Developmental arrest of NK1.1+ T cell antigen receptor (TCR)- alpha/beta+ T cells and expansion of NK1.1+ TCR-gamma/delta+ T cell development in CD3 zeta-deficient mice

doi:10.1084/jem.182.3.891

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Arase, H.
	Articles by Saito, T.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Arase, H.
	Articles by Saito, T.


Social Bookmarking

	What's this?

ARTICLES

Developmental arrest of NK1.1+ T cell antigen receptor (TCR)- alpha/beta+ T cells and expansion of NK1.1+ TCR-gamma/delta+ T cell development in CD3 zeta-deficient mice

H Arase, S Ono, N Arase, SY Park, K Wakizaka, H Watanabe, H Ohno and T Saito
Division of Molecular Genetics, Chiba University School of Medicine, Japan.

The relationship between the structure of the T cell antigen receptor (TCR)-CD3 complex and development of NK1.1+ T cells was investigated. The TCR complex of freshly isolated NK1.1+ TCR-alpha/beta+ thymocytes contained CD3 zeta homodimers and CD zeta-FcR gamma heterodimers, whereas that of the majority of NK1.1- T cells did not contain FcR gamma. The function of CD3 zeta and FcR gamma in the development of NK1.1+ T cells was determined by analyzing CD3 zeta- and FcR gamma- deficient mice. The NK1.1+ T cells from wild-type and CD3 zeta- deficient mice had equal levels of CD3 expression. However, the development of NK1.1+ TCR-alpha/beta+ T cells was almost completely disrupted in thymus and spleen in CD3 zeta-deficient mice, whereas no alteration was observed in FcR gamma-deficient mice. In contrast, the number of novel NK1.1+ TCR-gamma/delta+ thymocytes expressing a surface phenotype similar to NK1.1+ TCR-alpha/beta+ thymocytes increased approximately six times in CD3 zeta-deficient mice. These findings establish the distinct roles of the CD3 zeta chain in the development of the following different thymic T cell compartments: NK1.1- TCR+, NK1.1+ TCR-alpha/beta+, and NK1.1+ TCR-gamma/delta+ thymocytes, which cannot be replaced by CD3 eta or FcR gamma chains.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Thomson, C. W., Teft, W. A., Chen, W., Lee, B. P.-L., Madrenas, J., Zhang, L. (2006). FcR{gamma} Presence in TCR Complex of Double-Negative T Cells Is Critical for Their Regulatory Function. J. Immunol. 177: 2250-2257 [Abstract] [Full Text]
Taylor, K. N., Shinde Patil, V. R., Colson, Y. L. (2006). Reconstitution of Allogeneic Hemopoietic Stem Cells: The Essential Role of FcR{gamma} and the TCR beta-Chain-FCp33 Complex. J. Immunol. 177: 1444-1450 [Abstract] [Full Text]
Gerber, D., Boucontet, L., Pereira, P. (2004). Early Expression of a Functional TCR{beta} Chain Inhibits TCR{gamma} Gene Rearrangements without Altering the Frequency of TCR{gamma}{delta} Lineage Cells. J. Immunol. 173: 2516-2523 [Abstract] [Full Text]
Grigoriadou, K., Boucontet, L., Pereira, P. (2003). Most IL-4-Producing {gamma}{delta} Thymocytes of Adult Mice Originate from Fetal Precursors. J. Immunol. 171: 2413-2420 [Abstract] [Full Text]
Van Beneden, K., De Creus, A., Stevenaert, F., Debacker, V., Plum, J., Leclercq, G. (2002). Expression of Inhibitory Receptors Ly49E and CD94/NKG2 on Fetal Thymic and Adult Epidermal TCR V{gamma}3 Lymphocytes. J. Immunol. 168: 3295-3302 [Abstract] [Full Text]
Baur, N., Nerz, G., Nil, A., Eichmann, K. (2001). Expression and selection of productively rearranged TCR{beta} VDJ genes are sequentially regulated by CD3 signaling in the development of NK1.1+ {alpha}{beta} T cells. Int Immunol 13: 1031-1042 [Abstract] [Full Text]
Petersson, K., Ivars, F. (2001). Early TCR {{alpha}}{{beta}} Expression Promotes Maturation of T Cells Expressing Fc{{epsilon}}RI{{gamma}} Containing TCR/CD3 Complexes. J. Immunol. 166: 6616-6624 [Abstract] [Full Text]
Iwabuchi, K., Iwabuchi, C., Tone, S., Itoh, D., Tosa, N., Negishi, I., Ogasawara, K., Uede, T., Onoe, K. (2001). Defective development of NK1.1+ T-cell antigen receptor {alpha}{beta}+ cells in zeta-associated protein 70 null mice with an accumulation of NK1.1+ CD3{-} NK-like cells in the thymus. Blood 97: 1765-1775 [Abstract] [Full Text]
Liu, Z.-X., Govindarajan, S., Okamoto, S., Dennert, G. (2001). Fas-Mediated Apoptosis Causes Elimination of Virus-Specific Cytotoxic T Cells in the Virus-Infected Liver. J. Immunol. 166: 3035-3041 [Abstract] [Full Text]
Arase, H., Suenaga, T., Arase, N., Kimura, Y., Ito, K., Shiina, R., Ohno, H., Saito, T. (2001). Negative Regulation of Expression and Function of Fc{{gamma}}RIII by CD3{{zeta}} in Murine NK Cells. J. Immunol. 166: 21-25 [Abstract] [Full Text]
Tamura, T., Kunimatsu, T., Yee, S.-T., Igarashi, O., Utsuyama, M., Tanaka, S., Miyazaki, S.-i., Hirokawa, K., Nariuchi, H. (2000). Molecular mechanism of the impairment in activation signal transduction in CD4+ T cells from old mice. Int Immunol 12: 1205-1215 [Abstract] [Full Text]
Gadue, P., Morton, N., Stein, P. L. (1999). The Src Family Tyrosine Kinase Fyn Regulates Natural Killer T Cell Development. J. Exp. Med. 190: 1189-1196 [Abstract] [Full Text]
Shores, E. W., Ono, M., Kawabe, T., Sommers, C. L., Tran, T., Lui, K., Udey, M. C., Ravetch, J., Love, P. E. (1998). T Cell Development in Mice Lacking All T Cell Receptor zeta �Family Members (zeta , eta , and Fcepsilon RIgamma ). J. Exp. Med. 187: 1093-1101 [Abstract] [Full Text]
Arase, N., Arase, H., Park, S. Y., Ohno, H., Ra, C., Saito, T. (1997). Association with FcRgamma Is Essential for Activation Signal through NKR-P1 (CD161) in Natural Killer (NK) Cells and NK1.1+ T Cells. J. Exp. Med. 186: 1957-1963 [Abstract] [Full Text]
Lantz, O., Sharara, L. I., Tilloy, F., Andersson, A., DiSanto, J. P. (1997). Lineage Relationships and Differentiation of Natural Killer (NK) T Cells: Intrathymic Selection and Interleukin (IL)-4 Production in the Absence of NKR-P1 and Ly49 Molecules. J. Exp. Med. 185: 1395-1402 [Abstract] [Full Text]