Independent regulation of DNA recombination and immunoglobulin (Ig) secretion during isotype switching to IgG1 and IgE

doi:10.1084/jem.179.6.1877

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Purkerson, J. M.
	Articles by Isakson, P. C.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Purkerson, J. M.
	Articles by Isakson, P. C.


Social Bookmarking

	What's this?

ARTICLES

Independent regulation of DNA recombination and immunoglobulin (Ig) secretion during isotype switching to IgG1 and IgE

JM Purkerson and PC Isakson
Searle Research and Development, Monsanto Company, St. Louis, Missouri 63198.

Induction of switch recombination to the gamma 1 and epsilon immunoglobulin (Ig) heavy chain loci was examined in B cells preactivated with anti-Ig (B lymphoblasts). In B lymphoblasts cultured with interleukin 4 (IL-4), IL-5 induced the accumulation of S micro-S gamma 1 rearrangements, but not epsilon recombination. Thus, IL-5 facilitates switch recombination directed to the gamma 1 heavy chain locus by IL-4, but additional signals are required to drive rearrangements to epsilon. Lipopolysaccharide (LPS), in the presence of IL-4, induced the accumulation of both S micro-S gamma 1 and S micro-S epsilon rearrangements, and cells treated with LPS exhibited 40-50-fold more S micro-S gamma 1 rearrangements than cells cultured with IL-5. Induction of switch recombination was not always associated with secretion of the respective Ig isotype, since concentrations of IL-4 that were sufficient to direct switch recombination to gamma 1 and epsilon in blasts treated with LPS failed to elicit secretion of IgG1 and IgE. These results demonstrate differential requirements for switch recombination to the gamma 1 and epsilon loci, as well as independent regulation of Ig gene rearrangement and secretion of each isotype.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Harada, M., Magara-Koyanagi, K., Watarai, H., Nagata, Y., Ishii, Y., Kojo, S., Horiguchi, S., Okamoto, Y., Nakayama, T., Suzuki, N., Yeh, W.-C., Akira, S., Kitamura, H., Ohara, O., Seino, K.-i., Taniguchi, M. (2006). IL-21-induced B{varepsilon} cell apoptosis mediated by natural killer T cells suppresses IgE responses. J. Exp. Med. 203: 2929-2937 [Abstract] [Full Text]
Ehigiator, H. N., Stadnyk, A. W., Lee, T. D. G. (2000). Extract of Nippostrongylus brasiliensis Stimulates Polyclonal Type-2 Immunoglobulin Response by Inducing De Novo Class Switch. Infect. Immun. 68: 4913-4922 [Abstract] [Full Text]
Linehan, L. A., Warren, W. D., Thompson, P. A., Grusby, M. J., Berton, M. T. (1998). STAT6 Is Required for IL-4-Induced Germline Ig Gene Transcription and Switch Recombination. J. Immunol. 161: 302-310 [Abstract] [Full Text]
Purkerson, J. M., Parker, D. C. (1998). Differential Coupling of Membrane Ig and CD40 to the Extracellularly Regulated Kinase Signaling Pathway. J. Immunol. 160: 2121-2129 [Abstract] [Full Text]