Functional neurokinin 1 receptors for substance P are expressed by human vascular endothelium

doi:10.1084/jem.177.5.1269

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Greeno, E. W.
	Articles by Moldow, C. F.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Greeno, E. W.
	Articles by Moldow, C. F.


Social Bookmarking

	What's this?

ARTICLES

Functional neurokinin 1 receptors for substance P are expressed by human vascular endothelium

EW Greeno, P Mantyh, GM Vercellotti and CF Moldow
Department of Medicine, University of Minnesota, Minneapolis.

Substance P (SP), a neurotachykinin, is important in a number of inflammatory processes in which the endothelial cell also plays a critical role. SP receptors have previously been identified only on arterial endothelium, and the scant in vitro evidence for direct effects of SP on human endothelium is based on studies using nonarterial cells. To better understand SP's role in inflammation, we sought to identify functional SP receptors on human endothelium in situ and in culture. Autoradiographic ligand binding to human umbilical cord sections demonstrates the presence of SP binding sites with characteristics of the neurokinin 1 (NK-1) receptor (displacement by GTP analogues and the NK-1 specific antagonist CP-96,345) on human umbilical arterial, but not venous, endothelium. In culture, human umbilical venous endothelial cells (HUVECs) and human aortic endothelial cells express low levels of available SP binding sites. However, HUVECs, which are serum starved and refed, undergo a dramatic increase in SP binding. SP binding to starved/refed HUVECs induces a transient increase in intracellular calcium. This calcium flux is dose dependent over appropriate SP concentrations and can be blocked by NK-1 specific antagonists. The proinflammatory effects of SP may be mediated in part through the NK-1 receptor on endothelium.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Nowicki, M., Ostalska-Nowicka, D., Kondraciuk, B., Miskowiak, B. (2007). The significance of substance P in physiological and malignant haematopoiesis. J. Clin. Pathol. 60: 749-755 [Abstract] [Full Text]
Pal, S., Wu, J., Murray, J. K., Gellman, S. H., Wozniak, M. A., Keely, P. J., Boyer, M. E., Gomez, T. M., Hasso, S. M., Fallon, J. F., Bresnick, E. H. (2006). An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis. J. Cell Biol. 174: 1047-1058 [Abstract] [Full Text]
Pal, S., Nemeth, M. J., Bodine, D., Miller, J. L., Svaren, J., Thein, S. L., Lowry, P. J., Bresnick, E. H. (2004). Neurokinin-B Transcription in Erythroid Cells: DIRECT ACTIVATION BY THE HEMATOPOIETIC TRANSCRIPTION FACTOR GATA-1. J. Biol. Chem. 279: 31348-31356 [Abstract] [Full Text]
Weinstock, J. V., Blum, A., Metwali, A., Elliott, D., Bunnett, N., Arsenescu, R. (2003). Substance P Regulates Th1-Type Colitis in IL-10 Knockout Mice. J. Immunol. 171: 3762-3767 [Abstract] [Full Text]
Weinstock, J. V., Blum, A., Metwali, A., Elliott, D., Arsenescu, R. (2003). IL-18 and IL-12 Signal Through the NF-{kappa}B Pathway to Induce NK-1R Expression on T Cells. J. Immunol. 170: 5003-5007 [Abstract] [Full Text]
Blum, A. M., Metwali, A., Elliott, D. E., Weinstock, J. V. (2003). T cell substance P receptor governs antigen-elicited IFN-gamma production. Am. J. Physiol. Gastrointest. Liver Physiol. 284: G197-G204 [Abstract] [Full Text]
Wong, S. S, Sun, N. N, Lantz, R C., Witten, M. L (2002). Tissue-specific patterns of neurokinin-1 receptor (NK-1R) gene expression in mice exposed to sidestream cigarette smoke. Toxicol Ind Health 18: 435-444 [Abstract]
Nathan, J. D., Peng, R. Y., Wang, Y., McVey, D. C., Vigna, S. R., Liddle, R. A. (2002). Primary sensory neurons: a common final pathway for inflammation in experimental pancreatitis in rats. Am. J. Physiol. Gastrointest. Liver Physiol. 283: G938-G946 [Abstract] [Full Text]
BLUM, A. M., METWALI, A., CRAWFORD, C., LI, J., QADIR, K., ELLIOTT, D. E., WEINSTOCK, J. V. (2001). Interleukin 12 and antigen independently induce substance P receptor expression in T cells in murine schistosomiasis mansoni. FASEB J. 15: 950-957 [Abstract] [Full Text]
Goode, T, O'Connell, J, Anton, P, Wong, H, Reeve, J, O'Sullivan, G C, Collins, J K, Shanahan, F (2000). Neurokinin-1 receptor expression in inflammatory bowel disease: molecular quantitation and localisation. Gut 47: 387-396 [Abstract] [Full Text]
Hammond, T. G., Saban, R., Bost, K. L., Harris, H. W. Jr., Kaysen, J. H., Goda, F. O., Wang, X.-C., Lewis, F. C., Navar, G. L., Campbell, W. C., Bjorling, D. E., Saban, M., Zeidel, M. L. (2000). Substance P dependence of endosomal fusion during bladder inflammation. Am. J. Physiol. Renal Physiol. 278: F440-F451 [Abstract] [Full Text]
Schaffer, M., Beiter, T., Becker, H. D., Hunt, T. K. (1998). Neuropeptides: Mediators of Inflammation and Tissue Repair?. Arch Surg 133: 1107-1116 [Full Text]