Journal of Experimental Medicine, Vol 170, 339-342, Copyright © 1989 by Rockefeller University Press

ARTICLES

Demonstration of delta rec-pseudo J alpha rearrangement with deletion of the delta locus in a human stem-cell leukemia

CG Begley, PD Aplan, MP Davey, JP de Villartay, DI Cohen, TA Waldmann and IR Kirsch
Navy Medical Oncology, National Cancer Institute, Bethesda, Maryland 20892.

It has been hypothesized that a rearrangement between the delta recombining element (delta Rec) and a pseudo J alpha gene serves to delete the TCR-delta locus before rearrangement of the TCR-alpha genes. We have now sequenced a direct, site-specific rearrangement between the delta Rec element and a pseudo J alpha gene in a human leukemic stem- cell line. Putative "N-sequence" addition was noted at the site of recombination, suggesting that this event occurred at a time when the enzyme(s) involved in N-region addition were active in this cell. This provides support for the view that deletion of the TCR-delta locus is required before rearrangement of the TCR-alpha chain genes.
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This article has been cited by other articles:

• Wang, J., Jani-Sait, S. N., Escalon, E. A., Carroll, A. J., de Jong, P. J., Kirsch, I. R., Aplan, P. D. (2000). The t(14;21)(q11.2;q22) chromosomal translocation associated with T-cell acute lymphoblastic leukemia activates the BHLHB1 gene. Proc. Natl. Acad. Sci. USA 97: 3497-3502 [Abstract] [Full Text]  
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