Localization of gamma/delta T cells to the intestinal epithelium is independent of normal microbial colonization

doi:10.1084/jem.172.1.239

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Localization of gamma/delta T cells to the intestinal epithelium is independent of normal microbial colonization

A Bandeira, T Mota-Santos, S Itohara, S Degermann, C Heusser, S Tonegawa and A Coutinho
Unite d'Immunobiologie, Institut Pasteur, Paris, France.

Using monoclonal antibodies identifying all gamma/delta and alpha/beta T cell receptors in cytofluorometric analysis, we have compared the composition of intestinal intraepithelial lymphocytes (i-IEL) in euthymic and athymic germ-free (GF) and conventional (SPF) mice. The results show a marked influence of microbial colonization in the numbers of single-positive (CD4+ or CD8+) alpha/beta i-IEL, but little effect in the pool size or characteristics of gamma/delta i-IEL. In young athymic mice, virtually no alpha/beta i-IEL are detected, while considerable numbers of gamma/delta i-IEL remain, though reduced in GF animals.
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