Elevated secretion of reactive nitrogen and oxygen intermediates by inflammatory leukocytes in hyperacute experimental autoimmune encephalomyelitis: enhancement by the soluble products of encephalitogenic T cells

doi:10.1084/jem.176.1.303

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by MacMicking, J. D.
	Articles by Cowden, W. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by MacMicking, J. D.
	Articles by Cowden, W. B.


Social Bookmarking

	What's this?

ARTICLES

Elevated secretion of reactive nitrogen and oxygen intermediates by inflammatory leukocytes in hyperacute experimental autoimmune encephalomyelitis: enhancement by the soluble products of encephalitogenic T cells

JD MacMicking, DO Willenborg, MJ Weidemann, KA Rockett and WB Cowden
Division of Cell Biology, John Curtin School of Medical Research, Canberra, Australia.

Perivascular lesions within the central nervous system (CNS) of rats with hyperacute experimental autoimmune encephalomyelitis (HEAE) contained large numbers of peripheral blood mononuclear cells (PBMC) and polymorphonuclear leukocytes (PMN), cells enzymatically capable of producing reactive nitrogen and oxygen intermediates (RNI and ROI), which, in excess, are mediators of tissue damage. PBMC and PMN isolated from the CNS and periphery of HEAE-affected rats secreted significantly (p less than 0.01-0.0001) elevated levels of ROI and RNI compared with that of similar cell populations from pertussis- and saline-treated control animals. Coincubation of systemically derived PBMC and PMN with antigen-stimulated myelin basic protein-specific T cell lines led to further increases in ROI and RNI output of between 15.3 and 83.1%, an effect that could be largely attributed to heat-labile, soluble products released by these T cell lines. Our studies suggest a putative neuropathological role for ROI and RNI in HEAE, which may be mediated via cytokines emanating from autoreactive T lymphocytes.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Hirotani, M, Maita, C, Niino, M, Iguchi-Ariga, S., Hamada, S, Ariga, H, Sasaki, H (2008). Correlation between DJ-1 levels in the cerebrospinal fluid and the progression of disabilities in multiple sclerosis patients. Mult Scler 14: 1056-1060 [Abstract]
Zehntner, S. P., Brickman, C., Bourbonniere, L., Remington, L., Caruso, M., Owens, T. (2005). Neutrophils That Infiltrate the Central Nervous System Regulate T Cell Responses. J. Immunol. 174: 5124-5131 [Abstract] [Full Text]
Staykova, M. A., Paridaen, J. T., Cowden, W. B., Willenborg, D. O. (2005). Nitric Oxide Contributes to Resistance of the Brown Norway Rat to Experimental Autoimmune Encephalomyelitis. Am. J. Pathol. 166: 147-157 [Abstract] [Full Text]
Aktas, O., Prozorovski, T., Smorodchenko, A., Savaskan, N. E., Lauster, R., Kloetzel, P.-M., Infante-Duarte, C., Brocke, S., Zipp, F. (2004). Green Tea Epigallocatechin-3-Gallate Mediates T Cellular NF-{kappa}B Inhibition and Exerts Neuroprotection in Autoimmune Encephalomyelitis. J. Immunol. 173: 5794-5800 [Abstract] [Full Text]
Kanwar, J. R., Kanwar, R. K., Krissansen, G. W. (2004). Simultaneous neuroprotection and blockade of inflammation reverses autoimmune encephalomyelitis. Brain 127: 1313-1331 [Abstract] [Full Text]
Ni, X., Geller, E. B, Eppihimer, M. J, Eisenstein, T. K, Adler, M. W, Tuma, R. F (2004). Win 55212-2, a cannabinoid receptor agonist, attenuates leukocyte/endothelial interactions in an experimental autoimmune encephalomyelitis model. Mult Scler 10: 158-164 [Abstract]
Chakrabarty, A, Emerson, M R, LeVine, S M (2003). Hemeoxygenase-1 in SJL mice with experimental allergic encephalomyelitis. Mult Scler 9: 372-381 [Abstract]
Suliman, H. B., Ryan, L. K., Bishop, L., Folz, R. J. (2001). Prevention of influenza-induced lung injury in mice overexpressing extracellular superoxide dismutase. Am. J. Physiol. Lung Cell. Mol. Physiol. 280: L69-L78 [Abstract] [Full Text]
Cho, D., Song, H., Kim, Y. M., Houh, D., Hur, D. Y., Park, H., Yoon, D., Pyun, K. H., Lee, W. J., Kurimoto, M., Kim, Y. B., Kim, Y. S., Choi, I. (2000). Endogenous Interleukin-18 Modulates Immune Escape of Murine Melanoma Cells by Regulating the Expression of Fas Ligand and Reactive Oxygen Intermediates. Cancer Res. 60: 2703-2709 [Abstract] [Full Text]
O'Brien, N. C., Charlton, B., Cowden, W. B., Willenborg, D. O. (1999). Nitric Oxide Plays a Critical Role in the Recovery of Lewis Rats from Experimental Autoimmune Encephalomyelitis and the Maintenance of Resistance to Reinduction. J. Immunol. 163: 6841-6847 [Abstract] [Full Text]
Vartanian, T. K., de la Monte, S. (1999). Case 1-1999- A 53-Year-Old Man with Fever and Rapid Neurologic Deterioration. NEJM 340: 127-135 [Full Text]
Karupiah, G., Chen, J.-H., Mahalingam, S., Nathan, C. F., MacMicking, J. D. (1998). Rapid Interferon gamma -dependent Clearance of Influenza A Virus and Protection from Consolidating Pneumonitis in Nitric Oxide Synthase 2-deficient Mice. J. Exp. Med. 188: 1541-1546 [Abstract] [Full Text]
Vladimirova, O., O'Connor, J., Cahill, A., Alder, H., Butunoi, C., Kalman, B. (1998). Oxidative damage to DNA in plaques of MS brains. Mult Scler 4: 413-418 [Abstract]
Fenyk-Melody, J. E., Garrison, A. E., Brunnert, S. R., Weidner, J. R., Shen, F., Shelton, B. A., Mudgett, J. S. (1998). Experimental Autoimmune Encephalomyelitis Is Exacerbated in Mice Lacking the NOS2 Gene. J. Immunol. 160: 2940-2946 [Abstract] [Full Text]