Thymocytes can become mature T cells without passing through the CD4+ CD8+, double-positive stage

doi:10.1084/jem.184.5.1619

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Thymocytes can become mature T cells without passing through the CD4+ CD8+, double-positive stage

CP Liu, JW Kappler and P Marrack
Howard Hughes Medical Institute, Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA.

T cells bearing the class II-restricted, DO-T cell receptor (TCR) are CD4+ if their thymocyte precursors are positively selected on the class II protein, IAd, but they are almost all CD4- after positive selection on a class II for which they have higher avidity, IAb. DO-TCR+ T cells mature in H-2b mice lacking CD4. CD4- DO-TCR+ T cells appear in H-2b mice at the same rate as their CD4+ counterparts appear in H-2d animals, suggesting that the CD4- cells are not the product of some minor pathway of thymocyte development and selection. In H-2b CD4 knock out mice expressing human CD2 under the control of the mouse CD4 promoter, mature DO-TCR+ cells did not express human CD2. These results suggest that the CD4-CD8-, DO-TCR+ mature T cells have developed without ever passing through the equivalent of a CD4+,CD8+ stage. The early expression of alpha/beta receptors (TCRs) on thymocytes in TCR transgenic mice may allow maturation of this type. Passage through the equivalent of the CD4+ CD8+, double-positive stage is not essential for differentiation of thymocytes into mature T cells.
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Illes, Z., Waldner, H., Reddy, J., Anderson, A. C., Sobel, R. A., Kuchroo, V. K. (2007). Modulation of CD4 co-receptor limits spontaneous autoimmunity when high-affinity transgenic TCR specific for self-antigen is expressed on a genetically resistant background. Int Immunol 19: 1235-1248 [Abstract] [Full Text]
Geraldes, P., Rebrovich, M., Herrmann, K., Wong, J., Jack, H.-M., Wabl, M., Cascalho, M. (2007). Ig Heavy Chain Promotes Mature B Cell Survival in the Absence of Light Chain. J. Immunol. 179: 1659-1668 [Abstract] [Full Text]
Leng, Q., Ge, Q., Nguyen, T., Eisen, H. N., Chen, J. (2007). Stage-dependent reactivity of thymocytes to self-peptide-MHC complexes. Proc. Natl. Acad. Sci. USA 104: 5038-5043 [Abstract] [Full Text]
Inoue, J., Kanefuji, T., Okazuka, K., Watanabe, H., Mishima, Y., Kominami, R. (2006). Expression of TCR{alpha}beta Partly Rescues Developmental Arrest and Apoptosis of {alpha}beta T cells in Bcl11b-/- Mice. J. Immunol. 176: 5871-5879 [Abstract] [Full Text]
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Grandjean, I., Duban, L., Bonney, E. A., Corcuff, E., Di Santo, J. P., Matzinger, P., Lantz, O. (2003). Are Major Histocompatibility Complex Molecules Involved in the Survival of Naive CD4+ T Cells?. J. Exp. Med. 198: 1089-1102 [Abstract] [Full Text]
DeRyckere, D., Mann, D. L., DeGregori, J. (2003). Characterization of Transcriptional Regulation During Negative Selection In Vivo. J. Immunol. 171: 802-811 [Abstract] [Full Text]
Viret, C., Janeway, C. A. Jr. (2003). Self-Specific MHC Class II-Restricted CD4-CD8- T Cells That Escape Deletion and Lack Regulatory Activity. J. Immunol. 170: 201-209 [Abstract] [Full Text]
Yang, L., Qin, X.-F., Baltimore, D., Van Parijs, L. (2002). Generation of functional antigen-specific T cells in defined genetic backgrounds by retrovirus-mediated expression of TCR cDNAs in hematopoietic precursor cells. Proc. Natl. Acad. Sci. USA 99: 6204-6209 [Abstract] [Full Text]
Suto, A., Nakajima, H., Ikeda, K., Kubo, S., Nakayama, T., Taniguchi, M., Saito, Y., Iwamoto, I. (2002). CD4+CD25+ T-cell development is regulated by at least 2 distinct mechanisms. Blood 99: 555-560 [Abstract] [Full Text]
Nakayama, T., Kasprowicz, D. J., Yamashita, M., Schubert, L. A., Gillard, G., Kimura, M., Didierlaurent, A., Koseki, H., Ziegler, S. F. (2002). The Generation of Mature, Single-Positive Thymocytes In Vivo Is Dysregulated by CD69 Blockade or Overexpression. J. Immunol. 168: 87-94 [Abstract] [Full Text]
Mora, A. L., Stanley, S., Armistead, W., Chan, A. C., Boothby, M. (2001). Inefficient ZAP-70 Phosphorylation and Decreased Thymic Selection In Vivo Result from Inhibition of NF-{kappa}B/Rel. J. Immunol. 167: 5628-5635 [Abstract] [Full Text]
Baur, N., Nerz, G., Nil, A., Eichmann, K. (2001). Expression and selection of productively rearranged TCR{beta} VDJ genes are sequentially regulated by CD3 signaling in the development of NK1.1+ {alpha}{beta} T cells. Int Immunol 13: 1031-1042 [Abstract] [Full Text]
Azzam, H. S., DeJarnette, J. B., Huang, K., Emmons, R., Park, C.-S., Sommers, C. L., El-Khoury, D., Shores, E. W., Love, P. E. (2001). Fine Tuning of TCR Signaling by CD5. J. Immunol. 166: 5464-5472 [Abstract] [Full Text]
Lacorazza, H. D., Tucek-Szabo, C., Vasovic, L. V., Remus, K., Nikolich-Zugich, J. (2001). Premature TCR{{alpha}}{{beta}} Expression and Signaling in Early Thymocytes Impair Thymocyte Expansion and Partially Block Their Development. J. Immunol. 166: 3184-3193 [Abstract] [Full Text]
Kraj, P., Pacholczyk, R., Ignatowicz, L. (2001). {{alpha}}{{beta}}TCRs Differ in the Degree of Their Specificity for the Positively Selecting MHC/Peptide Ligand. J. Immunol. 166: 2251-2259 [Abstract] [Full Text]
Love, P. E., Lee, J., Shores, E. W. (2000). Critical Relationship Between TCR Signaling Potential and TCR Affinity During Thymocyte Selection. J. Immunol. 165: 3080-3087 [Abstract] [Full Text]
Terrence, K., Pavlovich, C. P., Matechak, E. O., Fowlkes, B.J. (2000). Premature Expression of T Cell Receptor (TCR){alpha}{beta} Suppresses TCR{gamma}{delta} Gene Rearrangement but Permits Development of {gamma}{delta} Lineage T Cells. J. Exp. Med. 192: 537-548 [Abstract] [Full Text]
Pena-Rossi, C., Zuckerman, L. A., Strong, J., Kwan, J., Ferris, W., Chan, S., Tarakhovsky, A., Beyers, A. D., Killeen, N. (1999). Negative Regulation of CD4 Lineage Development and Responses by CD5. J. Immunol. 163: 6494-6501 [Abstract] [Full Text]
Zhu, J. W., DeRyckere, D., Li, F. X., Wan, Y. Y., DeGregori, J. (1999). A Role for E2F1 in the Induction of ARF, p53, and Apoptosis during Thymic Negative Selection. Cell Growth Differ. 10: 829-838 [Abstract] [Full Text]
Mixter, P. F., Russell, J. Q., Morrissette, G. J., Charland, C., Aleman-Hoey, D., Budd, R. C. (1999). A Model for the Origin of TCR-{alpha}{beta}+ CD4-CD8- B220+ Cells Based on High Affinity TCR Signals. J. Immunol. 162: 5747-5756 [Abstract] [Full Text]
Nakajima, H., Leonard, W. J. (1999). Role of Bcl-2 in {alpha}{beta} T Cell Development in Mice Deficient in the Common Cytokine Receptor {gamma}-Chain: The Requirement for Bcl-2 Differs Depending on the TCR/MHC Affinity. J. Immunol. 162: 782-790 [Abstract] [Full Text]
Azzam, H. S., Grinberg, A., Lui, K., Shen, H., Shores, E. W., Love, P. E. (1998). CD5 Expression Is Developmentally Regulated By T Cell Receptor (TCR) Signals and TCR Avidity. J. Exp. Med. 188: 2301-2311 [Abstract] [Full Text]
Russell, J. Q., Morrissette, G. J., Weidner, M., Vyas, C., Aleman-Hoey, D., Budd, R. C. (1998). Liver Damage Preferentially Results from CD8+ T Cells Triggered by High Affinity Peptide Antigens. J. Exp. Med. 188: 1147-1157 [Abstract] [Full Text]
Iwabuchi, C., Iwabuchi, K., Nakagawa, K.-i., Takayanagi, T., Nishihori, H., Tone, S., Ogasawara, K., Good, R. A., Onoe, K. (1998). Intrathymic selection of NK1.1+alpha /beta T cell antigen receptor (TCR)+ cells in transgenic mice bearing TCR specific for chicken ovalbumin and restricted to I-Ad. Proc. Natl. Acad. Sci. USA 95: 8199-8204 [Abstract] [Full Text]
Garside, P., Ingulli, E., Merica, R. R., Johnson, J. G., Noelle, R. J., Jenkins, M. K. (1998). Visualization of Specific B and T Lymphocyte Interactions in the Lymph Node. Science 281: 96-99 [Abstract] [Full Text]
Auphan, N., Simon, A. K., Asnagli, H., Phillips, R. J., Rincon, M., Ghosh, S., Flavell, R. A., Schmitt-Verhulst, A.-M. (1998). Consequences of Intrathymic TCR Engagement by Partial Agonist on Selection Events and Peripheral T Cell Activation Program. J. Immunol. 160: 4810-4821 [Abstract] [Full Text]
Williams, O., Tarazona, R., Wack, A., Harker, N., Roderick, K., Kioussis, D. (1998). Interactions with multiple peptide ligands determine the fate of developing thymocytes. Proc. Natl. Acad. Sci. USA 95: 5706-5711 [Abstract] [Full Text]
Reiner, S. L., Fowell, D. J., Moskowitz, N. H., Swier, K., Brown, D. R., Brown, C. R., Turck, C. W., Scott2, P. A., Killeen, N., Locksley3, R. M. (1998). Control of Leishmania major by a Monoclonal {alpha}{beta} T Cell Repertoire. J. Immunol. 160: 884-889 [Abstract] [Full Text]
Liu, C.-P., Lin, W.-J., Huang, M., Kappler, J. W., Marrack, P. (1997). Development and function of T cells in T cell antigen receptor/CD3 zeta �knockout mice reconstituted with Fcvarepsilon RI�gamma. Proc. Natl. Acad. Sci. USA 94: 616-621 [Abstract] [Full Text]
Wang, R., Wang-Zhu, Y., Grey, H. (2002). Interactions between double positive thymocytes and high affinity ligands presented by cortical epithelial cells generate double negative thymocytes with T cell regulatory activity. Proc. Natl. Acad. Sci. USA 99: 2181-2186 [Abstract] [Full Text]