The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 169, 351-356, Copyright © 1989 by Rockefeller University Press

ARTICLES

Modulation of HLA class II antigen expression by transfection of sense and antisense DR alpha cDNA

V Lotteau, J Sands, L Teyton, P Turmel, D Charron and JL Strominger
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

In the human there are three isotypic forms of MHC class II gene products (HLA-DR, -DQ, and -DP). The isotype-matched alpha-beta dimers are predominant but isotype-mismatched dimers can also be expressed (DR alpha-DQ beta). Here it is shown that the expression of the DR alpha-DQ beta dimer can be correlated to a high ratio of DR alpha/DR beta mRNA. The DR alpha chain expression was modulated by transfection of a sense and antisense DR alpha cDNA. Overexpression of DR alpha promoted the appearance of the DR alpha-DQ beta dimer. On the other hand, pre- existing DR alpha-DQ beta dimer expression was suppressed after antisense DR alpha cDNA transfection. Therefore, imbalanced expression of the alpha and beta chain from a given isotype could lead to the modification of HLA class II phenotype.
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