Cloning and expression of class I major histocompatibility complex genes of the rat

doi:10.1084/jem.175.6.1749

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Cloning and expression of class I major histocompatibility complex genes of the rat

SC Jameson, WD Tope, EM Tredgett, JM Windle, AG Diamond and JC Howard
Department of Immunology, Agricultural and Food Research Council Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, England.

Little is known about the organization of class I genes in the rat although there is prima facie evidence that it is distinct from that of the mouse. We report the cloning of 61 nonclassical rat class I genes into cosmid clusters with a total mapped length of 1,264 kb. It is certain that the total number of class I genes in the rat must exceed this number. From restriction maps it is possible to identify substantial regions of duplication. By transfection of cosmids into mouse L cells, it has been possible to demonstrate at least seven different nonclassical rat class I genes that are expressible on the cell surface. Crossreaction of a single mouse monoclonal antibody with all of these class I molecules is consistent with sequence homogenization within the rat nonclassical system. Attempts to find rat homologues of the mouse Tla genes by crosshybridization of rat cosmids with a range of different TLa-specific probes were unsuccessful, suggesting that this large group of divergent class I genes is absent or nearly so from the rat. The large number of class I genes in the rat appears to have arisen by expansion of genes more closely related to the classical sequence.
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