Journal of Experimental Medicine, Vol 171, 2165-2170, Copyright © 1990 by Rockefeller University Press

ARTICLES

Specific erythrocyte binding is an additional nutrient acquisition system for Trichomonas vaginalis

MW Lehker, TH Chang, DC Dailey and JF Alderete
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284.

Specific receptor-mediated binding by Trichomonas vaginalis of human erythrocytes was demonstrated. The ability of live parasites to internalize erythrocytes was also documented. In vitro growth assays during lipid-free and iron-limiting conditions that do not support the survival of T. vaginalis organisms showed that purified erythrocyte lipids and hemoglobin were each able to provide lipids and/or hemoglobin iron for trichomonal growth and multiplication. Parasites bound hemoglobin in a highly specific receptor-mediated fashion, and only the homologous unlabeled hemoglobin, but not lactoferrin and transferrin, competed with iodinated hemoglobin binding. Two antibody- crossreactive surface proteins of the parasites were identified as adhesins, and antibody to the individual adhesins inhibited T. vaginalis recognition and binding of erythrocytes. Finally, patient sera possessed antibody to the adhesins, showing the immunogenic nature and in vivo relevance of the trichomonad proteins during infection.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Tiwari, P., Singh, D., Singh, M. M. (2008). Anti-Trichomonas activity of Sapindus saponins, a candidate for development as microbicidal contraceptive. J Antimicrob Chemother 62: 526-534 [Abstract] [Full Text]  
• Carlton, J. M., Hirt, R. P., Silva, J. C., Delcher, A. L., Schatz, M., Zhao, Q., Wortman, J. R., Bidwell, S. L., Alsmark, U. C. M., Besteiro, S., Sicheritz-Ponten, T., Noel, C. J., Dacks, J. B., Foster, P. G., Simillion, C., Van de Peer, Y., Miranda-Saavedra, D., Barton, G. J., Westrop, G. D., Muller, S., Dessi, D., Fiori, P. L., Ren, Q., Paulsen, I., Zhang, H., Bastida-Corcuera, F. D., Simoes-Barbosa, A., Brown, M. T., Hayes, R. D., Mukherjee, M., Okumura, C. Y., Schneider, R., Smith, A. J., Vanacova, S., Villalvazo, M., Haas, B. J., Pertea, M., Feldblyum, T. V., Utterback, T. R., Shu, C.-L., Osoegawa, K., de Jong, P. J., Hrdy, I., Horvathova, L., Zubacova, Z., Dolezal, P., Malik, S.-B., Logsdon, J. M. Jr., Henze, K., Gupta, A., Wang, C. C., Dunne, R. L., Upcroft, J. A., Upcroft, P., White, O., Salzberg, S. L., Tang, P., Chiu, C.-H., Lee, Y.-S., Embley, T. M., Coombs, G. H., Mottram, J. C., Tachezy, J., Fraser-Liggett, C. M., Johnson, P. J. (2007). Draft Genome Sequence of the Sexually Transmitted Pathogen Trichomonas vaginalis. Science 315: 207-212 [Abstract] [Full Text]  
• Kucknoor, A. S., Mundodi, V., Alderete, J. F. (2005). Adherence to Human Vaginal Epithelial Cells Signals for Increased Expression of Trichomonas vaginalis Genes. Infect. Immun. 73: 6472-6478 [Abstract] [Full Text]  
• Sutak, R., Chamot, C., Tachezy, J., Camadro, J.-M., Lesuisse, E. (2004). Siderophore and haem iron use by Tritrichomonas foetus. Microbiology 150: 3979-3987 [Abstract] [Full Text]  
• Leon-Sicairos, C. R., Leon-Felix, J., Arroyo, R. (2004). tvcp12: a novel Trichomonas vaginalis cathepsin L-like cysteine proteinase-encoding gene. Microbiology 150: 1131-1138 [Abstract] [Full Text]  
• Petrin, D., Delgaty, K., Bhatt, R., Garber, G. (1998). Clinical and Microbiological Aspects of Trichomonas vaginalis. Clin. Microbiol. Rev. 11: 300-317 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS