huang, J.-H., Tykocinski, M. L. (2001). CTLA-4-Fas ligand functions as a trans signal converter protein in bridging antigen-presenting cells and T cells. Int Immunol 13: 529-539 [Abstract] [Full Text]  
• Gruslin, A., Qiu, Q., K. Tsang, B. (2001). X-Linked Inhibitor of Apoptosis Protein Expression and the Regulation of Apoptosis During Human Placental Development. Biol. Reprod. 64: 1264-1272 [Abstract] [Full Text]  
• Wongweragiat, S., Searle, R. F., Bulmer, J. N. (2001). Expression of Fas/Fas Ligand by Decidual Leukocytes in Hydatidiform Mole. Biol. Reprod. 64: 784-789 [Abstract] [Full Text]  
• Thomis, D. C., Marktel, S., Bonini, C., Traversari, C., Gilman, M., Bordignon, C., Clackson, T. (2001). A Fas-based suicide switch in human T cells for the treatment of graft-versus-host disease. Blood 97: 1249-1257 [Abstract] [Full Text]  
• Jeremias, I., Kupatt, C., Martin-Villalba, A., Habazettl, H., Schenkel, J., Boekstegers, P., Debatin, K. M. (2000). Involvement of CD95/Apo1/Fas in Cell Death After Myocardial Ischemia. Circulation 102: 915-920 [Abstract] [Full Text]  
• Kuwano, K., Kawasaki, M., Maeyama, T., Hagimoto, N., Nakamura, N., Shirakawa, K., Hara, N. (2000). Soluble Form of Fas and Fas Ligand in BAL Fluid From Patients With Pulmonary Fibrosis and Bronchiolitis Obliterans Organizing Pneumonia. Chest 118: 451-458 [Abstract] [Full Text]  
• Riccioli, A., Starace, D., D'Alessio, A., Starace, G., Padula, F., De Cesaris, P., Filippini, A., Ziparo, E. (2000). TNF-{alpha} and IFN-{gamma} Regulate Expression and Function of the Fas System in the Seminiferous Epithelium. J. Immunol. 165: 743-749 [Abstract] [Full Text]  
• Fulda, S., Meyer, E., Debatin, K.-M. (2000). Metabolic Inhibitors Sensitize for CD95 (APO-1/Fas)-induced Apoptosis by Down-Regulating Fas-associated Death Domain-like Interleukin 1-Converting Enzyme Inhibitory Protein Expression. Cancer Res. 60: 3947-3956 [Abstract] [Full Text]  
• LORZ, C., ORTIZ, A., JUSTO, P., GONZÁLEZ-CUADRADO, S., DUQUE, N., GÓMEZ-GUERRERO, C., EGIDO, J. (2000). Proapoptotic Fas Ligand Is Expressed by Normal Kidney Tubular Epithelium and Injured Glomeruli. J. Am. Soc. Nephrol. 11: 1266-1277 [Abstract] [Full Text]  
• Bellone, G., Smirne, C., Carbone, A., Mareschi, K., Dughera, L., Farina, E. C., Alabiso, O., Valente, G., Emanuelli, G., Rodeck, U. (2000). Production and Pro-Apoptotic Activity of Soluble CD95 Ligand in Pancreatic Carcinoma. Clin. Cancer Res. 6: 2448-2455 [Abstract] [Full Text]  
• Doedens, J. R., Black, R. A. (2000). Stimulation-induced Down-regulation of Tumor Necrosis Factor-alpha Converting Enzyme. J. Biol. Chem. 275: 14598-14607 [Abstract] [Full Text]  
• Hohlbaum, A. M., Moe, S., Marshak-Rothstein, A. (2000). Opposing Effects of Transmembrane and Soluble Fas Ligand Expression on Inflammation and Tumor Cell Survival. J. Exp. Med. 191: 1209-1220 [Abstract] [Full Text]  
• Kim, S., Kim, K.-A., Hwang, D.-Y., Lee, T. H., Kayagaki, N., Yagita, H., Lee, M.-S. (2000). Inhibition of Autoimmune Diabetes by Fas Ligand: The Paradox Is Solved. J. Immunol. 164: 2931-2936 [Abstract] [Full Text]  
• Mandik-Nayak, L., Nayak, S., Sokol, C., Eaton-Bassiri, A., Madaio, M. P., Caton, A. J., Erikson, J. (2000). The origin of anti-nuclear antibodies in bcl-2 transgenic mice. Int Immunol 12: 353-364 [Abstract] [Full Text]  
• Pinkoski, M. J., Brunner, T., Green, D. R., Lin, T. (2000). Fas and Fas ligand in gut and liver. Am. J. Physiol. Gastrointest. Liver Physiol. 278: G354-G366 [Abstract] [Full Text]  
• Sata, M., Suhara, T., Walsh, K. (2000). Vascular Endothelial Cells and Smooth Muscle Cells Differ in Expression of Fas and Fas Ligand and in Sensitivity to Fas Ligand-Induced Cell Death : Implications for Vascular Disease and Therapy. Arterioscler. Thromb. Vasc. Bio. 20: 309-316 [Abstract] [Full Text]  
• Walsh, K., Isner, J. M. (2000). Apoptosis in inflammatory-fibroproliferative disorders of the vessel wall. Cardiovasc Res 45: 756-765 [Abstract] [Full Text]  
• Hayashida, K., Shimaoka, Y., Ochi, T., Lipsky, P. E (2000). Rheumatoid Arthritis Synovial Stromal Cells Inhibit Apoptosis and Up-Regulate Bcl-xL Expression by B Cells in a CD49/CD29-CD106-Dependent Mechanism. J. Immunol. 164: 1110-1116 [Abstract] [Full Text]  
• Min, W.-P., Gorczynski, R., Huang, X.-Y., Kushida, M., Kim, P., Obataki, M., Lei, J., Suri, R. M., Cattral, M. S. (2000). Dendritic Cells Genetically Engineered to Express Fas Ligand Induce Donor-Specific Hyporesponsiveness and Prolong Allograft Survival. J. Immunol. 164: 161-167 [Abstract] [Full Text]  
• Durieu, I., Amsellem, C., Paulin, C., Chambe, M.-T., Bienvenu, J., Bellon, G., Pacheco, Y (1999). Fas and Fas ligand expression in cystic fibrosis airway epithelium. Thorax 54: 1093-1098 [Abstract] [Full Text]  
• Ayroldi, E., D'Adamio, F., Zollo, O., Agostini, M., Moraca, R., Cannarile, L., Migliorati, G., Delfino, D. V., Riccardi, C. (1999). Cloning and Expression of a Short Fas Ligand: A New Alternatively Spliced Product of the Mouse Fas Ligand Gene. Blood 94: 3456-3467 [Abstract] [Full Text]  
• Buzyn, A., Petit, F., Ostankovitch, M., Figueiredo, S., Varet, B., Guillet, J.-G., Ameisen, J.-C., Estaquier, J. (1999). Membrane-Bound Fas (Apo-1/CD95) Ligand on Leukemic Cells: A Mechanism of Tumor Immune Escape in Leukemia Patients. Blood 94: 3135-3140 [Abstract] [Full Text]  
• Dick, A. D., Siepmann, K., Dees, C., Duncan, L., Broderick, C., Liversidge, J., Forrester, J. V. (1999). Fas-Fas Ligand-Mediated Apoptosis within Aqueous during Idiopathic Acute Anterior Uveitis. IOVS 40: 2258-2267 [Abstract] [Full Text]  
• Coakley, G., Manolios, N., Loughran, T. P. Jr, Panayi, G. S., Lanchbury, J. S. (1999). A Fas promoter polymorphism at position -670 in the enhancer region does not confer susceptibility to Felty's and large granular lymphocyte syndromes. Rheumatology (Oxford) 38: 883-886 [Abstract] [Full Text]  
• Ortiz, A., Lorz, C., Egido, J. (1999). The Fas ligand/Fas system in renal injury. Nephrol Dial Transplant 14: 1831-1834 [Full Text]  
• Jenkins, M., Keir, M., McCune, J. M. (1999). Fas Is Expressed Early in Human Thymocyte Development But Does Not Transmit an Apoptotic Signal. J. Immunol. 163: 1195-1204 [Abstract] [Full Text]  
• Martinez-Lorenzo, M. J., Anel, A., Gamen, S., Monleon, I., Lasierra, P., Larrad, L., Pineiro, A., Alava, M. A., Naval, J. (1999). Activated Human T Cells Release Bioactive Fas Ligand and APO2 Ligand in Microvesicles. J. Immunol. 163: 1274-1281 [Abstract] [Full Text]  
• Arnold, R., Seifert, M., Asadullah, K., Volk, H. D. (1999). Crosstalk Between Keratinocytes and T Lymphocytes via Fas/Fas Ligand Interaction: Modulation by Cytokines. J. Immunol. 162: 7140-7147 [Abstract] [Full Text]  
• Miwa, K., Hashimoto, H., Yatomi, T., Nakamura, N., Nagata, S., Suda, T. (1999). Therapeutic effect of an anti-Fas ligand mAb on lethal graft-versus-host disease. Int Immunol 11: 925-931 [Abstract] [Full Text]  
• Sato, K., Nagayama, H., Takahashi, T. A. (1999). Aberrant CD3- and CD28-Mediated Signaling Events in Cord Blood T Cells Are Associated with Dysfunctional Regulation of Fas Ligand-Mediated Cytotoxicity. J. Immunol. 162: 4464-4471 [Abstract] [Full Text]  
• Moulian, N., Renvoize, C., Desodt, C., Serraf, A., Berrih-Aknin, S. (1999). Functional Fas Expression in Human Thymic Epithelial Cells. Blood 93: 2660-2670 [Abstract] [Full Text]  
• Jayaraman, S., Castro, M., O'Sullivan, M., Bragdon, M. J., Holtzman, M. J. (1999). Resistance to Fas-Mediated T Cell Apoptosis in Asthma. J. Immunol. 162: 1717-1722 [Abstract] [Full Text]  
• Peschon, J. J., Slack, J. L., Reddy, P., Stocking, K. L., Sunnarborg, S. W., Lee, D. C., Russell, W. E., Castner, B. J., Johnson, R. S., Fitzner, J. N., Boyce, R. W., Nelson, N., Kozlosky, C. J., Wolfson, M. F., Rauch, C. T., Cerretti, D. P., Paxton, R. J., March, C. J., Black, R. A. (1998). An Essential Role for Ectodomain Shedding in Mammalian Development. Science 282: 1281-1284 [Abstract] [Full Text]  
• Seino, K.-i., Iwabuchi, K., Kayagaki, N., Miyata, R., Nagaoka, I., Matsuzawa, A., Fukao, K., Yagita, H., Okumura, K. (1998). Cutting Edge: Chemotactic Activity of Soluble Fas Ligand Against Phagocytes. J. Immunol. 161: 4484-4488 [Abstract] [Full Text]  
• Shustov, A., Nguyen, P., Finkelman, F., Elkon, K. B., Via, C. S. (1998). Differential Expression of Fas and Fas Ligand in Acute and Chronic Graft-Versus-Host Disease: Up-Regulation of Fas and Fas Ligand Requires CD8+ T Cell Activation and IFN-{gamma} Production. J. Immunol. 161: 2848-2855 [Abstract] [Full Text]  
• Moulian, N., Bidault, J., Planche, C., Berrih-Aknin, S. (1998). Two Signaling Pathways Can Increase Fas Expression in Human Thymocytes. Blood 92: 1297-1307 [Abstract] [Full Text]  
• Kaplan, D., Sieg, S. (1998). Role of the Fas/Fas Ligand Apoptotic Pathway in Human Immunodeficiency Virus Type 1 Disease. J. Virol. 72: 6279-6282 [Full Text]  
• Wesch, D., Marx, S., Kabelitz, D. (1998). Monocyte-Dependent Death of Freshly Isolated T Lymphocytes: Induction by Phorbolester and Mitogens and Differential Effects of Catalase. J. Immunol. 161: 1248-1256 [Abstract] [Full Text]  
• Yao, P. M., Tabas, I. (2000). Free Cholesterol Loading of Macrophages Induces Apoptosis Involving the Fas Pathway. J. Biol. Chem. 275: 23807-23813 [Abstract] [Full Text]  
• Strehlow, D., Jodo, S., Ju, S.-T. (2000). Retroviral membrane display of apoptotic effector molecules. Proc. Natl. Acad. Sci. USA 97: 4209-4214 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS