The Journal of Experimental Medicine
Accuri Cytometers
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Journal of Experimental Medicine, Vol 170, 2171-2176, Copyright © 1989 by Rockefeller University Press

ARTICLES

A single amino acid mutation in a protein antigen abrogates presentation of certain T cell determinants

A Finnegan and CF Amburgey
Department of Internal Medicine, Rush-Presbyterian-St. Luke's Medical School, Chicago, Illinois 60612.

Nase-specific T cell recognize the 86-100 peptide in association with B10.A APC. Clone N40 recognizes the 86-100 peptide in association with B10.A (Ek alpha Ek beta) and B10.A (5R) (Ek alpha Eb beta) APCs. We demonstrate here that a single amino acid substitution in the staphylococcal nuclease protein alters the structure of the processed peptide such that the T cell epitope recognized by clone N40 was only available for recognition in conjunction with B10.A (5R) but not the B10.A APCs. Other Nase-specific T cells recognize the mutant nuclease, and a synthetic peptide corresponding to the immunodominant region of the mutant protein was stimulatory for all the Nase-specific T cells. These results suggest that the mutation either affects the processing of the protein into antigenic peptides or affects the conformation of the processed fragment differently from that of the peptide.
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