The omega/lambda 5 surrogate immunoglobulin light chain is expressed on the surface of transitional B lymphocytes in murine bone marrow

doi:10.1084/jem.173.1.111

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Cherayil, B. J.
	Articles by Pillai, S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Cherayil, B. J.
	Articles by Pillai, S.


Social Bookmarking

	What's this?

ARTICLES

The omega/lambda 5 surrogate immunoglobulin light chain is expressed on the surface of transitional B lymphocytes in murine bone marrow

BJ Cherayil and S Pillai
Molecular Immunology Laboratory, Cancer Center of Massachusetts General Hospital, Boston.

The membrane immunoglobulin heavy chain (micron) plays a feedback role during the pre-B stage of B lymphocyte differentiation. In pre-B cell lines, micron associates with two surrogate light chain proteins. The omega chain is disulfide linked to mu and was predicted to be the product of the lambda 5 gene. The iota chain is noncovalently associated with micron. We demonstrate that the omega protein is indeed the product of the lambda 5 gene and that mu, omega, and iota are coassociated in the same complex. Antibodies against the omega/lambda 5 protein demonstrate the existence of a subpopulation of "transitional" bone marrow B cells that express micron and omega on the cell surface. The majority of these cells also express surface kappa light chains, indicating that in B lymphoid ontogeny the lambda 5 gene is inactivated after the onset of kappa light chain expression.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Bradl, H., Jack, H.-M. (2001). Surrogate Light Chain-Mediated Interaction of a Soluble Pre-B Cell Receptor with Adherent Cell Lines. J. Immunol. 167: 6403-6411 [Abstract] [Full Text]
Fang, T., Smith, B. P., Roman, C. A. J. (2001). Conventional and Surrogate Light Chains Differentially Regulate Ig {micro} and D{micro} Heavy Chain Maturation and Surface Expression. J. Immunol. 167: 3846-3857 [Abstract] [Full Text]
Donohoe, M. E., Beck-Engeser, G. B., Lonberg, N., Karasuyama, H., Riley, R. L., Jack, H.-M., Blomberg, B. B. (2000). Transgenic Human {lambda}5 Rescues the Murine {lambda}5 Nullizygous Phenotype. J. Immunol. 164: 5269-5276 [Abstract] [Full Text]
Wang, Y.-H., Nomura, J., Faye-Petersen, O. M., Cooper, M. D. (1998). Surrogate Light Chain Production During B Cell Differentiation: Differential Intracellular Versus Cell Surface Expression. J. Immunol. 161: 1132-1139 [Abstract] [Full Text]
Brouns, G. S., Vries, E. d., Neefjes, J. J., Borst, J. (1996). Assembled Pre-B Cell Receptor Complexes Are Retained in the Endoplasmic Reticulum by a Mechanism That Is Not Selective for the Pseudo-light Chain. J. Biol. Chem. 271: 19272-19278 [Abstract] [Full Text]
Spanopoulou, E, Roman, C A, Corcoran, L M, Schlissel, M S, Silver, D P, Nemazee, D, Nussenzweig, M C, Shinton, S A, Hardy, R R, Baltimore, D (1994). Functional immunoglobulin transgenes guide ordered B-cell differentiation in Rag-1-deficient mice.. Genes Dev. 8: 1030-1042 [Abstract]
Young, F, Ardman, B, Shinkai, Y, Lansford, R, Blackwell, T K, Mendelsohn, M, Rolink, A, Melchers, F, Alt, F W (1994). Influence of immunoglobulin heavy- and light-chain expression on B-cell differentiation.. Genes Dev. 8: 1043-1057 [Abstract]