J. Exp. Med.
© The Rockefeller University Press
0022-1007/96/12/2085/06 $2.00
Volume 184 December 1996 2085-2090

Heterogeneity Among Ly-49C Natural Killer (NK) Cells: Characterization of Highly Related Receptors with Differing Functions and Expression Patterns

By Jack Brennan,^* Suzanne Lemieux,^§ J. Douglas Freeman,^* Dixie L. Mager,^* and Fumio Takei^*

From the ^* Terry Fox Laboratory, British Columbia Cancer Agency; the  Department of Medical Genetics, and the  Department of Pathology and Laboratory Medicine, the University of British Columbia, Vancouver, British Columbia; and the ^§ Centre de Recherche en Immunologie, Institut Armand-Frappier, Université du Québec, Laval, Québec, Canada

Ly-49C is a member of the polymorphic family of murine NK cell inhibitory receptors. The 5E6 antibody that defines a subset of NK cells responsible for the rejection of parental H-2^d bone marrow by F₁ mice has been shown previously to react with Ly-49C. Here, the 5E6 antibody was found to detect two Ly-49C-related molecules in B6 mice. Two cDNA clones were isolated from B6 NK cells, one identical to previously reported Ly-49C^B6 and the other a novel cDNA. The deduced amino acid sequence of the latter differs from that of Ly-49C^BALB at only 4 residues, whereas the previously reported Ly-49C^B6 differs at 22 residues. Flow cytometric analyses of COS cells transfected with the two cDNAs showed that the 5E6 antibody binds to both Ly-49 molecules, while another anti-Ly-49C antibody, 4LO3311, binds to the newly described Ly-49C but not the previously reported Ly-49C^B6. Two-color flow cytometric analysis detected 5E6⁺4LO3311 as well as 5E6⁺4LO3311⁺ subsets of NK cells from B6, but not BALB/c, mice. The level of Ly-49C expression on B6 NK cells detected by the 4LO3311 antibody was substantially lower than that on BALB/c NK cells. Binding specificity of the novel Ly-49C^B6 was indistinguishable from that of Ly-49C^BALB, whereas no binding was detectable with previously reported Ly-49C^B6. These results demonstrate that the newly described Ly-49C^B6, not the previously reported Ly-49C^B6, is the probable B6 allelic form of Ly49C. The previously reported Ly-49C^B6 must be encoded by a separate gene and should be renamed Ly-49I. The implication of these results with respect to the role of Ly-49C in hybrid resistance is discussed.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• MacFarlane, A. W. IV, Yamazaki, T., Fang, M., Sigal, L. J., Kurosaki, T., Campbell, K. S. (2008). Enhanced NK-cell development and function in BCAP-deficient mice. Blood 112: 131-140 [Abstract] [Full Text]  
• Iizuka, K., Scalzo, A. A., Xian, H., Yokoyama, W. M. (2008). Regulation of the NK Cell Alloreactivity to Bone Marrow Cells by the Combination of the Host NK Gene Complex and MHC Haplotypes. J. Immunol. 180: 3260-3267 [Abstract] [Full Text]  
• Benoit, L. A., Tan, R. (2007). Xenogeneic beta2-Microglobulin Substitution Alters NK Cell Function. J. Immunol. 179: 1466-1474 [Abstract] [Full Text]  
• Rouhi, A., Gagnier, L., Takei, F., Mager, D. L. (2006). Evidence for epigenetic maintenance of Ly49A monoallelic gene expression.. J. Immunol. 176: 2991-2999 [Abstract] [Full Text]  
• Benoit, L. A., Shannon, J., Chamberlain, J. W., Miller, R. G. (2005). Influence of Xenogeneic {beta}2-Microglobulin on Functional Recognition of H-2Kb by the NK Cell Inhibitory Receptor Ly49C. J. Immunol. 175: 3542-3553 [Abstract] [Full Text]  
• Toyama-Sorimachi, N., Tsujimura, Y., Maruya, M., Onoda, A., Kubota, T., Koyasu, S., Inaba, K., Karasuyama, H. (2004). Ly49Q, a member of the Ly49 family that is selectively expressed on myeloid lineage cells and involved in regulation of cytoskeletal architecture. Proc. Natl. Acad. Sci. USA 101: 1016-1021 [Abstract] [Full Text]  
• Florido, M., Correia-Neves, M., Cooper, A. M., Appelberg, R. (2003). The cytolytic activity of natural killer cells is not involved in the restriction of Mycobacterium avium growth. Int Immunol 15: 895-901 [Abstract] [Full Text]  
• Silver, E. T., Lavender, K. J., Gong, D.-E., Hazes, B., Kane, K. P. (2002). Allelic Variation in the Ectodomain of the Inhibitory Ly-49G2 Receptor Alters Its Specificity for Allogeneic and Xenogeneic Ligands. J. Immunol. 169: 4752-4760 [Abstract] [Full Text]  
• Morris, M. A., Liu, J., Arora, V., George, T. C., Klem, J., Schatzle, J. D., Kumar, V., Bennett, M. (2002). B6 Strain Ly49I Inhibitory Gene Expression on T Cells in FVB.Ly49IB6 Transgenic Mice Fails to Prevent Normal T Cell Functions. J. Immunol. 169: 3661-3666 [Abstract] [Full Text]  
• Korten, S., Volkmann, L., Saeftel, M., Fischer, K., Taniguchi, M., Fleischer, B., Hoerauf, A. (2002). Expansion of NK Cells with Reduction of Their Inhibitory Ly-49A, Ly-49C, and Ly-49G2 Receptor-Expressing Subsets in a Murine Helminth Infection: Contribution to Parasite Control. J. Immunol. 168: 5199-5206 [Abstract] [Full Text]  
• Sundback, J., Achour, A., Michaelsson, J., Lindstrom, H., Karre, K. (2002). NK Cell Inhibitory Receptor Ly-49C Residues Involved in MHC Class I Binding. J. Immunol. 168: 793-800 [Abstract] [Full Text]  
• Maeda, M., Lohwasser, S., Yamamura, T., Takei, F. (2001). Regulation of NKT Cells by Ly49: Analysis of Primary NKT Cells and Generation of NKT Cell Line. J. Immunol. 167: 4180-4186 [Abstract] [Full Text]  
• Daniels, K. A., Devora, G., Lai, W. C., O'Donnell, C. L., Bennett, M., Welsh, R. M. (2001). Murine Cytomegalovirus Is Regulated by a Discrete Subset of Natural Killer Cells Reactive with Monoclonal Antibody to Ly49H. J. Exp. Med. 194: 29-44 [Abstract] [Full Text]  
• Fahlen, L., Lendahl, U., Sentman, C. L. (2001). MHC Class I-Ly49 Interactions Shape the Ly49 Repertoire on Murine NK Cells. J. Immunol. 166: 6585-6592 [Abstract] [Full Text]  
• Makrigiannis, A. P., Pau, A. T., Saleh, A., Winkler-Pickett, R., Ortaldo, J. R., Anderson, S. K. (2001). Class I MHC-Binding Characteristics of the 129/J Ly49 Repertoire. J. Immunol. 166: 5034-5043 [Abstract] [Full Text]  
• Van Beneden, K., Stevenaert, F., De Creus, A., Debacker, V., De Boever, J., Plum, J., Leclercq, G. (2001). Expression of Ly49E and CD94/NKG2 on Fetal and Adult NK Cells. J. Immunol. 166: 4302-4311 [Abstract] [Full Text]  
• Imboden, M., Murphy, K. R., Rakhmilevich, A. L., Neal, Z. C., Xiang, R., Reisfeld, R. A., Gillies, S. D., Sondel, P. M. (2001). The Level of MHC Class I Expression on Murine Adenocarcinoma Can Change the Antitumor Effector Mechanism of Immunocytokine Therapy. Cancer Res. 61: 1500-1507 [Abstract] [Full Text]  
• Hui Jia, S., Kurepa, Z., Bai, A., Forman, J. (2000). Comparative Ability of Qdm/Qa-1b, Kb, and Db to Protect Class Ilow Cells from NK-Mediated Lysis In Vivo. J. Immunol. 165: 6142-6147 [Abstract] [Full Text]  
• Liu, J., Morris, M. A., Nguyen, P., George, T. C., Koulich, E., Lai, W. C., Schatzle, J. D., Kumar, V., Bennett, M. (2000). Ly49I NK Cell Receptor Transgene Inhibition of Rejection of H2b Mouse Bone Marrow Transplants. J. Immunol. 164: 1793-1799 [Abstract] [Full Text]  
• Mason, L. H., Willette-Brown, J., Mason, A. T., McVicar, D., Ortaldo, J. R. (2000). Interaction of Ly-49D+ NK Cells with H-2Dd Target Cells Leads to Dap-12 Phosphorylation and IFN-{gamma} Secretion. J. Immunol. 164: 603-611 [Abstract] [Full Text]  
• Kubota, A., Lian, R. H., Lohwasser, S., Salcedo, M., Takei, F. (1999). IFN-{gamma} Production and Cytotoxicity of IL-2-Activated Murine NK Cells Are Differentially Regulated by MHC Class I Molecules. J. Immunol. 163: 6488-6493 [Abstract] [Full Text]  
• Korten, S., Wilk, E., Gessner, J. E., Meyer, D., Schmidt, R. E. (1999). Altered Donor and Recipient Ly49+ NK Cell Subsets in Allogeneic H-2d -> H-2b and H-2b -> H-2d Bone Marrow Chimeras. J. Immunol. 163: 5896-5905 [Abstract] [Full Text]  
• Su, R.-C., Kung, S. K.-P., Silver, E. T., Lemieux, S., Kane, K. P., Miller, R. G. (1999). Ly-49CB6 NK Inhibitory Receptor Recognizes Peptide-Receptive H-2Kb 1. J. Immunol. 163: 5319-5330 [Abstract] [Full Text]  
• Depatie, C., Chalifour, A., Pare, C., Lee, S.-H., Vidal, S. M., Lemieux3, S. (1999). Assessment of Cmv1 candidates by genetic mapping and in vivo antibody depletion of NK cell subsets. Int Immunol 11: 1541-1551 [Abstract] [Full Text]  
• Manilay, J. O., Waneck, G. L., Sykes, M. (1999). Levels of Ly-49 Receptor Expression Are Determined by the Frequency of Interactions with MHC Ligands: Evidence Against Receptor Calibration to a ""Useful"" Level. J. Immunol. 163: 2628-2633 [Abstract] [Full Text]  
• Williams, N. S., Klem, J., Puzanov, I. J., Sivakumar, P. V., Bennett, M., Kumar, V. (1999). Differentiation of NK1.1+, Ly49+ NK Cells from flt3+ Multipotent Marrow Progenitor Cells. J. Immunol. 163: 2648-2656 [Abstract] [Full Text]  
• George, T. C., Ortaldo, J. R., Lemieux, S., Kumar, V., Bennett, M. (1999). Tolerance and Alloreactivity of the Ly49D Subset of Murine NK Cells. J. Immunol. 163: 1859-1867 [Abstract] [Full Text]  
• Lian, R. H., Li, Y., Kubota, S., Mager, D. L., Takei, F. (1999). Recognition of Class I MHC by NK Receptor Ly-49C: Identification of Critical Residues. J. Immunol. 162: 7271-7276 [Abstract] [Full Text]  
• Idris, A. H., Smith, H. R. C., Mason, L. H., Ortaldo, J. R., Scalzo, A. A., Yokoyama, W. M. (1999). The natural killer gene complex genetic locus Chok encodes Ly-49D, a target recognition receptor that activates natural killing. Proc. Natl. Acad. Sci. USA 96: 6330-6335 [Abstract] [Full Text]  
• Kung, S. K. P., Su, R.-C., Shannon, J., Miller, R. G. (1999). The NKR-P1B Gene Product Is an Inhibitory Receptor on SJL/J NK Cells. J. Immunol. 162: 5876-5887 [Abstract] [Full Text]  
• Vos, Q., Snapper, C. M., Mond, J. J. (1999). Heterogeneity in the ability of cytotoxic murine NK cell clones to enhance Ig secretion in vitro. Int Immunol 11: 159-168 [Abstract] [Full Text]  
• Kase, A., Johansson, M. H., Olsson-Alheim, M. Y., Karre, K., Hoglund, P. (1998). External and Internal Calibration of the MHC Class I-Specific Receptor Ly49A on Murine Natural Killer Cells. J. Immunol. 161: 6133-6138 [Abstract] [Full Text]  
• Lian, R. H., Freeman, J. D., Mager, D. L., Takei, F. (1998). Role of Conserved Glycosylation Site Unique to Murine Class I MHC in Recognition by Ly-49 NK Cell Receptor. J. Immunol. 161: 2301-2306 [Abstract] [Full Text]  
• Dorfman, J. R., Raulet, D. H. (1998). Acquisition of Ly49 Receptor Expression by Developing Natural Killer Cells. J. Exp. Med. 187: 609-618 [Abstract] [Full Text]  
• Ortaldo, J. R., Winkler-Pickett, R., Mason, A. T., Mason, L. H. (1998). The Ly-49 Family: Regulation of Cytotoxicity and Cytokine Production in Murine CD3+ Cells. J. Immunol. 160: 1158-1165 [Abstract] [Full Text]  
• Raziuddin, A., Longo, D. L., Mason, L., Ortaldo, J. R., Bennett, M., Murphy, W. J. (1998). Differential Effects of the Rejection of Bone Marrow Allografts by the Depletion of Activating Versus Inhibiting Ly-49 Natural Killer Cell Subsets. J. Immunol. 160: 87-94 [Abstract] [Full Text]  
• Johansson, M. H., Bieberich, C., Jay, G., Karre, K., Hoglund, P. (1997). Natural Killer Cell Tolerance in Mice with Mosaic Expression of Major Histocompatibility Complex Class I Transgene. J. Exp. Med. 186: 353-364 [Abstract] [Full Text]  
• Held, W., Raulet, D. H. (1997). Ly49A Transgenic Mice Provide Evidence for a Major Histocompatibility Complex-dependent Education Process in Natural Killer Cell Development. J. Exp. Med. 185: 2079-2088 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS