Molecular characterization of an autoantigen of PM-Scl in the polymyositis/scleroderma overlap syndrome: a unique and complete human cDNA encoding an apparent 75-kD acidic protein of the nucleolar complex

doi:10.1084/jem.173.4.941

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Alderuccio, F.
	Articles by Tan, E. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Alderuccio, F.
	Articles by Tan, E. M.


Social Bookmarking

	What's this?

ARTICLES

Molecular characterization of an autoantigen of PM-Scl in the polymyositis/scleroderma overlap syndrome: a unique and complete human cDNA encoding an apparent 75-kD acidic protein of the nucleolar complex

F Alderuccio, EK Chan and EM Tan
Department of Molecular and Experimental Medicine, Scripps Clinic and Research Foundation, La Jolla, California 92037.

About 50% of patients with the polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a nuclear/nucleolar particle termed PM-Scl. The particle is composed of several polypeptides of which two have been identified as autoantigens. In this report, human cDNA clone coding for the entire 75-kD autoantigen of the PM-Scl particle (PM-Scl 75) was isolated from a MOLT- 4 lambda gt-11 library. The deduced amino acid sequence of the cDNA clone represented a protein of 355 amino acids and 39.2 kD; the in vitro translation product of this cDNA migrated in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) at approximately 70 kD. The aberrant migration of the polypeptide in SDS-PAGE was shown to be related to the COOH half that was rich in acidic residues. Authenticity of the cDNA coding for PM-Scl 75 was shown by immunoreactivity of PM-Scl sera with in vitro translation products and recombinant fusion proteins encoded by the cDNA. In addition, rabbit antibodies raised to recombinant fusion protein reacted in immunofluorescence, immunoblotting, and immunoprecipitation with the characteristic features displayed by human anti-PM-Scl sera.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Roh, S. G., Kuno, M., Hishikawa, D., Hong, Y. H., Katoh, K., Obara, Y., Hidari, H., Sasaki, S. (2007). Identification of differentially expressed transcripts in bovine rumen and abomasum using a differential display method. J ANIM SCI 85: 395-403 [Abstract] [Full Text]
Xiong, Z, Shaibani, A, Li, Y-P, Yan, Y, Zhang, S, Yang, Y, Yang, F, Wang, H, Yang, X-F (2006). Alternative splicing factor ASF/SF2 is down regulated in inflamed muscle. J. Clin. Pathol. 59: 855-861 [Abstract] [Full Text]
Schilders, G., Raijmakers, R., Raats, J. M. H., Pruijn, G. J. M. (2005). MPP6 is an exosome-associated RNA-binding protein involved in 5.8S rRNA maturation. Nucleic Acids Res 33: 6795-6804 [Abstract] [Full Text]
Raijmakers, R., Egberts, W. V., van Venrooij, W. J., Pruijn, G. J. M. (2003). The Association of the Human PM/Scl-75 Autoantigen with the Exosome Is Dependent on a Newly Identified N Terminus. J. Biol. Chem. 278: 30698-30704 [Abstract] [Full Text]
Jury, E C, D'Cruz, D, Morrow, W J W (2001). Autoantibodies and overlap syndromes in autoimmune rheumatic disease. J. Clin. Pathol. 54: 340-347 [Abstract] [Full Text]
Brouwer, R, Hengstman, G J D, Egberts, W V., Ehrfeld, H, Bozic, B, Ghirardello, A, Grondal, G, Hietarinta, M, Isenberg, D, Kalden, J R, Lundberg, I, Moutsopoulos, H, Roux-Lombard, P, Vencovsky, J, Wikman, A, Seelig, H P, van Engelen, B G M, van Venrooij, W J (2001). Autoantibody profiles in the sera of European patients with myositis. Ann Rheum Dis 60: 116-123 [Abstract] [Full Text]
Allmang, C., Petfalski, E., Podtelejnikov, A., Mann, M., Tollervey, D., Mitchell, P. (1999). The yeast exosome and human PM-Scl are related complexes of 3' right-arrow 5' exonucleases. Genes Dev. 13: 2148-2158 [Abstract] [Full Text]
Briggs, M. W., Burkard, K. T.�D., Butler, J. S. (1998). Rrp6p, the Yeast Homologue of the Human PM-Scl 100-kDa Autoantigen, Is Essential for Efficient 5.8�S rRNA 3' End Formation. J. Biol. Chem. 273: 13255-13263 [Abstract] [Full Text]
Kho, C.-J., Huggins, G. S., Endege, W. O., Patterson, C., Jain, M. K., Lee, M.-E., Haber, E. (1997). The Polymyositis-Scleroderma Autoantigen Interacts with the Helix-Loop-Helix Proteins E12 and E47. J. Biol. Chem. 272: 13426-13431 [Abstract] [Full Text]
Girard, J.-P., Springer, T. A. (1996). Modulation of Endothelial Cell Adhesion by Hevin, an Acidic Protein Associated with High Endothelial Venules. J. Biol. Chem. 271: 4511-4517 [Abstract] [Full Text]
Minty, A., Dumont, X., Kaghad, M., Caput, D. (2000). Covalent Modification of p73alpha by SUMO-1. TWO-HYBRID SCREENING WITH p73 IDENTIFIES NOVEL SUMO-1-INTERACTING PROTEINS AND A SUMO-1 INTERACTION MOTIF. J. Biol. Chem. 275: 36316-36323 [Abstract] [Full Text]
Brouwer, R., Allmang, C., Raijmakers, R., van Aarssen, Y., Egberts, W. V., Petfalski, E., van Venrooij, W. J., Tollervey, D., Pruijn, G. J. M. (2001). Three Novel Components of the Human Exosome. J. Biol. Chem. 276: 6177-6184 [Abstract] [Full Text]
Chai, Z., Sarcevic, B., Mawson, A., Toh, B.-H. (2001). SET-related Cell Division Autoantigen-1 (CDA1) Arrests Cell Growth. J. Biol. Chem. 276: 33665-33674 [Abstract] [Full Text]