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From the * Department of Cellular and Molecular Biology, Immunology Group, S-223 62 Lund,
Sweden; and the Three lines of transgenic mice have been generated which express human CD25 under the
control of the 722-base pair region located immediately 5 Basel Institute for Immunology, CH-4005 Basel, Switzerland
of the precursor (pre)-B cell-specific 
5 gene. All three strains express human CD25 in parallel to endogenous 5 on pre-B
cells, but not on mature B lymphocytes or other blood cell lineages. High expression of human
CD25 on B lineage cells of transgenic mice has allowed the identification of a new
B220+CD19
5+ precursor of the B220+CD19+5+ c-kit+ pre-BI cells. Both types of precursors are clonable on stromal cells in the presence of interleukin-7. The CD19 precursors
have a sizeable part of their immunoglobulin heavy chain gene loci in germline configuration,
while the CD19+ pre-BI cells are predominantly DJH rearranged. The results indicate that random integration of the 722-bp 5 region of the 5 gene into the mouse genome confers tissue
and differentiation stage-specific expression of a transgene.
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