Cloning of murine TCF-1, a T cell-specific transcription factor interacting with functional motifs in the CD3-epsilon and T cell receptor alpha enhancers

doi:10.1084/jem.173.5.1133

This Article

	Full Text (PDF, 1468K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Oosterwegel, M.
	Articles by Clevers, H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Oosterwegel, M.
	Articles by Clevers, H.


Social Bookmarking

	What's this?

ARTICLES

Cloning of murine TCF-1, a T cell-specific transcription factor interacting with functional motifs in the CD3-epsilon and T cell receptor alpha enhancers

M Oosterwegel, M van de Wetering, D Dooijes, L Klomp, A Winoto, K Georgopoulos, F Meijlink and H Clevers
Department of Clinical Immunology, University Hospital Utrecht, The Netherlands.

CD3-epsilon gene expression is confined to the T cell lineage. We have recently identified and cloned a human transcription factor, TCF-1, that binds to a functional element in the T lymphocyte-specific enhancer of CD3-epsilon. In a panel of human cell lines, TCF-1 expression was restricted to T lineage cells. TCF-1 belonged to a novel family of genes that contain the so-called high mobility group 1 (HMG) box. Here we report the cloning of murine TCF-1. Two splice alternatives were identified that were not previously observed in human TCF-1. Murine and human TCF-1 displayed a 95.5% overall amino acid homology. Recombinant murine and human TCF-1 recognized the same sequence motif in the CD3-epsilon enhancer as judged by gel retardation and methylation interference assays. With the murine cDNA clones several aspects of TCF-1 were analyzed. First, deletion analysis revealed that a region of TCF-1 containing the HMG box was sufficient for sequence-specific binding. Second, by high stringency Northern blotting and in situ hybridization, TCF-1 expression was shown to be confined to the thymus and to the T cell areas of the spleen. Third, TCF-1 bound specifically to a functional T cell-specific element in the T cell receptor alpha (TCR-alpha) enhancer. The T lineage-specific expression and the affinity for functional motifs in the TCR-alpha and CD3-epsilon enhancers imply an important role for TCF-1 in the establishment of the mature T cell phenotype.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Zhang, C., Cho, K., Huang, Y., Lyons, J. P., Zhou, X., Sinha, K., McCrea, P. D., de Crombrugghe, B. (2008). Inhibition of Wnt signaling by the osteoblast-specific transcription factor Osterix. Proc. Natl. Acad. Sci. USA 105: 6936-6941 [Abstract] [Full Text]
Choi, K. H., Park, M. W., Lee, S. Y., Jeon, M.-Y., Kim, M. Y., Lee, H. K., Yu, J., Kim, H.-J., Han, K., Lee, H., Park, K., Park, W. J., Jeong, S. (2006). Intracellular expression of the T-cell factor-1 RNA aptamer as an intramer.. Molecular Cancer Therapeutics 5: 2428-2434 [Abstract] [Full Text]
Ioannidis, V., Kunz, B., Tanamachi, D. M., Scarpellino, L., Held, W. (2003). Initiation and Limitation of Ly-49A NK Cell Receptor Acquisition by T Cell Factor-1. J. Immunol. 171: 769-775 [Abstract] [Full Text]
Dorfman, D. M., Greisman, H. A., Shahsafaei, A. (2003). Loss of Expression of the WNT/{beta}-Catenin-Signaling Pathway Transcription Factors Lymphoid Enhancer Factor-1 (LEF-1) and T Cell Factor-1 (TCF-1) in a Subset of Peripheral T Cell Lymphomas. Am. J. Pathol. 162: 1539-1544 [Abstract] [Full Text]
Taghon, T., Stolz, F., De Smedt, M., Cnockaert, M., Verhasselt, B., Plum, J., Leclercq, G. (2002). HOX-A10 regulates hematopoietic lineage commitment: evidence for a monocyte-specific transcription factor. Blood 99: 1197-1204 [Abstract] [Full Text]
El-Tanani, M., Barraclough, R., Wilkinson, M. C., Rudland, P. S. (2001). Metastasis-inducing DNA Regulates the Expression of the Osteopontin Gene by Binding the Transcription Factor Tcf-4. Cancer Res. 61: 5619-5629 [Abstract] [Full Text]
Staal, F. J.T., Weerkamp, F., Langerak, A. W., Hendriks, R. W., Clevers, H. C. (2001). Transcriptional Control of T Lymphocyte Differentiation. Stem Cells 19: 165-179 [Abstract] [Full Text]
Diehl, N. L., Enslen, H., Fortner, K. A., Merritt, C., Stetson, N., Charland, C., Flavell, R. A., Davis, R. J., Rincon, M. (2000). Activation of the p38 Mitogen-activated Protein Kinase Pathway Arrests Cell Cycle Progression and Differentiation of Immature Thymocytes In Vivo. JEM 191: 321-334 [Abstract] [Full Text]
Ohmura, K., Kawamoto, H., Fujimoto, S., Ozaki, S., Nakao, K., Katsura, Y. (1999). Emergence of T, B, and Myeloid Lineage-Committed as well as Multipotent Hemopoietic Progenitors in the Aorta-Gonad- Mesonephros Region of Day 10 Fetuses of the Mouse. J. Immunol. 163: 4788-4795 [Abstract] [Full Text]
Barndt, R., Dai, M.-F., Zhuang, Y. (1999). A Novel Role for HEB Downstream or Parallel to the Pre-TCR Signaling Pathway During {alpha}{beta} Thymopoiesis. J. Immunol. 163: 3331-3343 [Abstract] [Full Text]
Kawamoto, H., Ohmura, K., Fujimoto, S., Katsura, Y. (1999). Emergence of T Cell Progenitors Without B Cell or Myeloid Differentiation Potential at the Earliest Stage of Hematopoiesis in the Murine Fetal Liver. J. Immunol. 162: 2725-2731 [Abstract] [Full Text]
Tokoro, Y., Sugawara, T., Yaginuma, H., Nakauchi, H., Terhorst, C., Wang, B., Takahama, Y. (1998). A Mouse Carrying Genetic Defect in the Choice Between T and B Lymphocytes. J. Immunol. 161: 4591-4598 [Abstract] [Full Text]
Schilham, M. W., Wilson, A., Moerer, P., Benaissa-Trouw, B. J., Cumano, A., Clevers, H. C. (1998). Critical Involvement of Tcf-1 in Expansion of Thymocytes. J. Immunol. 161: 3984-3991 [Abstract] [Full Text]
Korinek, V., Barker, N., Willert, K., Molenaar, M., Roose, J., Wagenaar, G., Markman, M., Lamers, W., Destree, O., Clevers, H. (1998). Two Members of the Tcf Family Implicated in Wnt/beta -Catenin Signaling during Embryogenesis in the Mouse. Mol. Cell. Biol. 18: 1248-1256 [Abstract] [Full Text]
Orkin, S. H., Orkin, S. H. (1995). Transcription Factors and Hematopoietic Development. J. Biol. Chem. 270: 4955-4958 [Full Text]
van Genderen, C, Okamura, R M, Farinas, I, Quo, R G, Parslow, T G, Bruhn, L, Grosschedl, R (1994). Development of several organs that require inductive epithelial-mesenchymal interactions is impaired in LEF-1-deficient mice.. Genes Dev. 8: 2691-2703 [Abstract]
Oosterwegel, M, van de Wetering, M, Timmerman, J, Kruisbeek, A, Destree, O, Meijlink, F, Clevers, H (1993). Differential expression of the HMG box factors TCF-1 and LEF-1 during murine embryogenesis. Development 118: 439-448 [Abstract]
Harley, V., Jackson, D., Hextall, P., Hawkins, JR, Berkovitz, G., Sockanathan, S, Lovell-Badge, R, Goodfellow, P. (1992). DNA binding activity of recombinant SRY from normal males and XY females. Science 255: 453-456 [Abstract]
Giese, K, Amsterdam, A, Grosschedl, R (1991). DNA-binding properties of the HMG domain of the lymphoid-specific transcriptional regulator LEF-1.. Genes Dev. 5: 2567-2578 [Abstract]