Loss of a unique tumor antigen by cytotoxic T lymphocyte immunoselection from a 3-methylcholanthrene-induced mouse sarcoma reveals secondary unique and shared antigens

doi:10.1084/jem.184.2.441

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Dudley, M. E.
	Articles by Roopenian, D. C.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Dudley, M. E.
	Articles by Roopenian, D. C.


Social Bookmarking

	What's this?

ARTICLES

Loss of a unique tumor antigen by cytotoxic T lymphocyte immunoselection from a 3-methylcholanthrene-induced mouse sarcoma reveals secondary unique and shared antigens

ME Dudley and DC Roopenian
Jackson Laboratory, Bar Harbor, Maine 04609, USA.

Most chemically induced tumors of mice express unique antigens that can be recognized by cytotoxic T lymphocytes (CTL) and thereby mediate tumor rejection. The number of different antigens expressed by a single tumor and their interplay during immunization and rejection are largely unexplored. We used CTL clones specific to individual tumor antigens to examine the number and distribution of CTL antigens expressed by cell lines derived from 3-methylcholanthrene-induced sarcomas of (C57BL/6J X SPRET/Ei)F1 mice. Each tumor cell line expressed one or more antigens that were unique, that is, not detected on cell lines from independent sarcomas. Immunoselection against an immunodominant antigen produced both major histocompatibility complex class I antigen and unique tumor antigen loss variants. Immunization of mice with antigen-negative immunoselected variants resulted in CTL that recognized additional antigens that were also expressed by the progenitor tumor. Some CTL recognized additional unique tumor antigen(s); other CTL recognized a shared antigen expressed not only by the immunizing cell line, but also by independent sarcoma cell lines and untransformed myoblastoid cell lines. CTL that recognized the shared antigen were also recovered from mice immunized in vivo with an untransformed myoblastoid cell line. These findings support a model of immunodominance among chemically induced tumor antigens in which shared antigens are masked by unique immunodominant antigens.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Shanker, A., Verdeil, G., Buferne, M., Inderberg-Suso, E.-M., Puthier, D., Joly, F., Nguyen, C., Leserman, L., Auphan-Anezin, N., Schmitt-Verhulst, A.-M. (2007). CD8 T Cell Help for Innate Antitumor Immunity. J. Immunol. 179: 6651-6662 [Abstract] [Full Text]
Sensi, M., Anichini, A. (2006). Unique Tumor Antigens: Evidence for Immune Control of Genome Integrity and Immunogenic Targets for T Cell-Mediated Patient-Specific Immunotherapy. Clin. Cancer Res. 12: 5023-5032 [Abstract] [Full Text]
Parmiani, G., Testori, A., Maio, M., Castelli, C., Rivoltini, L., Pilla, L., Belli, F., Mazzaferro, V., Coppa, J., Patuzzo, R., Sertoli, M. R., Hoos, A., Srivastava, P. K., Santinami, M. (2004). Heat Shock Proteins and Their Use as Anticancer Vaccines. Clin. Cancer Res. 10: 8142-8146 [Full Text]
Matsui, K., O'Mara, L. A., Allen, P. M. (2003). Successful elimination of large established tumors and avoidance of antigen-loss variants by aggressive adoptive T cell immunotherapy. Int Immunol 15: 797-805 [Abstract] [Full Text]
Serreze, D. V., Pierce, M. A., Post, C. M., Chapman, H. D., Savage, H., Bronson, R. T., Rothman, P. B., Cox, G. A. (2003). Paralytic Autoimmune Myositis Develops in Nonobese Diabetic Mice Made Th1 Cytokine-Deficient by Expression of an IFN-{gamma} Receptor {beta}-Chain Transgene. J. Immunol. 170: 2742-2749 [Abstract] [Full Text]
Akilesh, S., Dudley, M. E., Eden, P. A., Roopenian, D. C. (2001). Efficient Chromosomal Mapping of a Methylcholanthrene- Induced Tumor Antigen by CTL Immunoselection. J. Immunol. 167: 5143-5149 [Abstract] [Full Text]
Matsuo, M., Wada, H., Honda, S., Tawara, I., Uenaka, A., Kanematsu, T., Nakayama, E. (1999). Expression of Multiple Unique Rejection Antigens on Murine Leukemia BALB/c RL[IMAGE]1 and the Role of Dominant Akt Antigen for Tumor Escape. J. Immunol. 162: 6420-6425 [Abstract] [Full Text]
Eden, P. A., Christianson, G. J., Fontaine, P., Wettstein, P. J., Perreault, C., Roopenian, D. C. (1999). Biochemical and Immunogenetic Analysis of an Immunodominant Peptide (B6dom1) Encoded by the Classical H7 Minor Histocompatibility Locus. J. Immunol. 162: 4502-4510 [Abstract] [Full Text]
Pion, S., Christianson, G. J., Fontaine, P., Roopenian, D. C., Perreault, C. (1999). Shaping the Repertoire of Cytotoxic T-Lymphocyte Responses: Explanation for the Immunodominance Effect Whereby Cytotoxic T Lymphocytes Specific for Immunodominant Antigens Prevent Recognition of Nondominant Antigens. Blood 93: 952-962 [Abstract] [Full Text]
Klein, G. (1997). Rejection antigens in chemically induced�tumors. Proc. Natl. Acad. Sci. USA 94: 5991-5992 [Full Text]
Ikeda, H., Ohta, N., Furukawa, K., Miyazaki, H., Wang, L., Furukawa, K., Kuribayashi, K., Old, L. J., Shiku, H. (1997). Mutated mitogen-activated protein kinase: A tumor rejection antigen of mouse�sarcoma. Proc. Natl. Acad. Sci. USA 94: 6375-6379 [Abstract] [Full Text]