Measles virus transmembrane fusion protein synthesized de novo or presented in immunostimulating complexes is endogenously processed for HLA class I- and class II-restricted cytotoxic T cell recognition

doi:10.1084/jem.176.1.119

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by van Binnendijk, R. S.
	Articles by UytdeHaag, F. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by van Binnendijk, R. S.
	Articles by UytdeHaag, F. G.


Social Bookmarking

	What's this?

ARTICLES

Measles virus transmembrane fusion protein synthesized de novo or presented in immunostimulating complexes is endogenously processed for HLA class I- and class II-restricted cytotoxic T cell recognition

RS van Binnendijk, CA van Baalen, MC Poelen, P de Vries, J Boes, V Cerundolo, AD Osterhaus and FG UytdeHaag
Laboratory of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.

The routes used by antigen-presenting cells (APC) to convert the transmembrane fusion glycoprotein (F) of measles virus (MV) to HLA class I and class II presentable peptides have been examined, using cloned cytotoxic T lymphocytes in functional assays. Presentation by Epstein-Barr virus-transformed B lymphoblastoid cell lines was achieved using live virus, ultraviolet light-inactivated virus, and purified MV- F delivered either as such or incorporated in immunostimulating complexes (MV-F-ISCOM). Only live virus and MV-F-ISCOM allow presentation by class I molecules, while all antigen preparations permit class II-restricted presentation. We observe presentation of MV- F from live virus and as MV-F-ISCOM by class II molecules in a fashion that is not perturbed by chloroquine. Our studies visualize novel presentation pathways of type I transmembrane proteins.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Arguelles, M. H., Orellana, M. L., Castello, A. A., Villegas, G. A., Masini, M., Belizan, A. L., Gonzalez Ayala, S., Vera, O. D., Glikmann, G. (2006). Measles virus-specific antibody levels in individuals in Argentina who received a one-dose vaccine.. J. Clin. Microbiol. 44: 2733-2738 [Abstract] [Full Text]
Beacock-Sharp, H., Donachie, A. M., Robson, N. C., Mowat, A. M. (2003). A role for dendritic cells in the priming of antigen-specific CD4+ and CD8+ T lymphocytes by immune-stimulating complexes in vivo. Int Immunol 15: 711-720 [Abstract] [Full Text]
Herberts, C. A., Stittelaar, K. J., van der Heeft, E., van Gaans-van den Brink, J., Poelen, M. C. M., Roholl, P. J. M., van Alphen, L. J. W., Melief, C. J. M., de Jong, A. P. J. M., van Els, C. A. C. M. (2001). A measles virus glycoprotein-derived human CTL epitope is abundantly presented via the proteasomal-dependent MHC class I processing pathway. J. Gen. Virol. 82: 2131-2142 [Abstract] [Full Text]
Valmori, D., Levy, F., Miconnet, I., Zajac, P., Spagnoli, G. C., Rimoldi, D., Lienard, D., Cerundolo, V., Cerottini, J.-C., Romero, P. (2000). Induction of Potent Antitumor CTL Responses by Recombinant Vaccinia Encoding a Melan-A Peptide Analogue. J. Immunol. 164: 1125-1131 [Abstract] [Full Text]
Kurlander, R. J., Chao, E., Fields, J., Nataraj, C. (1999). The Adjacent Flanking Region Plays a Critical Role in Facilitating the Presentation of the Listeria monocytogenes Product lemA to H2 M3wt-Restricted, Peptide-Specific Murine CD8 Cells. J. Immunol. 163: 6741-6747 [Abstract] [Full Text]
Gromme, M., Uytdehaag, F. G. C. M., Janssen, H., Calafat, J., van Binnendijk, R. S., Kenter, M. J. H., Tulp, A., Verwoerd, D., Neefjes, J. (1999). Recycling MHC class I molecules and endosomal peptide loading. Proc. Natl. Acad. Sci. USA 96: 10326-10331 [Abstract] [Full Text]
Luckey, C. J., King, G. M., Marto, J. A., Venketeswaran, S., Maier, B. F., Crotzer, V. L., Colella, T. A., Shabanowitz, J., Hunt, D. F., Engelhard, V. H. (1998). Proteasomes Can Either Generate or Destroy MHC Class I Epitopes: Evidence for Nonproteasomal Epitope Generation in the Cytosol. J. Immunol. 161: 112-121 [Abstract] [Full Text]
Bouche, F., Ammerlaan, W., Berthet, F., Houard, S., Schneider, F., Muller, C. P. (1998). Immunosorbent Assay Based on Recombinant Hemagglutinin Protein Produced in a High-Efficiency Mammalian Expression System for Surveillance of Measles Immunity. J. Clin. Microbiol. 36: 721-726 [Abstract] [Full Text]
Mosse, C. A., Meadows, L., Luckey, C. J., Kittlesen, D. J., Huczko, E. L., Slingluff, C. L. Jr., Shabanowitz, J., Hunt, D. F., Engelhard, V. H. (1998). The Class I Antigen-processing Pathway for the Membrane Protein Tyrosinase Involves Translation in the Endoplasmic Reticulum and Processing in the Cytosol. J. Exp. Med. 187: 37-48 [Abstract] [Full Text]
Lanzavecchia, A (1993). Identifying strategies for immune intervention. Science 260: 937-944 [Abstract]