Murine thymic CD4+ T cell subsets: a subset (Thy0) that secretes diverse cytokines and overexpresses the V beta 8 T cell receptor gene family

doi:10.1084/jem.176.1.269

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Hayakawa, K.
	Articles by Hardy, R. R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Hayakawa, K.
	Articles by Hardy, R. R.


Social Bookmarking

	What's this?

ARTICLES

Murine thymic CD4+ T cell subsets: a subset (Thy0) that secretes diverse cytokines and overexpresses the V beta 8 T cell receptor gene family

K Hayakawa, BT Lin and RR Hardy
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.

We demonstrate here the presence of a distinct mature CD4+8- T cell subset in mouse thymus. This subset, termed "Thy0," is delineated by the absence of 3G11 expression from about half of the 6C10-/HSAlow/- fraction of CD4+8- thymic cells. Thy0 is detectable from the neonatal period and largely contributes the Th0-type diverse cytokine production previously reported for the HSAlow/-CD4+ thymic population. Further, cells expressing the T cell receptor V beta 8 gene family are found at increasing frequency in Thy0 with age, comprising 40-60% of Thy0 in adult BALB/c mice. This alteration of V beta 8+ cell frequency is unique to Thy0, since no other CD4+ subset in thymus or spleen shows such V beta 8 overusage. All functional CD4+ T cell subsets, including Thy0, show appropriate V beta clonal deletion associated with endogenous superantigens. Thus, it appears that Thy0 is an intrathymically generated secondary cell subset produced after CD4+ T cell selection.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Gadue, P., Yin, L., Jain, S., Stein, P. L. (2004). Restoration of NK T Cell Development in fyn-Mutant Mice by a TCR Reveals a Requirement for Fyn During Early NK T Cell Ontogeny. J. Immunol. 172: 6093-6100 [Abstract] [Full Text]
Cernadas, M., Sugita, M., van der Wel, N., Cao, X., Gumperz, J. E., Maltsev, S., Besra, G. S., Behar, S. M., Peters, P. J., Brenner, M. B. (2003). Lysosomal Localization of Murine CD1d Mediated by AP-3 Is Necessary for NK T Cell Development. J. Immunol. 171: 4149-4155 [Abstract] [Full Text]
Inui, T., Nakagawa, R., Ohkura, S., Habu, Y., Koike, Y., Motoki, K., Kuranaga, N., Fukasawa, M., Shinomiya, N., Seki, S. (2002). Age-Associated Augmentation of the Synthetic Ligand- Mediated Function of Mouse NK1.1 Ag+ T Cells: Their Cytokine Production and Hepatotoxicity In Vivo and In Vitro. J. Immunol. 169: 6127-6132 [Abstract] [Full Text]
Gadue, P., Stein, P. L. (2002). NK T Cell Precursors Exhibit Differential Cytokine Regulation and Require Itk for Efficient Maturation. J. Immunol. 169: 2397-2406 [Abstract] [Full Text]
Gui, M., Li, J., Wen, L.-J., Hardy, R. R., Hayakawa, K. (2001). TCR{beta} Chain Influences But Does Not Solely Control Autoreactivity of V{alpha}14J281T Cells. J. Immunol. 167: 6239-6246 [Abstract] [Full Text]
Baur, N., Nerz, G., Nil, A., Eichmann, K. (2001). Expression and selection of productively rearranged TCR{beta} VDJ genes are sequentially regulated by CD3 signaling in the development of NK1.1+ {alpha}{beta} T cells. Int Immunol 13: 1031-1042 [Abstract] [Full Text]
Hammond, K. J. L., Pellicci, D. G., Poulton, L. D., Naidenko, O. V., Scalzo, A. A., Baxter, A. G., Godfrey, D. I. (2001). CD1d-Restricted NKT Cells: An Interstrain Comparison. J. Immunol. 167: 1164-1173 [Abstract] [Full Text]
Araujo, L. M., Puel, A., Gouarin, C., Hameg, A., Mevel, J.-C., Koezuka, Y., Bach, J.-F., Mouton, D., Herbelin, A. (2000). NKT lymphocyte ontogeny and function are impaired in low antibody-producer Biozzi mice: gene mapping in the interval-specific congenic strains raised for immunomodulatory genes. Int Immunol 12: 1613-1622 [Abstract] [Full Text]
Viret, C., Lantz, O., He, X., Bendelac, A., Janeway, C. A. Jr. (2000). A NK1.1+ Thymocyte-Derived TCR {beta}-Chain Transgene Promotes Positive Selection of Thymic NK1.1+ {alpha}{beta} T Cells. J. Immunol. 165: 3004-3014 [Abstract] [Full Text]
Takayama, E., Seki, S., Ohkawa, T., Ami, K., Habu, Y., Yamaguchi, T., Tadakuma, T., Hiraide, H. (2000). Mouse CD8+ CD122+ T Cells with Intermediate TCR Increasing with Age Provide a Source of Early IFN-{gamma} Production. J. Immunol. 164: 5652-5658 [Abstract] [Full Text]
Walunas, T. L., Wang, B., Wang, C.-R., Leiden, J. M. (2000). Cutting Edge: The Ets1 Transcription Factor Is Required for the Development of NK T Cells in Mice. J. Immunol. 164: 2857-2860 [Abstract] [Full Text]
Salmon, P., Mong, M., Kang, X.-J., Cado, D., Robey, E. (1999). The Role of CD8{alpha}' in the CD4 Versus CD8 Lineage Choice. J. Immunol. 163: 5312-5318 [Abstract] [Full Text]
Chen, Y.-T., Chen, F.-L., Kung, J. T. (1999). Age-Associated Rapid and Stat6-Independent IL-4 Production by NK1-CD4+8- Thymus T Lymphocytes. J. Immunol. 163: 4747-4753 [Abstract] [Full Text]
Gadue, P., Morton, N., Stein, P. L. (1999). The Src Family Tyrosine Kinase Fyn Regulates Natural Killer T Cell Development. J. Exp. Med. 190: 1189-1196 [Abstract] [Full Text]
Gerber, D. J., Azuara, V., Levraud, J.-P., Huang, S. Y., Lembezat, M.-P., Pereira, P. (1999). IL-4-Producing {gamma}{delta} T Cells That Express a Very Restricted TCR Repertoire Are Preferentially Localized in Liver and Spleen. J. Immunol. 163: 3076-3082 [Abstract] [Full Text]
Naiki, Y., Nishimura, H., Kawano, T., Tanaka, Y., Itohara, S., Taniguchi, M., Yoshikai, Y. (1999). Regulatory Role of Peritoneal NK1.1+{alpha}{beta} T Cells in IL-12 Production During Salmonella Infection. J. Immunol. 163: 2057-2063 [Abstract] [Full Text]
Chen, Y.-H., Wang, B., Chun, T., Zhao, L., Cardell, S., Behar, S. M., Brenner, M. B., Wang, C.-R. (1999). Expression of CD1d2 on Thymocytes Is Not Sufficient for the Development of NK T Cells in CD1d1-Deficient Mice. J. Immunol. 162: 4560-4566 [Abstract] [Full Text]
Andersson, A., Dai, W. J., Di Santo, J. P., Brombacher, F. (1998). Early IFN-{gamma} Production and Innate Immunity During Listeria monocytogenes Infection in the Absence of NK Cells. J. Immunol. 161: 5600-5606 [Abstract] [Full Text]
Herbelin, A., Gombert, J.-M., Lepault, F., Bach, J.-F., Chatenoud, L. (1998). Mature Mainstream TCR{alpha}{beta}+CD4+ Thymocytes Expressing L-Selectin Mediate ""Active Tolerance"" in the Nonobese Diabetic Mouse. J. Immunol. 161: 2620-2628 [Abstract] [Full Text]
Morris, S. C., Coffman, R. L., Finkelman, F. D. (1998). In Vivo IL-4 Responses to Anti-IgD Antibody Are MHC Class II Dependent and {beta}2-Microglobulin Independent and Develop Normally in the Absence of IL-4 Priming of T Cells. J. Immunol. 160: 3299-3304 [Abstract] [Full Text]
Exley, M., Garcia, J., Balk, S. P., Porcelli, S. (1997). Requirements for CD1d Recognition by Human Invariant Valpha 24+ CD4-CD8- T Cells. J. Exp. Med. 186: 109-120 [Abstract] [Full Text]
Lantz, O., Sharara, L. I., Tilloy, F., Andersson, A., DiSanto, J. P. (1997). Lineage Relationships and Differentiation of Natural Killer (NK) T Cells: Intrathymic Selection and Interleukin (IL)-4 Production in the Absence of NKR-P1 and Ly49 Molecules. J. Exp. Med. 185: 1395-1402 [Abstract] [Full Text]
Yoshimoto, T., Bendelac, A., Watson, C., Hu-Li, J., Paul, W. E. (1995). Role of NK1.1+ T Cells in a TH2 Response and in Immunoglobulin E Production. Science 270: 1845-1847 [Abstract]
Bendelac, A (1995). CD1: presenting unusual antigens to unusual T lymphocytes. Science 269: 185-186
Bendelac, A, Lantz, O, Quimby, M., Yewdell, J., Bennink, JR, Brutkiewicz, R. (1995). CD1 recognition by mouse NK1+ T lymphocytes. Science 268: 863-865 [Abstract]
Bendelac, A, Killeen, N, Littman, D., Schwartz, R. (1994). A subset of CD4+ thymocytes selected by MHC class I molecules. Science 263: 1774-1778 [Abstract]
van Meerwijk, J., Germain, R. (1993). Development of mature CD8+ thymocytes: selection rather than instruction?. Science 261: 911-915 [Abstract]