Human immunodeficiency virus (HIV) infection in CD4+ T lymphocytes genetically deficient in LFA-1: LFA-1 is required for HIV-mediated cell fusion but not for viral transmission

doi:10.1084/jem.173.2.511

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Pantaleo, G.
	Articles by Fauci, A. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Pantaleo, G.
	Articles by Fauci, A. S.


Social Bookmarking

	What's this?

ARTICLES

Human immunodeficiency virus (HIV) infection in CD4+ T lymphocytes genetically deficient in LFA-1: LFA-1 is required for HIV-mediated cell fusion but not for viral transmission

G Pantaleo, L Butini, C Graziosi, G Poli, SM Schnittman, JJ Greenhouse, JI Gallin and AS Fauci
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

In the present study, we demonstrated that expression of the LFA-1 molecule is necessary for cell fusion and syncytia formation in human immunodeficiency virus (HIV)-infected CD4+ T lymphocytes. In contrast, the lack of expression of LFA-1 does not influence significantly cell- to-cell transmission of HIV. In fact, LFA-1- T lymphocytes obtained from a leukocyte adhesion deficiency patient were unable to fuse and form syncytia when infected with HIV-1 or HIV-2, despite the fact that efficiency of HIV infection (i.e., virus entry, HIV spreading, and levels of virus replication) was comparable with that observed in LFA- 1+ T lymphocytes. In addition, we provide evidence that LFA-1 by mediating cell fusion contributes to the depletion of HIV-infected CD4+ T lymphocytes in vitro.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Gilbert, C., Cantin, R., Barat, C., Tremblay, M. J. (2007). Human Immunodeficiency Virus Type 1 Replication in Dendritic Cell-T-Cell Cocultures Is Increased upon Incorporation of Host LFA-1 due to Higher Levels of Virus Production in Immature Dendritic Cells. J. Virol. 81: 7672-7682 [Abstract] [Full Text]
Kubo, Y., Ishimoto, A., Amanuma, H. (2003). N-Linked Glycosylation Is Required for XC Cell-Specific Syncytium Formation by the R Peptide-Containing Envelope Protein of Ecotropic Murine Leukemia Viruses. J. Virol. 77: 7510-7516 [Abstract] [Full Text]
Tanaka, R., Yoshida, A., Murakami, T., Baba, E., Lichtenfeld, J., Omori, T., Kimura, T., Tsurutani, N., Fujii, N., Wang, Z.-X., Peiper, S. C., Yamamoto, N., Tanaka, Y. (2001). Unique Monoclonal Antibody Recognizing the Third Extracellular Loop of CXCR4 Induces Lymphocyte Agglutination and Enhances Human Immunodeficiency Virus Type 1-Mediated Syncytium Formation and Productive Infection. J. Virol. 75: 11534-11543 [Abstract] [Full Text]
Mizuno, K., Okamoto, H., Horio, T. (2001). Muramyl dipeptide and mononuclear cell supernatant induce Langhans-type cells from human monocytes. J. Leukoc. Biol. 70: 386-394 [Abstract] [Full Text]
Blanco, J., Barretina, J., Henson, G., Bridger, G., De Clercq, E., Clotet, B., Esté, J. A. (2000). The CXCR4 Antagonist AMD3100 Efficiently Inhibits Cell-Surface-Expressed Human Immunodeficiency Virus Type 1 Envelope-Induced Apoptosis. Antimicrob. Agents Chemother. 44: 51-56 [Abstract] [Full Text]
Sommerfelt, M. A. (1999). Retrovirus receptors. J. Gen. Virol. 80: 3049-3064 [Full Text]
Sterling, H., Saginario, C., Vignery, A. (1998). CD44 Occupancy Prevents Macrophage Multinucleation. J. Cell Biol. 143: 837-847 [Abstract] [Full Text]
Barbeau, B., Fortin, J.-F., Genois, N., Tremblay, M. J. (1998). Modulation of Human Immunodeficiency Virus Type 1-Induced Syncytium Formation by the Conformational State of LFA-1 Determined by a New Luciferase-Based Syncytium Quantitative Assay. J. Virol. 72: 7125-7136 [Abstract] [Full Text]
Wyatt, R., Sodroski, J. (1998). The HIV-1 Envelope Glycoproteins: Fusogens, Antigens, and Immunogens. Science 280: 1884-1888 [Abstract] [Full Text]
Fortin, J.-F., Cantin, R., Tremblay, M. J. (1998). T Cells Expressing Activated LFA-1 Are More Susceptible to Infection with Human Immunodeficiency Virus Type 1�Particles Bearing Host-Encoded ICAM-1. J. Virol. 72: 2105-2112 [Abstract] [Full Text]
Callebaut, C., Jacotot, E., Krust, B., Guichard, G., Blanco, J., Valenzuela, A., Svab, J., Muller, S., Briand, J.-P., Hovanessian, A. G. (1997). Pseudopeptide TASP Inhibitors of HIV Entry Bind Specifically to a 95-kDa Cell Surface Protein. J. Biol. Chem. 272: 7159-7166 [Abstract] [Full Text]
Callebaut, C., Jacotot, E., Krust, B., Hovanessian, A. G. (1994). Response. Science 264: 1162-1165
Pantaleo, G., Graziosi, C., Fauci, A. S. (1993). The Immunopathogenesis of Human Immunodeficiency Virus Infection. NEJM 328: 327-335 [Full Text]
Cohen, D., Tani, Y, Tian, H, Boone, E, Samelson, L., Lane, H. (1992). Participation of tyrosine phosphorylation in the cytopathic effect of human immunodeficiency virus-1. Science 256: 542-545 [Abstract]
Puig-Kroger, A., Lopez-Rodriguez, C., Relloso, M., Sanchez-Elsner, T., Nueda, A., Munoz, E., Bernabeu, C., Corbi, A. L. (2000). Polyomavirus Enhancer-binding Protein 2/Core Binding Factor/Acute Myeloid Leukemia Factors Contribute to the Cell Type-specific Activity of the CD11a Integrin Gene Promoter. J. Biol. Chem. 275: 28507-28512 [Abstract] [Full Text]