Reactivity of V beta 17a+ CD8+ T cell hybrids. Analysis using a new CD8+ T cell fusion partner

doi:10.1084/jem.170.6.1887

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Burgert, H. G.
	Articles by Kappler, J. W.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Burgert, H. G.
	Articles by Kappler, J. W.


Social Bookmarking

	What's this?

ARTICLES

Reactivity of V beta 17a+ CD8+ T cell hybrids. Analysis using a new CD8+ T cell fusion partner

HG Burgert, J White, HU Weltzien, P Marrack and JW Kappler
Howard Hughes Medical Institute, Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.

Tolerance to IE molecules leads to deletion of V beta 17a-bearing T cells. Both, the CD4+ as well as the CD8+ T cell subsets are affected. A large percentage of CD4+ V beta 17a+ T cell hybrids recognize IE molecules. We now have investigated the reactivity for IE antigens of CD8+ V beta 17a+ T cell hybrids. Using a transfection approach, we have introduced the murine CD8 molecule into different V beta 17a+ T cell hybrids. Furthermore, the CD8 cDNA was transfected into the BW5147 alpha-beta- fusion partner. This allowed us to generate a large number of V beta 17a+ T cell hybrids by fusion with the appropriate T cells. Only 6% of T cell hybrids were stimulated to produce IL-2 upon incubation with IE+ cells. However, in those, the CD8 molecule seemed not to contribute to the IE reactivity of the hybrid, since mAbs against the CD8 molecule failed to inhibit their reactivity. This low percentage of V beta 17a+ CD8+ IE-reactive T cell hybrids contrasts with the strong reduction of CD8+ V beta 17a+ T cells in IE+ mice, strongly suggesting that elimination of such cells in the thymus occurs when they are coexpressing CD4 and CD8. This view was confirmed by the occasional expression of CD4 in some hybrids in which case IE reactivity was detected. Furthermore, we demonstrated the functional integrity of the introduced CD8 molecule by: (a) reconstitution of the IL-2 response in a class I-restricted TNP-specific T cell hybrid; and (b) by generation of alloreactive class I-restricted T cell hybrids using the new CD8+ fusion cell line. This CD8+ fusion partner, BWLyt2- 4, should prove useful to study antigen processing and antigen presentation requirements of class I-restricted T cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Hardy, L. L., Wick, D. A., Webb, J. R. (2008). Conversion of Tyrosine to the Inflammation-Associated Analog 3'-Nitrotyrosine at Either TCR- or MHC-Contact Positions Can Profoundly Affect Recognition of the MHC Class I-Restricted Epitope of Lymphocytic Choriomeningitis Virus Glycoprotein 33 by CD8 T Cells. J. Immunol. 180: 5956-5962 [Abstract] [Full Text]
Bagai, R., Valujskikh, A., Canaday, D. H., Bailey, E., Lalli, P. N., Harding, C. V., Heeger, P. S. (2005). Mouse Endothelial Cells Cross-Present Lymphocyte-Derived Antigen on Class I MHC via a TAP1- and Proteasome-Dependent Pathway. J. Immunol. 174: 7711-7715 [Abstract] [Full Text]
Chefalo, P. J., Harding, C. V. (2001). Processing of Exogenous Antigens for Presentation by Class I MHC Molecules Involves Post-Golgi Peptide Exchange Influenced by Peptide-MHC Complex Stability and Acidic pH. J. Immunol. 167: 1274-1282 [Abstract] [Full Text]
White, J., Crawford, F., Fremont, D., Marrack, P., Kappler, J. (1999). Soluble Class I MHC with {beta}2-Microglobulin Covalently Linked Peptides: Specific Binding to a T Cell Hybridoma. J. Immunol. 162: 2671-2676 [Abstract] [Full Text]
Purcell, A. W., Chen, W., Ede, N. J., Gorman, J. J., Fecondo, J. V., Jackson, D. C., Zhao, Y., McCluskey, J. (1998). Avoidance of Self-Reactivity Results in Skewed CTL Responses to Rare Components of Synthetic Immunogens. J. Immunol. 160: 1085-1090 [Abstract] [Full Text]
Sprent, J, Gao, E., Webb, S. (1990). T cell reactivity to MHC molecules: immunity versus tolerance. Science 248: 1357-1363 [Abstract]
Burkly, L., Lo, D, Flavell, R. (1990). Tolerance in transgenic mice expressing major histocompatibility molecules extrathymically on pancreatic cells. Science 248: 1364-1368 [Abstract]