Recognition and destruction of Bacillus Calmette-Guerin-infected human monocytes

doi:10.1084/jem.177.6.1691

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Recognition and destruction of Bacillus Calmette-Guerin-infected human monocytes

A Molloy, PA Meyn, KD Smith and G Kaplan
Department of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021.

We have established a long-term culture system to study macrophages chronically infected with mycobacteria. Monocytes are infected with Bacillus Calmette-Guerin (BCG) and support exponential intracellular replication without profound perturbation of normal host cell function. We have used this system to investigate lymphokine-activated killer (LAK)-mediated cytolysis. We have found that interleukin 2 stimulation of peripheral blood lymphocytes generates a cytotoxic activity against human monocytes. A CD56- subpopulation of LAK cells specifically recognizes and lyses BCG-infected cells. Lysis of the host cell has no effect on parasite viability and results in the liberation of bacteria capable of infecting more cells.
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