Journal of Experimental Medicine, Vol 173, 823-832, Copyright © 1991 by Rockefeller University Press
Generation of T cells with lytic specificity for atypical antigens. I. A mitochondrial antigen in the rat
JD Davies, DH Wilson, E Hermel, KF Lindahl, GW Butcher and DB Wilson
Medical Biology Institute, La Jolla, California 92037.
F1 rats primed with normal parental strain lymphocyte populations and
restimulated in culture with parental lymphoblasts generate potent
cytotoxic T cell responses to unusual antigen systems. Here we describe in
the Lewis (L)/DA anti-DA combination an antigen system most likely of
mitochondrial origin with the following properties: it is transmitted
maternally from DA strain females, inherited in an extra- chromosomal
manner, restricted by class I RT1Aa major histocompatibility complex gene
products, extinguished on target cells treated with chloramphenicol, and
its pattern of expression in different rat strains correlates with
restriction fragment-length polymorphisms of mitochondrial DNA. Sequence
analysis of the rat ND1 gene indicates that the maternally transferred
factor in the rat is not a homologue of the maternally transmitted factor
responsible for the mitochondrial antigen in mice. In keeping with its
inheritance from DA females, this antigen is present on target cells from
(DA female x L male)F1 donors and all other F1 combinations derived from DA
female parents, but absent from target cells from some F1 combinations
(L/DA and Wistar-Furth [WF]/DA) derived from DA strain males. The presence
of this antigen in other F1 combinations (Brown Norway [BN]/DA, August 2880
[AUG]/DA, and PVG/DA) indicates that this mitochondrial antigen system is
shared by the DA, BN, and PVG strains, but not by the L and WF strains.
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