Comparison of peptides bound to spleen and thymus class II

doi:10.1084/jem.178.6.2173

This Article

	Full Text (PDF, 1155K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Marrack, P.
	Articles by Freed, J. H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Marrack, P.
	Articles by Freed, J. H.


Social Bookmarking

	What's this?

ARTICLES

Comparison of peptides bound to spleen and thymus class II

P Marrack, L Ignatowicz, JW Kappler, J Boymel and JH Freed
Howard Hughes Medical Institute, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.

In the past we and others have suggested that positive selection of developing thymocytes may depend upon interaction between the alpha beta receptors on these cells and major histocompatibility complex (MHC) proteins bound to peptides found uniquely in the selecting tissue, thymus cortical epithelium. To test this hypothesis, peptides were isolated from MHC class II proteins of spleen, thymus cortical plus medullary epithelium, or thymus cortical epithelium alone. The results showed that the major peptides bound to class II on thymus cortical epithelium were also associated with spleen class II. Some peptides could only be detected in isolates from spleen, probably because of differences in the distribution or uptake of the donor proteins between spleen and thymus. Thus, although we found some tissue- specific distribution of self-peptides, our data suggest that there are no fundamental differences among these tissues in the occupancy of class II MHC by self-peptides. These results limit hypotheses which depend on a specialized mechanism of peptide generation and/or MHC class II loading to account for the positive selection of T cells on thymic cortical epithelium.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Felix, N. J., Suri, A., Walters, J. J., Horvath, S., Gross, M. L., Allen, P. M. (2006). I-Ep-Bound Self-Peptides: Identification, Characterization, and Role in Alloreactivity. J. Immunol. 176: 1062-1071 [Abstract] [Full Text]
Martinez-Soria, E., Ibnou-Zekri, N., Iwamoto, M., Santiago-Raber, M.-L., Kikuchi, S., Kosco-Vilbois, M., Izui, S. (2004). Epitope-Dependent Inhibition of T Cell Activation by the Ea Transgene: An Explanation for Transgene-Mediated Protection from Murine Lupus. J. Immunol. 173: 2842-2848 [Abstract] [Full Text]
Huseby, E. S., Crawford, F., White, J., Kappler, J., Marrack, P. (2003). Negative selection imparts peptide specificity to the mature T cell repertoire. Proc. Natl. Acad. Sci. USA 100: 11565-11570 [Abstract] [Full Text]
Lovitch, S. B., Walters, J. J., Gross, M. L., Unanue, E. R. (2003). APCs Present A{beta}k-Derived Peptides That Are Autoantigenic to Type B T Cells. J. Immunol. 170: 4155-4160 [Abstract] [Full Text]
Koonce, C. H., Wutz, G., Robertson, E. J., Vogt, A. B., Kropshofer, H., Bikoff, E. K. (2003). DM Loss in k Haplotype Mice Reveals Isotype-Specific Chaperone Requirements. J. Immunol. 170: 3751-3761 [Abstract] [Full Text]
Muntasell, A., Carrascal, M., Serradell, L., Veelen, P. v., Verreck, F., Koning, F., Raposo, G., Abian, J., Jaraquemada, D. (2002). HLA-DR4 Molecules in Neuroendocrine Epithelial Cells Associate to a Heterogeneous Repertoire of Cytoplasmic and Surface Self Peptides. J. Immunol. 169: 5052-5060 [Abstract] [Full Text]
Liu, X., Dai, S., Crawford, F., Fruge, R., Marrack, P., Kappler, J. (2002). Alternate interactions define the binding of peptides to the MHC molecule IAb. Proc. Natl. Acad. Sci. USA 99: 8820-8825 [Abstract] [Full Text]
Smith, K., Seddon, B., Purbhoo, M. A., Zamoyska, R., Fisher, A. G., Merkenschlager, M. (2001). Sensory Adaptation in Naive Peripheral CD4 T Cells. JEM 194: 1253-1262 [Abstract] [Full Text]
Sasada, T., Ghendler, Y., Neveu, J. M., Lane, W. S., Reinherz, E. L. (2001). A Naturally Processed Mitochondrial Self-Peptide in Complex with Thymic MHC Molecules Functions as a Selecting Ligand for a Viral-specific T Cell Receptor. JEM 194: 883-892 [Abstract] [Full Text]
Hogan, R. J., VanBeek, J., Broussard, D. R., Surman, S. L., Woodland, D. L. (2001). Identification of MHC Class II-Associated Peptides That Promote the Presentation of Toxic Shock Syndrome Toxin-1 to T Cells. J. Immunol. 166: 6514-6522 [Abstract] [Full Text]
Keech, C. L., Farris, A. D., Beroukas, D., Gordon, T. P., McCluskey, J. (2001). Cognate T Cell Help Is Sufficient to Trigger Anti-Nuclear Autoantibodies in Naive Mice. J. Immunol. 166: 5826-5834 [Abstract] [Full Text]
Kersh, G. J., Miley, M. J., Nelson, C. A., Grakoui, A., Horvath, S., Donermeyer, D. L., Kappler, J., Allen, P. M., Fremont, D. H. (2001). Structural and Functional Consequences of Altering a Peptide MHC Anchor Residue. J. Immunol. 166: 3345-3354 [Abstract] [Full Text]
Capone, M., Romagnoli, P., Beermann, F., MacDonald, H. R., van Meerwijk, J. P. M. (2001). Dissociation of thymic positive and negative selection in transgenic mice expressing major histocompatibility complex class I molecules exclusively on thymic cortical epithelial cells. Blood 97: 1336-1342 [Abstract] [Full Text]
Pacholczyk, R., Kraj, P., Ignatowicz, L. (2001). An Incremental Increase in the Complexity of Peptides Bound to Class II MHC Changes the Diversity of Positively Selected {{alpha}}{{beta}} TCRs. J. Immunol. 166: 2357-2363 [Abstract] [Full Text]
Latek, R. R., Petzold, S. J., Unanue, E. R. (2000). Hindering auxiliary anchors are potent modulators of peptide binding and selection by I-Ak class II molecules. Proc. Natl. Acad. Sci. USA 10.1073/pnas.210384197v1 [Abstract] [Full Text]
Basu, D., Horvath, S., Matsumoto, I., Fremont, D. H., Allen, P. M. (2000). Molecular Basis for Recognition of an Arthritic Peptide and a Foreign Epitope on Distinct MHC Molecules by a Single TCR. J. Immunol. 164: 5788-5796 [Abstract] [Full Text]
Freed, J. H., Marrs, A., VanderWall, J., Cohen, P. L., Eisenberg, R. A. (2000). MHC Class II-Bound Self Peptides from Autoimmune MRL/lpr Mice Reveal Potential T Cell Epitopes for Autoantibody Production in Murine Systemic Lupus Erythematosus. J. Immunol. 164: 4697-4705 [Abstract] [Full Text]
Robertson, J. M., Jensen, P. E., Evavold, B. D. (2000). DO11.10 and OT-II T Cells Recognize a C-Terminal Ovalbumin 323-339 Epitope. J. Immunol. 164: 4706-4712 [Abstract] [Full Text]
Chmielowski, B., Muranski, P., Kisielow, P., Ignatowicz, L. (2000). On the role of high- and low-abundance class II MHC-peptide complexes in the thymic positive selection of CD4+ T cells. Int Immunol 12: 67-72 [Abstract] [Full Text]
Bender, J., Mitchell, T., Kappler, J., Marrack, P. (1999). CD4+ T Cell Division in Irradiated Mice Requires Peptides Distinct from Those Responsible for Thymic Selection. JEM 190: 367-374 [Abstract] [Full Text]
Legge, K. L., Min, B., Pack, C., Caprio, J., Zaghouani, H. (1999). Differential Presentation of an Altered Peptide Within Fetal Central and Peripheral Organs Supports an Avidity Model for Thymic T Cell Development and Implies a Peripheral Readjustment for Activation. J. Immunol. 162: 5738-5746 [Abstract] [Full Text]
Unanue, E. R. (1999). Chemical Features of Peptide Selection by the Class II Histocompatibility Molecules. Am. J. Pathol. 154: 651-664 [Full Text]
Pearson, C. I., Gautam, A. M., Rulifson, I. C., Liblau, R. S., McDevitt, H. O. (1999). A small number of residues in the class II molecule I-Au confer the ability to bind the myelin basic protein peptide Ac1-11. Proc. Natl. Acad. Sci. USA 96: 197-202 [Abstract] [Full Text]
Baldwin, K. K., Reay, P. A., Wu, L., Farr, A., Davis, M. M. (1999). A T Cell Receptor-specific Blockade of Positive Selection. JEM 189: 13-24 [Abstract] [Full Text]
Carson, R. T., Desai, D. D., Vignali, K. M., Vignali2, D. A. A. (1999). Cutting Edge: Immunoregulation of Th Cells by Naturally Processed Peptide Antagonists. J. Immunol. 162: 1-4 [Abstract] [Full Text]
Delaney, J. R., Sykulev, Y., Eisen, H. N., Tonegawa, S. (1998). Differences in the level of expression of class I major histocompatibility complex proteins on thymic epithelial and dendritic cells influence the decision of immature thymocytes between positive and negative selection. Proc. Natl. Acad. Sci. USA 95: 5235-5240 [Abstract] [Full Text]
Liu, C.-P., Crawford, F., Marrack, P., Kappler, J. (1998). T cell positive selection by a high density, low affinity ligand. Proc. Natl. Acad. Sci. USA 95: 4522-4526 [Abstract] [Full Text]
Vacchio, M. S., Ashwell, J. D. (1997). Thymus-derived Glucocorticoids Regulate Antigen-specific Positive Selection. JEM 185: 2033-2038 [Abstract] [Full Text]
Barnaba, V., Sinigaglia, F. (1997). Molecular Mimicry and T Cell-mediated Autoimmune Disease. JEM 185: 1529-1532 [Full Text]
Lin, S.-Y., Ardouin, L., Gillet, A., Malissen, M., Malissen, B. (1997). The Single Positive T Cells Found in CD3-zeta /eta -/- Mice Overtly React with Self-Major Histocompatibility Complex Molecules upon Restoration of Normal Surface Density of T Cell Receptor-CD3 Complex. JEM 185: 707-716 [Abstract] [Full Text]
van Meerwijk, J. P.M., Marguerat, S., Lees, R. K., Germain, R. N., Fowlkes, B.J., MacDonald, H. R. (1997). Quantitative Impact of Thymic Clonal Deletion on the T Cell Repertoire. JEM 185: 377-384 [Abstract] [Full Text]
Phelps, R. G., Turner, A. N., Rees, A. J. (1996). Direct Identification of Naturally Processed Autoantigen-derived Peptides Bound to HLA-DR15. J. Biol. Chem. 271: 18549-18553 [Abstract] [Full Text]
Thibodeau, J, Cloutier, I, Lavoie, P., Labrecque, N, Mourad, W, Jardetzky, T, Sekaly, R. (1994). Subsets of HLA-DR1 molecules defined by SEB and TSST-1 binding. Science 266: 1874-1878 [Abstract]
Sebzda, E, Wallace, V., Mayer, J, Yeung, R., Mak, T., Ohashi, P. (1994). Positive and negative thymocyte selection induced by different concentrations of a single peptide. Science 263: 1615-1618 [Abstract]
Latek, R. R., Petzold, S. J., Unanue, E. R. (2000). Hindering auxiliary anchors are potent modulators of peptide binding and selection by I-Ak class II molecules. Proc. Natl. Acad. Sci. USA 97: 11460-11465 [Abstract] [Full Text]
Fremont, D. H., Dai, S., Chiang, H., Crawford, F., Marrack, P., Kappler, J. (2002). Structural Basis of Cytochrome c Presentation by IEk. JEM 195: 1043-1052 [Abstract] [Full Text]