Cloning and characterization of 5E6(Ly-49C), a receptor molecule expressed on a subset of murine natural killer cells

doi:10.1084/jem.182.2.305

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Cloning and characterization of 5E6(Ly-49C), a receptor molecule expressed on a subset of murine natural killer cells

ER Stoneman, M Bennett, J An, KA Chesnut, EK Wakeland, JB Scheerer, MJ Siciliano, V Kumar and PA Mathew
Department of Pathology, University of Texas Southwestern Medical Center at Dallas 75235, USA.

5E6 is a cell surface molecule expressed on a subpopulation of murine natural killer (NK) cells that are involved in the specific rejection of H-2d or H-2f (hemopoietic histocompatibility determinant 2) bone marrow cell grafts. Here, we isolated and cloned the gene encoding 5E6 and determined the nucleotide sequence of the cDNA. 5E6 is nearly identical to Ly-49C; the deduced amino acid sequence reveals a polypeptide of 266 amino acids with a molecular weight of 31,284 that contains multiple cysteine residues to explain its disulfide-linked homodimer structure and five potential N-linked glycosylation sites. 5E6 is a type II integral membrane protein with an extracellular carbohydrate recognition domain characteristic of C-type (Ca(2+)- dependent) animal lectins. Chromosomal mapping indicates that 5E6 is located within the NK gene complex on chromosome 6. The sequence of 5E6 mRNA and the degree of glycosylation of 5E6 protein are under genetic control. Immunoprecipitation before removal of N-linked sugars reveals different size molecules. There are several nucleotide differences among BALB/c, B6, and NZB mRNAs; however, none of them would be expected to affect N-glycosylation. Of particular interest are two findings: (a) BALB/c, B6, and (BALB/c x B6)F1 5E6 reduced molecules are approximately 65, 54, and 54 kD, and (b) the cDNA sequence of (BALB/c x B6)F1 is identical to B6. Thus, there appears to be allelic exclusion of 5E6 expression that may be related to the ability of F1 hybrid mice to reject parental H-2d bone marrow cell grafts.
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Lian, R. H., Li, Y., Kubota, S., Mager, D. L., Takei, F. (1999). Recognition of Class I MHC by NK Receptor Ly-49C: Identification of Critical Residues. J. Immunol. 162: 7271-7276 [Abstract] [Full Text]
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Idris, A. H., Iizuka, K., Smith, H. R.C., Scalzo, A. A., Yokoyama, W. M. (1998). Genetic Control Of Natural Killing and In Vivo Tumor Elimination by the Chok Locus. J. Exp. Med. 188: 2243-2256 [Abstract] [Full Text]
Sundback, J., Nakamura, M. C., Waldenstrom, M., Niemi, E. C., Seaman, W. E., Ryan, J. C., Karre, K. (1998). The {alpha}2 Domain of H-2Dd Restricts the Allelic Specificity of the Murine NK Cell Inhibitory Receptor Ly-49A. J. Immunol. 160: 5971-5978 [Abstract] [Full Text]
Ho, E. L., Heusel, J. W., Brown, M. G., Matsumoto, K., Scalzo, A. A., Yokoyama, W. M. (1998). Murine Nkg2d and Cd94 are clustered within the natural killer complex and are expressed independently in natural killer cells. Proc. Natl. Acad. Sci. USA 95: 6320-6325 [Abstract] [Full Text]
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Wang, J., Whitman, M. C., Natarajan, K., Tormo, J., Mariuzza, R. A., Margulies, D. H. (2002). Binding of the Natural Killer Cell Inhibitory Receptor Ly49A to Its Major Histocompatibility Complex Class I Ligand. CRUCIAL CONTACTS INCLUDE BOTH H-2Dd AND beta 2-MICROGLOBULIN. J. Biol. Chem. 277: 1433-1442 [Abstract] [Full Text]