J. Exp. Med.
© The Rockefeller University Press
0022-1007/96/12/2439/06 $2.00
Volume 184 December 1996 2439-2444

BRIEF DEFINITIVE REPORT:
CD8 Increases CD8 Coreceptor Function and Participation in TCR-Ligand Binding

By Valery Renard,^* Pedro Romero, Eric Vivier,^* Bernard Malissen,^* and Immanuel F. Luescher

From the ^* Centre d'Immunologie Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique de Marseille-Luminy, Case 906, 13288 Marseille, Cedex 09, France; and  Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066 Epalinges, Switzerland

To study the role of CD8 in T cell function, we derived a CD8/ (CD8^/) T cell hybridoma of the H-2K^d-restricted N9 cytotoxic T lymphocyte clone specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide PbCS 252-260. This hybridoma was transfected either with CD8 alone or together with CD8. All three hybridomas released interleukin 2 upon incubation with L cells expressing K^d-peptide derivative complexes, though CD8/ cells did so more efficiently than CD8/ and especially CD8^/ cells. More strikingly, only CD8/ cells were able to recognize a weak agonist peptide derivative variant. This recognition was abolished by Fab fragments of the anti-K^d 3 monoclonal antibody SF11.1.1 or substitution of K^d D-227 with K, both conditions known to impair CD8 coreceptor function. T cell receptor (TCR) photoaffinity labeling indicated that TCR-ligand binding on CD8/ cells was ~5- and 20-fold more avid than on CD8/a and CD8^/ cells, respectively. SF1-1.1.1 Fab or K^d mutation D227K reduced the TCR photoaffinity labeling on CD8/ cells to approximately the same low levels observed on CD8^/ cells. These results indicate that CD8/ is a more efficient coreceptor than CD8/, because it more avidly strengthens TCR-ligand binding.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Kroger, C. J., Amoah, S., Alexander-Miller, M. A. (2008). Cutting Edge: Dendritic Cells Prime a High Avidity CTL Response Independent of the Level of Presented Antigen. J. Immunol. 180: 5784-5788 [Abstract] [Full Text]  
• Pang, D. J., Hayday, A. C., Bijlmakers, M.-J. (2007). CD8 Raft Localization Is Induced by Its Assembly into CD8{alpha}beta Heterodimers, Not CD8{alpha}{alpha} Homodimers. J. Biol. Chem. 282: 13884-13894 [Abstract] [Full Text]  
• Chang, H.-C., Tan, K., Hsu, Y.-M. (2006). CD8{alpha}beta Has Two Distinct Binding Modes of Interaction with Peptide-Major Histocompatibility Complex Class I. J. Biol. Chem. 281: 28090-28096 [Abstract] [Full Text]  
• Kessels, H. W. H. G., Schepers, K., van den Boom, M. D., Topham, D. J., Schumacher, T. N. M. (2006). Generation of T Cell Help through a MHC Class I-Restricted TCR. J. Immunol. 177: 976-982 [Abstract] [Full Text]  
• Kao, C., Sandau, M. M., Daniels, M. A., Jameson, S. C. (2006). The Sialyltransferase ST3Gal-I Is Not Required for Regulation of CD8-Class I MHC Binding during T Cell Development.. J. Immunol. 176: 7421-7430 [Abstract] [Full Text]  
• Koneru, M., Schaer, D., Monu, N., Ayala, A., Frey, A. B. (2005). Defective Proximal TCR Signaling Inhibits CD8+ Tumor-Infiltrating Lymphocyte Lytic Function. J. Immunol. 174: 1830-1840 [Abstract] [Full Text]  
• Oh, S., Perera, L. P., Burke, D. S., Waldmann, T. A., Berzofsky, J. A. (2004). IL-15/IL-15R{alpha}-mediated avidity maturation of memory CD8+ T cells. Proc. Natl. Acad. Sci. USA 101: 15154-15159 [Abstract] [Full Text]  
• Wong, J. S., Wang, X., Witte, T., Nie, L., Carvou, N., Kern, P., Chang, H.-C. (2003). Stalk Region of {beta}-Chain Enhances the Coreceptor Function of CD8. J. Immunol. 171: 867-874 [Abstract] [Full Text]  
• Doucey, M.-A., Legler, D. F., Faroudi, M., Boucheron, N., Baumgaertner, P., Naeher, D., Cebecauer, M., Hudrisier, D., Ruegg, C., Palmer, E., Valitutti, S., Bron, C., Luescher, I. F. (2003). The {beta}1 and {beta}3 Integrins Promote T Cell Receptor-mediated Cytotoxic T Lymphocyte Activation. J. Biol. Chem. 278: 26983-26991 [Abstract] [Full Text]  
• Hutchinson, S. L., Wooldridge, L., Tafuro, S., Laugel, B., Glick, M., Boulter, J. M., Jakobsen, B. K., Price, D. A., Sewell, A. K. (2003). The CD8 T Cell Coreceptor Exhibits Disproportionate Biological Activity at Extremely Low Binding Affinities. J. Biol. Chem. 278: 24285-24293 [Abstract] [Full Text]  
• Konno, A., Okada, K., Mizuno, K., Nishida, M., Nagaoki, S., Toma, T., Uehara, T., Ohta, K., Kasahara, Y., Seki, H., Yachie, A., Koizumi, S. (2002). CD8alpha alpha memory effector T cells descend directly from clonally expanded CD8alpha +beta high TCRalpha beta T cells in vivo. Blood 100: 4090-4097 [Abstract] [Full Text]  
• Arcaro, A., Gregoire, C., Bakker, T. R., Baldi, L., Jordan, M., Goffin, L., Boucheron, N., Wurm, F., van der Merwe, P. A., Malissen, B., Luescher, I. F. (2001). CD8{beta} Endows CD8 with Efficient Coreceptor Function by Coupling T Cell Receptor/CD3 to Raft-associated CD8/p56lck Complexes. J. Exp. Med. 194: 1485-1495 [Abstract] [Full Text]  
• Cawthon, A. G., Lu, H., Alexander-Miller, M. A. (2001). Peptide Requirement for CTL Activation Reflects the Sensitivity to CD3 Engagement: Correlation with CD8{alpha}{beta} Versus CD8{alpha}{alpha} Expression. J. Immunol. 167: 2577-2584 [Abstract] [Full Text]  
• Arcaro, A., Gregoire, C., Boucheron, N., Stotz, S., Palmer, E., Malissen, B., Luescher, I. F. (2000). Essential Role of CD8 Palmitoylation in CD8 Coreceptor Function. J. Immunol. 165: 2068-2076 [Abstract] [Full Text]  
• Lin, T.-J., Hirji, N., Stenton, G. R., Gilchrist, M., Grill, B. J., Schreiber, A. D., Befus, A. D. (2000). Activation of Macrophage CD8: Pharmacological Studies of TNF and IL-1{beta} Production. J. Immunol. 164: 1783-1792 [Abstract] [Full Text]  
• Daniels, M. A., Jameson, S. C. (2000). Critical Role for CD8 in T Cell Receptor Binding and Activation by Peptide/Major Histocompatibility Complex Multimers. J. Exp. Med. 191: 335-346 [Abstract] [Full Text]  
• Auphan, N., Ghosh, S., Flavell, R. A., Schmitt-Verhulst, A.-M. (1999). Differential Requirements for NF-{kappa}B and AP-1 trans-Activation in Response to Minimal TCR Engagement by a Partial Agonist in Naive CD8 T Cells. J. Immunol. 163: 5219-5227 [Abstract] [Full Text]  
• Kern, P., Hussey, R. E., Spoerl, R., Reinherz, E. L., Chang, H.-C. (1999). Expression, Purification, and Functional Analysis of Murine Ectodomain Fragments of CD8alpha alpha and CD8alpha beta Dimers. J. Biol. Chem. 274: 27237-27243 [Abstract] [Full Text]  
• Stotz, S. H., Bolliger, L., Carbone, F. R., Palmer, E. (1999). T Cell Receptor (TCR) Antagonism without a Negative Signal: Evidence from T Cell Hybridomas Expressing Two Independent TCRs. J. Exp. Med. 189: 253-264 [Abstract] [Full Text]  
• Cruz, D., Sydora, B. C., Hetzel, K., Yakoub, G., Kronenberg, M., Cheroutre, H. (1998). An Opposite Pattern of Selection of a Single T Cell Antigen Receptor in the Thymus and among Intraepithelial Lymphocytes. J. Exp. Med. 188: 255-265 [Abstract] [Full Text]  
• Irie, H. Y., Mong, M. S., Itano, A., Crooks, M. E. C., Littman, D. R., Burakoff, S. J., Robey, E. (1998). The Cytoplasmic Domain of CD8{beta} Regulates Lck Kinase Activation and CD8 T Cell Development. J. Immunol. 161: 183-191 [Abstract] [Full Text]  
• Auphan, N., Simon, A. K., Asnagli, H., Phillips, R. J., Rincon, M., Ghosh, S., Flavell, R. A., Schmitt-Verhulst, A.-M. (1998). Consequences of Intrathymic TCR Engagement by Partial Agonist on Selection Events and Peripheral T Cell Activation Program. J. Immunol. 160: 4810-4821 [Abstract] [Full Text]  
• Wheeler, C. J., Chen, J.-Y., Potter, T. A., Parnes, J. R. (1998). Mechanisms of CD8{beta}-Mediated T Cell Response Enhancement: Interaction with MHC Class I/{beta}2-Microglobulin and Functional Coupling to TCR/CD3. J. Immunol. 160: 4199-4207 [Abstract] [Full Text]  
• Viola, A., Salio, M., Tuosto, L., Linkert, S., Acuto, O., Lanzavecchia, A. (1997). Quantitative Contribution of CD4 and CD8 to T Cell Antigen Receptor Serial Triggering. J. Exp. Med. 186: 1775-1779 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS