A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions

doi:10.1084/jem.170.2.399

3rd Skeletal Biology and Medicine Symposium

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A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions

WA Muller, CM Ratti, SL McDonnell and ZA Cohn
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021.

We have raised an mAb to a previously undescribed 135-kD externally disposed integral membrane protein that is enriched in the intercellular junctional domain of cultured human umbilical vein endothelial cells. This protein localizes at the appositional surfaces of cells as they become confluent and is stably expressed in the junctional zones of confluent monolayers. This protein is expressed in situ on continuous endothelia of all blood vessels in all human tissues examined. Moreover, this protein, as determined by mAb immunocytochemistry, is not expressed by any other cell type. This protein may mediate endothelial-specific functions restricted to the intercellular domain. It may also serve as a unique cell surface marker for the identification and purification of human endothelial cells.
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Chiba, R., Nakagawa, N., Kurasawa, K., Tanaka, Y., Saito, Y., Iwamoto, I. (1999). Ligation of CD31 (PECAM-1) on Endothelial Cells Increases Adhesive Function of alpha vbeta 3 Integrin and Enhances beta 1 Integrin-Mediated Adhesion of Eosinophils to Endothelial Cells. Blood 94: 1319-1329 [Abstract] [Full Text]
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Gory, S., Vernet, M., Laurent, M., Dejana, E., Dalmon, J., Huber, P. (1999). The Vascular Endothelial-Cadherin Promoter Directs Endothelial-Specific Expression in Transgenic Mice. Blood 93: 184-192 [Abstract] [Full Text]
Randolph, G. J., Luther, T., Albrecht, S., Magdolen, V., Muller, W. A. (1998). Role of Tissue Factor in Adhesion of Mononuclear Phagocytes to and Trafficking Through Endothelium In Vitro. Blood 92: 4167-4177 [Abstract] [Full Text]
Lorenzon, P., Vecile, E., Nardon, E., Ferrero, E., Harlan, J.M., Tedesco, F., Dobrina, A. (1998). Endothelial Cell E- and P-Selectin and Vascular Cell Adhesion Molecule-1 Function as Signaling Receptors. J. Cell Biol. 142: 1381-1391 [Abstract] [Full Text]
Kessel, J. M., Hayflick, J., Weyrich, A. S., Hoffman, P. A., Gallatin, M., McIntyre, T. M., Prescott, S. M., Zimmerman, G. A. (1998). Coengagement of ICAM-3 and Fc Receptors Induces Chemokine Secretion and Spreading by Myeloid Leukocytes. J. Immunol. 160: 5579-5587 [Abstract] [Full Text]
Nakashima, Y., Raines, E. W., Plump, A. S., Breslow, J. L., Ross, R. (1998). Upregulation of VCAM-1 and ICAM-1 at Atherosclerosis-Prone Sites on the Endothelium in the ApoE-Deficient Mouse. Arterioscler. Thromb. Vasc. Bio. 18: 842-851 [Abstract] [Full Text]
Allport, J. R., Ding, H., Collins, T., Gerritsen, M. E., Luscinskas, F. W. (1997). Endothelial-dependent Mechanisms Regulate Leukocyte Transmigration: A Process Involving the Proteasome and Disruption of the Vascular Endothelial-Cadherin Complex at Endothelial Cell-to-Cell Junctions. J. Exp. Med. 186: 517-527 [Abstract] [Full Text]
Iwaki, K., Ohashi, K., Ikeda, M., Tsujioka, K., Kajiya, F., Kurimoto, M. (1997). Decrease in the Amount of Focal Adhesion Kinase (p125FAK) in Interleukin-1beta -stimulated Human Umbilical Vein Endothelial Cells by Binding of Human Monocytic Cell Lines. J. Biol. Chem. 272: 20665-20670 [Abstract] [Full Text]
Liao, F., Ali, J., Greene, T., Muller, W. A. (1997). Soluble Domain 1�of Platelet-Endothelial Cell Adhesion Molecule (PECAM) Is Sufficient to Block Transendothelial Migration In Vitro and In Vivo. J. Exp. Med. 185: 1349-1358 [Abstract] [Full Text]
Sun, J., Williams, J., Yan, H.-C., Amin, K. M., Albelda, S. M., DeLisser, H. M. (1996). Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) Homophilic Adhesion Is Mediated by Immunoglobulin-like Domains 1and 2and Depends on the Cytoplasmic Domain and the Level of Surface Expression. J. Biol. Chem. 271: 18561-18570 [Abstract] [Full Text]
Sun, Q.-H., DeLisser, H. M., Zukowski, M. M., Paddock, C., Albelda, S. M., Newman, P. J. (1996). Individually Distinct Ig Homology Domains in PECAM-1 Regulate Homophilic Binding and Modulate Receptor Affinity. J. Biol. Chem. 271: 11090-11098 [Abstract] [Full Text]
Buckley, C., Doyonnas, R, Newton, J., Blystone, S., Brown, E., Watt, S., Simmons, D. (1996). Identification of alpha v beta 3 as a heterotypic ligand for CD31/PECAM-1. J. Cell Sci. 109: 437-445 [Abstract]
Breviario, F., Caveda, L., Corada, M., Martin-Padura, I., Navarro, P., Golay, J., Introna, M., Gulino, D., Lampugnani, M. G., Dejana, E. (1995). Functional Properties of Human Vascular Endothelial Cadherin (7B4/Cadherin-5), an Endothelium-Specific Cadherin. Arterioscler. Thromb. Vasc. Bio. 15: 1229-1239 [Abstract] [Full Text]
Tang, D., Diglio, C., Bazaz, R, Honn, K. (1995). Transcriptional activation of endothelial cell integrin alpha v by protein kinase C activator 12(S)-HETE. J. Cell Sci. 108: 2629-2644 [Abstract]
Roberts, W., Palade, G. (1995). Increased microvascular permeability and endothelial fenestration induced by vascular endothelial growth factor. J. Cell Sci. 108: 2369-2379 [Abstract]
Kwee, L, Baldwin, H., Shen, H., Stewart, C., Buck, C, Buck, C., Labow, M. (1995). Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice. Development 121: 489-503 [Abstract]
Baldwin, H. S., Shen, H. M., Yan, H. C., DeLisser, H. M., Chung, A., Mickanin, C., Trask, T., Kirschbaum, N. E., Newman, P. J., Albelda, S. M., et, al. (1994). Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development 120: 2539-2553 [Abstract]
Leach, L, Clark, P, Lampugnani, M., Arroyo, A., Dejana, E, Firth, J. (1993). Immunoelectron characterisation of the inter-endothelial junctions of human term placenta. J. Cell Sci. 104: 1073-1081 [Abstract]
Newman, P., Berndt, M., Gorski, J, White, G. 2nd, Lyman, S, Paddock, C, Muller, W. (1990). PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247: 1219-1222 [Abstract]
Cicmil, M., Thomas, J. M., Sage, T., Barry, F. A., Leduc, M., Bon, C., Gibbins, J. M. (2000). Collagen, Convulxin, and Thrombin Stimulate Aggregation-independent Tyrosine Phosphorylation of CD31 in Platelets. EVIDENCE FOR THE INVOLVEMENT OF Src FAMILY KINASES. J. Biol. Chem. 275: 27339-27347 [Abstract] [Full Text]