CD69-mediated pathway of lymphocyte activation: anti-CD69 monoclonal antibodies trigger the cytolytic activity of different lymphoid effector cells with the exception of cytolytic T lymphocytes expressing T cell receptor alpha/beta

doi:10.1084/jem.174.6.1393

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CD69-mediated pathway of lymphocyte activation: anti-CD69 monoclonal antibodies trigger the cytolytic activity of different lymphoid effector cells with the exception of cytolytic T lymphocytes expressing T cell receptor alpha/beta

A Moretta, A Poggi, D Pende, G Tripodi, AM Orengo, N Pella, R Augugliaro, C Bottino, E Ciccone and L Moretta
Istituto di Istologia ed Embriologia Generale, Universita di Genova, Italy.

The effect of anti-CD69 monoclonal antibodies (mAbs) on the induction of the cytolytic activity in different types of lymphoid effector cells has been investigated. Three anti-CD69 mAbs, including the reference mAb MLR3 and two new mAbs (c227 and 31C4), have been used. All cloned CD3-CD16+ natural killer (NK) cells belonging to different subsets (as defined by the surface expression of GL183 and/or EB6 antigens) were efficiently triggered by anti-CD69 mAbs and lysed P815 mastocytoma cells in a redirected killing assay. Triggering of the cytolytic activity could also be induced in CD3-CD16- NK clones, which fail to respond to other stimuli (including anti-CD16, anti-CD2 mAbs, or phytohemagglutinin). A similar triggering effect was detected in T cell receptor (TCR) gamma/delta+ clones belonging to different subsets. On the other hand, anti-CD69 mAbs could not induce triggering of the cytolytic activity in TCR alpha/beta+ cytolytic clones. Since all thymocytes are known to express CD69 antigen after cell activation, we analyzed a series of phenotypically different cytolytic thymocyte populations and clones for their responsiveness to anti-CD69 mAb in a redirected killing assay. Again, anti-CD69 mAb triggered TCR gamma/delta+ but not TCR alpha/beta+ thymocytes. Anti-CD69 mAb efficiently triggered the cytolytic activity of "early" thymocytes lines or clones (CD3-4-8-7+), which lack all other known pathways of cell activation. Thus, it appears that CD69 molecules may initiate a pathway of activation of cytolytic functions common to a number of activated effector lymphocytes with the remarkable exception of TCR alpha/beta+ cytolytic cells.
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Bernard, K., Cambiaggi, A., Guia, S., Bertucci, F., Granjeaud, S., Tagett, R., N'Guyen, C., Jordan, B. R., Vivier, E. (1999). Engagement of Natural Cytotoxicity Programs Regulates AP-1 Expression in the NKL Human NK Cell Line. J. Immunol. 162: 4062-4068 [Abstract] [Full Text]
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Lahn, M., Kalataradi, H., Mittelstadt, P., Pflum, E., Vollmer, M., Cady, C., Mukasa, A., Vella, A. T., Ikle, D., Harbeck, R., O'Brien, R., Born, W. (1998). Early Preferential Stimulation of {gamma}{delta} T Cells by TNF-{alpha}. J. Immunol. 160: 5221-5230 [Abstract] [Full Text]
Brumbaugh, K. M., Binstadt, B. A., Billadeau, D. D., Schoon, R. A., Dick, C. J., Ten, R. M., Leibson, P. J. (1997). Functional Role for Syk Tyrosine Kinase in Natural Killer Cell-mediated Natural Cytotoxicity. J. Exp. Med. 186: 1965-1974 [Abstract] [Full Text]
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Vance, B. A., Wu, W., Ribaudo, R. K., Segal, D. M., Kearse, K. P. (1997). Multiple Dimeric Forms of Human CD69 Result from Differential Addition of N-Glycans to Typical (Asn-X-Ser/Thr) and Atypical (Asn-X-Cys) Glycosylation Motifs. J. Biol. Chem. 272: 23117-23122 [Abstract] [Full Text]
Sconocchia, G., Titus, J. A., Segal, D. M. (1997). Signaling Pathways Regulating CD44-Dependent Cytolysis in Natural Killer Cells. Blood 90: 716-725 [Abstract] [Full Text]
Carbone, E., Ruggiero, G., Terrazzano, G., Palomba, C., Manzo, C., Fontana, S., Spits, H., Karre, K., Zappacosta, S. (1997). A New Mechanism of NK Cell Cytotoxicity Activation: The CD40-CD40 Ligand Interaction. J. Exp. Med. 185: 2053-2060 [Abstract] [Full Text]
Hardy, B., Kovjazin, R., Raiter, A., Ganor, N., Novogrodsky, A. (1997). A lymphocyte-activating monoclonal antibody induces regression of human tumors in severe combined immunodeficient�mice. Proc. Natl. Acad. Sci. USA 94: 5756-5760 [Abstract] [Full Text]
L�pez-Cabrera, M., Mu�oz, E., Bl�zquez, M. V., Ursa, M. A., Santis, A. G., S�nchez-Madrid, F. (1995). Transcriptional Regulation of the Gene Encoding the Human C-type Lectin Leukocyte Receptor AIM/CD69 and Functional Characterization of Its Tumor Necrosis Factor-alpha-responsive Elements. J. Biol. Chem. 270: 21545-21551 [Abstract] [Full Text]