Upregulation of surface markers on dying thymocytes

doi:10.1084/jem.181.2.649

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Upregulation of surface markers on dying thymocytes

H Kishimoto, CD Surh and J Sprent
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.

Using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) to detect cells undergoing early apoptosis, we have defined the surface markers expressed on CD4+CD8+ thymocytes undergoing spontaneous or steroid-induced apoptosis in tissue culture. Some surface markers, e.g., CD4, CD8, and heat stable antigen, are downregulated on apoptotic thymocytes. Surprisingly, however, other markers are upregulated; this applies to T cell receptor beta/CD3, CD69, and CD25 expression. Upregulation of these markers is restricted to a discrete subset of apoptotic cells.
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Prehaud, C., Lay, S., Dietzschold, B., Lafon, M. (2003). Glycoprotein of Nonpathogenic Rabies Viruses Is a Key Determinant of Human Cell Apoptosis. J. Virol. 77: 10537-10547 [Abstract] [Full Text]
Na, S.-Y., Patra, A., Scheuring, Y., Marx, A., Tolaini, M., Kioussis, D., Hemmings, B., Hunig, T., Bommhardt, U. (2003). Constitutively Active Protein Kinase B Enhances Lck and Erk Activities and Influences Thymocyte Selection and Activation. J. Immunol. 171: 1285-1296 [Abstract] [Full Text]
Viret, C., He, X., Janeway, C. A. Jr. (2003). Altered positive selection due to corecognition of floppy peptide/MHC II conformers supports an integrative model of thymic selection. Proc. Natl. Acad. Sci. USA 100: 5354-5359 [Abstract] [Full Text]
Zhan, Y., Purton, J. F., Godfrey, D. I., Cole, T. J., Heath, W. R., Lew, A. M. (2003). Without peripheral interference, thymic deletion is mediated in a cohort of double-positive cells without classical activation. Proc. Natl. Acad. Sci. USA 100: 1197-1202 [Abstract] [Full Text]
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Bassiri, H., Carding, S. R. (2001). A Requirement for IL-2/IL-2 Receptor Signaling in Intrathymic Negative Selection. J. Immunol. 166: 5945-5954 [Abstract] [Full Text]
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Ludviksson, B. R., Ehrhardt, R. O., Strober, W. (1999). Role of IL-12 in Intrathymic Negative Selection. J. Immunol. 163: 4349-4359 [Abstract] [Full Text]
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Bakker, T. R., Renno, T., Jongeneel, C. V. (1999). Impaired Fetal Thymocyte Development After Efficient Adenovirus-Mediated Inhibition of NF-{kappa}B Activation. J. Immunol. 162: 3456-3462 [Abstract] [Full Text]
McGargill, M. A., Hogquist, K. A. (1999). Antigen-Induced Coreceptor Down-Regulation on Thymocytes Is Not a Result of Apoptosis. J. Immunol. 162: 1237-1245 [Abstract] [Full Text]
Fatenejad, S., Peng, S. L., Disorbo, O., Craft, J. (1998). Central T Cell Tolerance in Lupus-Prone Mice: Influence of Autoimmune Background and the lpr Mutation. J. Immunol. 161: 6427-6432 [Abstract] [Full Text]
Levelt, C. N., Mizoguchi, E., Huang, X., Zacks, R., Bhan, A. K., Tonegawa, S. (1998). Inhibition of intrathymic T cell development by expression of a transgenic antagonist peptide. Proc. Natl. Acad. Sci. USA 95: 14349-14354 [Abstract] [Full Text]
Rincon, M., Whitmarsh, A., Yang, D. D., Weiss, L., Derijard, B., Jayaraj, P., Davis, R. J., Flavell, R. A. (1998). The JNK Pathway Regulates the In Vivo Deletion of Immature CD4+CD8+ Thymocytes. J. Exp. Med. 188: 1817-1830 [Abstract] [Full Text]
Belz, G. T., Altman, J. D., Doherty, P. C. (1998). Characteristics of virus-specific CD8+ T cells in the liver during the control and resolution phases of influenza pneumonia. Proc. Natl. Acad. Sci. USA 95: 13812-13817 [Abstract] [Full Text]
Choi, E. Y., Park, W. S., Jung, K. C., Kim, S. H., Kim, Y. Y., Lee, W. J., Park, S. H. (1998). Engagement of CD99 Induces Up-Regulation of TCR and MHC Class I and II Molecules on the Surface of Human Thymocytes. J. Immunol. 161: 749-754 [Abstract] [Full Text]
Bruno, T. F., Buser, D. E., Syme, R. M., Woods, D. E., Mody, C. H. (1998). Pseudomonas aeruginosa Exoenzyme S Is a Mitogen but Not a Superantigen for Human T Lymphocytes. Infect. Immun. 66: 3072-3079 [Abstract] [Full Text]
Ramanathan, S., Norwich, K., Poussier, P. (1998). Antigen Activation Rescues Recent Thymic Emigrants from Programmed Cell Death in the BB Rat. J. Immunol. 160: 5757-5764 [Abstract] [Full Text]
Kishimoto, H., Surh, C. D., Sprent, J. (1998). A Role for Fas in Negative Selection of Thymocytes In Vivo. J. Exp. Med. 187: 1427-1438 [Abstract] [Full Text]
Merkenschlager, M., Graf, D., Lovatt, M., Bommhardt, U., Zamoyska, R., Fisher, A. G. (1997). How Many Thymocytes Audition for Selection?. J. Exp. Med. 186: 1149-1158 [Abstract] [Full Text]
Kishimoto, H., Sprent, J. (1997). Negative Selection in the Thymus Includes Semimature T Cells. J. Exp. Med. 185: 263-272 [Abstract] [Full Text]
Taylor, D, Badiani, P, Weston, K (1996). A dominant interfering Myb mutant causes apoptosis in T cells.. Genes Dev. 10: 2732-2744 [Abstract]