RMA/S cells present endogenously synthesized cytosolic proteins to class I-restricted cytotoxic T lymphocytes

doi:10.1084/jem.175.1.163

This Article

	Full Text (PDF, 532K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Esquivel, F.
	Articles by Bennink, J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Esquivel, F.
	Articles by Bennink, J.


Social Bookmarking

	What's this?

ARTICLES

RMA/S cells present endogenously synthesized cytosolic proteins to class I-restricted cytotoxic T lymphocytes

F Esquivel, J Yewdell and J Bennink
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

RMA/S is a mutant cell line with decreased cell surface expression of major histocompatibility complex class I molecules that has been reported to be deficient in presenting endogenously synthesized influenza virus nucleoprotein (NP) to cytotoxic T lymphocytes (CTL). In the present study we show that RMA/S cells can present vesicular stomatitis virus nucleocapsid protein, and, under some conditions, NP, to Kb-and Db-restricted CTL, respectively. Antigen presentation results from processing of cytosolic pools of endogenously synthesized proteins, and not the binding to cell surface class I molecules of antigenic peptides present in the virus inoculum or released from infected cells. Antigen processing of RMA/S differs, however, from processing by wild-type cells in requiring greater amounts of antigen, longer times to assemble or transport class I-peptide complexes, and in being more sensitive to blocking by anti-CD8 antibody. Thus, the antigen processing deficit in RMA/S cells is of a partial rather than absolute nature.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Goldwich, A., Hahn, S. S. C., Schreiber, S., Meier, S., Kampgen, E., Wagner, R., Lutz, M. B., Schubert, U. (2008). Targeting HIV-1 Gag into the Defective Ribosomal Product Pathway Enhances MHC Class I Antigen Presentation and CD8+ T Cell Activation. J. Immunol. 180: 372-382 [Abstract] [Full Text]
Milner, E., Barnea, E., Beer, I., Admon, A. (2006). The Turnover Kinetics of Major Histocompatibility Complex Peptides of Human Cancer Cells. Mol. Cell. Proteomics 5: 357-365 [Abstract] [Full Text]
Bulik, S., Peters, B., Holzhutter, H.-G. (2005). Quantifying the Contribution of Defective Ribosomal Products to Antigen Production: A Model-Based Computational Analysis. J. Immunol. 175: 7957-7964 [Abstract] [Full Text]
Kudo-Saito, C., Schlom, J., Camphausen, K., Coleman, C. N., Hodge, J. W. (2005). The Requirement of Multimodal Therapy (Vaccine, Local Tumor Radiation, and Reduction of Suppressor Cells) to Eliminate Established Tumors. Clin. Cancer Res. 11: 4533-4544 [Abstract] [Full Text]
Kudo-Saito, C., Schlom, J., Hodge, J. W. (2005). Induction of an Antigen Cascade by Diversified Subcutaneous/Intratumoral Vaccination Is Associated with Antitumor Responses. Clin. Cancer Res. 11: 2416-2426 [Abstract] [Full Text]
Chakraborty, M., Abrams, S. I., Coleman, C. N., Camphausen, K., Schlom, J., Hodge, J. W. (2004). External Beam Radiation of Tumors Alters Phenotype of Tumor Cells to Render Them Susceptible to Vaccine-Mediated T-Cell Killing. Cancer Res. 64: 4328-4337 [Abstract] [Full Text]
Hodge, J. W., Poole, D. J., Aarts, W. M., Gomez Yafal, A., Gritz, L., Schlom, J. (2003). Modified Vaccinia Virus Ankara Recombinants Are as Potent as Vaccinia Recombinants in Diversified Prime and Boost Vaccine Regimens to Elicit Therapeutic Antitumor Responses. Cancer Res. 63: 7942-7949 [Abstract] [Full Text]
Chakraborty, M., Abrams, S. I., Camphausen, K., Liu, K., Scott, T., Coleman, C. N., Hodge, J. W. (2003). Irradiation of Tumor Cells Up-Regulates Fas and Enhances CTL Lytic Activity and CTL Adoptive Immunotherapy. J. Immunol. 170: 6338-6347 [Abstract] [Full Text]
Hodge, J. W., Grosenbach, D. W., Aarts, W. M., Poole, D. J., Schlom, J. (2003). Vaccine Therapy of Established Tumors in the Absence of Autoimmunity. Clin. Cancer Res. 9: 1837-1849 [Abstract] [Full Text]
Cho, Y., Basta, S., Chen, W., Bennink, J. R., Yewdell, J. W. (2003). Heat-Aggregated Noninfectious Influenza Virus Induces a More Balanced CD8+-T-Lymphocyte Immunodominance Hierarchy Than Infectious Virus. J. Virol. 77: 4679-4684 [Abstract] [Full Text]
Aarts, W. M., Schlom, J., Hodge, J. W. (2002). Vector-based Vaccine/Cytokine Combination Therapy to Enhance Induction of Immune Responses to a Self-Antigen and Antitumor Activity. Cancer Res. 62: 5770-5777 [Abstract] [Full Text]
Momburg, F., Müllbacher, A., Lobigs, M. (2001). Modulation of Transporter Associated with Antigen Processing (TAP)-Mediated Peptide Import into the Endoplasmic Reticulum by Flavivirus Infection. J. Virol. 75: 5663-5671 [Abstract] [Full Text]
Grosenbach, D. W., Barrientos, J. C., Schlom, J., Hodge, J. W. (2001). Synergy of Vaccine Strategies to Amplify Antigen-specific Immune Responses and Antitumor Effects. Cancer Res. 61: 4497-4505 [Abstract] [Full Text]
Yewdell, J., Schubert, U, Bennink, J. (2001). At the crossroads of cell biology and immunology: DRiPs and other sources of peptide ligands for MHC class I molecules. J. Cell Sci. 114: 845-851 [Abstract]
Tsujimura, K., Obata, Y., Iwase, S., Matsudaira, Y., Ozeki, S., Takahashi, T. (2000). The epitope detected by cytotoxic T lymphocytes against thymus leukemia (TL) antigen is TAP independent. Int Immunol 12: 1217-1225 [Abstract] [Full Text]
Hodge, J. W., Rad, A. N., Grosenbach, D. W., Sabzevari, H., Yafal, A. G., Gritz, L., Schlom, J. (2000). Enhanced Activation of T Cells by Dendritic Cells Engineered to Hyperexpress a Triad of Costimulatory Molecules. JNCI J Natl Cancer Inst 92: 1228-1239 [Abstract] [Full Text]
De Silva, A. D., Boesteanu, A., Song, R., Nagy, N., Harhaj, E., Harding, C. V., Joyce, S. (1999). Thermolabile H-2Kb Molecules Expressed by Transporter Associated with Antigen Processing-Deficient RMA-S Cells Are Occupied by Low-Affinity Peptides. J. Immunol. 163: 4413-4420 [Abstract] [Full Text]
Chang, C. S., Brossay, L., Kronenberg, M., Kane, K. P. (1999). The Murine Nonclassical Class I Major Histocompatibility Complex-like CD1.1 Molecule Protects Target Cells from Lymphokine-activated Killer Cell Cytolysis. JEM 189: 483-491 [Abstract] [Full Text]
Dabhi, V. M., Hovik, R., Van Kaer, L., Fischer Lindahl, K. (1998). The Alloreactive T Cell Response Against the Class Ib Molecule H2-M3 Is Specific for High Affinity Peptides. J. Immunol. 161: 5171-5178 [Abstract] [Full Text]
Shi, Y., Smith, K. D., Lutz, C. T. (1998). TAP-Independent MHC Class I Peptide Antigen Presentation to Alloreactive CTL Is Enhanced by Target Cell Incubation at Subphysiologic Temperatures. J. Immunol. 160: 4305-4312 [Abstract] [Full Text]
Wolpert, E. Z., Petersson, M., Chambers, B. J., Sandberg, J. K., Kiessling, R., Ljunggren, H.-G., Karre, K. (1997). Generation of CD8+ T cells specific for transporter associated with antigen processing deficient�cells. Proc. Natl. Acad. Sci. USA 94: 11496-11501 [Abstract] [Full Text]
Snyder, H. L., Bacik, I., Bennink, J. R., Kearns, G., Behrens, T. W., Bachi, T., Orlowski, M., Yewdell, J. W. (1997). Two Novel Routes of Transporter Associated with Antigen Processing (TAP)-independent Major Histocompatibility Complex Class I Antigen Processing. JEM 186: 1087-1098 [Abstract] [Full Text]
Bacik, I., Snyder, H. L., Anton, L. C., Russ, G., Chen, W., Bennink, J. R., Urge, L., Otvos, L., Dudkowska, B., Eisenlohr, L., Yewdell, J. W. (1997). Introduction of a Glycosylation Site into a Secreted Protein Provides Evidence for an Alternative Antigen Processing Pathway: Transport of Precursors of Major Histocompatability Complex Class I-Restricted Peptides from the Endoplasmic Reticulum to the Cytosol. JEM 186: 479-487 [Abstract] [Full Text]
Smith, P. A., Brunmark, A., Jackson, M. R., Potter, T. A. (1997). Peptide-independent Recognition by Alloreactive Cytotoxic T Lymphocytes (CTL). JEM 185: 1023-1034 [Abstract] [Full Text]