Interleukin 10 and interferon gamma regulation of experimental Trypanosoma cruzi infection

doi:10.1084/jem.175.1.169

This Article

	Full Text (PDF, 740K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Silva, J. S.
	Articles by Reed, S. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Silva, J. S.
	Articles by Reed, S. G.


Social Bookmarking

	What's this?

ARTICLES

Interleukin 10 and interferon gamma regulation of experimental Trypanosoma cruzi infection

JS Silva, PJ Morrissey, KH Grabstein, KM Mohler, D Anderson and SG Reed
Seattle Biomedical Research Institute, Washington 98109.

Studies were undertaken to determine whether interleukin 10, (IL-10) a cytokine shown to inhibit interferon gamma (IFN-gamma) production, was involved in Trypanosoma cruzi infections in mice. Exogenous IFN-gamma protects mice from fatal infection with T. cruzi. Furthermore, resistant B6D2 mice developed fatal T. cruzi infections when treated with neutralizing anti-IFN-gamma monoclonal antibody (mAb). Thus, endogenous as well as exogenous IFN-gamma is important in mediating resistance to this parasite. Because both T. cruzi-susceptible (B6) and -resistant (B6D2) mouse strains produced IFN-gamma during acute infection, we looked for the concomitant production of mediators that could interfere with IFN-gamma-mediated resistance to T. cruzi. We found that IL-10-specific mRNA was produced in the spleens of mice with acute T. cruzi infections. In addition, spleen cell culture supernatants from infected B6 mice, and to a lesser extent B6D2 mice, elaborated an inhibitor(s) of IFN-gamma production. This inhibitor(s) was neutralized by anti-IL-10 mAb. These experiments demonstrated the production of biologically active IL-10 during T. cruzi infection. In further studies in vitro, it was shown that IL-10 blocked the ability of IFN-gamma to inhibit the intracellular replication of T. cruzi in mouse peritoneal macrophages. Thus, in addition to its known ability to inhibit the production of IFN-gamma, IL-10 (cytokine synthesis inhibitory factor), may also inhibit the effects of IFN-gamma. These experiments demonstrate that IL-10 is produced during infection with a protozoan parasite and suggest a regulatory role for this cytokine in the mediation of susceptibility to acute disease.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Bystrom, J., Evans, I., Newson, J., Stables, M., Toor, I., van Rooijen, N., Crawford, M., Colville-Nash, P., Farrow, S., Gilroy, D. W. (2008). Resolution-phase macrophages possess a unique inflammatory phenotype that is controlled by cAMP. Blood 112: 4117-4127 [Abstract] [Full Text]
Koga, R., Hamano, S., Kuwata, H., Atarashi, K., Ogawa, M., Hisaeda, H., Yamamoto, M., Akira, S., Himeno, K., Matsumoto, M., Takeda, K. (2006). TLR-Dependent Induction of IFN-beta Mediates Host Defense against Trypanosoma cruzi. J. Immunol. 177: 7059-7066 [Abstract] [Full Text]
Fraser, D. A., Bohlson, S. S., Jasinskiene, N., Rawal, N., Palmarini, G., Ruiz, S., Rochford, R., Tenner, A. J. (2006). C1q and MBL, components of the innate immune system, influence monocyte cytokine expression. J. Leukoc. Biol. 80: 107-116 [Abstract] [Full Text]
Hardison, J. L., Wrightsman, R. A., Carpenter, P. M., Lane, T. E., Manning, J. E. (2006). The Chemokines CXCL9 and CXCL10 Promote a Protective Immune Response but Do Not Contribute to Cardiac Inflammation following Infection with Trypanosoma cruzi. Infect. Immun. 74: 125-134 [Abstract] [Full Text]
Duthie, M. S., Kahn, M., White, M., Kapur, R. P., Kahn, S. J. (2005). Critical Proinflammatory and Anti-Inflammatory Functions of Different Subsets of CD1d-Restricted Natural Killer T Cells during Trypanosoma cruzi Infection. Infect. Immun. 73: 181-192 [Abstract] [Full Text]
Martins, G. A., Tadokoro, C. E., Silva, R. B., Silva, J. S., Rizzo, L. V. (2004). CTLA-4 Blockage Increases Resistance to Infection with the Intracellular Protozoan Trypanosoma cruzi. J. Immunol. 172: 4893-4901 [Abstract] [Full Text]
Arantes, R. M.E., Marche, H. H.F., Bahia, M. T., Cunha, F. Q., Rossi, M. A., Silva, J. S. (2004). Interferon-{gamma}-Induced Nitric Oxide Causes Intrinsic Intestinal Denervation in Trypanosoma cruzi-Infected Mice. Am. J. Pathol. 164: 1361-1368 [Abstract] [Full Text]
Lu, F., Huang, S., Kasper, L. H. (2003). Interleukin-10 and Pathogenesis of Murine Ocular Toxoplasmosis. Infect. Immun. 71: 7159-7163 [Abstract] [Full Text]
Boscardin, S. B., Kinoshita, S. S., Fujimura, A. E., Rodrigues, M. M. (2003). Immunization with cDNA Expressed by Amastigotes of Trypanosoma cruzi Elicits Protective Immune Response against Experimental Infection. Infect. Immun. 71: 2744-2757 [Abstract] [Full Text]
Gomes, J. A. S., Bahia-Oliveira, L. M. G., Rocha, M. O. C., Martins-Filho, O. A., Gazzinelli, G., Correa-Oliveira, R. (2003). Evidence that Development of Severe Cardiomyopathy in Human Chagas' Disease Is Due to a Th1-Specific Immune Response. Infect. Immun. 71: 1185-1193 [Abstract] [Full Text]
Olivieri, B. P., Cotta-de-Almeida, V., Araujo-Jorge, T. (2002). Benznidazole Treatment following Acute Trypanosoma cruzi Infection Triggers CD8+ T-Cell Expansion and Promotes Resistance to Reinfection. Antimicrob. Agents Chemother. 46: 3790-3796 [Abstract] [Full Text]
Goni, O., Alcaide, P., Fresno, M. (2002). Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1+)CD11b+ immature myeloid suppressor cells. Int Immunol 14: 1125-1134 [Abstract] [Full Text]
Talvani, A., Machado, F. S., Santana, G. C., Klein, A., Barcelos, L., Silva, J. S., Teixeira, M. M. (2002). Leukotriene B4 Induces Nitric Oxide Synthesis in Trypanosoma cruzi-Infected Murine Macrophages and Mediates Resistance to Infection. Infect. Immun. 70: 4247-4253 [Abstract] [Full Text]
Giambartolomei, G. H., Dennis, V. A., Lasater, B. L., Murthy, P. K., Philipp, M. T. (2002). Autocrine and Exocrine Regulation of Interleukin-10 Production in THP-1 Cells Stimulated with Borrelia burgdorferi Lipoproteins. Infect. Immun. 70: 1881-1888 [Abstract] [Full Text]
Muller, U., Kohler, G., Mossmann, H., Schaub, G. A., Alber, G., Di Santo, J. P., Brombacher, F., Holscher, C. (2001). IL-12-Independent IFN-{gamma} Production by T Cells in Experimental Chagas' Disease Is Mediated by IL-18. J. Immunol. 167: 3346-3353 [Abstract] [Full Text]
Nishikawa, Y., Tragoolpua, K., Inoue, N., Makala, L., Nagasawa, H., Otsuka, H., Mikami, T. (2001). In the Absence of Endogenous Gamma Interferon, Mice Acutely Infected with Neospora caninum Succumb to a Lethal Immune Response Characterized by Inactivation of Peritoneal Macrophages. CVI 8: 811-817 [Abstract] [Full Text]
Saeftel, M., Fleischer, B., Hoerauf, A. (2001). Stage-Dependent Role of Nitric Oxide in Control of Trypanosoma cruzi Infection. Infect. Immun. 69: 2252-2259 [Abstract] [Full Text]
Kumar, S., Tarleton, R. L. (2001). Antigen-Specific Th1 But Not Th2 Cells Provide Protection from Lethal Trypanosoma cruzi Infection in Mice. J. Immunol. 166: 4596-4603 [Abstract] [Full Text]
Aliberti, J. C. S., Souto, J. T., Marino, A. P. M. P., Lannes-Vieira, J., Teixeira, M. M., Farber, J., Gazzinelli, R. T., Silva, J. S. (2001). Modulation of Chemokine Production and Inflammatory Responses in Interferon-{{gamma}}- and Tumor Necrosis Factor-R1-Deficient Mice during Trypanosoma cruzi Infection. Am. J. Pathol. 158: 1433-1440 [Abstract] [Full Text]
Abrahamsohn, I. A., da Silva, A. P. G., Coffman, R. L. (2000). Effects of Interleukin-4 Deprivation and Treatment on Resistance to Trypanosoma cruzi. Infect. Immun. 68: 1975-1979 [Abstract] [Full Text]
Brightbill, H. D., Plevy, S. E., Modlin, R. L., Smale, S. T. (2000). A Prominent Role for Sp1 During Lipopolysaccharide- Mediated Induction of the IL-10 Promoter in Macrophages. J. Immunol. 164: 1940-1951 [Abstract] [Full Text]
Suffia, I., Ferrua, B., Stien, X., Mograbi, B., Marty, P., Rousseau, D., Fragaki, K., Kubar, J. (2000). A Novel Leishmania infantum Recombinant Antigen Which Elicits Interleukin 10 Production by Peripheral Blood Mononuclear Cells of Patients with Visceral Leishmaniasis. Infect. Immun. 68: 630-636 [Abstract] [Full Text]
Aliberti, J. C. S., Machado, F. S., Souto, J. T., Campanelli, A. P., Teixeira, M. M., Gazzinelli, R. T., Silva, J. S. (1999). beta -Chemokines Enhance Parasite Uptake and Promote Nitric Oxide-Dependent Microbiostatic Activity in Murine Inflammatory Macrophages Infected with Trypanosoma cruzi. Infect. Immun. 67: 4819-4826 [Abstract] [Full Text]
Tinkle, S. S., Kittle, L. A., Newman, L. S. (1999). Partial IL-10 Inhibition of the Cell-Mediated Immune Response in Chronic Beryllium Disease. J. Immunol. 163: 2747-2753 [Abstract] [Full Text]
Rodrigues, M. M., Ribeirao, M., Pereira-Chioccola, V., Renia, L., Costa, F. (1999). Predominance of CD4 Th1 and CD8 Tc1 Cells Revealed by Characterization of the Cellular Immune Response Generated by Immunization with a DNA Vaccine Containing a Trypanosoma cruzi Gene. Infect. Immun. 67: 3855-3863 [Abstract] [Full Text]
Aliberti, J. C.�S., Machado, F. S., Gazzinelli, R. T., Teixeira, M. M., Silva, J. S. (1999). Platelet-Activating Factor Induces Nitric Oxide Synthesis in Trypanosoma cruzi-Infected Macrophages and Mediates Resistance to Parasite Infection in Mice. Infect. Immun. 67: 2810-2814 [Abstract] [Full Text]
Millar, A. E., Wleklinski-Lee, M., Kahn, S. J. (1999). The Surface Protein Superfamily of Trypanosoma cruzi Stimulates a Polarized Th1 Response That Becomes Anergic. J. Immunol. 162: 6092-6099 [Abstract] [Full Text]
Osborne, J., Devaney, E. (1999). Interleukin-10 and Antigen-Presenting Cells Actively Suppress Th1 Cells in BALB/c Mice Infected with the Filarial Parasite Brugia pahangi. Infect. Immun. 67: 1599-1605 [Abstract] [Full Text]
Chaussabel, D., Jacobs, F., de Jonge, J., de Veerman, M., Carlier, Y., Thielemans, K., Goldman, M., Vray, B. (1999). CD40 Ligation Prevents Trypanosoma cruzi Infection through Interleukin-12 Upregulation. Infect. Immun. 67: 1929-1934 [Abstract] [Full Text]
Michailowsky, V., Murta, S. M.�F., Carvalho-Oliveira, L., Pereira, M. E.�S., Ferreira, L. R.�P., Brener, Z., Romanha, A. J., Gazzinelli, R. T. (1998). Interleukin-12 Enhances In Vivo Parasiticidal Effect of Benznidazole during Acute Experimental Infection with a Naturally Drug-Resistant Strain of Trypanosoma cruzi. Antimicrob. Agents Chemother. 42: 2549-2556 [Abstract] [Full Text]
Nasarre, C., Krahenbuhl, J. L., Klei, T. R. (1998). Down Regulation of Macrophage Activation in Brugia pahangi-Infected Jirds (Meriones unguiculatus). Infect. Immun. 66: 1063-1069 [Abstract] [Full Text]
Hua, S.-B., Wang, C. C. (1997). Interferon-gamma Activation of a Mitogen-activated Protein Kinase, KFR1, in the Bloodstream Form of Trypanosoma brucei. J. Biol. Chem. 272: 10797-10803 [Abstract] [Full Text]
Hsieh, C., Macatonia, S., Tripp, C., Wolf, S., O'Garra, A, Murphy, K. (1993). Development of TH1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages. Science 260: 547-549 [Abstract]
Barral-Netto, M, Barral, A, Brownell, C., Skeiky, Y., Ellingsworth, L., Twardzik, D., Reed, S. (1992). Transforming growth factor-beta in leishmanial infection: a parasite escape mechanism. Science 257: 545-548 [Abstract]