The regulated expression of B lineage associated genes during B cell differentiation in bone marrow and fetal liver

doi:10.1084/jem.178.3.951

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The regulated expression of B lineage associated genes during B cell differentiation in bone marrow and fetal liver

YS Li, K Hayakawa and RR Hardy
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.

The expression of B lineage associated genes during early B cell differentiation stages is not firmly established. Using cell surface markers and multiparameter flow cytometry, bone marrow (BM) cells can be resolved into six fractions, representing sequential stages of development; i.e., pre-Pro-B, early Pro-B, late Pro-B/large Pre-B, small Pre-B, immature B, and mature B cells. Here we quantitate the levels of several B lineage associated genes in each of these fractions by RT-PCR, demonstrating different patterns of expression. We find that expression of terminal deoxynucleotidyl transferase (TdT), lambda 5, and VpreB is predominantly restricted to the Pro-B stages. Rag-1 and Rag-2 expression is also tightly regulated, and is found largely in the Pro-B through small Pre-B stages. Mb-1 is present from Pro-B throughout the pathway at high levels. Finally, Bcl-2 is expressed at high levels only at the pre-Pro-B and mature B stages, whereas it is low during all the intermediate stages. We also correlate this expression data with an analysis of the onset of Ig gene rearrangement as assessed by amplifying D-JH, VH-DJH, and VK-JK. Finally, we report differences in gene expression during B lymphopoiesis at two distinct ontogenic timings, in fetal liver and adult BM: both TdT and the precursor lymphocyte regulated myosin-like light chain are expressed at high levels in the Pro-B cell stage in bone marrow, but are absent from the corresponding fraction in fetal liver. In contrast, lambda 5, VpreB, Rag-1, and Rag-2 are expressed at comparable levels.
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Serra, P., Amrani, A., Han, B., Yamanouchi, J., Thiessen, S. J., Santamaria, P. (2002). RAG-dependent peripheral T cell receptor diversification in CD8+ T lymphocytes. Proc. Natl. Acad. Sci. USA 99: 15566-15571 [Abstract] [Full Text]
Meffre, E., Nussenzweig, M. C. (2002). Deletion of immunoglobulin beta in developing B cells leads to cell death. Proc. Natl. Acad. Sci. USA 99: 11334-11339 [Abstract] [Full Text]
Smith, E. M. K., Gisler, R., Sigvardsson, M. (2002). Cloning and Characterization of a Promoter Flanking the Early B Cell Factor (EBF) Gene Indicates Roles for E-Proteins and Autoregulation in the Control of EBF Expression. J. Immunol. 169: 261-270 [Abstract] [Full Text]
Gisler, R., Sigvardsson, M. (2002). The Human V-PreB Promoter Is a Target for Coordinated Activation by Early B Cell Factor and E47. J. Immunol. 168: 5130-5138 [Abstract] [Full Text]
Bichi, R., Shinton, S. A., Martin, E. S., Koval, A., Calin, G. A., Cesari, R., Russo, G., Hardy, R. R., Croce, C. M. (2002). Human chronic lymphocytic leukemia modeled in mouse by targeted TCL1 expression. Proc. Natl. Acad. Sci. USA 99: 6955-6960 [Abstract] [Full Text]
Rothstein, T. L. (2002). Two B-1 or Not To Be One. J. Immunol. 168: 4257-4261 [Abstract] [Full Text]
Chuang, P. I., Morefield, S., Liu, C.-Y., Chen, S., Harlan, J. M., Willerford, D. M. (2002). Perturbation of B-cell development in mice overexpressing the Bcl-2 homolog A1. Blood 99: 3350-3359 [Abstract] [Full Text]
Martinez-Barnetche, J., Madrid-Marina, V., Flavell, R. A., Moreno, J. (2002). Does CD40 Ligation Induce B Cell Negative Selection?. J. Immunol. 168: 1042-1049 [Abstract] [Full Text]
Douagi, I., Colucci, F., Di Santo, J. P., Cumano, A. (2002). Identification of the earliest prethymic bipotent T/NK progenitor in murine fetal liver. Blood 99: 463-471 [Abstract] [Full Text]
Cancro, M. P., Sah, A. P., Levy, S. L., Allman, D. M., Schmidt, M. R., Woodland, R. T. (2001). xid mice reveal the interplay of homeostasis and Bruton's tyrosine kinase-mediated selection at multiple stages of B cell development. Int Immunol 13: 1501-1514 [Abstract] [Full Text]
Kline, G. H., Hayden, T. A., Riegert, P. (2001). The Initiation of B Cell Clonal Expansion Occurs Independently of Pre-B Cell Receptor Formation. J. Immunol. 167: 5136-5142 [Abstract] [Full Text]
Martinez-M., J. A., Minguet, S., Gonzalo, P., Soro, P. G., de Andres, B., Izcue, A., Marcos, M. A. R., Gaspar, M.-L. (2001). Long-lived polyclonal B-cell lines derived from midgestation mouse embryo lymphohematopoietic progenitors reconstitute adult immunodeficient mice. Blood 98: 1862-1871 [Abstract] [Full Text]
Sieh, P., Chen, J. (2001). Distinct Control of the Frequency and Allelic Exclusion of the V{beta} Gene Rearrangement at the TCR{beta} Locus. J. Immunol. 167: 2121-2129 [Abstract] [Full Text]
Allman, D., Karnell, F. G., Punt, J. A., Bakkour, S., Xu, L., Myung, P., Koretzky, G. A., Pui, J. C., Aster, J. C., Pear, W. S. (2001). Separation of Notch1 Promoted Lineage Commitment and Expansion/Transformation in Developing T Cells. J. Exp. Med. 194: 99-106 [Abstract] [Full Text]
Sandel, P. C., Gendelman, M., Kelsoe, G., Monroe, J. G. (2001). Definition of a Novel Cellular Constituent of the Bone Marrow That Regulates the Response of Immature B Cells to B Cell Antigen Receptor Engagement. J. Immunol. 166: 5935-5944 [Abstract] [Full Text]
Jiang, A., Clark, E. A. (2001). Involvement of Bik, a Proapoptotic Member of the Bcl-2 Family, in Surface IgM-Mediated B Cell Apoptosis. J. Immunol. 166: 6025-6033 [Abstract] [Full Text]
Chiang, M. Y., Monroe, J. G. (2001). Role for Transcription Pax5A Factor in Maintaining Commitment to the B Cell Lineage by Selective Inhibition of Granulocyte-Macrophage Colony-Stimulating Factor Receptor Expression. J. Immunol. 166: 6091-6098 [Abstract] [Full Text]
Igarashi, H., Kuwata, N., Kiyota, K., Sumita, K., Suda, T., Ono, S., Bauer, S. R., Sakaguchi, N. (2001). Localization of recombination activating gene 1/green fluorescent protein (RAG1/GFP) expression in secondary lymphoid organs after immunization with T-dependent antigens in rag1/gfp knockin mice. Blood 97: 2680-2687 [Abstract] [Full Text]
Huang, D., Wang, J., Kivisakk, P., Rollins, B. J., Ransohoff, R. M. (2001). Absence of Monocyte Chemoattractant Protein 1 in Mice Leads to Decreased Local Macrophage Recruitment and Antigen-specific T Helper Cell Type 1 Immune Response in Experimental Autoimmune Encephalomyelitis. J. Exp. Med. 193: 713-726 [Abstract] [Full Text]
Mojica, M. P., Perry, S. S., Searles, A. E., Elenitoba-Johnson, K. S. J., Pierce, L. J., Wiesmann, A., Slayton, W. B., Spangrude, G. J. (2001). Phenotypic Distinction and Functional Characterization of Pro-B Cells in Adult Mouse Bone Marrow. J. Immunol. 166: 3042-3051 [Abstract] [Full Text]
Nitschke, L., Kestler, J., Tallone, T., Pelkonen, S., Pelkonen, J. (2001). Deletion of the DQ52 Element Within the Ig Heavy Chain Locus Leads to a Selective Reduction in VDJ Recombination and Altered D Gene Usage. J. Immunol. 166: 2540-2552 [Abstract] [Full Text]
Gartner, F., Alt, F. W., Monroe, R. J., Seidl, K. J. (2000). Antigen-independent Appearance of Recombination Activating Gene (RAG)-positive Bone Marrow B Cells in the Spleens of Immunized Mice. J. Exp. Med. 192: 1745-1754 [Abstract] [Full Text]
Litzenburger, T., Bluthmann, H., Morales, P., Pham-Dinh, D., Dautigny, A., Wekerle, H., Iglesias, A. (2000). Development of Myelin Oligodendrocyte Glycoprotein Autoreactive Transgenic B Lymphocytes: Receptor Editing In Vivo After Encounter of a Self-Antigen Distinct from Myelin Oligodendrocyte Glycoprotein. J. Immunol. 165: 5360-5366 [Abstract] [Full Text]
Klonowski, K. D., Monestier, M. (2000). Heavy Chain Revision in MRL Mice: A Potential Mechanism for the Development of Autoreactive B Cell Precursors. J. Immunol. 165: 4487-4493 [Abstract] [Full Text]
Maes, J., Caspi, Y., Rougeon, F., Haimovich, J., Goodhardt, M. (2000). Secondary V(D)J Rearrangements and B Cell Receptor-Mediated Down-Regulation of Recombination Activating Gene-2 Expression in a Murine B Cell Line. J. Immunol. 165: 703-709 [Abstract] [Full Text]
Nagaoka, H., Takahashi, Y., Hayashi, R., Nakamura, T., Ishii, K., Matsuda, J., Ogura, A., Shirakata, Y., Karasuyama, H., Sudo, T., Nishikawa, S.-I., Tsubata, T., Mizuochi, T., Asano, T., Sakano, H., Takemori, T. (2000). Ras Mediates Effector Pathways Responsible for Pre-B Cell Survival, Which Is Essential for the Developmental Progression to the Late Pre-B Cell Stage. J. Exp. Med. 192: 171-182 [Abstract] [Full Text]
LeBien, T. W. (2000). Fates of human B-cell precursors. Blood 96: 9-23 [Abstract] [Full Text]
Nagaoka, H., Gonzalez-Aseguinolaza, G., Tsuji, M., Nussenzweig, M. C. (2000). Immunization and Infection Change the Number of Recombination Activating Gene (RAG)-expressing B Cells in the Periphery by Altering Immature Lymphocyte Production. J. Exp. Med. 191: 2113-2120 [Abstract] [Full Text]
Shinall, S. M., Gonzalez-Fernandez, M., Noelle, R. J., Waldschmidt, T. J. (2000). Identification of Murine Germinal Center B Cell Subsets Defined by the Expression of Surface Isotypes and Differentiation Antigens. J. Immunol. 164: 5729-5738 [Abstract] [Full Text]
Chumley, M. J., Dal Porto, J. M., Kawaguchi, S., Cambier, J. C., Nemazee, D., Hardy2, R. R. (2000). A VH11V{kappa}9 B Cell Antigen Receptor Drives Generation of CD5+ B Cells Both In Vivo and In Vitro. J. Immunol. 164: 4586-4593 [Abstract] [Full Text]
Geiman, T. M., Muegge, K. (2000). Lsh, an SNF2/helicase family member, is required for proliferation of mature T lymphocytes. Proc. Natl. Acad. Sci. USA 97: 4772-4777 [Abstract] [Full Text]
Murante, F. G., Gasiewicz, T. A. (2000). Hemopoietic Progenitor Cells Are Sensitive Targets of 2,3,7,8-Tetrachlorodibenzo-p-dioxin in C57BL/6J Mice. Toxicol Sci 54: 374-383 [Abstract] [Full Text]
Medina, K. L., Strasser, A., Kincade, P. W. (2000). Estrogen influences the differentiation, proliferation, and survival of early B-lineage precursors. Blood 95: 2059-2067 [Abstract] [Full Text]
Ogawa, M., Boekel, E. t., Melchers, F. (2000). Identification of CD19-B220+c-Kit+Flt3/Flk-2+cells as early B lymphoid precursors before pre-B-I cells in juvenile mouse bone marrow. Int Immunol 12: 313-324 [Abstract] [Full Text]
Mandik-Nayak, L., Nayak, S., Sokol, C., Eaton-Bassiri, A., Madaio, M. P., Caton, A. J., Erikson, J. (2000). The origin of anti-nuclear antibodies in bcl-2 transgenic mice. Int Immunol 12: 353-364 [Abstract] [Full Text]
Brightbill, H. D., Plevy, S. E., Modlin, R. L., Smale, S. T. (2000). A Prominent Role for Sp1 During Lipopolysaccharide- Mediated Induction of the IL-10 Promoter in Macrophages. J. Immunol. 164: 1940-1951 [Abstract] [Full Text]
Wei, C., Zeff, R., Goldschneider, I. (2000). Murine Pro-B Cells Require IL-7 and Its Receptor Complex to Up-Regulate IL-7R{alpha}, Terminal Deoxynucleotidyltransferase, and c{micro} Expression. J. Immunol. 164: 1961-1970 [Abstract] [Full Text]
Moreau, M.-F., Thibaud, J.-L., Miled, L. B., Chaussepied, M., Baumgartner, M., Davis, W. C., Minoprio, P., Langsley, G. (1999). Theileria annulata in CD5+ Macrophages and B1 B Cells. Infect. Immun. 67: 6678-6682 [Abstract] [Full Text]
Kim, D., Peng, X.-C., Sun, X.-H. (1999). Massive Apoptosis of Thymocytes in T-Cell-Deficient Id1 Transgenic Mice. Mol. Cell. Biol. 19: 8240-8253 [Abstract] [Full Text]
Ohmura, K., Kawamoto, H., Fujimoto, S., Ozaki, S., Nakao, K., Katsura, Y. (1999). Emergence of T, B, and Myeloid Lineage-Committed as well as Multipotent Hemopoietic Progenitors in the Aorta-Gonad- Mesonephros Region of Day 10 Fetuses of the Mouse. J. Immunol. 163: 4788-4795 [Abstract] [Full Text]
Monroe, R. J., Chen, F., Ferrini, R., Davidson, L., Alt, F. W. (1999). RAG2 is regulated differentially in B and T cells by elements 5' of the promoter. Proc. Natl. Acad. Sci. USA 96: 12713-12718 [Abstract] [Full Text]
Bosma, G. C., Chang, Y., Karasuyama, H., Bosma, M. J. (1999). Differential effect of an Ig {micro} transgene on development of pre-B cells in fetal and adult SCID mice. Proc. Natl. Acad. Sci. USA 96: 11952-11957 [Abstract] [Full Text]
Benschop, R. J., Melamed, D., Nemazee, D., Cambier, J. C. (1999). Distinct Signal Thresholds for the Unique Antigen Receptor�linked Gene Expression Programs in Mature and Immature B Cells. J. Exp. Med. 190: 749-756 [Abstract] [Full Text]
Morrow, T. A., Muljo, S. A., Zhang, J., Hardwick, J. M., Schlissel, M. S. (1999). Pro-B-Cell-Specific Transcription and Proapoptotic Function of Protein Kinase Ceta. Mol. Cell. Biol. 19: 5608-5618 [Abstract] [Full Text]
Ruetsch, N. R., Bosma, G. C., Bosma, M. J. (1999). Unexpected Rearrangement and Expression of the Immunoglobulin {lambda}1 Locus in Scid Mice. J. Exp. Med. 191: 1933-1944 [Abstract] [Full Text]
Popov, A. V., Zou, X., Xian, J., Nicholson, I. C., Bruggemann, M. (1999). A Human Immunoglobulin lambda �Locus Is Similarly Well Expressed in Mice and Humans. J. Exp. Med. 189: 1611-1620 [Abstract] [Full Text]
Weinmann, P., Gaehtgens, P., Walzog, B. (1999). Bcl-Xl- and Bax-alpha -Mediated Regulation of Apoptosis of Human Neutrophils Via Caspase-3. Blood 93: 3106-3115 [Abstract] [Full Text]
Chang, Y., Bosma, M. J., Bosma, G. C. (1999). Extended Duration of DH-JH Rearrangement in Immunoglobulin Heavy Chain Transgenic Mice: Implications for Regulation of Allelic Exclusion. J. Exp. Med. 189: 1295-1305 [Abstract] [Full Text]
Cariappa, A., Kim, T. J., Pillai, S. (1999). Accelerated Emigration of B Lymphocytes in the Xid Mouse. J. Immunol. 162: 4417-4423 [Abstract] [Full Text]
Sikes, M. L., Suarez, C. C., Oltz, E. M. (1999). Regulation of V(D)J Recombination by Transcriptional Promoters. Mol. Cell. Biol. 19: 2773-2781 [Abstract] [Full Text]
Shaw, A. C., Swat, W., Davidson, L., Alt, F. W. (1999). Induction of Ig light chain gene rearrangement in heavy chain-deficient B cells by activated Ras. Proc. Natl. Acad. Sci. USA 96: 2239-2243 [Abstract] [Full Text]
Kawamoto, H., Ohmura, K., Fujimoto, S., Katsura, Y. (1999). Emergence of T Cell Progenitors Without B Cell or Myeloid Differentiation Potential at the Earliest Stage of Hematopoiesis in the Murine Fetal Liver. J. Immunol. 162: 2725-2731 [Abstract] [Full Text]
Tomasson, M. H., Williams, I. R., Hasserjian, R., Udomsakdi, C., McGrath, S. M., Schwaller, J., Druker, B., Gilliland, D. G. (1999). TEL/PDGFbeta R Induces Hematologic Malignancies in Mice That Respond to a Specific Tyrosine Kinase Inhibitor. Blood 93: 1707-1714 [Abstract] [Full Text]