T cell receptor-mediated negative selection of autoreactive T lymphocyte precursors occurs after commitment to the CD4 or CD8 lineages

doi:10.1084/jem.172.3.835

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T cell receptor-mediated negative selection of autoreactive T lymphocyte precursors occurs after commitment to the CD4 or CD8 lineages

CJ Guidos, JS Danska, CG Fathman and IL Weissman
Department of Pathology, Stanford University School of Medicine, California 94305.

To identify the maturational stage(s) during which T cell receptor (TCR)-mediated positive and negative selection occurs, we followed the development of CD4+8- and CD4-8+ T cells from TCRlo CD4+8+ thymic blasts in the presence of different positive and negative selecting (major histocompatibility complex or Mls) elements. We describe novel lineage-committed transitional intermediates that are TCRmed CD4+8lo or TCRmed CD4lo8+, and that show evidence of having been positively selected. Furthermore, negative selection is not evident until after cells have attained one of the TCRmed transitional phenotypes. Accordingly, we propose that negative selection in normal mice occurs only after TCRlo CD4+8+ precursors have been positively selected into either the CD4 or CD8 lineage.
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Williams, J. A., Sharrow, S. O., Adams, A. J., Hodes, R. J. (2002). CD40 Ligand Functions Non-Cell Autonomously to Promote Deletion of Self-Reactive Thymocytes. J. Immunol. 168: 2759-2765 [Abstract] [Full Text]
Lucas, B., Germain, R. N. (2000). Opening a Window on Thymic Positive Selection: Developmental Changes in the Influence of Cosignaling by Integrins and CD28 on Selection Events Induced by TCR Engagement. J. Immunol. 165: 1889-1895 [Abstract] [Full Text]
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Sommers, C. L., Rabin, R. L., Grinberg, A., Tsay, H. C., Farber, J., Love, P. E. (1999). A Role for the Tec Family Tyrosine Kinase Txk in T Cell Activation and Thymocyte Selection. JEM 190: 1427-1438 [Abstract] [Full Text]
Flake, A. W., Zanjani, E. D. (1999). In Utero Hematopoietic Stem Cell Transplantation: Ontogenic Opportunities and Biologic Barriers. Blood 94: 2179-2191 [Full Text]
Dyall, R., Nikolic-Zugic, J. (1999). The Final Maturation of At Least Some Single-positive CD4hi Thymocytes Does Not Require T Cell Receptor�Major Histocompatibility Complex Contact. JEM 190: 757-764 [Abstract] [Full Text]
Baldwin, K. K., Trenchak, B. P., Altman, J. D., Davis3, M. M. (1999). Negative Selection of T Cells Occurs Throughout Thymic Development. J. Immunol. 163: 689-698 [Abstract] [Full Text]
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AKASHI, K., KONDO, M., CHESHIER, S., SHIZURU, J., GANDY, K., DOMEN, J., MEBIUS, R., TRAVER, D., WEISSMAN, I.L. (1999). Lymphoid Development from Stem Cells and the Common Lymphocyte Progenitors. Cold Spring Harb Symp Quant Biol 64: 1-12 [Abstract]
Dave, V. P., Allman, D., Keefe, R., Hardy, R. R., Kappes, D. J. (1998). HD mice: A novel mouse mutant with a specific defect in the generation of CD4+ T cells. Proc. Natl. Acad. Sci. USA 95: 8187-8192 [Abstract] [Full Text]
Akashi, K., Kondo, M., Weissman, I. L. (1998). Two distinct pathways of positive selection for thymocytes. Proc. Natl. Acad. Sci. USA 95: 2486-2491 [Abstract] [Full Text]
Page, D. M., Roberts, E. M., Peschon, J. J., Hedrick, S. M. (1998). TNF Receptor-Deficient Mice Reveal Striking Differences Between Several Models of Thymocyte Negative Selection. J. Immunol. 160: 120-133 [Abstract] [Full Text]
Clair, E. G.�St., Anderson, S. J., Oltvai, Z. N. (1997). Bcl-2 Counters Apoptosis by Bax Heterodimerization-dependent and -independent Mechanisms in the T-cell Lineage. J. Biol. Chem. 272: 29347-29355 [Abstract] [Full Text]
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Kishimoto, H., Sprent, J. (1997). Negative Selection in the Thymus Includes Semimature T Cells. JEM 185: 263-272 [Abstract] [Full Text]
Morrison, S., Wandycz, A., Hemmati, H., Wright, D., Weissman, I. (1997). Identification of a lineage of multipotent hematopoietic progenitors. Development 124: 1929-1939 [Abstract]
Guidos, C J, Williams, C J, Grandal, I, Knowles, G, Huang, M T, Danska, J S (1996). V(D)J recombination activates a p53-dependent DNA damage checkpoint in scid lymphocyte precursors.. Genes Dev. 10: 2038-2054 [Abstract]
Danska, J., Pflumio, F, Williams, C., Huner, O, Dick, J., Guidos, C. (1994). Rescue of T cell-specific V(D)J recombination in SCID mice by DNA-damaging agents. Science 266: 450-455 [Abstract]
Sebzda, E, Wallace, V., Mayer, J, Yeung, R., Mak, T., Ohashi, P. (1994). Positive and negative thymocyte selection induced by different concentrations of a single peptide. Science 263: 1615-1618 [Abstract]
van Meerwijk, J., Germain, R. (1993). Development of mature CD8+ thymocytes: selection rather than instruction?. Science 261: 911-915 [Abstract]
Philpott, K., Viney, J., Kay, G, Rastan, S, Gardiner, E., Chae, S, Hayday, A., Owen, M. (1992). Lymphoid development in mice congenitally lacking T cell receptor alpha beta-expressing cells. Science 256: 1448-1452 [Abstract]