J. Exp. Med., Volume 186, Number 12, December 15, 1997 2023-2031

Interferon  Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation

By Harald Neumann, Hannes Schmidt, Elke Wilharm, Lüder Behrens, and Hartmut Wekerle

From the Department of Neuroimmunology, Max-Planck-Institute for Psychiatry, D-82152 Martinsried, Germany

We explored expression and possible function of interferon- (IFN-) in cultured fetal (E15) rat dorsal root ganglion neurons combining whole cell patch-clamp electrophysiology with single cell reverse transcriptase polymerase chain reaction and confocal laser immunocytochemistry. Morphologically, we located IFN- protein in the cytoplasm of the neurons in culture as well as in situ during peri- and postnatal development. Transcripts for classic IFN- and for its receptor were determined in probes of cytoplasm sampled from individual cultured neurons, which had been identified by patch clamp electrophysiology. In addition, the cultured neurons expressed both chains of the IFN- receptor.

Locally produced IFN- acts back on its cellular source. Phosphorylation and nuclear translocation of the IFN-inducible transcriptional factor STAT1 as well as IFN--dependent expression of major histocompatibility complex class I molecules on the neuronal membrane were noted in untreated cultures. However, both processes were substantially blocked in the presence of antibodies neutralizing IFN-. Our findings indicate a role of IFN- in autocrine regulation of sensory neurons.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Chen, K., Iribarren, P., Huang, J., Zhang, L., Gong, W., Cho, E. H., Lockett, S., Dunlop, N. M., Wang, J. M. (2007). Induction of the Formyl Peptide Receptor 2 in Microglia by IFN-{gamma} and Synergy with CD40 Ligand. J. Immunol. 178: 1759-1766 [Abstract] [Full Text]  
• Miyatake, Y., Ikeda, H., Ishizu, A., Baba, T., Ichihashi, T., Suzuki, A., Tomaru, U., Kasahara, M., Yoshiki, T. (2006). Role of Neuronal Interferon-{gamma} in the Development of Myelopathy in Rats Infected with Human T-Cell Leukemia Virus Type 1. Am. J. Pathol. 169: 189-199 [Abstract] [Full Text]  
• Brzozka, K., Finke, S., Conzelmann, K.-K. (2006). Inhibition of Interferon Signaling by Rabies Virus Phosphoprotein P: Activation-Dependent Binding of STAT1 and STAT2. J. Virol. 80: 2675-2683 [Abstract] [Full Text]  
• Obojes, K., Andres, O., Kim, K. S., Daubener, W., Schneider-Schaulies, J. (2005). Indoleamine 2,3-Dioxygenase Mediates Cell Type-Specific Anti-Measles Virus Activity of Gamma Interferon. J. Virol. 79: 7768-7776 [Abstract] [Full Text]  
• Mace, G., Jaume, M., Blanpied, C., Stephan, L., Coudert, J. D., Druet, P., Dietrich, G. (2002). Anti-{micro}-opioid-receptor IgG antibodies are commonly present in serum from healthy blood donors: evidence for a role in apoptotic immune cell death. Blood 100: 3261-3268 [Abstract] [Full Text]  
• Kang, H., Suzuki, Y. (2001). Requirement of Non-T Cells That Produce Gamma Interferon for Prevention of Reactivation of Toxoplasma gondii Infection in the Brain. Infect. Immun. 69: 2920-2927 [Abstract] [Full Text]  
• Jin, Y., Dons, L., Kristensson, K., Rottenberg, M. E. (2001). Neural Route of Cerebral Listeria monocytogenes Murine Infection: Role of Immune Response Mechanisms in Controling Bacterial Neuroinvasion. Infect. Immun. 69: 1093-1100 [Abstract] [Full Text]  
• Abendroth, A., Simmons, A., Efstathiou, S., Pereira, R. A. (2000). Infection with an H2 recombinant herpes simplex virus vector results in expression of MHC class I antigens on the surfaces of human neuroblastoma cells in vitro and mouse sensory neurons in vivo. J. Gen. Virol. 81: 2375-2383 [Abstract] [Full Text]  
• Kohm, A. P., Tang2, Y., Sanders, V. M., Jones3, S. B. (2000). Activation of Antigen-Specific CD4+ Th2 Cells and B Cells In Vivo Increases Norepinephrine Release in the Spleen and Bone Marrow. J. Immunol. 165: 725-733 [Abstract] [Full Text]  
• Jensen, M. B., Hegelund, I. V., Lomholt, N. D., Finsen, B., Owens, T. (2000). IFNgamma Enhances Microglial Reactions to Hippocampal Axonal Degeneration. J. Neurosci. 20: 3612-3621 [Abstract] [Full Text]  
• Pereira, R. A., Simmons, A. (1999). Cell Surface Expression of H2 Antigens on Primary Sensory Neurons in Response to Acute but Not Latent Herpes Simplex Virus Infection In Vivo. J. Virol. 73: 6484-6489 [Abstract] [Full Text]  
• Cantin, E., Tanamachi, B., Openshaw, H., Mann, J., Clarke, K. (1999). Gamma Interferon (IFN-gamma ) Receptor Null-Mutant Mice Are More Susceptible to Herpes Simplex Virus Type 1 Infection than IFN-gamma Ligand Null-Mutant Mice. J. Virol. 73: 5196-5200 [Abstract] [Full Text]  
• Saas, P., Boucraut, J., Quiquerez, A.-L., Schnuriger, V., Perrin, G., Desplat-Jego, S., Bernard, D., Walker, P. R., Dietrich, P.-Y. (1999). CD95 (Fas/Apo-1) as a Receptor Governing Astrocyte Apoptotic or Inflammatory Responses: A Key Role in Brain Inflammation?. J. Immunol. 162: 2326-2333 [Abstract] [Full Text]  
• Bot, A., Bot, S., Bona, C. A. (1998). Protective Role of Gamma Interferon during the Recall Response to Influenza Virus. J. Virol. 72: 6637-6645 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS