Presentation of antigen by mixed isotype class II molecules in normal H- 2d mice

doi:10.1084/jem.175.1.157

This Article

	Full Text (PDF, 659K)
	Alert me when this article is cited

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ruberti, G.
	Articles by Livingstone, A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ruberti, G.
	Articles by Livingstone, A.


Social Bookmarking

	What's this?

ARTICLES

Presentation of antigen by mixed isotype class II molecules in normal H- 2d mice

G Ruberti, KS Sellins, CM Hill, RN Germain, CG Fathman and A Livingstone
Department of Medicine, Stanford University School of Medicine, California 94305.

A panel of DBA/2 T cell hybridomas specific for the sperm whale myoglobin epitope 110-121 was found to recognize antigen presented by the mixed isotype class II molecule E alpha dA beta d. The response was blocked by monoclonal antibodies specific for E alpha and A beta d chains; in addition, the hybridomas responded to antigen presented by L cells expressing E alpha A beta d molecules, and made no response with L cells expressing I-Ad or I-Ed molecules. Two more groups of hybridomas isolated from DBA/2 and B10.D2 mice immunized with myoglobin also recognized peptide 110-121 presented by E alpha d A beta d. Thus, although it is expressed at biochemically undetectable levels on spleen cells, the E alpha d A beta d molecule is an important presenting element in normal H-2d mice making a conventional immune response to a protein antigen. These results suggest that high levels of class II expression are not a prerequisite for T cell activation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Martinez-Soria, E., Santiago-Raber, M.-L., Ho, L., Moll, T., Izui, S. (2008). Protection of Murine Systemic Lupus by the Ea Transgene without Expression of I-E Heterodimers. J. Immunol. 181: 3651-3657 [Abstract] [Full Text]
Williams, K. M., Bigley, E. C. III (2004). Identification of an I-Ed-Restricted T-Cell Epitope of Escherichia coli Outer Membrane Protein F. Infect. Immun. 72: 3907-3913 [Abstract] [Full Text]
Fasso, M., Anandasabapathy, N., Crawford, F., Kappler, J., Fathman, C. G., Ridgway, W. M. (2000). T Cell Receptor (TCR)-mediated Repertoire Selection and Loss of TCR V{beta} Diversity during the Initiation of a CD4+ T Cell Response In Vivo. JEM 192: 1719-1730 [Abstract] [Full Text]
JABRANE-FERRAT, N., BLOOM, D., WU, A., LI, L., LO, D., SREEDHARAN, S. P., TURCK, C. W., GOETZL, A. E. J. (1999). Enhancement by vasoactive intestinal peptide of {gamma}-interferon production by antigen-stimulated type 1 helper T cells. FASEB J. 13: 347-353 [Abstract] [Full Text]